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ID   R229       preliminary; DNA; SYN; 12409 BP.
XX
AC   ATCC37139;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector f1 R229 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   R225, R226, R228, R229, R230 from phage f1 & pBR325
RC   R231, R232, R233 from R225 & pBR325
RC   R236, R237, R238, R239, R240, R248 from phage f1 & pBR325
RC   R250, R251 from R225 & pBR325
RC   R255, R256 from phage f1 & pBR325
RA   Boeke J.D.;
RT   "One and two codon insertion mutants of bacteriophage f1";
RL   Mol. Gen. Genet. 181:288-291(1981).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   A filamentous phage DNA vector with an inserted EcoRI site. Mutation
CC   does not affect plaque-forming ability at higher (43C) temperatures.
CC   Medium is 1592 SM buffer.
CC   NM (R229)
CC   CM (yes)
CC   NA (filamentous ss-DNA)
CC   TP (linear)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (bacteria-free lysate)(E.coli K38)(E.coli K37)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(phage f1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. phage f1 HpaII 6407bp 5614..5614, complete
FT                   Klenow
FT                   2. pBR325 EcoRI 5994bp 4780..4780
FT                   Klenow
FT                   -> R229 12403bp"
FT   -               1..4783
FT                   /note="pBR325 1..4783 4783bp
FT                   EcoRI = G^AATT C
FT                   HpaII =      C^CGG"
FT   -               4784..11192
FT                   /note="phage f1 5614..6407..5615 6409bp
FT                   HpaII = C^CG G
FT                   EcoRI =    G^AATTC"
FT   -               11193..12409
FT                   /note="pBR325 4780..5996 1217bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
XX
SQ   Sequence 12409 BP; 2966 A; 2899 C; 2894 G; 3650 T; 0 other;
     aggccatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg catcaagtga
     ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc
     ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc
     acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa
     acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     ctataaaaat aggcgtatca cgaggccctt tcgtcttcga ataaatacct gtgacggaag
     atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
     acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg
     aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
     ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attcggcttt ccccgtcaag
     ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     cgtttacaat ttaaatattt gcttatacaa tcttcctgtt tttggggctt ttctgattat
     caaccggggt acatatgatt gacatgctag ttttacgatt accgttcatc gattctcttg
     tttgctccag actctcaggc aatgacctga tagcctttgt agacctctca aaaatagcta
     ccctctccgg catgaattta tcagctagaa cggttgaata tcatattgat ggtgatttga
     ctgtctccgg cctttctcac ccgtttgaat ctttacctac acattactca ggcattgcat
     ttaaaatata tgagggttct aaaaattttt atccttgcgt tgaaataaag gcttctcccg
     caaaagtatt acagggtcat aatgtttttg gtacaaccga tttagcttta tgctctgagg
     ctttattgct taattttgct aattctttgc cttgcctgta tgatttattg gatgttaacg
     ctactactat tagtagaatt gatgccacct tttcagctcg cgccccaaat gaaaatatag
     ctaaacaggt tattgaccat ttgcgaaatg tatctaatgg tcaaactaaa tctactcgtt
     cgcagaattg ggaatcaact gttacatgga atgaaacttc cagacaccgt actttagttg
     catatttaaa acatgttgag ctacagcacc agatccagca attaagctct aagccatccg
     caaaaatgac ctcttatcaa aaggagcaat taaaggtact ctctaatcct gacctgttgg
     agtttgcttc cggtctggtt cgctttgaag ctcgaattga aacgcgatat ttgaagtctt
     tcgggcttcc tcttaatctt tttgatgcaa tccgctttgc ttctgactat aatagtcagg
     gtaaagacct gatttttgat ttatggtcat tctcgttttc tgaactgttt aaagcatttg
     agggggattc aatgaatatt tatgacgatt ccgcagtatt ggacgctatc cagtctaaac
     attttactat taccccctct ggcaaaactt cttttgcaaa agcctctcgc tattttggtt
     tttatcgtcg tctggtaaac gagggttatg atagtgttgc tcttactatg cctcgtaatt
     ccttttggcg ttatgtatct gcattagttg aatgtggtat tcctaaatct caactgatga
     atctttctac ctgtaataat gttgttccgt tagttcgttt tattaacgta gatttttctt
     cccaacgtcc tgactggtat aatgagccag ttcttaaaat cgcataaggt aattcacaat
     gattaaagtt gaaattaaac catctcaagc gcaattcact acccgttctg gtgtttctcg
     tcagggcaag ccttattcac tgaatgagca gctttgttac gttgatttgg gtaatgaata
     tccggtgctt gtcaagatta ctcttgatga aggtcagcca gcctatgcgc ctggtctgta
     caccgttcat ctgtcctcgt tcaaagttgg tcagttcggt tcccttatga ttgaccgtct
     gcgcctcgtt ccggctaagt aacatggagc aggtcgcgga tttcgacaca atttatcagg
     cgatgataca aatctccgtt gtactttgtt tcgcgcttgg tataatcgct gggggtcaaa
     gatgagtgtt ttagtgtatt ctttcgcctc tttcgtttta ggttggtgcc ttcgtagtgg
     cattacgtat tttacccgtt taatggaaac ttcctcatga aaaagtcttt agtcctcaaa
     gcctctgtag ccgttgctac cctcgttccg atgctgtctt tcgctgctga gggtgacgat
     cccgcaaaag cggcctttga ctccctgcaa gcctcagcga ccgaatatat cggttatgcg
     tgggcgatgg ttgttgtcat tgtcggcgca actatcggta tcaagctgtt taagaaattc
     acctcgaaag caagctgata aaccgataca attaaaggct ccttttggag cctttttttt
     tggagatttt caacgtgaaa aaattattat tcgcaattcc tttagttgtt cctttctatt
     ctcactccgc tgaaactgtt gaaagttgtt tagcaaaacc ccatacagaa aattcattta
     ctaatgtctg gaaagacgac aaaactttag atcgttacgc taactatgag ggctgtctgt
     ggaatgctac aggcgttgta gtttgtactg gtgacgaaac tcagtgttac ggtacatggg
     ttcctattgg gcttgctatc cctgaaaatg agggtggtgg ctctgagggt ggcggttctg
     agggtggcgg ctctgagggt ggcggtacta aacctcctga gtacggtgat acacctattc
     cgggttatac ttatatcaac cctctcgacg gcacttatcc gcctggtact gagcaaaacc
     ccgctaatcc taatccttct cttgaggagt ctcagcctct taatactttc atgtttcaga
     ataataggtt ccgaaatagg cagggggcat taactgttta tacgggcact gttactcaag
     gcactgaccc cgttaaaact tattaccagt acactcctgt atcatcaaaa gccatgtatg
     acgcttactg gaacggtaaa ttcagagact gcgctttcca ttctggcttt aatgaggatc
     cattcgtttg tgaatatcaa ggccaatcgt ctgacctgcc tcaacctcct gttaatgctg
     gcggcggctc tggtggtggt tctggtggcg gctctgaggg tggtggctct gagggtggcg
     gttctgaggg tggcggctct gagggtggcg gttccggtgg cggctccggt tccggtgatt
     ttgattatga aaatatggca aacgctaata agggggctat gaccgaaaat gccgatgaaa
     acgcgctaca gtctgacgct aaaggcaaac ttgattctgt cgctactgat tacggtgctg
     ctatcgacgg tttcattggt gacgtttccg gccttgctaa tggtaatggt gctactggtg
     attttgctgg ctctaattcc caaatggctc aagtcggtga cggtgataat tcacctttaa
     tgaataattt ccgtcaatat ttaccttccc ttcctcaatc ggttgaatgt cgcccttttg
     tctttcgcgc tggtaaacca tatgaatttt ctattgattg tgacaaaata aacttattcc
     gtggtgtctt tgcgtttctt ttatatgttg ccacctttat gtatgtattt tcgacgtttg
     ctaacatact gcgtaataag gagtcttaat catgccagtt cttttgggta ttccgttatt
     attgcgtttc ctcggtttcc ttctggtaac tttgttcggc tatctgctaa cttttcttaa
     aaagggcttc ggtaagatag ctattgctat ttcattgttt cttgctctta ttattgggct
     taactcaatt cttgtgggtt atctctctga tattagcgct caattaccct ctgactttgt
     tcagggcgtt cagttaattc tcccgtctaa tgcgcttccc tgtttttatg ttattctctc
     tgtaaaggct gctattttca tttttgacgt taaacaaaaa atcgtttctt atttggattg
     ggataaataa tatggctgtt tattttgtaa ctggcaaatt aggctctgga aagacgctcg
     ttagcgttgg taagattcag gataaaattg tagctgggtg caaaatagca actaatcttg
     atttaaggct tcaaaacctc ccgcaagtcg ggaggttcgc taaaacgcct cgcgttctta
     gaataccgga taagccttct atatctgatt tgcttgctat tgggcgcggt aatgattcct
     acgatgaaaa taaaaacggc ttgcttgttc tcgatgagtg cggtacttgg tttaataccc
     gttcttggaa tgataaggaa agacagccga ttattgattg gtttctacat gctcgtaaat
     taggatggga tattattttt cttgttcagg acttatctat tgttgataaa caggcgcgtt
     ctgcattagc tgaaaatgtt gtttattgtc gtcgtctgga cagaattact ttgccttttg
     tcggtacttt atattctctt attactggct cgaaaatgcc tctgcctaaa ttacatgttg
     gcgttgttaa atatggcgat tctcaattaa gccctactgt tgagcgttgg ctttatactg
     gtaagaattt gtataacgca tatgatacta aacaggcttt ttccagtaat tatgattccg
     gtgtttattc ttatttaacg ccttatttat cacacggtcg gtatttcaaa ccattaaatt
     taggtcagaa gatgaaatta actaaaatat atttgaaaaa gttttctcgc gttctttgtc
     ttgcgattgg atttgcatca gcatttacat atagttatat aacccaacct aagccggagg
     ttaaaaaggt agtctctcag acctatgatt ttgataaatt cactattgac tcttctcagc
     gtcttaatct aagctatcgc tatgttttca aggattctaa gggaaaatta attaatagcg
     acgatttaca gaagcaaggt tattcactca catatattga tttatgtact gtttccatta
     aaaaaggtaa ttcaaatgaa attgttaaat gtaattaatt ttgttttctt gatgtttgtt
     tcatcatctt cttttgctca agtaattgaa atgaataatt cgtctctgcg cgatttcgtt
     acttggtatt caaagcaaac aggtgaatct gttattgtct cgcctgatgt aaagggtact
     gtgactgtat attcatctga cgttaaacct gaaaatctac gcgatttctt tatttctgtt
     ttacgtgcaa ataattttga tatggttggt tcaatccctt ccattattca gaagtataat
     ccaaacaatc aggactatat tgatgaattg ccatcatctg ataatcagga atatgatgat
     aattccgctc cttctggtgg tttctttgtt ccgcaaaatg ataatgttac tcaaactttt
     aaaattaata acgttcgggc aaaggattta atacgagttg tagaattgtt tgttaaatct
     aatacttcta aatcctcaaa tgtattatct gttgacggct ctaatctatt agttgttagt
     gctcctaaag atattttaga taaccttcct caattccttt caactgttga tttgccaact
     gaccagatat tgattgaggg tttgatattt gaggttcagc aaggtgatgc tttagatttt
     tcatttgctg ctggctctca gcgtggcact gttgcaggcg gtgttaatac tgaccgcctc
     acctctgttt tatcttctgc tggtggttcg ttcggtattt ttaatggcga tgttttaggg
     ctatcagttc gcgcattaaa gactaatagc cattcaaaaa tattgtctgt gccacgtatt
     cttacgcttt caggtcagaa gggttctatt tctgttggtc agaatgtccc ttttattact
     ggtcgtgtga ctggtgaatc tgccaatgta aataatccat ttcagacggt tgagcgtcaa
     aatgtaggta tttccatgag cgtttttccc gttgcaatgg ctggcggtaa tattgttctg
     gatattacca gcaaggccga tagtttgagt tcttctactc aggcaagtga tgttattact
     aatcaaagaa gtattgcgac aacggttaat ttgcgtgatg gacagactct tttgctcggt
     ggcctcactg attataaaaa cacttctcag gattctggcg taccgttcct gtctaaaatc
     cctttaatcg gcctcctgtt tagctcccgc tctgattcta acgaggaaag cacgttatac
     gtgctcgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     tttcttccct tcctttctcg ccacgttcgc cgaattccgt atggcaatga aagacggtga
     gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
     caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
     cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt
     tttttaaggc agttattggt gcccttaaac gcctggtgct acgcctgaat aagtgataat
     aagcggatga atggcagaaa ttcgaaagca aattcgaccc ggtcgtcggt tcagggcagg
     gtcgttaaat agccgcttat gtctattgct ggtttaccgg tttattgact accggaagca
     gtgtgaccgt gtgcttctca aatgcctgag gccagtttgc tcaggctctc cccgtggagg
     taataattga cgatatgatc atttattctg cctcccagag cctgataaaa acggtgaatc
     cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgac
//