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ID   PZ1918     preliminary; circular DNA; SYN; 5932 BP.
XX
AC   U03993;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pZ1918 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-5932
RC   pUC1918 from pUC18 & pUC19
RC   plasmid from pACYC184 & M13mVDX18
RC   pX1918 from plasmid & pUC1918
RC   pZ1918 from pUC1918 & pTZ1
RA   Schweizer H.P.;
RT   "Two plasmids, X1918 and Z1918, for easy recovery of the xylE and
RT   lacZ reporter genes";
RL   Gene 134:89-91(1993).
XX
RN   [2]
RP   450-532
RC   pRS414, pRS591 from pBR322 & pMC1403
RC   pRS415, pRS528 from pBR322 & pNK678
RC   pRS551, pRS550 from pRS415 & Tn903, kan gene
RC   pRS552, pRS577 from pRS414 & Tn903, kan gene
RC   pRS308 from pNK678 & pRS415
RC   pRS419 from pRS415
RC   pRS599 from pRS528 & IS10R-pIN promoter
RC   pRS475 from pRS415 & lacUV5 promoter
RC   pRS476 from pRS414 & lacUV5 promoter
RC   pRS575 from pRS552 & IS10R-pIN promoter
RC   lambda RS45 from pRS308 & lambda RP167
RC   [lambda NK610 from lambda]
RC   lambda RS88 from pRS308 & lambda RP167 & lambda NK610
RC   lambda RS74 from lambda RS45 & pRS475
RC   lambda RS91 from lambda RS88 & pRS475
RC   lambda RS72 from lambda RS45 & pRS415
RC   lambda RS242 from lambda RS45 & pRS599
RC   lambda RS74 from lambda RS45 & pRS475
RC   lambda RS194 from lambda RS45 & pRS552
RC   lambda RS198 from lambda RS45 & pRS575
RA   Simons R.W., Houman F., Kleckner N.;
RT   "Improved single and multicopy lac-based cloning vectors for protein
RT   and operon fusions";
RL   Gene 53:85-96(1987).
XX
RN   [3]
RP   1-5932
RC   pZ1918
RA   Schweizer H.P.;
RT   ;
RL   Submitted (17-DEC-1993) by:
RL   Schweizer H.P., Microbiology & Infectious Diseases, Univ. of Calgary,
RL   3330 Hospital Drive NW, Calgary Alberta T2N 4N1, CANADA.
XX
RN   [4]
RC   plasmid from pcos1EMBL & pTS71, xylE gene
RC   plasmid2 from M13mp18 & plasmid
RC   plasmid3 from plasmid2
RC   plasmid4 from plasmid3 & linker
RC   M13mXw5 from M13mp18 & plasmid4
RC   M13mVDX18 from M13mp18 & M13mXw5 & linker
RC   M13mMK010 from M13mTM010 & M13mXw5 & linker
RC   pVDX18 from M13mp18 & pKT240 & M13mVDX18, xylE gene
RC   pPAOM3 from pVDX18 & P.aeruginosa PAO algD gene promoter
RC   pPAONM3 from pPAOM3
RC   pCF21, pCF31 from pVDX18 & P.aeruginosa PAO algD gene promoter
RC   pNsiEV from pVDX18 & P.aeruginosa PAO algD gene promoter
RA   Konyecsni W.M., Deretic V.;
RT   "Broad-host-range plasmid and M13 bacteriophage-derived vectors
RT   for promoter analysis in Escherichia coli and Pseudomonas aeruginosa";
RL   Gene 74:375-386(1988).
XX
RN   [5]
RC   pNK591, pNK688 from pNK82 & linker
RC   pNK486, pNK663 from pNK82
RC   pNK744 from pNK486 & linker
RC   pNK743 from pNK663 & linker
RC   pNK745 from pNK579 & linker
RC   pNK816 from pNK series
RC   pNK897 from pNK816 & pNK743
RC   pNK898 from pNK816 & pNK744
RC   pNK678 from pMC1871 & pMC1403
RC   pNK736, pNK772, pNK774, pNK771, pNK733, pNK754 from pNK678
RC   pNK756, pNK859, pNK857, pNK732, pNK739 from pNK678
RC   NOTE: pIN = IS10 tnp gene promoter
RA   Simons R.W., Hoopes B.C., McClure W.R., Kleckner N.;
RT   "Three promoters near the termini of IS10: pIN, pOUT and pIII";
RL   Cell 34:673-682(1983).
XX
RN   [6]
RC   pMR1 from pKB280 & pMC7
RC   lambda h80 from lambda
RC   lambda 112 from lambda W205 & pKB155 & lambda RP167
RA   Maurer R., Meyer B.J., Ptashne M.;
RT   "Gene regulation at the right operator (OR) of bacteriophage
RT   lambda, I. OR3 and autogenous negative control by repressor";
RL   J. Mol. Biol. 139:147-161(1980).
XX
RN   [7]
RC   M13mTM010 from M13
RA   Misra T.K.;
RT   "DNA sequencing: a new strategy to create ordered deletions,
RT   modified M13 vector and improved reaction conditions for sequencing
RT   by dideoxy chain termination method";
RL   Meth. Enzymol. 155:119-138(1987).
XX
RN   [8]
RC   pTS71 from ColE1 & xylE gene
RA   Inouye S., Nakazawa A., Nakasawa T.;
RT   "Nucleotide sequence of the promoter region of the xylDEGF operon
RT   on TOL plasmid of Pseudomonas putida";
RL   Gene 29:323-330(1984).
XX
RN   [9]
RC   lambda RP167 from lambda
RA   Pastrana R.;
RT   ;
RL   Unpublished (1980).
XX
RN   [10]
RC   lambda W205 from lambda
RA   Barnes W.M., Siegel R.B., Reznikoff W.S.;
RT   "The construction of lambda transducing phages containing
RT   deletions defining regulatory elements of the lac and trp operons
RT   in E. coli";
RL   Mol. Gen. Genet. 129:201-215(1974).
XX
RN   [11]
RC   pTP12 from pBR322 & phage P22, amp gene
RA   Poteete A.;
RT   ;
RL   Unpublished (1980).
XX
CC   NCBI gi: 434652
CC   NM (pZ1918)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 NdeI-EcoRI 266bp 185..451,
FT                   \ MCS/lacZ [264bp]
FT                   2. pUC19 remove NdeI-EcoRI 212bp 185..397,
FT                   \ MCS/lacZ/2474bp
FT                   -> pUC1918 2740bp
FT                   1. pUC1918 EcoRI 2740bp 451..451
FT                   T4 DNA polymerase
FT                   2. pTZ1 BamHI-BamHI 3202bp
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pZ1918 5932bp [BamHI, no EcoRI]"
FT   RBS             539..542
FT                   /note="RBS E. coli lacZ gene"
FT   RBS             5738..5741
FT                   /note="RBS E. coli bla gene"
FT   misc_binding    399..455
FT                   /note="MCS HindIII-SphI-PstI-HincII-AccI-SalI-XbaI-
FT                   BamHI-XmaI-SmaI-KpnI-SacI-EcoRI-SacI-KpnI-SmaI-XmaI-
FT                   BamHI-XbaI-SalI-AccI-HincII-PstI-SphI-HindIII"
FT   misc_feature    458..532
FT                   /note="Escherichia coli trp operon DNA [3]"
FT   misc_feature    533..3639
FT                   /note="Escherichia coli lac operon DNA"
FT   misc_binding    3642..3698
FT                   /note="MCS"
FT   misc_feature    3619..3647
FT                   /note="lac operon DNA/polylinker junction [4]"
FT   misc_feature    450..3647
FT                   /note="fragment from pTZ1 [2]"
FT   misc_feature    1..449
FT                   /note="from pUC1918"
FT   misc_feature    3648..5932
FT                   /note="from pUC1918"
FT   misc_feature    450..3647
FT                   /note="from pTZ1"
FT   CDS             550..3624
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   NCBI gi: 434653"
FT   CDS             complement(4872..5732)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp);
FT                   NCBI gi: 434654"
XX
SQ   Sequence 5932 BP; 1385 A; 1568 C; 1621 G; 1358 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
     actctagagg atccccgggt accgagctcg gatccggaca accgatgaaa gcggcgacgc
     gcagttaatc ccacagccgc cagttccgct ggcggcattt taactttctt tatcacacag
     gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
     gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc
     gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat
     gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt tgttcccacg
     gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa
     ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg
     cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt
     ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gttggagtga cggcagttat
     ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat
     aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc
     cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta
     acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt
     gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa
     aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac
     accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg
     attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt
     cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc
     ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg
     ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa
     acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg
     agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg
     tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa
     tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc
     gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg
     aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc
     gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt
     cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt
     aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg
     aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg
     ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc
     gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg
     gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt
     aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc
     acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc
     tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc
     atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg
     caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg
     ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt
     gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac
     caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt
     acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac
     cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca
     ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac
     tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac
     cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt
     ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag
     ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg
     cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac
     gactcctgga gcccgtcagt atcggcggaa ttccagctga gcgccggtcg ctaccattac
     cagttggtct ggtgtcaaaa ataataataa ccgggcaggg gggatccgag ctcggtaccc
     ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg
     tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//