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ID R199 preliminary; DNA; SYN; 6411 BP.
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AC ATCC37140;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phage vector f1 R199 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC R199 from phage f1 & pBR322
RC R208 from R199 & pBR322
RC R209 from phage f1
RC R240 from phage f1
RA Boeke J.D., Vovis G.F., Zinder N.D.;
RT "Insertion mutant of bacteriophage f1 sensitive to EcoRI";
RL Proc. Natl. Acad. Sci. U.S.A. 76:2699-2702(1979).
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CC Created by Moore, July 1995, under contract with NCBI.
CC A filamentous phage DNA vector. (ATCC staff)
CC Medium is 1592 SM buffer.
CC NM (R199)
CC CM (yes)
CC NA (filamentous ss-DNA)
CC TP (linear)
CC ST ()
CC TY (phage)
CC SP (ATCC)
CC HO (bacteria-free lysate)(E.coli K38)(E.coli K37)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pBR322)(phage f1)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 EcoRI-HindIII 31bp 4360..4361..30
FT DNA polymerase I:
FT 2. phage f1 HaeIII 6407bp 5724..5724, GB #J02448
FT 3. pBR322 EcoRI-HindIII 31bp 4360..4361..30
FT DNA polymerase I:
FT -> fragment 6470bp [HindIII-EcoRI-f1-EcoRI-HindIII]
FT 1. fragment remove small EcoRI-EcoRI 60bp,
FT \ HindIII-HindIII
FT -> R199 6410bp [unique EcoRI]"
FT - 1..5723
FT /note="phage f1 1..5723 5723bp
FT HaeIII = GG^CC
FT EcoRI = G^AATTC"
FT - 5724..5727
FT /note="pBR322 4360..4361..2 4bp
FT EcoRI = G^AATT C
FT HaeIII = GG^CC"
FT - 5728..6411
FT /note="phage f1 5724..6407 684bp"
FT misc_binding 0..0
FT /note="SIT unique EcoRI"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
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SQ Sequence 6411 BP; 1571 A; 1298 C; 1328 G; 2214 T; 0 other;
aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
gttgcatatt taaaacatgt tgagctacag caccagatcc agcaattaag ctctaagcca
tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttgaaacgcg atatttgaag
tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
caatgattaa agttgaaatt aaaccatctc aagcgcaatt cactacccgt tctggtgttt
ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
aatatccggt gcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
tgtacaccgt tcatctgtcc tcgttcaaag ttggtcagtt cggttccctt atgattgacc
gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
cgatcccgca aaagcggcct ttgactccct gcaagcctca gcgaccgaat atatcggtta
tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
tttactaatg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggctgt
ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
tctgagggtg gcggctctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
attccgggtt atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag
gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgttaat
gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt
ggcggttctg agggtggcgg ctctgagggt ggcggttccg gtggcggctc cggttccggt
gattttgatt atgaaaatat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
gctgctatcg acggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
ttaatgaata atttccgtca atatttacct tcccttcctc aatcggttga atgtcgccct
tttgtctttc gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttcgacg
tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg ctaacttttc
ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact
ttgttcaggg cgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg
ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat
cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt
cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat
tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat
acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt
aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg
cgttctgcat tagctgaaaa tgttgtttat tgtcgtcgtc tggacagaat tactttgcct
tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat
gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat
actggtaaga atttgtataa cgcatatgat actaaacagg ctttttccag taattatgat
tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta
aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt
tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg
gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct
cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat
agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc
attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt
tgtttcatca tcttcttttg ctcaagtaat tgaaatgaat aattcgtctc tgcgcgattt
cgttacttgg tattcaaagc aaacaggtga atctgttatt gtctcgcctg atgtaaaggg
tactgtgact gtatattcat ctgacgttaa acctgaaaat ctacgcgatt tctttatttc
tgttttacgt gcaaataatt ttgatatggt tggttcaatc ccttccatta ttcagaagta
taatccaaac aatcaggact atattgatga attgccatca tctgataatc aggaatatga
tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac
ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtagaat tgtttgttaa
atctaatact tctaaatcct caaatgtatt atctgttgac ggctctaatc tattagttgt
tagtgctcct aaagatattt tagataacct tcctcaattc ctttcaactg ttgatttgcc
aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga
tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg
cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt
agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg
tattcttacg ctttcaggtc agaagggttc tatttctgtt ggtcagaatg tcccttttat
tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cggttgagcg
tcaaaatgta ggtatttcca tgagcgtttt tcccgttgca atggctggcg gtaatattgt
tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
tactaatcaa agaagtattg cgacaacggt taatttgcgt gatggacaga ctcttttgct
cggtggcctc actgattata aaaacacttc tcaggattct ggcgtaccgt tcctgtctaa
aatcccttta atcggcctcc tgtttagctc ccgctctgat tctaacgagg aaagcacgtt
atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
attagggtga tggttcacgt agtaattggg ccatcgccct gatagacggt ttttcgccct
ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
acaatttaaa tatttgctta tacaatcttc ctgtttttgg ggcttttctg attatcaacc
ggggtacata tgattgacat gctagtttta cgattaccgt tcatcgattc tcttgtttgc
tccagactct caggcaatga cctgatagcc tttgtagacc tctcaaaaat agctaccctc
tccggcatga atttatcagc tagaacggtt gaatatcata ttgatggtga tttgactgtc
tccggccttt ctcacccgtt tgaatcttta cctacacatt actcaggcat tgcatttaaa
atatatgagg gttctaaaaa tttttatcct tgcgttgaaa taaaggcttc tcccgcaaaa
gtattacagg gtcataatgt ttttggtaca accgatttag ctttatgctc tgaggcttta
ttgcttaatt ttgctaattc tttgccttgc ctgtatgatt tattggatgt t
//