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ID PYESHISC preliminary; circular DNA; SYN; 5928 BP.
XX
AC IG1145;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pYESHisC - complete.
XX
KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pON3 from pBR322 & pON1
RC pON5 from pON3 & lambda
RC pON163 from pON5 & ARS1
RC pDW222, pDW225 from HIS2 gene & URA gene
RC pDW227 from pGEM-3 & pON163
RC pDW229 from pGEM-3 & pON163
RC pDW232 from pGEM-3 & pDW227 & pDW229
RA Weilguny D., Praetorius M., Carr A.M., Egel R., Nielsen O.;
RT "New vectors in fission yeast: application for cloning the his2
RT gene";
RL Gene 99:47-54(1991).
XX
CC NM (pYESHisC)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Invitrogen)
CC HO (S.cerevisiae INVSc1)(S.cerevisiae INVSc2)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (2micron)
CC BR (pYESHisA)(pYESHisB)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC19 2686bp
FT 2. yeast, CYC1 gene
FT 3. yeast, URA3 gene
FT 3. yeast, GAL1 gene
FT 5. yeast 2 micron, ori
FT 6. histidine codons
FT -> pYESHisC 5928bp"
FT terminator 0..0
FT /note="TER yeast CYC1 gene"
FT misc_binding 0..0
FT /note="MCS XbaI-SphI-XhoI-NotI-BstXI-EcoRI-
FT BstXI-BamHI-SacI-KpnI-HindIII"
FT misc_binding 0..0
FT /note="SIT enterokinase (EK)"
FT repeat_unit 0..0
FT /note="RPT histidine codon (6)"
FT misc_feature 0..0
FT /note="ATG"
FT promoter complement(0..0)
FT /note="PRO bacteriophage T7"
FT promoter complement(0..0)
FT /note="PRO yeast GAL1 gene"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
FT rep_origin 0..0
FT /note="ORI yeast 2 micron"
FT misc_binding 1706..1706
FT /note="SIT SnaBI"
FT misc_binding 2936..2936
FT /note="SIT ClaI"
FT misc_binding 2944..2944
FT /note="SIT NheI"
FT CDS 0..0
FT /note="ANT yeast URA3 gene (uracil)"
FT misc_binding 3547..3547
FT /note="SIT ApaI"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 4732..4732
FT /note="SIT BglI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ Sequence 5928 BP; 1676 A; 1252 C; 1336 G; 1664 T; 0 other;
ggccgcaaat taaagccttc gagcgtccca aaaccttctc aagcaaggtt ttcagtataa
tgttacatgc gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat ataaataacg
ttcttaatac taacataact ataaaaaaat aaatagggac ctagacttca ggttgtctaa
ctccttcctt ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag cgtgacataa
ctaattacat gatgcggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
ctgcagaatt ccagcacact ggcggccgtt actagtggat cgatggatcc gacctcggta
ccaagcttgt cgaccaccgt ccttgtcgtc gtcgtctgca tgatgatgat gatgatgacc
catggtagct taatattccc tatagtgagt cgtattacag ctgctagtag tccgatccgg
ggttttttct ccttgacgtt aaagtataga ggtatattaa caattttttg ttgatacttt
tattacattt gaataagaag taatacaaac cgaaaatgtt gaaagtatta gttaaagtgg
ttaatgcagt ttttgcattt atatatctgt taatagatca aaaatcatcg cttcgctgat
taattacccc agaaataagg ctaaaaaact aatcgcatta tcatcctatg gttgttaatt
tgattcgttc atttgaaggt ttgtggggcc aggttactgc caatttttcc tcttcataac
cataaaagct agtattgtag aatctttatt gttcggagca gtgcggcgcg aggcacatct
gcgtttcagg aacgcgaccg gtgaggacga ggacgcacgg aggagagtct tccttcggag
ggctgtcacc cgctcggcgg cttctaatcc gtactagtgg atcatcccca cgcgccctgt
agcgccccat taagcgcggc gggtgtggtg gttacgccca gcgtgacccc tacacttccc
accgccctag cccccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
tttccccgtc aagctctaaa tgggggcatc cgtttaccct tccgatttac tgctttacgg
cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
tagacccttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttg
gaaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
ccgatttcgg gctattcgtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
aacaaaatat taacgtttac aatttaaata tttgcttata caatcttcct gtttttgggg
cttttctgat tatcaaccgg ggtggagctt cccattgcga ataccgcttc cacaaacatt
gctcaaaagt atctctttgc tatatatctc tgtgctatat ccctatataa cctacccatc
cacctttcgc tccttgaact tgcatctaaa ctcgacctct acatttttta tgtttatctc
tagtattact ctttagacaa aaaaattgta gtaagaacta ttcatagagt gaatcgaaaa
caatacgaaa atgtaaacat ttcctatacg tagtatatag agacaaaata gaagaaaccg
ttcataattt tctgaccaat gaagaatcat caacgctatc actttctgtt cacaaagtat
gcgcaatcca catcggtata gaatataatc ggggatgcct ttatcttgaa aaaatgcacc
cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt caggcttttt ttatggaaga
gaaaatagac accaaagtag ccttcttcta accttaacgg acctacagtg caaaaagtta
tcaagagact gcattataga gcgcacaaag gagaaaaaaa gtaatctaag atgctttgtt
agaaaaatag cgctctcggg atgcattttt gtagaacaaa aaagaagtat agattctttg
ttggtaaaat agcgctctcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt
tgtttgaaaa attagcgctc tcgtcgcgtt gcatttttgt tttacaaaaa tgaagcacag
attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc
agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc
acagatgctt cgttaacaaa gatatgctat tgaagtgcaa gatggaaacg cagaaaatga
accggggatg cgacgtgcaa gattacctat gcaatagatg caatagtttc tccaggaacc
gaaatacata cattgtcttc cgtaaagcgc tagactatat attattatac aggttcaaat
atactatctg tttcagggaa aactcccagg ttcggatgtt caaaattcaa tgatgggtaa
caagtacgat cgtaaatctg taaaacagtt tgtcggatat taggctgtat ctcctcaaag
cgtattcgaa tatcattgag aagctgcagc gtcacatcgg ataataatga tggcagccat
tgtagaagtg ccttttgcat ttctagtctc tttctcggtc tagctagttt tactacatcg
cgaagataga atcttagatc acactgcctt tgctgagctg gatcaataga gtaacaaaag
agtggtaagg cctcgttaaa ggacaaggac ctgagcggaa gtgtatcgta cagtagacgg
agtatctagt atagtctata gtccgtggaa ttaattctca tctttgacag cttatcatcg
ataagctagc ttttcaattc aattcatcat ttttttttta ttcttttttt tgatttcggt
ttctttgaaa tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc
acagacttag attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag
tattcttaac ccaactgcac agaacaaaaa cctgcaggaa acgaagataa atcatgtcga
aagctacata taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta
atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg
aattactgga gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg
atatcttgac tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca
agtacaattt tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat
tgcagtactc tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg
gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg
aacctagagg ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag
aatatactaa gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta
ttgctcaaag agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg
gtgtgggttt agatgacaag ggagacgcat tgggtcaaca gtatagaacc gtggatgatg
tggtctctac aggatctgac attattattg ttggaagagg actatttgca aagggaaggg
atgctaaggt agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat
gcggccagca aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa
ttagagcttc aatttaatta tatcagttat tacccattga aaaaggaaga gtatgagtat
tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
ttttccaatg atgagcactt ttaaagttct gctatgtgat acactattat cccgtattga
cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
ggaaccggag ctgaatgaag ccataccaaa cgacgagagt gacaccacga tgcctgtagc
aatgccaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
acaattaata gactgaatgg aggcggataa agttgcagga ccacttctgc gctcggccct
tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
cattgcagca ctggggccag atggtaagcg ctcccgtatc gtagttatct acacgacggg
gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
caacgcggcc tttttacggt tcctgggctt ttgctggcct tttgctcaca tgttctttcc
tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcag
//