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ID   PYEURA3    preliminary; circular DNA; SYN; 6170 BP.
XX
AC   U02457; IG1148;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pYEUra3 - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pYEUra3
RA   Kitts P.A.;
RT   "CLONTECH Vectors On Disc version 1.1";
RL   Unpublished (1993).
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RN   [2]
RP   1-6170
RC   pYEUra3
RA   Kitts P.A.;
RT   ;
RL   Submitted (07-OCT-1993) by:
RL   Kitts P.A., CLONTECH Laboratories, Inc.,
RL   4030 Fabian Way, Palo Alto, CA 94303, USA.
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CC   This sequence has been compiled from information in the sequence
CC   databases, published literature and other sources, together with
CC   partial sequences obtained by CLONTECH; this vector has not been
CC   completely sequenced. If you suspect there is an error in this
CC   sequence, please contact CLONTECH's Technical Service Department at
CC   (415) 424-8222 or (800) 662-2566, extension 3 or E-mail
CC   CLONTECH@BIOTECHNET.COM.
CC   NCBI gi: 413823
CC   NM (pYEUra3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)(yeast)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. yeast, CEN4
FT                   3. yeast, ARS1
FT                   4. yeast, URA3 gene
FT                   5. yeast, GAL1-GAL10 promoter
FT                   6. T7, promoter
FT                   7. f1, ori
FT                   -> pYEUra3 6170bp"
FT   misc_binding    0..0
FT                   /note="SIT NoeI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN4"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   misc_binding    2600..2600
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene (uracil)"
FT   misc_binding    150..1500
FT                   /note="SIT EcoRI"
FT   promoter        complement(0..0)
FT                   /note="PRO yeast GAL10 gene"
FT   promoter        0..0
FT                   /note="PRO yeast GAL1 gene"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-XbaI-SalI-XhoI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1"
XX
SQ   Sequence 6170 BP; 1719 A; 1331 C; 1329 G; 1791 T; 0 other;
     gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt
     gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg ccccccctcg
     aggtcgactc tagaggatcc ccgggtaccg agctcgaatt aattcggcta gaggtttttt
     ctccttgacg ttaaagtata gaggtatatt aacaattttt tgttgatact tttattacat
     ttgaataaga agtaatacaa accgaaaatg ttgaaagtat tagttaaagt ggttatgcag
     tttttgcatt tatatatctg ttaatagatc aaaaatcatc gcttcgctga ttaattaccc
     cagaaataag gctaaaaaac taatcgcatt atcatcctat ggttgttaat ttgattcgtt
     catttgaagg tttgtggggc caggttactg ccaatttttc ctcttcataa ccataaaagc
     tagtattgta gaatctttat tgttcggagc agtgcggcgc gaggcacatc tgcgtttcag
     gaacgcgacc ggtgaagacg aggacgcacg gaggagagtc ttccttcgga gggctgtcac
     ccgctcggcg gcttctaatc cgtacttcaa tatagcaatg agcagttaag cgtattactg
     aaagttccaa agagaaggtt tttttaggct aagataatgg ggctctttac atttccacaa
     catataagta agattagata tggatatgta tatggatatg tatatggtgg taatgccatg
     taatatgatt attaaacttc tttgcgtcca tccaaaaaaa aagtaagaat ttttgaaaat
     tcaatataaa tgacagctca gttacaaagt gaaagtactt ctaaaattgt tttggttaca
     ggtggtgctg gatacattgg ttcacacact gtggtagagc taattgagaa tggatatgac
     tgtgttgttg ctgataacct gtcgaattcg agctcggtac ccgggtaata actgatataa
     ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt
     ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc
     accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc
     agatcctgta gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt
     gtcatctaaa cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc
     catgtctctt tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac
     agtaccctta gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat
     caaaaggcct ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat
     acctgggccc accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac
     acccgcagag tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa
     gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga
     aaaatcagtc aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc
     aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg
     cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg
     ttccttatat gtagctttcg acatgattta tcttcgtttc ctgcaggttt ttgttctgtg
     cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata
     taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc
     aaaaaaattt caaggaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa
     aagcttacat tttatgttag ctggtggact gacgccagaa aatgttggtg atgcgcttag
     attaaatggc gttattggtg ttgatgtaag cggaggtgtg gagacaaatg gtgtaaaaga
     ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta ctgagtagta
     tttatttaag tattgtttgt gcacttgcct gcaggccttt tgaaaagcaa gcataaaaga
     tctaaacata aaatctgtaa aataacaaga tgtaaagata atgctaaatc atttggcttt
     ttgattgatt gtacaggaaa atatacatcg cagggggttg acttttacca tttcaccgca
     atggaatcaa acttgttgaa gagaatgttc acaggcgcat acgctacaat gacccgattc
     ttgctagcct tttctcggtc ttgcaaacaa ccgccaactc taagaggtga tacttattta
     ctgtaaaact gtgacgataa aaccggaagg aagaataaga aaactcgaac tgatctataa
     tgcctatttt ctgtaaagag tttaagctat gaaagcctcg gcattttggc cgctcctagg
     tagtgctttt tttccaagga caaaacagtt tctttttctt gagcaggttt tatgtttcgg
     taatcataaa caataaataa attatttcat ttatgtttaa aaataaaaaa taaaaaagta
     ttttaaattt ttaaaaaagt tgattataag catgtgacct tttgcaagca attaaatttt
     gcaatttgtg attttaggca aaagttacaa tttctggctc gtgtaatata tgtatgctaa
     agtgaacttt tacaaagtcg atatggactt agtcaaaaga aattttctta aaaatatata
     gcactagcca atttagcact tctttatgag atatattata gactttatta agccagattt
     gtgtattata tgtatttacc cggcgaatca tggacataca ttctgaaata ggtaatattc
     tctatggtga gacagcatag ataacctagg atacaagtta aaagctagta ctgttttgca
     gtaatttttt tcttttttat aagaatgtta ccacctaaat aagttataaa gtcaatagtt
     aagtttgata tttgattgta aaataccgta atatatttgc atgatcaaaa ggctcaatgt
     tgactagcca gcatgtcaac cactatattg atcaccgata tatggacttc cacaccaact
     agtaatatga caataaattc aagatattct tcatgagaat ggcccagctg cctcgcgcgt
     ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
//