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ID PYESHISB preliminary; circular DNA; SYN; 5920 BP.
XX
AC IG1144;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pYESHisB - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pON3 from pBR322 & pON1
RC pON5 from pON3 & lambda
RC pON163 from pON5 & ARS1
RC pDW222, pDW225 from HIS2 gene & URA gene
RC pDW227 from pGEM-3 & pON163
RC pDW229 from pGEM-3 & pON163
RC pDW232 from pGEM-3 & pDW227 & pDW229
RA Weilguny D., Praetorius M., Carr A.M., Egel R., Nielsen O.;
RT "New vectors in fission yeast: application for cloning the his2
RT gene";
RL Gene 99:47-54(1991).
XX
CC NM (pYESHisB)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Invitrogen)
CC HO (S.cerevisiae INVSc1)(S.cerevisiae INVSc2)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (2micron)
CC BR (pYESHisA)(pYESHisC)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC19 2686bp
FT 2. yeast, CYC1 gene
FT 3. yeast, URA3 gene
FT 3. yeast, GAL1 gene
FT 5. yeast 2 micron, ori
FT 6. histidine codons
FT -> pYESHisB 5920bp"
FT terminator 0..0
FT /note="TER yeast CYC1 gene"
FT misc_binding 0..0
FT /note="MCS XbaI-SphI-XhoI-NotI-BstXI-EcoRI-
FT BstXI-BamHI-SacI-KpnI-HindIII"
FT misc_binding 0..0
FT /note="SIT enterokinase (EK)"
FT repeat_unit 0..0
FT /note="RPT histidine codon (6)"
FT misc_feature 0..0
FT /note="ATG"
FT promoter complement(0..0)
FT /note="PRO bacteriophage T7"
FT promoter complement(0..0)
FT /note="PRO yeast GAL1 gene"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
FT rep_origin 0..0
FT /note="ORI yeast 2 micron"
FT misc_binding 1706..1706
FT /note="SIT SnaBI"
FT misc_binding 2936..2936
FT /note="SIT ClaI"
FT misc_binding 2944..2944
FT /note="SIT NheI"
FT CDS 0..0
FT /note="ANT yeast URA3 gene (uracil)"
FT misc_binding 3547..3547
FT /note="SIT ApaI"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 4732..4732
FT /note="SIT BglI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ Sequence 5920 BP; 1674 A; 1250 C; 1334 G; 1662 T; 0 other;
ggccgcaaat taaagccttc gagcgtccca aaaccttctc aagcaaggtt ttcagtataa
tgttacatgc gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat ataaataacg
ttcttaatac taacataact ataaaaaaat aaatagggac ctagacttca ggttgtctaa
ctccttcctt ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag cgtgacataa
ctaattacat gatgcggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
ctgcagaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagctt
gtcgaccacc gtccttgtcg tcgtcgtctg catgatgatg atgatgatga cccatggtag
cttaatattc cctatagtga gtcgtattac agctgctagt agtccgatcc ggggtttttt
ctccttgacg ttaaagtata gaggtatatt aacaattttt tgttgatact tttattacat
ttgaataaga agtaatacaa accgaaaatg ttgaaagtat tagttaaagt ggttaatgca
gtttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg attaattacc
ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa tttgattcgt
tcatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata accataaaag
ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca
ggaacgcgac cggtgaggac gaggacgcac ggaggagagt cttccttcgg agggctgtca
cccgctcggc ggcttctaat ccgtactagt ggatcatccc cacgcgccct gtagcgcccc
attaagcgcg gcgggtgtgg tggttacgcc cagcgtgacc cctacacttc ccaccgccct
agcccccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
tcaagctcta aatgggggca tccgtttacc cttccgattt actgctttac ggcacctcga
ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagaccct
ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tggaaactgg
aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
gggctattcg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
attaacgttt acaatttaaa tatttgctta tacaatcttc ctgtttttgg ggcttttctg
attatcaacc ggggtggagc ttcccattgc gaataccgct tccacaaaca ttgctcaaaa
gtatctcttt gctatatatc tctgtgctat atccctatat aacctaccca tccacctttc
gctccttgaa cttgcatcta aactcgacct ctacattttt tatgtttatc tctagtatta
ctctttagac aaaaaaattg tagtaagaac tattcataga gtgaatcgaa aacaatacga
aaatgtaaac atttcctata cgtagtatat agagacaaaa tagaagaaac cgttcataat
tttctgacca atgaagaatc atcaacgcta tcactttctg ttcacaaagt atgcgcaatc
cacatcggta tagaatataa tcggggatgc ctttatcttg aaaaaatgca cccgcagctt
cgctagtaat cagtaaacgc gggaagtgga gtcaggcttt ttttatggaa gagaaaatag
acaccaaagt agccttcttc taaccttaac ggacctacag tgcaaaaagt tatcaagaga
ctgcattata gagcgcacaa aggagaaaaa aagtaatcta agatgctttg ttagaaaaat
agcgctctcg ggatgcattt ttgtagaaca aaaaagaagt atagattctt tgttggtaaa
atagcgctct cgcgttgcat ttctgttctg taaaaatgca gctcagattc tttgtttgaa
aaattagcgc tctcgtcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg
ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt
tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc
ttcgttaaca aagatatgct attgaagtgc aagatggaaa cgcagaaaat gaaccgggga
tgcgacgtgc aagattacct atgcaataga tgcaatagtt tctccaggaa ccgaaataca
tacattgtct tccgtaaagc gctagactat atattattat acaggttcaa atatactatc
tgtttcaggg aaaactccca ggttcggatg ttcaaaattc aatgatgggt aacaagtacg
atcgtaaatc tgtaaaacag tttgtcggat attaggctgt atctcctcaa agcgtattcg
aatatcattg agaagctgca gcgtcacatc ggataataat gatggcagcc attgtagaag
tgccttttgc atttctagtc tctttctcgg tctagctagt tttactacat cgcgaagata
gaatcttaga tcacactgcc tttgctgagc tggatcaata gagtaacaaa agagtggtaa
ggcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac ggagtatcta
gtatagtcta tagtccgtgg aattaattct catctttgac agcttatcat cgataagcta
gcttttcaat tcaattcatc attttttttt tattcttttt tttgatttcg gtttctttga
aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt
agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta
acccaactgc acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca
tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg
cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg
gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg
actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat
tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac
tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg
ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga
ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact
aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa
agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt
ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct
acaggatctg acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag
gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag
caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct
tcaatttaat tatatcagtt attacccatt gaaaaaggaa gagtatgagt attcaacatt
tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
tgatgagcac ttttaaagtt ctgctatgtg atacactatt atcccgtatt gacgccgggc
aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
agctgaatga agccatacca aacgacgaga gtgacaccac gatgcctgta gcaatgccaa
caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
tagactgaat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
cactggggcc agatggtaag cgctcccgta tcgtagttat ctacacgacg gggagtcagg
caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
cctttttacg gttcctgggc ttttgctggc cttttgctca catgttcttt cctgcgttat
cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
aaccgcctct ccccgcgcgt tggccgattc attaatgcag
//