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ID   PYESHISA   preliminary; circular DNA; SYN; 5930 BP.
XX
AC   IG1143;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pYESHisA - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pON3 from pBR322 & pON1
RC   pON5 from pON3 & lambda
RC   pON163 from pON5 & ARS1
RC   pDW222, pDW225 from HIS2 gene & URA gene
RC   pDW227 from pGEM-3 & pON163
RC   pDW229 from pGEM-3 & pON163
RC   pDW232 from pGEM-3 & pDW227 & pDW229
RA   Weilguny D., Praetorius M., Carr A.M., Egel R., Nielsen O.;
RT   "New vectors in fission yeast: application for cloning the his2
RT   gene";
RL   Gene 99:47-54(1991).
XX
CC   NM (pYESHisA)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (S.cerevisiae INVSc1)(S.cerevisiae INVSc2)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (2micron)
CC   BR (pYESHisB)(pYESHisC)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp
FT                   2. yeast, CYC1 gene
FT                   3. yeast, URA3 gene
FT                   3. yeast, GAL1 gene
FT                   5. yeast 2 micron, ori
FT                   6. histidine codons
FT                   -> pYESHisA 5930bp"
FT   terminator      0..0
FT                   /note="TER yeast CYC1 gene"
FT   misc_binding    0..0
FT                   /note="MCS XbaI-SphI-XhoI-NotI-BstXI-EcoRI-
FT                   BstXI-BamHI-SacI-KpnI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT enterokinase (EK)"
FT   repeat_unit     0..0
FT                   /note="RPT histidine codon (6)"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   promoter        complement(0..0)
FT                   /note="PRO yeast GAL1 gene"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   misc_binding    1706..1706
FT                   /note="SIT SnaBI"
FT   misc_binding    2936..2936
FT                   /note="SIT ClaI"
FT   misc_binding    2944..2944
FT                   /note="SIT NheI"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene (uracil)"
FT   misc_binding    3547..3547
FT                   /note="SIT ApaI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    4732..4732
FT                   /note="SIT BglI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 5930 BP; 1676 A; 1251 C; 1339 G; 1664 T; 0 other;
     ggccgcaaat taaagccttc gagcgtccca aaaccttctc aagcaaggtt ttcagtataa
     tgttacatgc gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat ataaataacg
     ttcttaatac taacataact ataaaaaaat aaatagggac ctagacttca ggttgtctaa
     ctccttcctt ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag cgtgacataa
     ctaattacat gatgcggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
     ctgcagaatt ccagcacact ggcggccgtt actagtggat cgatggggat ccgagctcgg
     taccaagctt gtcgaccacc gtccttgtcg tcgtcgtctg catgatgatg atgatgatga
     cccatggtag cttaatattc cctatagtga gtcgtattac agctgctagt agtccgatcc
     ggggtttttt ctccttgacg ttaaagtata gaggtatatt aacaattttt tgttgatact
     tttattacat ttgaataaga agtaatacaa accgaaaatg ttgaaagtat tagttaaagt
     ggttaatgca gtttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg
     attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa
     tttgattcgt tcatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata
     accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat
     ctgcgtttca ggaacgcgac cggtgaggac gaggacgcac ggaggagagt cttccttcgg
     agggctgtca cccgctcggc ggcttctaat ccgtactagt ggatcatccc cacgcgccct
     gtagcgcccc attaagcgcg gcgggtgtgg tggttacgcc cagcgtgacc cctacacttc
     ccaccgccct agcccccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     gctttccccg tcaagctcta aatgggggca tccgtttacc cttccgattt actgctttac
     ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     gatagaccct ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     tggaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     tgccgatttc gggctattcg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     ttaacaaaat attaacgttt acaatttaaa tatttgctta tacaatcttc ctgtttttgg
     ggcttttctg attatcaacc ggggtggagc ttcccattgc gaataccgct tccacaaaca
     ttgctcaaaa gtatctcttt gctatatatc tctgtgctat atccctatat aacctaccca
     tccacctttc gctccttgaa cttgcatcta aactcgacct ctacattttt tatgtttatc
     tctagtatta ctctttagac aaaaaaattg tagtaagaac tattcataga gtgaatcgaa
     aacaatacga aaatgtaaac atttcctata cgtagtatat agagacaaaa tagaagaaac
     cgttcataat tttctgacca atgaagaatc atcaacgcta tcactttctg ttcacaaagt
     atgcgcaatc cacatcggta tagaatataa tcggggatgc ctttatcttg aaaaaatgca
     cccgcagctt cgctagtaat cagtaaacgc gggaagtgga gtcaggcttt ttttatggaa
     gagaaaatag acaccaaagt agccttcttc taaccttaac ggacctacag tgcaaaaagt
     tatcaagaga ctgcattata gagcgcacaa aggagaaaaa aagtaatcta agatgctttg
     ttagaaaaat agcgctctcg ggatgcattt ttgtagaaca aaaaagaagt atagattctt
     tgttggtaaa atagcgctct cgcgttgcat ttctgttctg taaaaatgca gctcagattc
     tttgtttgaa aaattagcgc tctcgtcgcg ttgcattttt gttttacaaa aatgaagcac
     agattcttcg ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc
     tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa
     gcacagatgc ttcgttaaca aagatatgct attgaagtgc aagatggaaa cgcagaaaat
     gaaccgggga tgcgacgtgc aagattacct atgcaataga tgcaatagtt tctccaggaa
     ccgaaataca tacattgtct tccgtaaagc gctagactat atattattat acaggttcaa
     atatactatc tgtttcaggg aaaactccca ggttcggatg ttcaaaattc aatgatgggt
     aacaagtacg atcgtaaatc tgtaaaacag tttgtcggat attaggctgt atctcctcaa
     agcgtattcg aatatcattg agaagctgca gcgtcacatc ggataataat gatggcagcc
     attgtagaag tgccttttgc atttctagtc tctttctcgg tctagctagt tttactacat
     cgcgaagata gaatcttaga tcacactgcc tttgctgagc tggatcaata gagtaacaaa
     agagtggtaa ggcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac
     ggagtatcta gtatagtcta tagtccgtgg aattaattct catctttgac agcttatcat
     cgataagcta gcttttcaat tcaattcatc attttttttt tattcttttt tttgatttcg
     gtttctttga aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga
     gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc
     agtattctta acccaactgc acagaacaaa aacctgcagg aaacgaagat aaatcatgtc
     gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt
     taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa
     ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt
     ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc
     caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa
     attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca
     cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa
     ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg
     agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt
     tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc
     cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga
     tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg caaagggaag
     ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag
     atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca
     aattagagct tcaatttaat tatatcagtt attacccatt gaaaaaggaa gagtatgagt
     attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg atacactatt atcccgtatt
     gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     tgggaaccgg agctgaatga agccatacca aacgacgaga gtgacaccac gatgcctgta
     gcaatgccaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     caacaattaa tagactgaat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     atcattgcag cactggggcc agatggtaag cgctcccgta tcgtagttat ctacacgacg
     gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     agcaacgcgg cctttttacg gttcctgggc ttttgctggc cttttgctca catgttcttt
     cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
//