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ID   PYESHISB   preliminary; circular DNA; SYN; 5920 BP.
XX
AC   IG1144;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pYESHisB - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pON3 from pBR322 & pON1
RC   pON5 from pON3 & lambda
RC   pON163 from pON5 & ARS1
RC   pDW222, pDW225 from HIS2 gene & URA gene
RC   pDW227 from pGEM-3 & pON163
RC   pDW229 from pGEM-3 & pON163
RC   pDW232 from pGEM-3 & pDW227 & pDW229
RA   Weilguny D., Praetorius M., Carr A.M., Egel R., Nielsen O.;
RT   "New vectors in fission yeast: application for cloning the his2
RT   gene";
RL   Gene 99:47-54(1991).
XX
CC   NM (pYESHisB)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (S.cerevisiae INVSc1)(S.cerevisiae INVSc2)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (2micron)
CC   BR (pYESHisA)(pYESHisC)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp
FT                   2. yeast, CYC1 gene
FT                   3. yeast, URA3 gene
FT                   3. yeast, GAL1 gene
FT                   5. yeast 2 micron, ori
FT                   6. histidine codons
FT                   -> pYESHisB 5920bp"
FT   terminator      0..0
FT                   /note="TER yeast CYC1 gene"
FT   misc_binding    0..0
FT                   /note="MCS XbaI-SphI-XhoI-NotI-BstXI-EcoRI-
FT                   BstXI-BamHI-SacI-KpnI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT enterokinase (EK)"
FT   repeat_unit     0..0
FT                   /note="RPT histidine codon (6)"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   promoter        complement(0..0)
FT                   /note="PRO yeast GAL1 gene"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   misc_binding    1706..1706
FT                   /note="SIT SnaBI"
FT   misc_binding    2936..2936
FT                   /note="SIT ClaI"
FT   misc_binding    2944..2944
FT                   /note="SIT NheI"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene (uracil)"
FT   misc_binding    3547..3547
FT                   /note="SIT ApaI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    4732..4732
FT                   /note="SIT BglI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 5920 BP; 1674 A; 1250 C; 1334 G; 1662 T; 0 other;
     ggccgcaaat taaagccttc gagcgtccca aaaccttctc aagcaaggtt ttcagtataa
     tgttacatgc gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat ataaataacg
     ttcttaatac taacataact ataaaaaaat aaatagggac ctagacttca ggttgtctaa
     ctccttcctt ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag cgtgacataa
     ctaattacat gatgcggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
     ctgcagaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagctt
     gtcgaccacc gtccttgtcg tcgtcgtctg catgatgatg atgatgatga cccatggtag
     cttaatattc cctatagtga gtcgtattac agctgctagt agtccgatcc ggggtttttt
     ctccttgacg ttaaagtata gaggtatatt aacaattttt tgttgatact tttattacat
     ttgaataaga agtaatacaa accgaaaatg ttgaaagtat tagttaaagt ggttaatgca
     gtttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg attaattacc
     ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa tttgattcgt
     tcatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata accataaaag
     ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca
     ggaacgcgac cggtgaggac gaggacgcac ggaggagagt cttccttcgg agggctgtca
     cccgctcggc ggcttctaat ccgtactagt ggatcatccc cacgcgccct gtagcgcccc
     attaagcgcg gcgggtgtgg tggttacgcc cagcgtgacc cctacacttc ccaccgccct
     agcccccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     tcaagctcta aatgggggca tccgtttacc cttccgattt actgctttac ggcacctcga
     ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagaccct
     ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tggaaactgg
     aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     gggctattcg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     attaacgttt acaatttaaa tatttgctta tacaatcttc ctgtttttgg ggcttttctg
     attatcaacc ggggtggagc ttcccattgc gaataccgct tccacaaaca ttgctcaaaa
     gtatctcttt gctatatatc tctgtgctat atccctatat aacctaccca tccacctttc
     gctccttgaa cttgcatcta aactcgacct ctacattttt tatgtttatc tctagtatta
     ctctttagac aaaaaaattg tagtaagaac tattcataga gtgaatcgaa aacaatacga
     aaatgtaaac atttcctata cgtagtatat agagacaaaa tagaagaaac cgttcataat
     tttctgacca atgaagaatc atcaacgcta tcactttctg ttcacaaagt atgcgcaatc
     cacatcggta tagaatataa tcggggatgc ctttatcttg aaaaaatgca cccgcagctt
     cgctagtaat cagtaaacgc gggaagtgga gtcaggcttt ttttatggaa gagaaaatag
     acaccaaagt agccttcttc taaccttaac ggacctacag tgcaaaaagt tatcaagaga
     ctgcattata gagcgcacaa aggagaaaaa aagtaatcta agatgctttg ttagaaaaat
     agcgctctcg ggatgcattt ttgtagaaca aaaaagaagt atagattctt tgttggtaaa
     atagcgctct cgcgttgcat ttctgttctg taaaaatgca gctcagattc tttgtttgaa
     aaattagcgc tctcgtcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg
     ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt
     tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc
     ttcgttaaca aagatatgct attgaagtgc aagatggaaa cgcagaaaat gaaccgggga
     tgcgacgtgc aagattacct atgcaataga tgcaatagtt tctccaggaa ccgaaataca
     tacattgtct tccgtaaagc gctagactat atattattat acaggttcaa atatactatc
     tgtttcaggg aaaactccca ggttcggatg ttcaaaattc aatgatgggt aacaagtacg
     atcgtaaatc tgtaaaacag tttgtcggat attaggctgt atctcctcaa agcgtattcg
     aatatcattg agaagctgca gcgtcacatc ggataataat gatggcagcc attgtagaag
     tgccttttgc atttctagtc tctttctcgg tctagctagt tttactacat cgcgaagata
     gaatcttaga tcacactgcc tttgctgagc tggatcaata gagtaacaaa agagtggtaa
     ggcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac ggagtatcta
     gtatagtcta tagtccgtgg aattaattct catctttgac agcttatcat cgataagcta
     gcttttcaat tcaattcatc attttttttt tattcttttt tttgatttcg gtttctttga
     aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt
     agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta
     acccaactgc acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca
     tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg
     cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg
     gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg
     actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat
     tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac
     tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg
     ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga
     ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact
     aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa
     agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt
     ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct
     acaggatctg acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag
     gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag
     caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct
     tcaatttaat tatatcagtt attacccatt gaaaaaggaa gagtatgagt attcaacatt
     tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     tgatgagcac ttttaaagtt ctgctatgtg atacactatt atcccgtatt gacgccgggc
     aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     agctgaatga agccatacca aacgacgaga gtgacaccac gatgcctgta gcaatgccaa
     caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     tagactgaat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     cactggggcc agatggtaag cgctcccgta tcgtagttat ctacacgacg gggagtcagg
     caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     cctttttacg gttcctgggc ttttgctggc cttttgctca catgttcttt cctgcgttat
     cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     aaccgcctct ccccgcgcgt tggccgattc attaatgcag
//