Return to this vector's summary.
ID PYAC5 preliminary; circular DNA; SYN; 11454 BP.
XX
AC IG0333; U01086;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Saccharomyces/E.coli YAC vector pYAC5 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC plasmid from YIp5
RC pPM662 from plasmid & YCp19
RC pPM664 from pPM662 & linker & pSU4-A, sup4-o gene
RC pPM668 from pPM664
RC pYAC2 from pPM668 & linker & A240p1
RC pYAC3 from pYAC2
RC pYAC4 from pYAC3 & linker
RC pYAC5, pYAC55 from pYAC3 & linker
RA Burke D.T., Carle G.F., Olson M.V.;
RT "Cloning of large segments of exogenous DNA into yeast by means of
RT artificial chromosome vectors";
RL Science 236:806-812(1987).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC old Sigma vector.
CC NM (pYAC5)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (yeast artificial chromosome)
CC SP ()
CC HO (E.coli)(yeast ade2-ochre)(ATCC 20843)
CC CP ()
CC FN (cloning 50000-100000 bp)
CC SE (color red/white)
CC PA (YIp5)(YCp19)(yeast sup4-o gene)(lambda)(yeast telomere)
CC BR (pYAC4)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pYAC3 SnaBI 11420bp 554..554, suf4 [11170bp]
FT NotI linker 8bp gcggccgc
FT -> pYAC5 11428bp [11700bp]"
FT - 1..552
FT /note="pYAC4 1..552 552bp
FT SnaBI = TAC^GTA
FT \ gcggccgc"
FT - 553..560
FT /note="gcggccgc 8bp
FT \ gcggccgc
FT SnaBI = TAC^GTA"
FT - 561..11454
FT /note="pYAC4 561..11454 10894bp"
FT misc_binding 0..0
FT /note="SIT unique BamHI (makes circular into linear)"
FT misc_binding 0..0
FT /note="SIT unique ClaI"
FT misc_binding 0..0
FT /note="SIT unique SalI"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_feature 0..0
FT /note="yeast centromere CEN4 (for chromosome
FT segregation and stability)"
FT CDS 0..0
FT /note="ANT yeast TRP1 (tryptophan)"
FT rep_origin 0..0
FT /note="ORI yeast ARS1"
FT CDS 0..0
FT /note="ANT yeast SUP4 gene (ochre suppressor)"
FT misc_binding 0..0
FT /note="SIT unique NotI"
FT CDS 0..0
FT /note="ANT yeast URA3 gene (uracil)"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ Sequence 11454 BP; 3148 A; 2552 C; 2568 G; 3186 T; 0 other;
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
cacacccgtc ctgtggatca attcccttta gtataaattt cactctgaac catcttggaa
ggaccggtaa ttatttcaaa tctctttttc aattgtatat gtgttatgtt atgtagtata
ctctttcttc aacaattaaa tactctcggt agccaagttg gtttaaggcg caagacttta
atttatcact acgcggccgc gtaatcttga gatcgggcgt tcgatcgccc cgggagattt
ttttgttttt tatgtcttcc attcacttcc cagacttgca agttgaaata tttctttcaa
gggaattgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg atgcccttga
gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg
tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg
ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct
cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc
caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat
cgcgtccgcc atctccagca gccgcacgcg gcgcatcccc cccccccttt caattcaatt
catcattttt tttttattct tttttttgat ttcggtttct ttgaaatttt tttgattcgg
taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac
gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa
caaaaacctg caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg
ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa
acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat
taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg
agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag
acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca
gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta
gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag
cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca
ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa
gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag
acgcattggg tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta
ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt
acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg
tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
agttattact cgggcgtaat gatttttata atgacgaaaa aaaaaaaatt ggaaagaaaa
gggggggggg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg
aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc
gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg
gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaattccccc
ttacacggag gcatcaagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc cctcgaggga
taagcttcat ttttagataa aatttattaa tcatcattaa tttcttgaaa aacattttat
ttattgatct tttataacaa aaaacccttc taaaagttta tttttgaatg aaaaacttat
aaaaatttat gaaaactaca aaaaataaaa tttttaatta aaataatttt gataagaact
tcaatctttg actagctagc ttagtcattt ttgagattta attaatattt tatgtttatt
catatataaa ctattcaaaa tattatagaa tttaaacatt ttaacatctt aatcattcat
aaataactaa aaatcaaagt attacatcaa taaataactt ttactcaatg tcaaagaatt
attggggttg gggttggggt tggggttggg gttggggttg gggttggggt tggggttggg
gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt tggggttggg
gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt tggggttggg
gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt tggggttggg
gttggggttg gggttggggt tggggttggg gtgggaaaac agcattcagg tattagaaga
atatcctgat tcaggtgaaa atattgttga tgcgcgggat cctcggggac accaaatatg
gcgatctcgg ccttttcgtt tcttggagct gggacatgtt tgccatcgat ccatctacca
ccagaacggc cgttagatct gctgccaccg ttgtttccac cgaagaaacc accgttgccg
taaccaccac gacggttgtt gctaaagaag ctgccaccgc cacggccacc gttgtagccg
ccgttgttgt tattgtagtt gctcatgtta tttctggcac ttcttggttt tcctcttaag
tgaggaggaa cataaccatt ctcgttgttg tcgttgatgc ttaaattttg cacttgttcg
ctcagttcag ccataatatg aaatgctttt cttgttgttc ttacggaata ccacttgcca
cctatcacca caactaactt tttcccgttc ctccatctct tttatatttt ttttctcgat
cgagttcaag agaaaaaaaa agaaaaagca aaaagaaaaa aggaaagcgc gcctcgttca
gaatgacacg tatagaatga tgcattacct tgtcatcttc agtatcatac tgttcgtata
catacttact gacattcata ggtatacata tatacacatg tatatatatc gtatgctgca
gctttaaata atcggtgtca ctacataaga acacctttgg tggagggaac atcgttggta
ccattgggcg aggtggcttc tcttatggca accgcaagag ccttgaacgc actctcacta
cggtgatgat cattcttgcc tcgcagacaa tcaacgtgga gggtaattct gctagcctct
gcaaagcttt caagaaaatg cgggatcatc tcgcaagaga gatctcctac tttctccctt
tgcaaaccaa gttcgacaac tgcgtacggc ctgttcgaaa gatctaccac cgctctggaa
agtgcctcat ccaaaggcgc aaatcctgat ccaaaccttt ttactccacg cgccagtagg
gcctctttaa aagcttgacc gagagcaatc ccgcagtctt cagtggtgtg atggtcgtct
atgtgtaagt caccaatgca ctcaacgatt agcgaccagc cggaatgctt ggccagagca
tgtatcatat ggtccagaaa ccctatacct gtgtggacgt taatcacttg cgattgtgtg
gcctgttctg ctactgcttc tgcctctttt tctgggaaga tcgagtgctc tatcgctagg
ggaccaccct ttaaagagat cgcaatctga atcttggttt catttgtaat acgctttact
agggctttct gctctgtcat ctttgccttc gtttatcttg cctgctcatt ttttagtata
ttcttcgaag aaatcacatt actttatata atgtataatt cattatgtga taatgccaat
cgctaagaaa aaaaaagagt catccgctag gtggaaaaaa aaaaatgaaa atcattaccg
aggcataaaa aaatatagag tgtactagag gaggccaaga gtaatagaaa aagaaaattg
cgggaaagga ctgtgttatg acttccctga ctaatgccgt gttcaaacga tacctggcag
tgactcctag cgctcaccaa gctcttaaaa cgagaattaa gaaaaagtcg tcatctttcg
ataagttttt cccacagcaa agcaatagta gaaaaaaaca atgggaaacg ttgaatgaag
acaaagcgtc gtggtttaaa aggaaatacg ctcacgtaca tgctagggaa caggaccgtg
cagcggatcc cgcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
aatgctgttt tcccacccca accccaaccc caaccccaac cccaacccca accccaaccc
caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca accccaaccc
caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca accccaaccc
caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca accccaaccc
caaccccaac cccaacccca accccaaccc caaccccaac cccaataatt ctttgacatt
gagtaaaagt tatttattga tgtaatactt tgatttttag ttatttatga atgattaaga
tgttaaaatg tttaaattct ataatatttt gaatagttta tatatgaata aacataaaat
attaattaaa tctcaaaaat gactaagcta gctagtcaaa gattgaagtt cttatcaaaa
ttattttaat taaaaatttt attttttgta gttttcataa atttttataa gtttttcatt
caaaaataaa cttttagaag ggttttttgt tataaaagat caataaataa aatgtttttc
aagaaattaa tgatgattaa taaattttat ctaaaaatga agcttatccc tcgagggctg
cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
cgtcttcaag aattaattcg gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt
ggtgactatt gagcacgtga gtatacgtga ttaagcacac aaaggcagct tggagtatgt
ctgttattaa tttcacaggt agttctggtc cattggtgaa agtttgcggc ttgcagagca
cagaggccgc agaatgtgct ctagattccg atgctgactt gctgggtatt atatgtgtgc
ccaatagaaa gagaacaatt gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag
catataaaaa tagttcaggc actccgaaat acttggttgg cgtgtttcgt aatcaaccta
aggaggatgt tttggctctg gtcaatgatt acggcattga tatcgtccaa ctgcatggag
atgagtcgtg gcaagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat
ttccaaaaga ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc
ccttgtttga ttcagaagca ggtgggacag gtgaactttt ggattggaac tcgatttctg
actgggttgg aaggcaagag agccccgaaa gcttacattt tatgttagct ggtggactga
cgccagaaaa tgttggtgat gcgcttagat taaatggcgt tattggtgtt gatgtaagcg
gaggtgtgga gacaaatggt gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg
ctaagaaata ggttattact gagtagtatt tatttaagta ttgtttgtgc acttgcctgc
aggccttttg aaaagcaagc ataaaagatc taaacataaa atctgtaaaa taacaagatg
taaagataat gctaaatcat ttggcttttt gattgattgt acaggaaaat atacatcgca
gggggttgac ttttaccatt tcaccgcaat ggaatcaaac ttgttgaaga gaatgttcac
aggcgcatac gctacaatga cccgattctt gctagccttt tctcggtctt gcaaacaacc
gccggcagct tagtatataa atacacatgt acatacctct ctccgtatcc tcgtaatcat
tttcttgtat ttatcgtctt ttcgctgtaa aaactttatc acacttatct caaatacact
tattaaccgc ttttactatt atcttctacg ctgacagtaa tatcaaacag tgacacatat
taaacacagt ggtttctttg cataaacacc atcagcctca agtcgtcaag taaagatttc
gtgttcatgc agatagataa caatctatat gttgataatt agcgttgcct catcaatgcg
agatccgttt aaccggaccc tagtgcactt accccacgtt cggtccactg tgtgccgaac
atgctccttc actattttaa catgtggaat taattctaaa tcctctttat atgatctgcc
gatagatagt tctaagtcat tgaggttcat caacaattgg attttctgtt tactcgactt
caggtaaatg aaatgagatg atacttgctt atctcatagt taactctaag aggtgatact
tatttactgt aaaactgtga cgataaaacc ggaaggaaga ataagaaaac tcgaactgat
ctataatgcc tattttctgt aaagagttta agctatgaaa gcctcggcat tttggccgct
cctaggtagt gctttttttc caaggacaaa acagtttctt tttcttgagc aggttttatg
tttcggtaat cataaacaat aaataaatta tttcatttat gtttaaaaat aaaaaataaa
aaagtatttt aaatttttaa aaaagttgat tataagcatg tgaccttttg caagcaatta
aattttgcaa tttgtgattt taggcaaaag ttacaatttc tggctcgtgt aatatatgta
tgctaaagtg aacttttaca aagtcgatat ggacttagtc aaaagaaatt ttcttaaaaa
tatatagcac tagccaattt agcacttctt tatgagatat attatagact ttattaagcc
agatttgtgt attatatgta tttacccggc gaatcatgga catacattct gaaataggta
atattctcta tggtgagaca gcatagataa cctaggatac aagttaaaag ctagtactgt
tttgcagtaa tttttttctt ttttataaga atgttaccac ctaaataagt tataaagtca
atagttaagt ttgatatttg attgtaaaat accgtaatat atttgcatga tcaaaaggct
caatgttgac tagccagcat gtcaaccact atattgatca ccgatatatg gacttccaca
ccaactagta atatgacaat aaattcaaga tattcttcat gagaatggcc caga
//