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ID   RPDR2      preliminary; circular DNA; SYN; 10850 BP.
XX
AC   IG1733;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector rpDR2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   lambda DR2 from lambda
RC   pDR2 from lambda DR2
RA   Murphy A.J., Kung A.L., Swirski R.A., Schimke R.T.;
RT   ;
RL   Unpublished (1992).
XX
CC   NM (rpDR2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)(human fibroblast or lymphoblast cells)
CC   CP ()
CC   FN (cloning cDNA)(expression)
CC   SE ()
CC   PA (EBV)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pDR2 10737bp
FT                   -> rpDR2 10850bp"
FT   misc_binding    0..0
FT                   /note="SIT ClaI"
FT   CDS             0..0
FT                   /note="GEN Epstein-Barr virus nuclear antigen gene
FT                   (EBNA-1)"
FT   rep_origin      0..0
FT                   /note="ORI Epstein-Barr virus (EBV) oriP"
FT   promoter        0..0
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT XboI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 thymidine kinase (TK) gene"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli hygromycin phosphotransferase gene;
FT                   hygromycin resistance gene (hyg)"
FT   promoter        complement(0..0)
FT                   /note="PRO SV40 thymidine kinase (TK) gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 10850 BP; 2723 A; 2615 C; 3053 G; 2459 T; 0 other;
     gtgaccaata caaaacaaaa gcgcccctcg taccagcgaa gaaggggcag agatgccgta
     gtcaggttta gttcgtccgg cggcggggga tctgtatggt gcactctcag tacaatctgc
     tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     atctgagggg actagggtgt gtttaggcga aaagcggggc ttcggttgta cgcggttagg
     agtcccctca ggatatagta gtttcgcttt tgcataggga gggggaaatg tagtcttatg
     caatacactt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     gaaggcaaca gacaggtctg acatggattg gacgaaccac tgaattccgc attgcagaga
     taattgtatt taagtgccta gctcgataca ataaacgcca tttgaccatt caccacattg
     gtgtgcacct ccaagcttgt cgaggaactg aaaaaccaga aagttaactg gtaagtttag
     tctttttgtc ttttatttca ggtcccggat cctctagagt cgacctgcag gcatgcaagc
     ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     ccccaggaag ctcctctgtg tcctcataaa ccctaacctc ctctacttga gaggacattc
     caatcatagg ctgcccatcc accctctgtg tcctcctgtt aattaggtca cttaacaaaa
     aggaaattgg gtaggggttt ttcacagacc gctttctaag ggtaatttta aaatatctgg
     gaagtccctt ccactgctgt gttccagaag tgttggtaaa cagcccacaa atgtcaacag
     cagaaacata caagctgtca gctttgcaca agggcccaac accctgctca tcaagaagca
     ctgtggttgc tgtgttagta atgtgcaaaa caggaggcac attttcccca cctgtgtagg
     ttccaaaata tctagtgttt tcatttttac ttggatcagg aacccagcac tccactggat
     aagcattatc cttatccaaa acagccttgt ggtcagtgtt catctgctga ctgtcaactg
     tagcattttt tggggttaca gtttgagcag gatatttggt cctgtagttt gctaacacac
     cctgcagctc caaaggttcc ccaccaacag caaaaaaatg aaaatttgac ccttgaatgg
     gttttccagc accattttca tgagtttttt gtgtccctga atgcaagttt aacatagcag
     ttaccccaat aacctcagtt ttaacagtaa cagcttccca catcaaaata tttccacagg
     ttaagtcctc atttaaatta ggcaaaggaa ttcctcgacc tgcagcccaa gcttggcact
     ggcgccagaa atccgcgcgg tggtttttgg gggtcggggg tgtttggcag ccacagacgc
     ccggtgttcg tgtcgcgcca gtacatgcgg tccatgccca ggccatccaa aaaccatggg
     tctgtctgct cagtccagtc gtggacctga ccccacgcaa cgcccaaaag aataaccccc
     acgaaccata aaccattccc catgggggac cccgtcccta acccacgggg cccgtggcta
     tggcgggctt gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     ttggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
     ccttccgtgt ttcagttagc ctcccccatc tcccgatccc cacgagtgct ggggcgtcgg
     tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg
     gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc
     tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga
     ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc
     tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg
     gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg
     cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact
     tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg
     acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg
     aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc
     gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca
     gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg
     caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg
     gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt
     acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc
     tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca
     gacgtcgcgg tgagttcagg ctttttcata tcaagctgat cttgcggcac gctgttgacg
     ctgttaagcg ggtcgctgca gggtcgctcg gtgttcgagg ccacacgcgt caccttaata
     tgcgaagtgg acctgggacc gcgccgcccc gactgcatct gcgtgttcga attcgccaat
     gacaagacgc tgggcggggt ttgtgtcatc atagaactaa agacatgcaa atatatttct
     tccggggaca ccgccagcaa acgcgagcaa cgggccacgg ggatgaagca gcccggcggc
     acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
     tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
     gcacgcggcg catctcgggg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
     aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
     gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
     gctcgcggct cttaccagcg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     aggcgtatca cgaggccctt tcgtcttcaa gaattctcat gtttgacagc ttatcatcga
     taagcttggc tggacgtaaa ctcctcttca gacctaataa cttcgtatag catacattat
     acgaagttat attaagggtt attgaatatg atcggaatta tcaagcttat cgataagctg
     atcctcacag gccgcaccca gcttttcttc cgttgcccca gtagcatctc tgtctggtga
     ccttgaagag gaagaggagg ggtcccgaga atccccatcc ctaccgtcca gcaaaaaggg
     ggacgaggaa tttgaggcct ggcttgaggc tcaggacgca aatcttgagg atgttcagcg
     ggagttttcc gggctgcgag taattggtga tgaggacgag gatggttcgg aggatgggga
     attttcagac ctggatctgt ctgacagcga ccatgaaggg gatgagggtg ggggggctgt
     tggagggggc aggagtctgc actccctgta ttcactgagc gtcgtctaat aaagatgtct
     attgatctct tttagtgtga atcatgtctg acgaggggcc aggtacagga cctggaaatg
     gcctaggaga gaagggagac acatctggac cagaaggctc cggcggcagt ggacctcaaa
     gaagaggggg tgataaccat ggacgaggac ggggaagagg acgaggacga ggaggcggaa
     gaccaggagc cccgggcggc tcaggatcag ggccaagaca tagagatggt gtccggagac
     cccaaaaacg tccaagttgc attggctgca aagggaccca cggtggaaca ggagcaggag
     caggagcggg aggggcagga gcaggagggg caggagcagg aggaggggca ggagcaggag
     gaggggcagg aggggcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag
     gaggaggggc aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag
     gggcaggagg ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag
     gaggggcagg agcaggagga ggggcaggag gggcaggagc aggaggaggg gcaggagggg
     caggaggggc aggagcagga ggaggggcag gagcaggagg ggcaggaggg gcaggagggg
     caggagcagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag
     gaggggcagg agcaggaggg gcaggagcag gaggggcagg agcaggaggg gcaggagggg
     caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag
     gaggggcagg aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggggcag
     gaggggcagg agcaggaggg gcaggagggg caggagcagg aggggcagga ggggcaggag
     caggaggagg ggcaggagca ggaggggcag gagcaggagg tggaggccgg ggtcgaggag
     gcagtggagg ccggggtcga ggaggtagtg gaggccgggg tcgaggaggt agtggaggcc
     gccggggtag aggacgtgaa agagccaggg ggggaagtcg tgaaagagcc agggggagag
     gtcgtggacg tggagaaaag aggcccagga gtcccagtag tcagtcatca tcatccgggt
     ctccaccgcg caggccccct ccaggtagaa ggccattttt ccaccctgta ggggaagccg
     attattttga ataccaccaa gaaggtggcc cagatggtga gcctgacgtg cccccgggag
     cgatagagca gggccccgca gatgacccag gagaaggccc aagcactgga ccccggggtc
     agggtgatgg aggcaggcgc aaaaaaggag ggtggtttgg aaagcatcgt ggtcaaggag
     gttccaaccc gaaatttgag aacattgcag aaggtttaag agctctcctg gctaggagtc
     acgtagaaag gactaccgac gaaggaactt gggtcgccgg tgtgttcgta tatggaggta
     gtaagacctc cctttacaac ctaaggcgag gaactgccct tgctattcca caatgtcgtc
     ttacaccatt gagtcgtctc ccctttggaa tggcccctgg acccggccca caacctggcc
     cgctaaggga gtccattgtc tgttatttca tggtcttttt acaaactcat atatttgctg
     aggttttgaa ggatgcgatt aaggaccttg ttatgacaaa gcccgctcct acctgcaata
     tcagggtgac tgtgtgcagc tttgacgatg gagtagattt gcctccctgg tttccaccta
     tggtggaagg ggctgccgcg gagggtgatg acggagatga cggagatgaa ggaggtgatg
     gagatgaggg tgaggaaggg caggagtgat gtaacttgtt aggagacgcc ctcaatcgta
     ttaaaagccg tgtattcccc cgcactaaag aataaatccc cagtagacat catgcgtgct
     gttggtgtat ttctggccat ctgtcttgtc accattttcg tcctcccaac atggggcaat
     tgggcatacc catgttgtca cgtcactcag ctccgcgctc aacaccttct cgcgttggaa
     aacattagcg acatttacct ggtgagcaat cagacatgcg acggctttag cctggcctcc
     ttaaattcac ctaagaatgg gagcaaccag caggaaaagg acaagcagcg aaaattcacg
     cccccttggg aggtggcggc atatgcaaag gatagcactc ccactctact actgggtatc
     atatgctgac tgtatatgca tgaggatagc atatgctacc cggatacaga ttaggatagc
     atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
     ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc
     atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc
     atatgctacc cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat
     gctacccaga tataaattag gatagcatat actaccctaa tctctattag gatagcatat
     gctacccgga tacagattag gatagcatat actacccaga tatagattag gatagcatat
     gctacccaga tatagattag gatagcctat gctacccaga tataaattag gatagcatat
     actacccaga tatagattag gatagcatat gctacccaga tatagattag gatagcctat
     gctacccaga tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta
     cccatggcaa cattagccca ccgtgctctc agcgacctcg tgaatatgag gaccaacaac
     cctgtgcttg gcgctcaggc gcaagtgtgt gtaatttgtc ctccagatcg cagcaatcgc
     gcccctatct tggcccgccc acctacttat gcaggtattc cccggggtgc cattagtggt
     tttgtgggca agtggtttga ccgcagtggt tagcggggtt acaatcagcc aagttattac
     acccttattt tacagtccaa aaccgcaggg cggcgtgtgg gggctgacgc gtgcccccac
     tccacaattt caaaaaaaag agtggccact tgtctttgtt tatgggcccc attggcgtgg
     agccccgttt aattttcggg ggtgttagag acaaccagtg gagtccgctg ctgtcggcgt
     ccactctctt tccccttgtt acaaatagag tgtaacaaca tggttcacct gtcttggtcc
     ctgcctggga cacatcttaa taaccccagt atcatattgc actaggatta tgtgttgccc
     atagccataa attcgtgtga gatggacatc cagtctttac ggcttgtccc caccccatgg
     atttctattg ttaaagatat tcagaatgtt tcattcctac actagtattt attgcccaag
     gggtttgtga gggttatatt ggtgtcatag cacaatgcca ccactgaacc ccccgtccaa
     attttattct gggggcgtca cctgaaacct tgttttcgag cacctcacat acaccttact
     gttcacaact cagcagttat tctattagct aaacgaagga gaatgaagaa gcaggcgaag
     attcaggaga gttcactgcc cgctccttga tcttcagcca ctgcccttgt gactaaaatg
     gttcactacc ctcgtggaat cctgacccca tgtaaataaa accgtgacag ctcatggggt
     gggagatatc gctgttcctt aggacccttt tactaaccct aattcgatag catatgcttc
     ccgttgggta acatatgcta ttgaattagg gttagtctgg atagtatata ctactacccg
     ggaagcatat gctacccgtt tagggttaac aagggggcct tataaacact attgctaatg
     ccctcttgag ggtccgctta tcggtagcta cacaggcccc tctgattgac gttggtgtag
     cctcccgtag tcttcctggg cccctgggag gtacatgtcc cccagcattg gtgtaagagc
     ttcagccaag agttacacat aaaggcaatg ttgtgttgca gtccacagac tgcaaagtct
     gctccaggat gaaagccact cagtgttggc aaatgtgcac atccatttat aaggatgtca
     actacagtca gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc
     ccagttggca agttgtacca accaactgaa gggattacat gcactgcccc
//