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ID R229 preliminary; DNA; SYN; 12409 BP.
XX
AC ATCC37139;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phage vector f1 R229 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC R225, R226, R228, R229, R230 from phage f1 & pBR325
RC R231, R232, R233 from R225 & pBR325
RC R236, R237, R238, R239, R240, R248 from phage f1 & pBR325
RC R250, R251 from R225 & pBR325
RC R255, R256 from phage f1 & pBR325
RA Boeke J.D.;
RT "One and two codon insertion mutants of bacteriophage f1";
RL Mol. Gen. Genet. 181:288-291(1981).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC A filamentous phage DNA vector with an inserted EcoRI site. Mutation
CC does not affect plaque-forming ability at higher (43C) temperatures.
CC Medium is 1592 SM buffer.
CC NM (R229)
CC CM (yes)
CC NA (filamentous ss-DNA)
CC TP (linear)
CC ST ()
CC TY (phage)
CC SP (ATCC)
CC HO (bacteria-free lysate)(E.coli K38)(E.coli K37)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pBR322)(phage f1)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. phage f1 HpaII 6407bp 5614..5614, complete
FT Klenow
FT 2. pBR325 EcoRI 5994bp 4780..4780
FT Klenow
FT -> R229 12403bp"
FT - 1..4783
FT /note="pBR325 1..4783 4783bp
FT EcoRI = G^AATT C
FT HpaII = C^CGG"
FT - 4784..11192
FT /note="phage f1 5614..6407..5615 6409bp
FT HpaII = C^CG G
FT EcoRI = G^AATTC"
FT - 11193..12409
FT /note="pBR325 4780..5996 1217bp"
FT misc_binding 0..0
FT /note="SIT unique EcoRI"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
XX
SQ Sequence 12409 BP; 2966 A; 2899 C; 2894 G; 3650 T; 0 other;
aggccatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg catcaagtga
ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc
ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc
acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa
acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
ctataaaaat aggcgtatca cgaggccctt tcgtcttcga ataaatacct gtgacggaag
atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg
aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attcggcttt ccccgtcaag
ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
cgtttacaat ttaaatattt gcttatacaa tcttcctgtt tttggggctt ttctgattat
caaccggggt acatatgatt gacatgctag ttttacgatt accgttcatc gattctcttg
tttgctccag actctcaggc aatgacctga tagcctttgt agacctctca aaaatagcta
ccctctccgg catgaattta tcagctagaa cggttgaata tcatattgat ggtgatttga
ctgtctccgg cctttctcac ccgtttgaat ctttacctac acattactca ggcattgcat
ttaaaatata tgagggttct aaaaattttt atccttgcgt tgaaataaag gcttctcccg
caaaagtatt acagggtcat aatgtttttg gtacaaccga tttagcttta tgctctgagg
ctttattgct taattttgct aattctttgc cttgcctgta tgatttattg gatgttaacg
ctactactat tagtagaatt gatgccacct tttcagctcg cgccccaaat gaaaatatag
ctaaacaggt tattgaccat ttgcgaaatg tatctaatgg tcaaactaaa tctactcgtt
cgcagaattg ggaatcaact gttacatgga atgaaacttc cagacaccgt actttagttg
catatttaaa acatgttgag ctacagcacc agatccagca attaagctct aagccatccg
caaaaatgac ctcttatcaa aaggagcaat taaaggtact ctctaatcct gacctgttgg
agtttgcttc cggtctggtt cgctttgaag ctcgaattga aacgcgatat ttgaagtctt
tcgggcttcc tcttaatctt tttgatgcaa tccgctttgc ttctgactat aatagtcagg
gtaaagacct gatttttgat ttatggtcat tctcgttttc tgaactgttt aaagcatttg
agggggattc aatgaatatt tatgacgatt ccgcagtatt ggacgctatc cagtctaaac
attttactat taccccctct ggcaaaactt cttttgcaaa agcctctcgc tattttggtt
tttatcgtcg tctggtaaac gagggttatg atagtgttgc tcttactatg cctcgtaatt
ccttttggcg ttatgtatct gcattagttg aatgtggtat tcctaaatct caactgatga
atctttctac ctgtaataat gttgttccgt tagttcgttt tattaacgta gatttttctt
cccaacgtcc tgactggtat aatgagccag ttcttaaaat cgcataaggt aattcacaat
gattaaagtt gaaattaaac catctcaagc gcaattcact acccgttctg gtgtttctcg
tcagggcaag ccttattcac tgaatgagca gctttgttac gttgatttgg gtaatgaata
tccggtgctt gtcaagatta ctcttgatga aggtcagcca gcctatgcgc ctggtctgta
caccgttcat ctgtcctcgt tcaaagttgg tcagttcggt tcccttatga ttgaccgtct
gcgcctcgtt ccggctaagt aacatggagc aggtcgcgga tttcgacaca atttatcagg
cgatgataca aatctccgtt gtactttgtt tcgcgcttgg tataatcgct gggggtcaaa
gatgagtgtt ttagtgtatt ctttcgcctc tttcgtttta ggttggtgcc ttcgtagtgg
cattacgtat tttacccgtt taatggaaac ttcctcatga aaaagtcttt agtcctcaaa
gcctctgtag ccgttgctac cctcgttccg atgctgtctt tcgctgctga gggtgacgat
cccgcaaaag cggcctttga ctccctgcaa gcctcagcga ccgaatatat cggttatgcg
tgggcgatgg ttgttgtcat tgtcggcgca actatcggta tcaagctgtt taagaaattc
acctcgaaag caagctgata aaccgataca attaaaggct ccttttggag cctttttttt
tggagatttt caacgtgaaa aaattattat tcgcaattcc tttagttgtt cctttctatt
ctcactccgc tgaaactgtt gaaagttgtt tagcaaaacc ccatacagaa aattcattta
ctaatgtctg gaaagacgac aaaactttag atcgttacgc taactatgag ggctgtctgt
ggaatgctac aggcgttgta gtttgtactg gtgacgaaac tcagtgttac ggtacatggg
ttcctattgg gcttgctatc cctgaaaatg agggtggtgg ctctgagggt ggcggttctg
agggtggcgg ctctgagggt ggcggtacta aacctcctga gtacggtgat acacctattc
cgggttatac ttatatcaac cctctcgacg gcacttatcc gcctggtact gagcaaaacc
ccgctaatcc taatccttct cttgaggagt ctcagcctct taatactttc atgtttcaga
ataataggtt ccgaaatagg cagggggcat taactgttta tacgggcact gttactcaag
gcactgaccc cgttaaaact tattaccagt acactcctgt atcatcaaaa gccatgtatg
acgcttactg gaacggtaaa ttcagagact gcgctttcca ttctggcttt aatgaggatc
cattcgtttg tgaatatcaa ggccaatcgt ctgacctgcc tcaacctcct gttaatgctg
gcggcggctc tggtggtggt tctggtggcg gctctgaggg tggtggctct gagggtggcg
gttctgaggg tggcggctct gagggtggcg gttccggtgg cggctccggt tccggtgatt
ttgattatga aaatatggca aacgctaata agggggctat gaccgaaaat gccgatgaaa
acgcgctaca gtctgacgct aaaggcaaac ttgattctgt cgctactgat tacggtgctg
ctatcgacgg tttcattggt gacgtttccg gccttgctaa tggtaatggt gctactggtg
attttgctgg ctctaattcc caaatggctc aagtcggtga cggtgataat tcacctttaa
tgaataattt ccgtcaatat ttaccttccc ttcctcaatc ggttgaatgt cgcccttttg
tctttcgcgc tggtaaacca tatgaatttt ctattgattg tgacaaaata aacttattcc
gtggtgtctt tgcgtttctt ttatatgttg ccacctttat gtatgtattt tcgacgtttg
ctaacatact gcgtaataag gagtcttaat catgccagtt cttttgggta ttccgttatt
attgcgtttc ctcggtttcc ttctggtaac tttgttcggc tatctgctaa cttttcttaa
aaagggcttc ggtaagatag ctattgctat ttcattgttt cttgctctta ttattgggct
taactcaatt cttgtgggtt atctctctga tattagcgct caattaccct ctgactttgt
tcagggcgtt cagttaattc tcccgtctaa tgcgcttccc tgtttttatg ttattctctc
tgtaaaggct gctattttca tttttgacgt taaacaaaaa atcgtttctt atttggattg
ggataaataa tatggctgtt tattttgtaa ctggcaaatt aggctctgga aagacgctcg
ttagcgttgg taagattcag gataaaattg tagctgggtg caaaatagca actaatcttg
atttaaggct tcaaaacctc ccgcaagtcg ggaggttcgc taaaacgcct cgcgttctta
gaataccgga taagccttct atatctgatt tgcttgctat tgggcgcggt aatgattcct
acgatgaaaa taaaaacggc ttgcttgttc tcgatgagtg cggtacttgg tttaataccc
gttcttggaa tgataaggaa agacagccga ttattgattg gtttctacat gctcgtaaat
taggatggga tattattttt cttgttcagg acttatctat tgttgataaa caggcgcgtt
ctgcattagc tgaaaatgtt gtttattgtc gtcgtctgga cagaattact ttgccttttg
tcggtacttt atattctctt attactggct cgaaaatgcc tctgcctaaa ttacatgttg
gcgttgttaa atatggcgat tctcaattaa gccctactgt tgagcgttgg ctttatactg
gtaagaattt gtataacgca tatgatacta aacaggcttt ttccagtaat tatgattccg
gtgtttattc ttatttaacg ccttatttat cacacggtcg gtatttcaaa ccattaaatt
taggtcagaa gatgaaatta actaaaatat atttgaaaaa gttttctcgc gttctttgtc
ttgcgattgg atttgcatca gcatttacat atagttatat aacccaacct aagccggagg
ttaaaaaggt agtctctcag acctatgatt ttgataaatt cactattgac tcttctcagc
gtcttaatct aagctatcgc tatgttttca aggattctaa gggaaaatta attaatagcg
acgatttaca gaagcaaggt tattcactca catatattga tttatgtact gtttccatta
aaaaaggtaa ttcaaatgaa attgttaaat gtaattaatt ttgttttctt gatgtttgtt
tcatcatctt cttttgctca agtaattgaa atgaataatt cgtctctgcg cgatttcgtt
acttggtatt caaagcaaac aggtgaatct gttattgtct cgcctgatgt aaagggtact
gtgactgtat attcatctga cgttaaacct gaaaatctac gcgatttctt tatttctgtt
ttacgtgcaa ataattttga tatggttggt tcaatccctt ccattattca gaagtataat
ccaaacaatc aggactatat tgatgaattg ccatcatctg ataatcagga atatgatgat
aattccgctc cttctggtgg tttctttgtt ccgcaaaatg ataatgttac tcaaactttt
aaaattaata acgttcgggc aaaggattta atacgagttg tagaattgtt tgttaaatct
aatacttcta aatcctcaaa tgtattatct gttgacggct ctaatctatt agttgttagt
gctcctaaag atattttaga taaccttcct caattccttt caactgttga tttgccaact
gaccagatat tgattgaggg tttgatattt gaggttcagc aaggtgatgc tttagatttt
tcatttgctg ctggctctca gcgtggcact gttgcaggcg gtgttaatac tgaccgcctc
acctctgttt tatcttctgc tggtggttcg ttcggtattt ttaatggcga tgttttaggg
ctatcagttc gcgcattaaa gactaatagc cattcaaaaa tattgtctgt gccacgtatt
cttacgcttt caggtcagaa gggttctatt tctgttggtc agaatgtccc ttttattact
ggtcgtgtga ctggtgaatc tgccaatgta aataatccat ttcagacggt tgagcgtcaa
aatgtaggta tttccatgag cgtttttccc gttgcaatgg ctggcggtaa tattgttctg
gatattacca gcaaggccga tagtttgagt tcttctactc aggcaagtga tgttattact
aatcaaagaa gtattgcgac aacggttaat ttgcgtgatg gacagactct tttgctcggt
ggcctcactg attataaaaa cacttctcag gattctggcg taccgttcct gtctaaaatc
cctttaatcg gcctcctgtt tagctcccgc tctgattcta acgaggaaag cacgttatac
gtgctcgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
tttcttccct tcctttctcg ccacgttcgc cgaattccgt atggcaatga aagacggtga
gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt
tttttaaggc agttattggt gcccttaaac gcctggtgct acgcctgaat aagtgataat
aagcggatga atggcagaaa ttcgaaagca aattcgaccc ggtcgtcggt tcagggcagg
gtcgttaaat agccgcttat gtctattgct ggtttaccgg tttattgact accggaagca
gtgtgaccgt gtgcttctca aatgcctgag gccagtttgc tcaggctctc cccgtggagg
taataattga cgatatgatc atttattctg cctcccagag cctgataaaa acggtgaatc
cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgac
//