Return to this vector's summary.
ID PYACRC preliminary; circular DNA; SYN; 11487 BP.
XX
AC ATCC37610;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Saccharomyces/E.coli YAC vector pYAC-RC - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pYAC-RC from pYAC3 & linker
RA Marchuk D., Collins F.S.;
RT "pYAC-RC, a yeast artificial chromosome vector for cloning DNA cut
RT with infrequently cutting restriction endonucleases";
RL Nucleic Acids Res. 16:7743-7743(1988).
XX
RN [2]
RC yHPRT from yeast YAC & human HPRT gene
RA Jakobovits A., Moore A.L., Green L.L., Vergara G.J.,
RA Maynard-Curtis C.E., Austin H.A., Klapholz S.;
RT "Germ-line transmission and expression of a human-derived yeast
RT artificial chromosome";
RL Nature 362:255-258(1993).
XX
RN [3]
RC pJEF742 from yeast Ty repeat
RA Boeke J.;
RT ;
RL Unpublished (1993).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC Due to ligation compatibility with sites in the polylinker, DNA
CC fragments from BssHII, EagI, NaeI, NarI, NruI, SmaI and XhoI digests
CC may also be cloned in this vector.
CC Constructed by removing the ClaI and SalI sites from pYAC3 and
CC inserting a polylinker in the SnaBI site within SUP4. SUP4 still
CC functions.
CC A yeast artificial chromosome vector for genomic library construction
CC using DNA digested with infrequent restriction enzymes.
CC Restriction digests of the clone give the following sizes (kb):
CC HindIII--3.7, 3.0, 1.9, 1.47; SalI--12.0. (ATCC staff)
CC Digestion with HindIII gives the following sizes (kb): 3.5, 3.0, 1.9,
CC 1.4(doublet), small fragment. If any telomere DNA has been deleted,
CC the doublet will be resolved and a new band will appear. (personal
CC communication)
CC An occasional isolate will spontaneously delete small amounts of
CC telomere sequence. Check by digesting the plasmid with HindIII.
CC (personal communication)
CC Medium is 1227 LB plus ampicillin.
CC NM (pYAC-RC)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (yeast artificial chromosome)
CC SP (ATCC)
CC HO (E.coli MC1061)(Saccharomyces cerevisiae)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pYAC3)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pYAC3 ClaI 11448bp 25..25, may be 4307..4307
FT \ [11700bp]
FT fill in
FT SalI 11452bp 944..944
FT fill in
FT SnaBI 11460bp 554..554, SUP4 intron
FT 2. linker NotI-SacII-SalI-MluI-ClaI-SnaBI 35bp
FT \ ctacgcggccgcggtcgacgcgtatcgatacgtaa
FT -> pYAC-RC 11495bp [11700bp]"
FT - 1..26
FT /note="pYAC4 1..26 26bp
FT ClaI = AT^CG AT
FT ClaI = AT^CGAT"
FT - 27..554
FT /note="pYAC4 25..552 528bp
FT SnaBI = TAC^GTA
FT \ ctacgcg..."
FT - 555..589
FT /note="35bp
FT \ ctacgcggccgcggtcgacgcgtatcgatacgtaa
FT \ ...acgtaa
FT SnaBI = TAC^GTA"
FT - 590..976
FT /note="pYAC4 561..947 387bp
FT SalI = G^TCGA C
FT SalI = G^TCGAC"
FT - 977..11487
FT /note="pYAC4 944..11454 10511bp"
FT misc_binding 0..0
FT /note="MCS NotI-SacII-SalI-MluI-ClaI-SnaBI-SmaI"
FT misc_binding 0..0
FT /note="SIT unique NotI-SacII-SalI-MluI-ClaI-SnaBI-
FT SmaI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI yeast ARS1"
FT misc_feature 0..0
FT /note="yeast centromere CEN4"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="ANT yeast HIS3 gene"
FT CDS 0..0
FT /note="ANT yeast SUP4 gene (ochre suppressor)"
FT CDS 0..0
FT /note="ANT yeast TRP1 gene (tryptophan)"
FT CDS 0..0
FT /note="ANT yeast URA3 gene"
XX
SQ Sequence 11487 BP; 3156 A; 2561 C; 2577 G; 3193 T; 0 other;
ttctcatgtt tgacagctta tcatcgcgat aagctttaat gcggtagttt atcacagtta
aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc
ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc
ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta
tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc
cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg
accacacccg tcctgtggat caattccctt tagtataaat ttcactctga accatcttgg
aaggaccggt aattatttca aatctctttt tcaattgtat atgtgttatg ttatgtagta
tactctttct tcaacaatta aatactctcg gtagccaagt tggtttaagg cgcaagactt
taatttatca ctacctacgc ggccgcggtc gacgcgtatc gatacgtaag taatcttgag
atcgggcgtt cgatcgcccc gggagatttt tttgtttttt atgtcttcca ttcacttccc
agacttgcaa gttgaaatat ttctttcaag ggaattgatc ctctacgccg gacgcatcgt
ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
taagggagag cgtcgatcga ccgatgccct tgagagcctt caacccagtc agctccttcc
ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga
gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg
cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct
cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa
tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac
gcggcgcatc cccccccccc tttcaattca attcatcatt ttttttttat tctttttttt
gatttcggtt tctttgaaat ttttttgatt cggtaatctc cgaacagaag gaagaacgaa
ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa gaaacatgaa
attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa
tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct gttgctgcca
agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta
ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt ttactaaaaa
cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg ctaaaggcat
tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac attggtaata
cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca gacattacga
atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg gcagaagaag
taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag ggctccctat
ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa gattttgtta
tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat tggttgatta
tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag tatagaaccg
tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga ctatttgcaa
agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg gaagcatatt
tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca tgtatactaa
actcacaaat tagagcttca atttaattat atcagttatt actcgggcgt aatgattttt
ataatgacga aaaaaaaaaa attggaaaga aaaggggggg ggggcagcgt tgggtcctgg
ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc
tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga
gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc
agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc
ggtatcatta cccccatgaa cagaaattcc cccttacacg gaggcatcaa gtgaccaaac
aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg
agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc
acgctgatga gctttaccgc agccctcgag ggataagctt catttttaga taaaatttat
taatcatcat taatttcttg aaaaacattt tatttattga tcttttataa caaaaaaccc
ttctaaaagt ttatttttga atgaaaaact tataaaaatt tatgaaaact acaaaaaata
aaatttttaa ttaaaataat tttgataaga acttcaatct ttgactagct agcttagtca
tttttgagat ttaattaata ttttatgttt attcatatat aaactattca aaatattata
gaatttaaac attttaacat cttaatcatt cataaataac taaaaatcaa agtattacat
caataaataa cttttactca atgtcaaaga attattgggg ttggggttgg ggttggggtt
ggggttgggg ttggggttgg ggttggggtt ggggttgggg ttggggttgg ggttggggtt
ggggttgggg ttggggttgg ggttggggtt ggggttgggg ttggggttgg ggttggggtt
ggggttgggg ttggggttgg ggttggggtt ggggttgggg ttggggttgg ggttggggtt
ggggttgggg ttggggttgg ggttggggtt ggggttgggg ttggggttgg ggttggggtt
ggggtgggaa aacagcattc aggtattaga agaatatcct gattcaggtg aaaatattgt
tgatgcgcgg gatcctcggg gacaccaaat atggcgatct cggccttttc gtttcttgga
gctgggacat gtttgccatc gatccatcta ccaccagaac ggccgttaga tctgctgcca
ccgttgtttc caccgaagaa accaccgttg ccgtaaccac cacgacggtt gttgctaaag
aagctgccac cgccacggcc accgttgtag ccgccgttgt tgttattgta gttgctcatg
ttatttctgg cacttcttgg ttttcctctt aagtgaggag gaacataacc attctcgttg
ttgtcgttga tgcttaaatt ttgcacttgt tcgctcagtt cagccataat atgaaatgct
tttcttgttg ttcttacgga ataccacttg ccacctatca ccacaactaa ctttttcccg
ttcctccatc tcttttatat tttttttctc gatcgagttc aagagaaaaa aaaagaaaaa
gcaaaaagaa aaaaggaaag cgcgcctcgt tcagaatgac acgtatagaa tgatgcatta
ccttgtcatc ttcagtatca tactgttcgt atacatactt actgacattc ataggtatac
atatatacac atgtatatat atcgtatgct gcagctttaa ataatcggtg tcactacata
agaacacctt tggtggaggg aacatcgttg gtaccattgg gcgaggtggc ttctcttatg
gcaaccgcaa gagccttgaa cgcactctca ctacggtgat gatcattctt gcctcgcaga
caatcaacgt ggagggtaat tctgctagcc tctgcaaagc tttcaagaaa atgcgggatc
atctcgcaag agagatctcc tactttctcc ctttgcaaac caagttcgac aactgcgtac
ggcctgttcg aaagatctac caccgctctg gaaagtgcct catccaaagg cgcaaatcct
gatccaaacc tttttactcc acgcgccagt agggcctctt taaaagcttg accgagagca
atcccgcagt cttcagtggt gtgatggtcg tctatgtgta agtcaccaat gcactcaacg
attagcgacc agccggaatg cttggccaga gcatgtatca tatggtccag aaaccctata
cctgtgtgga cgttaatcac ttgcgattgt gtggcctgtt ctgctactgc ttctgcctct
ttttctggga agatcgagtg ctctatcgct aggggaccac cctttaaaga gatcgcaatc
tgaatcttgg tttcatttgt aatacgcttt actagggctt tctgctctgt catctttgcc
ttcgtttatc ttgcctgctc attttttagt atattcttcg aagaaatcac attactttat
ataatgtata attcattatg tgataatgcc aatcgctaag aaaaaaaaag agtcatccgc
taggtggaaa aaaaaaaatg aaaatcatta ccgaggcata aaaaaatata gagtgtacta
gaggaggcca agagtaatag aaaaagaaaa ttgcgggaaa ggactgtgtt atgacttccc
tgactaatgc cgtgttcaaa cgatacctgg cagtgactcc tagcgctcac caagctctta
aaacgagaat taagaaaaag tcgtcatctt tcgataagtt tttcccacag caaagcaata
gtagaaaaaa acaatgggaa acgttgaatg aagacaaagc gtcgtggttt aaaaggaaat
acgctcacgt acatgctagg gaacaggacc gtgcagcgga tcccgcgcat caacaatatt
ttcacctgaa tcaggatatt cttctaatac ctgaatgctg ttttcccacc ccaaccccaa
ccccaacccc aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa
ccccaacccc aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa
ccccaacccc aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa
ccccaacccc aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa
ccccaacccc aaccccaata attctttgac attgagtaaa agttatttat tgatgtaata
ctttgatttt tagttattta tgaatgatta agatgttaaa atgtttaaat tctataatat
tttgaatagt ttatatatga ataaacataa aatattaatt aaatctcaaa aatgactaag
ctagctagtc aaagattgaa gttcttatca aaattatttt aattaaaaat tttatttttt
gtagttttca taaattttta taagtttttc attcaaaaat aaacttttag aagggttttt
tgttataaaa gatcaataaa taaaatgttt ttcaagaaat taatgatgat taataaattt
tatctaaaaa tgaagcttat ccctcgaggg ctgcctcgcg cgtttcggtg atgacggtga
aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattaat tcggtcgaaa
aaagaaaagg agagggccaa gagggagggc attggtgact attgagcacg tgagtatacg
tgattaagca cacaaaggca gcttggagta tgtctgttat taatttcaca ggtagttctg
gtccattggt gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt gctctagatt
ccgatgctga cttgctgggt attatatgtg tgcccaatag aaagagaaca attgacccgg
ttattgcaag gaaaatttca agtcttgtaa aagcatataa aaatagttca ggcactccga
aatacttggt tggcgtgttt cgtaatcaac ctaaggagga tgttttggct ctggtcaatg
attacggcat tgatatcgtc caactgcatg gagatgagtc gtggcaagaa taccaagagt
tcctcggttt gccagttatt aaaagactcg tatttccaaa agactgcaac atactactca
gtgcagcttc acagaaacct cattcgttta ttcccttgtt tgattcagaa gcaggtggga
caggtgaact tttggattgg aactcgattt ctgactgggt tggaaggcaa gagagccccg
aaagcttaca ttttatgtta gctggtggac tgacgccaga aaatgttggt gatgcgctta
gattaaatgg cgttattggt gttgatgtaa gcggaggtgt ggagacaaat ggtgtaaaag
actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt actgagtagt
atttatttaa gtattgtttg tgcacttgcc tgcaggcctt ttgaaaagca agcataaaag
atctaaacat aaaatctgta aaataacaag atgtaaagat aatgctaaat catttggctt
tttgattgat tgtacaggaa aatatacatc gcagggggtt gacttttacc atttcaccgc
aatggaatca aacttgttga agagaatgtt cacaggcgca tacgctacaa tgacccgatt
cttgctagcc ttttctcggt cttgcaaaca accgccggca gcttagtata taaatacaca
tgtacatacc tctctccgta tcctcgtaat cattttcttg tatttatcgt cttttcgctg
taaaaacttt atcacactta tctcaaatac acttattaac cgcttttact attatcttct
acgctgacag taatatcaaa cagtgacaca tattaaacac agtggtttct ttgcataaac
accatcagcc tcaagtcgtc aagtaaagat ttcgtgttca tgcagataga taacaatcta
tatgttgata attagcgttg cctcatcaat gcgagatccg tttaaccgga ccctagtgca
cttaccccac gttcggtcca ctgtgtgccg aacatgctcc ttcactattt taacatgtgg
aattaattct aaatcctctt tatatgatct gccgatagat agttctaagt cattgaggtt
catcaacaat tggattttct gtttactcga cttcaggtaa atgaaatgag atgatacttg
cttatctcat agttaactct aagaggtgat acttatttac tgtaaaactg tgacgataaa
accggaagga agaataagaa aactcgaact gatctataat gcctattttc tgtaaagagt
ttaagctatg aaagcctcgg cattttggcc gctcctaggt agtgcttttt ttccaaggac
aaaacagttt ctttttcttg agcaggtttt atgtttcggt aatcataaac aataaataaa
ttatttcatt tatgtttaaa aataaaaaat aaaaaagtat tttaaatttt taaaaaagtt
gattataagc atgtgacctt ttgcaagcaa ttaaattttg caatttgtga ttttaggcaa
aagttacaat ttctggctcg tgtaatatat gtatgctaaa gtgaactttt acaaagtcga
tatggactta gtcaaaagaa attttcttaa aaatatatag cactagccaa tttagcactt
ctttatgaga tatattatag actttattaa gccagatttg tgtattatat gtatttaccc
ggcgaatcat ggacatacat tctgaaatag gtaatattct ctatggtgag acagcataga
taacctagga tacaagttaa aagctagtac tgttttgcag taattttttt cttttttata
agaatgttac cacctaaata agttataaag tcaatagtta agtttgatat ttgattgtaa
aataccgtaa tatatttgca tgatcaaaag gctcaatgtt gactagccag catgtcaacc
actatattga tcaccgatat atggacttcc acaccaacta gtaatatgac aataaattca
agatattctt catgagaatg gcccaga
//