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ID   PYAC5      preliminary; circular DNA; SYN; 11454 BP.
XX
AC   IG0333; U01086;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli YAC vector pYAC5 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from YIp5
RC   pPM662 from plasmid & YCp19
RC   pPM664 from pPM662 & linker & pSU4-A, sup4-o gene
RC   pPM668 from pPM664
RC   pYAC2 from pPM668 & linker & A240p1
RC   pYAC3 from pYAC2
RC   pYAC4 from pYAC3 & linker
RC   pYAC5, pYAC55 from pYAC3 & linker
RA   Burke D.T., Carle G.F., Olson M.V.;
RT   "Cloning of large segments of exogenous DNA into yeast by means of
RT   artificial chromosome vectors";
RL   Science 236:806-812(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   old Sigma vector.
CC   NM (pYAC5)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (yeast artificial chromosome)
CC   SP ()
CC   HO (E.coli)(yeast ade2-ochre)(ATCC 20843)
CC   CP ()
CC   FN (cloning 50000-100000 bp)
CC   SE (color red/white)
CC   PA (YIp5)(YCp19)(yeast sup4-o gene)(lambda)(yeast telomere)
CC   BR (pYAC4)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pYAC3 SnaBI 11420bp 554..554, suf4 [11170bp]
FT                   NotI linker 8bp gcggccgc
FT                   -> pYAC5 11428bp [11700bp]"
FT   -               1..552
FT                   /note="pYAC4 1..552 552bp
FT                   SnaBI = TAC^GTA
FT                   \           gcggccgc"
FT   -               553..560
FT                   /note="gcggccgc 8bp
FT                   \  gcggccgc
FT                   SnaBI = TAC^GTA"
FT   -               561..11454
FT                   /note="pYAC4 561..11454 10894bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI (makes circular into linear)"
FT   misc_binding    0..0
FT                   /note="SIT unique ClaI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN4 (for chromosome
FT                   segregation and stability)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 (tryptophan)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   CDS             0..0
FT                   /note="ANT yeast SUP4 gene (ochre suppressor)"
FT   misc_binding    0..0
FT                   /note="SIT unique NotI"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene (uracil)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 11454 BP; 3148 A; 2552 C; 2568 G; 3186 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatca attcccttta gtataaattt cactctgaac catcttggaa
     ggaccggtaa ttatttcaaa tctctttttc aattgtatat gtgttatgtt atgtagtata
     ctctttcttc aacaattaaa tactctcggt agccaagttg gtttaaggcg caagacttta
     atttatcact acgcggccgc gtaatcttga gatcgggcgt tcgatcgccc cgggagattt
     ttttgttttt tatgtcttcc attcacttcc cagacttgca agttgaaata tttctttcaa
     gggaattgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg atgcccttga
     gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg
     tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg
     ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct
     cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc
     caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat
     cgcgtccgcc atctccagca gccgcacgcg gcgcatcccc cccccccttt caattcaatt
     catcattttt tttttattct tttttttgat ttcggtttct ttgaaatttt tttgattcgg
     taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac
     gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa
     caaaaacctg caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg
     ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa
     acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat
     taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg
     agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag
     acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca
     gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta
     gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag
     cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca
     ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa
     gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag
     acgcattggg tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta
     ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt
     acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg
     tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
     agttattact cgggcgtaat gatttttata atgacgaaaa aaaaaaaatt ggaaagaaaa
     gggggggggg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg
     aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc
     gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg
     gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
     atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
     ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
     gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
     atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaattccccc
     ttacacggag gcatcaagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
     cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc cctcgaggga
     taagcttcat ttttagataa aatttattaa tcatcattaa tttcttgaaa aacattttat
     ttattgatct tttataacaa aaaacccttc taaaagttta tttttgaatg aaaaacttat
     aaaaatttat gaaaactaca aaaaataaaa tttttaatta aaataatttt gataagaact
     tcaatctttg actagctagc ttagtcattt ttgagattta attaatattt tatgtttatt
     catatataaa ctattcaaaa tattatagaa tttaaacatt ttaacatctt aatcattcat
     aaataactaa aaatcaaagt attacatcaa taaataactt ttactcaatg tcaaagaatt
     attggggttg gggttggggt tggggttggg gttggggttg gggttggggt tggggttggg
     gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt tggggttggg
     gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt tggggttggg
     gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt tggggttggg
     gttggggttg gggttggggt tggggttggg gtgggaaaac agcattcagg tattagaaga
     atatcctgat tcaggtgaaa atattgttga tgcgcgggat cctcggggac accaaatatg
     gcgatctcgg ccttttcgtt tcttggagct gggacatgtt tgccatcgat ccatctacca
     ccagaacggc cgttagatct gctgccaccg ttgtttccac cgaagaaacc accgttgccg
     taaccaccac gacggttgtt gctaaagaag ctgccaccgc cacggccacc gttgtagccg
     ccgttgttgt tattgtagtt gctcatgtta tttctggcac ttcttggttt tcctcttaag
     tgaggaggaa cataaccatt ctcgttgttg tcgttgatgc ttaaattttg cacttgttcg
     ctcagttcag ccataatatg aaatgctttt cttgttgttc ttacggaata ccacttgcca
     cctatcacca caactaactt tttcccgttc ctccatctct tttatatttt ttttctcgat
     cgagttcaag agaaaaaaaa agaaaaagca aaaagaaaaa aggaaagcgc gcctcgttca
     gaatgacacg tatagaatga tgcattacct tgtcatcttc agtatcatac tgttcgtata
     catacttact gacattcata ggtatacata tatacacatg tatatatatc gtatgctgca
     gctttaaata atcggtgtca ctacataaga acacctttgg tggagggaac atcgttggta
     ccattgggcg aggtggcttc tcttatggca accgcaagag ccttgaacgc actctcacta
     cggtgatgat cattcttgcc tcgcagacaa tcaacgtgga gggtaattct gctagcctct
     gcaaagcttt caagaaaatg cgggatcatc tcgcaagaga gatctcctac tttctccctt
     tgcaaaccaa gttcgacaac tgcgtacggc ctgttcgaaa gatctaccac cgctctggaa
     agtgcctcat ccaaaggcgc aaatcctgat ccaaaccttt ttactccacg cgccagtagg
     gcctctttaa aagcttgacc gagagcaatc ccgcagtctt cagtggtgtg atggtcgtct
     atgtgtaagt caccaatgca ctcaacgatt agcgaccagc cggaatgctt ggccagagca
     tgtatcatat ggtccagaaa ccctatacct gtgtggacgt taatcacttg cgattgtgtg
     gcctgttctg ctactgcttc tgcctctttt tctgggaaga tcgagtgctc tatcgctagg
     ggaccaccct ttaaagagat cgcaatctga atcttggttt catttgtaat acgctttact
     agggctttct gctctgtcat ctttgccttc gtttatcttg cctgctcatt ttttagtata
     ttcttcgaag aaatcacatt actttatata atgtataatt cattatgtga taatgccaat
     cgctaagaaa aaaaaagagt catccgctag gtggaaaaaa aaaaatgaaa atcattaccg
     aggcataaaa aaatatagag tgtactagag gaggccaaga gtaatagaaa aagaaaattg
     cgggaaagga ctgtgttatg acttccctga ctaatgccgt gttcaaacga tacctggcag
     tgactcctag cgctcaccaa gctcttaaaa cgagaattaa gaaaaagtcg tcatctttcg
     ataagttttt cccacagcaa agcaatagta gaaaaaaaca atgggaaacg ttgaatgaag
     acaaagcgtc gtggtttaaa aggaaatacg ctcacgtaca tgctagggaa caggaccgtg
     cagcggatcc cgcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     aatgctgttt tcccacccca accccaaccc caaccccaac cccaacccca accccaaccc
     caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca accccaaccc
     caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca accccaaccc
     caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca accccaaccc
     caaccccaac cccaacccca accccaaccc caaccccaac cccaataatt ctttgacatt
     gagtaaaagt tatttattga tgtaatactt tgatttttag ttatttatga atgattaaga
     tgttaaaatg tttaaattct ataatatttt gaatagttta tatatgaata aacataaaat
     attaattaaa tctcaaaaat gactaagcta gctagtcaaa gattgaagtt cttatcaaaa
     ttattttaat taaaaatttt attttttgta gttttcataa atttttataa gtttttcatt
     caaaaataaa cttttagaag ggttttttgt tataaaagat caataaataa aatgtttttc
     aagaaattaa tgatgattaa taaattttat ctaaaaatga agcttatccc tcgagggctg
     cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
     tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
     actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
     tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     cgtcttcaag aattaattcg gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt
     ggtgactatt gagcacgtga gtatacgtga ttaagcacac aaaggcagct tggagtatgt
     ctgttattaa tttcacaggt agttctggtc cattggtgaa agtttgcggc ttgcagagca
     cagaggccgc agaatgtgct ctagattccg atgctgactt gctgggtatt atatgtgtgc
     ccaatagaaa gagaacaatt gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag
     catataaaaa tagttcaggc actccgaaat acttggttgg cgtgtttcgt aatcaaccta
     aggaggatgt tttggctctg gtcaatgatt acggcattga tatcgtccaa ctgcatggag
     atgagtcgtg gcaagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat
     ttccaaaaga ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc
     ccttgtttga ttcagaagca ggtgggacag gtgaactttt ggattggaac tcgatttctg
     actgggttgg aaggcaagag agccccgaaa gcttacattt tatgttagct ggtggactga
     cgccagaaaa tgttggtgat gcgcttagat taaatggcgt tattggtgtt gatgtaagcg
     gaggtgtgga gacaaatggt gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg
     ctaagaaata ggttattact gagtagtatt tatttaagta ttgtttgtgc acttgcctgc
     aggccttttg aaaagcaagc ataaaagatc taaacataaa atctgtaaaa taacaagatg
     taaagataat gctaaatcat ttggcttttt gattgattgt acaggaaaat atacatcgca
     gggggttgac ttttaccatt tcaccgcaat ggaatcaaac ttgttgaaga gaatgttcac
     aggcgcatac gctacaatga cccgattctt gctagccttt tctcggtctt gcaaacaacc
     gccggcagct tagtatataa atacacatgt acatacctct ctccgtatcc tcgtaatcat
     tttcttgtat ttatcgtctt ttcgctgtaa aaactttatc acacttatct caaatacact
     tattaaccgc ttttactatt atcttctacg ctgacagtaa tatcaaacag tgacacatat
     taaacacagt ggtttctttg cataaacacc atcagcctca agtcgtcaag taaagatttc
     gtgttcatgc agatagataa caatctatat gttgataatt agcgttgcct catcaatgcg
     agatccgttt aaccggaccc tagtgcactt accccacgtt cggtccactg tgtgccgaac
     atgctccttc actattttaa catgtggaat taattctaaa tcctctttat atgatctgcc
     gatagatagt tctaagtcat tgaggttcat caacaattgg attttctgtt tactcgactt
     caggtaaatg aaatgagatg atacttgctt atctcatagt taactctaag aggtgatact
     tatttactgt aaaactgtga cgataaaacc ggaaggaaga ataagaaaac tcgaactgat
     ctataatgcc tattttctgt aaagagttta agctatgaaa gcctcggcat tttggccgct
     cctaggtagt gctttttttc caaggacaaa acagtttctt tttcttgagc aggttttatg
     tttcggtaat cataaacaat aaataaatta tttcatttat gtttaaaaat aaaaaataaa
     aaagtatttt aaatttttaa aaaagttgat tataagcatg tgaccttttg caagcaatta
     aattttgcaa tttgtgattt taggcaaaag ttacaatttc tggctcgtgt aatatatgta
     tgctaaagtg aacttttaca aagtcgatat ggacttagtc aaaagaaatt ttcttaaaaa
     tatatagcac tagccaattt agcacttctt tatgagatat attatagact ttattaagcc
     agatttgtgt attatatgta tttacccggc gaatcatgga catacattct gaaataggta
     atattctcta tggtgagaca gcatagataa cctaggatac aagttaaaag ctagtactgt
     tttgcagtaa tttttttctt ttttataaga atgttaccac ctaaataagt tataaagtca
     atagttaagt ttgatatttg attgtaaaat accgtaatat atttgcatga tcaaaaggct
     caatgttgac tagccagcat gtcaaccact atattgatca ccgatatatg gacttccaca
     ccaactagta atatgacaat aaattcaaga tattcttcat gagaatggcc caga
//