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ID   PYESHISC   preliminary; circular DNA; SYN; 5928 BP.
XX
AC   IG1145;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pYESHisC - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pON3 from pBR322 & pON1
RC   pON5 from pON3 & lambda
RC   pON163 from pON5 & ARS1
RC   pDW222, pDW225 from HIS2 gene & URA gene
RC   pDW227 from pGEM-3 & pON163
RC   pDW229 from pGEM-3 & pON163
RC   pDW232 from pGEM-3 & pDW227 & pDW229
RA   Weilguny D., Praetorius M., Carr A.M., Egel R., Nielsen O.;
RT   "New vectors in fission yeast: application for cloning the his2
RT   gene";
RL   Gene 99:47-54(1991).
XX
CC   NM (pYESHisC)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (S.cerevisiae INVSc1)(S.cerevisiae INVSc2)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (2micron)
CC   BR (pYESHisA)(pYESHisB)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp
FT                   2. yeast, CYC1 gene
FT                   3. yeast, URA3 gene
FT                   3. yeast, GAL1 gene
FT                   5. yeast 2 micron, ori
FT                   6. histidine codons
FT                   -> pYESHisC 5928bp"
FT   terminator      0..0
FT                   /note="TER yeast CYC1 gene"
FT   misc_binding    0..0
FT                   /note="MCS XbaI-SphI-XhoI-NotI-BstXI-EcoRI-
FT                   BstXI-BamHI-SacI-KpnI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT enterokinase (EK)"
FT   repeat_unit     0..0
FT                   /note="RPT histidine codon (6)"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   promoter        complement(0..0)
FT                   /note="PRO yeast GAL1 gene"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   misc_binding    1706..1706
FT                   /note="SIT SnaBI"
FT   misc_binding    2936..2936
FT                   /note="SIT ClaI"
FT   misc_binding    2944..2944
FT                   /note="SIT NheI"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene (uracil)"
FT   misc_binding    3547..3547
FT                   /note="SIT ApaI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    4732..4732
FT                   /note="SIT BglI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 5928 BP; 1676 A; 1252 C; 1336 G; 1664 T; 0 other;
     ggccgcaaat taaagccttc gagcgtccca aaaccttctc aagcaaggtt ttcagtataa
     tgttacatgc gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat ataaataacg
     ttcttaatac taacataact ataaaaaaat aaatagggac ctagacttca ggttgtctaa
     ctccttcctt ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag cgtgacataa
     ctaattacat gatgcggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
     ctgcagaatt ccagcacact ggcggccgtt actagtggat cgatggatcc gacctcggta
     ccaagcttgt cgaccaccgt ccttgtcgtc gtcgtctgca tgatgatgat gatgatgacc
     catggtagct taatattccc tatagtgagt cgtattacag ctgctagtag tccgatccgg
     ggttttttct ccttgacgtt aaagtataga ggtatattaa caattttttg ttgatacttt
     tattacattt gaataagaag taatacaaac cgaaaatgtt gaaagtatta gttaaagtgg
     ttaatgcagt ttttgcattt atatatctgt taatagatca aaaatcatcg cttcgctgat
     taattacccc agaaataagg ctaaaaaact aatcgcatta tcatcctatg gttgttaatt
     tgattcgttc atttgaaggt ttgtggggcc aggttactgc caatttttcc tcttcataac
     cataaaagct agtattgtag aatctttatt gttcggagca gtgcggcgcg aggcacatct
     gcgtttcagg aacgcgaccg gtgaggacga ggacgcacgg aggagagtct tccttcggag
     ggctgtcacc cgctcggcgg cttctaatcc gtactagtgg atcatcccca cgcgccctgt
     agcgccccat taagcgcggc gggtgtggtg gttacgccca gcgtgacccc tacacttccc
     accgccctag cccccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     tttccccgtc aagctctaaa tgggggcatc cgtttaccct tccgatttac tgctttacgg
     cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     tagacccttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttg
     gaaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     ccgatttcgg gctattcgtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     aacaaaatat taacgtttac aatttaaata tttgcttata caatcttcct gtttttgggg
     cttttctgat tatcaaccgg ggtggagctt cccattgcga ataccgcttc cacaaacatt
     gctcaaaagt atctctttgc tatatatctc tgtgctatat ccctatataa cctacccatc
     cacctttcgc tccttgaact tgcatctaaa ctcgacctct acatttttta tgtttatctc
     tagtattact ctttagacaa aaaaattgta gtaagaacta ttcatagagt gaatcgaaaa
     caatacgaaa atgtaaacat ttcctatacg tagtatatag agacaaaata gaagaaaccg
     ttcataattt tctgaccaat gaagaatcat caacgctatc actttctgtt cacaaagtat
     gcgcaatcca catcggtata gaatataatc ggggatgcct ttatcttgaa aaaatgcacc
     cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt caggcttttt ttatggaaga
     gaaaatagac accaaagtag ccttcttcta accttaacgg acctacagtg caaaaagtta
     tcaagagact gcattataga gcgcacaaag gagaaaaaaa gtaatctaag atgctttgtt
     agaaaaatag cgctctcggg atgcattttt gtagaacaaa aaagaagtat agattctttg
     ttggtaaaat agcgctctcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt
     tgtttgaaaa attagcgctc tcgtcgcgtt gcatttttgt tttacaaaaa tgaagcacag
     attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc
     agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc
     acagatgctt cgttaacaaa gatatgctat tgaagtgcaa gatggaaacg cagaaaatga
     accggggatg cgacgtgcaa gattacctat gcaatagatg caatagtttc tccaggaacc
     gaaatacata cattgtcttc cgtaaagcgc tagactatat attattatac aggttcaaat
     atactatctg tttcagggaa aactcccagg ttcggatgtt caaaattcaa tgatgggtaa
     caagtacgat cgtaaatctg taaaacagtt tgtcggatat taggctgtat ctcctcaaag
     cgtattcgaa tatcattgag aagctgcagc gtcacatcgg ataataatga tggcagccat
     tgtagaagtg ccttttgcat ttctagtctc tttctcggtc tagctagttt tactacatcg
     cgaagataga atcttagatc acactgcctt tgctgagctg gatcaataga gtaacaaaag
     agtggtaagg cctcgttaaa ggacaaggac ctgagcggaa gtgtatcgta cagtagacgg
     agtatctagt atagtctata gtccgtggaa ttaattctca tctttgacag cttatcatcg
     ataagctagc ttttcaattc aattcatcat ttttttttta ttcttttttt tgatttcggt
     ttctttgaaa tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc
     acagacttag attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag
     tattcttaac ccaactgcac agaacaaaaa cctgcaggaa acgaagataa atcatgtcga
     aagctacata taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta
     atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg
     aattactgga gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg
     atatcttgac tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca
     agtacaattt tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat
     tgcagtactc tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg
     gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg
     aacctagagg ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag
     aatatactaa gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta
     ttgctcaaag agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg
     gtgtgggttt agatgacaag ggagacgcat tgggtcaaca gtatagaacc gtggatgatg
     tggtctctac aggatctgac attattattg ttggaagagg actatttgca aagggaaggg
     atgctaaggt agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat
     gcggccagca aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa
     ttagagcttc aatttaatta tatcagttat tacccattga aaaaggaaga gtatgagtat
     tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     ttttccaatg atgagcactt ttaaagttct gctatgtgat acactattat cccgtattga
     cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     ggaaccggag ctgaatgaag ccataccaaa cgacgagagt gacaccacga tgcctgtagc
     aatgccaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     acaattaata gactgaatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     cattgcagca ctggggccag atggtaagcg ctcccgtatc gtagttatct acacgacggg
     gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     caacgcggcc tttttacggt tcctgggctt ttgctggcct tttgctcaca tgttctttcc
     tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcag
//