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ID   R199       preliminary; DNA; SYN; 6411 BP.
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AC   ATCC37140;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector f1 R199 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   R199 from phage f1 & pBR322
RC   R208 from R199 & pBR322
RC   R209 from phage f1
RC   R240 from phage f1
RA   Boeke J.D., Vovis G.F., Zinder N.D.;
RT   "Insertion mutant of bacteriophage f1 sensitive to EcoRI";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:2699-2702(1979).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   A filamentous phage DNA vector. (ATCC staff)
CC   Medium is 1592 SM buffer.
CC   NM (R199)
CC   CM (yes)
CC   NA (filamentous ss-DNA)
CC   TP (linear)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (bacteria-free lysate)(E.coli K38)(E.coli K37)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(phage f1)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 EcoRI-HindIII 31bp 4360..4361..30
FT                   DNA polymerase I:
FT                   2. phage f1 HaeIII 6407bp 5724..5724, GB #J02448
FT                   3. pBR322 EcoRI-HindIII 31bp 4360..4361..30
FT                   DNA polymerase I:
FT                   -> fragment 6470bp [HindIII-EcoRI-f1-EcoRI-HindIII]
FT                   1. fragment remove small EcoRI-EcoRI 60bp,
FT                   \ HindIII-HindIII
FT                   -> R199 6410bp [unique EcoRI]"
FT   -               1..5723
FT                   /note="phage f1 1..5723 5723bp
FT                   HaeIII = GG^CC
FT                   EcoRI =   G^AATTC"
FT   -               5724..5727
FT                   /note="pBR322 4360..4361..2 4bp
FT                   EcoRI =   G^AATT C
FT                   HaeIII =      GG^CC"
FT   -               5728..6411
FT                   /note="phage f1 5724..6407 684bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
XX
SQ   Sequence 6411 BP; 1571 A; 1298 C; 1328 G; 2214 T; 0 other;
     aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
     atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
     cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
     gttgcatatt taaaacatgt tgagctacag caccagatcc agcaattaag ctctaagcca
     tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
     ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttgaaacgcg atatttgaag
     tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
     cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
     tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
     aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
     ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
     aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
     atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
     tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
     caatgattaa agttgaaatt aaaccatctc aagcgcaatt cactacccgt tctggtgttt
     ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
     aatatccggt gcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     tgtacaccgt tcatctgtcc tcgttcaaag ttggtcagtt cggttccctt atgattgacc
     gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
     caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     cgatcccgca aaagcggcct ttgactccct gcaagcctca gcgaccgaat atatcggtta
     tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
     tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
     tttactaatg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggctgt
     ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
     tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
     tctgagggtg gcggctctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
     attccgggtt atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
     aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
     cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
     caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
     tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag
     gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgttaat
     gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt
     ggcggttctg agggtggcgg ctctgagggt ggcggttccg gtggcggctc cggttccggt
     gattttgatt atgaaaatat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
     gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
     gctgctatcg acggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
     ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
     ttaatgaata atttccgtca atatttacct tcccttcctc aatcggttga atgtcgccct
     tttgtctttc gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
     ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttcgacg
     tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
     tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg ctaacttttc
     ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
     ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact
     ttgttcaggg cgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
     tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
     attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg
     ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat
     cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt
     cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat
     tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat
     acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt
     aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg
     cgttctgcat tagctgaaaa tgttgtttat tgtcgtcgtc tggacagaat tactttgcct
     tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat
     gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat
     actggtaaga atttgtataa cgcatatgat actaaacagg ctttttccag taattatgat
     tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta
     aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt
     tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg
     gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct
     cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat
     agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc
     attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt
     tgtttcatca tcttcttttg ctcaagtaat tgaaatgaat aattcgtctc tgcgcgattt
     cgttacttgg tattcaaagc aaacaggtga atctgttatt gtctcgcctg atgtaaaggg
     tactgtgact gtatattcat ctgacgttaa acctgaaaat ctacgcgatt tctttatttc
     tgttttacgt gcaaataatt ttgatatggt tggttcaatc ccttccatta ttcagaagta
     taatccaaac aatcaggact atattgatga attgccatca tctgataatc aggaatatga
     tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac
     ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtagaat tgtttgttaa
     atctaatact tctaaatcct caaatgtatt atctgttgac ggctctaatc tattagttgt
     tagtgctcct aaagatattt tagataacct tcctcaattc ctttcaactg ttgatttgcc
     aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga
     tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg
     cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt
     agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg
     tattcttacg ctttcaggtc agaagggttc tatttctgtt ggtcagaatg tcccttttat
     tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cggttgagcg
     tcaaaatgta ggtatttcca tgagcgtttt tcccgttgca atggctggcg gtaatattgt
     tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
     tactaatcaa agaagtattg cgacaacggt taatttgcgt gatggacaga ctcttttgct
     cggtggcctc actgattata aaaacacttc tcaggattct ggcgtaccgt tcctgtctaa
     aatcccttta atcggcctcc tgtttagctc ccgctctgat tctaacgagg aaagcacgtt
     atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     attagggtga tggttcacgt agtaattggg ccatcgccct gatagacggt ttttcgccct
     ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     acaatttaaa tatttgctta tacaatcttc ctgtttttgg ggcttttctg attatcaacc
     ggggtacata tgattgacat gctagtttta cgattaccgt tcatcgattc tcttgtttgc
     tccagactct caggcaatga cctgatagcc tttgtagacc tctcaaaaat agctaccctc
     tccggcatga atttatcagc tagaacggtt gaatatcata ttgatggtga tttgactgtc
     tccggccttt ctcacccgtt tgaatcttta cctacacatt actcaggcat tgcatttaaa
     atatatgagg gttctaaaaa tttttatcct tgcgttgaaa taaaggcttc tcccgcaaaa
     gtattacagg gtcataatgt ttttggtaca accgatttag ctttatgctc tgaggcttta
     ttgcttaatt ttgctaattc tttgccttgc ctgtatgatt tattggatgt t
//