back Return to this vector's summary.
ID   RSC13      preliminary; circular DNA; SYN; 7894 BP.
XX
AC   J01783; V00351;
XX
DT   03-MAY-1985 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector Rsc13 - complete.
XX
KW   cloning vector; FII plasmid.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   646-1189
RC   from pSM1
RC   from pSM2
RC   from pSM15
RC   from Rsc13
RA   Rosen J.I., Ryder T.B., Inokuchi H., Ohtsubo H., Ohtsubo E.;
RT   "Genes and sites involved in replication and incompatibility of an
RT   R100 plasmid derivative based on nucleotide sequence analysis";
RL   Mol. Gen. Genet. 179:527-537(1980).
XX
RN   [2]
RP   1-2982; 7850-7894
RC   pKN102 from R1
RC   Rsc13 from pKN102
RC   pTR1 from Rsc13
RC   pSM1 from R100
RA   Ryder T.B., Davison D.B., Rosen J.I., Ohtsubo E., Ohtsubo H.;
RT   "Analysis of plasmid genome evolution based on nucleotide-sequence
RT   comparison of two related plasmids of Escherichia coli";
RL   Gene 17:299-310(1982).
XX
RN   [3]
RP   1-7894
RC   Rsc13 from pKN102
RA   Davison D.B.;
RT   ;
RL   Unpublished (1984).
XX
RN   [4]
RP   1-7894
RC   R1 from E. coli IncFII group
RC   pGW15 from CopA gene
RC   pGW58-10, pGW58-15 from pGW58
RC   pGW133, pGW233 from pGW58 & pMC1403
RC   pGW58-L from pGW58
RC   pGW177-L from pGW58-L
RC   pGW277 from pGW177-L
RC   NR1, RepFIC/F, RepIFC/P307, pSU316, pSU233 from IncFII plasmids
RC   pSU221, pINV, ColV2-K94, pYVe439-80RA from IncFII plasmids
RA   Blomberg P., Nordstrom K., Wagner E.G.;
RT   "Replication control of plasmid R1: RepA synthesis is regulated by
RT   CopA RNA through inhibition of leader peptide translation";
RL   EMBO J. 11:2675-2683(1992).
XX
RN   [5]
RC   F'lac::Tn3 from F' lac & Rsc11, Tn3
RC   Rsc11, Rsc13 from R1drd-19B2
RC   R1drd-16 from R1drd-19B2
RC   R1drd-16::Tn3 from R1drd-16 & Rsc11, Tn3
RC   pWL625 from pML21 & Rsc13
RC   pWL803. pWL804, pWL812, pWL815 from pWL625
RA   Goebel W., Lindenmaier W., Pfeiffer F., Schrempf H., Schelle B.;
RT   "Transposition and insertion of intact, deleted and enlarged
RT   ampicillin transposon Tn3 from mini-R1 (Rsc) plasmids into
RT   transfer factors";
RL   Mol. Gen. Genet. 157:119-129(1977).
XX
RN   [6]
RC   pKN102 or R1drd-19B2 from R1drd-19
RC   Rsc10, Rsc11, Rsc12, Rsc13, Rsc14 from pKN102 or R1drd-19B2
RC   pWB5 from ColE1 & Rsc11
RC   pWL812 from pKN102
RA   Goebel W., Bonewald R.;
RT   "Class of small multicopy plasmids originating from the mutant
RT   antibiotic resistance factor R1drd-19B2";
RL   J. Bacteriol. 123:658-665(1975).
XX
CC   Complete plasmid was not displayed in the published papers [1] [2] but
CC   was kindly supplied with annotation by Davison, one of the authors.
CC   NM (Rsc13)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (R1)(Tn3)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. R1
FT                   -> R1drd-19
FT                   1. R1drd-19, replication region
FT                   mutagenesis
FT                   -> pKN102
FT                   1. pKN102 2937bp, R1 ori/inc 80.2 to 83.0
FT                   transposition
FT                   2. Tn3 4957bp, complete with amp/tnpR/tnpA
FT                   -> Rsc13 7894bp"
FT   CDS             407..667
FT                   /note="GEN E. coli repA2 gene"
FT   CDS             complement(693..845)
FT                   /note="GEN E. coli 5.3 kd gene; putative"
FT   misc_RNA        706..>706
FT                   /note="RNA E. coli RNAII gene,
FT                   (may be message for repA3 and repA1)"
FT   CDS             761..946
FT                   /note="GEN E. coli repA3 gene; putative"
FT   misc_RNA        complement(784..874)
FT                   /note="RNA E. coli RNAI gene,
FT                   (may be message for 5.3K protein)"
FT   CDS             959..1816
FT                   /note="GEN E. coli repA1 gene"
FT   CDS             2179..2565
FT                   /note="GEN E. coli repA4 gene; putative"
FT   misc_RNA        2627..2777
FT                   /note="RNA E. coli RNAIII gene,
FT                   (unknown function)"
FT   transposon      2938..7893
FT                   /note="TRN transposon Tn3"
FT   rep_origin      0..0
FT                   /note="ORI E. coli plasmid R1"
XX
SQ   Sequence 7894 BP; 2001 A; 1982 C; 1969 G; 1942 T; 0 other;
     atttacgacc tgaacgcaga cgccgggata ttcttctgga agaggttctg aggcaggctg
     gtattccgtt gctcagaagt cacgatgcca gaaaactgct gcagatgacc ggagaatggc
     tgaatacaac aggggctgct cagcagtcac cggaacatcg tagctgacgc cttcgcgttg
     ctcggttgtc caaccccgga aacgggaaaa agcaagtttt ccccgctccc ggcgtttcca
     taactgaaaa ccatactatt tcacagttta aatcacatta aacgacagta atccccgttg
     atttgtgcgc caacacagat cttcgtcaca attctcaagt cgctgatttc aaaaaactgt
     agtatcctct gcgaaacgat ccctgtttga gtattgagga ggcgagatgt cgcagacaga
     aaatgcagtg acttcctcat tgagtcaaaa gcggtttgtg cgcagaggta agcctatgac
     tgactctgag aaacaaatgg ccgctgttgc aagaaaacgt cttacacaca aagagataaa
     agtttttgtc aaaaatcctc tgaaagatct catggttgag tactgcgaga gagaggggat
     aacacaggct cagttcgttg agaaaatcat caaagatgaa ctgcagagac tggatatact
     aaagtaaaga ctttactttg tggcgtagca tgctagatta ctgatcgttt aaggaatttt
     gtggctggcc acgccgtaag gtggcaagga actggttctg atgtggattt acaggagcca
     gaaaagcaaa aaccccgata atcttcttca actttggcga atacgaaaag attaccgggg
     cccacttaaa ccgtatagcc aacaattcag ctatgcgggg agtatagtta tatgcccgga
     aaagttcaag acttctttct gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt
     gactgatctt caccaaacgt attaccgcca ggtaaagaac ccgaatccgg tgttcactcc
     ccgtgaaggt gccggaacgc tgaagttctg cgaaaaactg atggaaaagg cggtgggctt
     cacctcccgt tttgatttcg ccattcatgt ggcgcatgcc cgttcccgtg gtctgcgtcg
     gcgcatgcca ccggtgctgc gtcgacgggc tattgatgcg ctgctgcagg ggctgtgttt
     ccactatgac ccgctggcca accgcgtcca gtgttccatc accacactgg ccattgagtg
     cggactggcg acagagtccg gtgcaggaaa actctccatc acccgtgcca cccgggccct
     gacgttcctg tcagagctgg gactgattac ctaccagacg gaatatgacc cgcttatcgg
     gtgctacatt ccgaccgaca tcacgttcac actggctctg tttgctgccc ttgatgtgtc
     tgaggatgca gtggcagctg cgcgccgcag tcgtgttgaa tgggaaaaca aacagcgcaa
     aaagcagggg ctggataccc tgggtatgga tgagctgata gcgaaagcct ggcgttttgt
     gcgtgagcgt ttccgcagtt accagacaga gcttcagtcc cgtggaataa aacgtgcccg
     tgcgcgtcgt gatgcgaaca gagaacgtca ggatatcgtc accctagtga aacggcagct
     gacgcgtgaa atctcggaag gacgcttcac tgctaatggt gaggcggtaa aacgcgaagt
     ggagcgtcgt gtgaaggagc gcatgattct gtcacgtaac cgcaattaca gccggctggc
     cacagcttct ccctgaaagt gatctcctca gaataatccg gcctgcgccg gaggcatccg
     cacgcctgaa gcccgccggt gcacaaaaaa acagcgtcgc atgcaaaaaa caatctcatc
     atccaccttc tggagcatcc gattccccct gtttttaata caaaatacgc ctcagcgacg
     gggaattttg cttatccaca tttaactgca agggacttcc ccataaggtt acaaccgttc
     atgtcataaa gcgccagccg ccagtcttac agggtgcaat gtatctttta aacacctgtt
     tatatctcct ttaaactact taattacatt catttaaaaa gaaaacctat tcactgcctg
     tcctgtggac agacagatat gcacctccca ccgcaagcgg cgggccccga ccggagccac
     tttagttaca acacacaaaa acaacctcca gaaaaacccc ggtccagcgc agaaccgaaa
     ccacaaagcc cctccctcat aactgaaaag cggccccgcc ccggcccaaa gggccggaac
     agagtcgctt ttaattatga atgttgtaac tacatcttca tcgctgtcag tcttctcgct
     ggaagttctc agtacacgct cgtaagcggc cctcacggcc cgctaacgcg gagacacgcc
     ccgacttcgg gtaaaccctc gtcgggacca ctccgaccgc gcacagaagc tctctcatgg
     ctgaaagcgg gtatggtctg gcagggctgg ggatgggtaa ggtgaaatct atcaatcagt
     accggcttac gccgggcttc ggcggtttta ctcctgtatc atatgaaaca acagagtgcc
     gccttccatg ccgctgatgc ggcatatcct ggtaacgata tctgaattgt tatacatgtg
     tatatacgtg gtaatgacaa aaataggaca agttaaaaat ttacaggcga tgcaatgatt
     caaacacgta atcaatatct gcagtttatg ctggttatgc tggctgcatg gggcattagt
     tcgggagcca gatttgtcat ggagcaggcc gttctgcttt atggatcagg aaaaaactat
     ttgttcttca gtcatggtac tgttctgatg tacctgctgt gtgttttcct ggtatacggg
     gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     tcgtcttcaa gaattttata aaccgtggag cgggcaatac tgagctgatg agcaatttcc
     gttgcaccag tgcccttctg atgaagcgtc agcacgacgt tcctgtccac ggtacgcctg
     cggccaaatt tgattccttt cagctttgct tcctgtcggc cctcattcgt gcgctctagg
     atcctccggc gttcagcctg tgccacagcc gacaggatgg tgaccaccat ttgccccata
     tcaccgtcgg tactgatccc gtcgtcaata aaccgaaccg ctacaccctg agcatcaaac
     tcttttatca gttggatcat gtcggcggtg tcgcggccaa gacggtcgag cttcttcacc
     agaatgacat caccttcctc caccttcatc ctcagcaaat ccagcccttc ccgatctgtt
     gaactgccgg atgccttgtc ggtaaagatg cggttagctt ttacccctgc atctttgagc
     gctctgatct gaatatcgag ggactgctgg ctggttgaga cccgcgcata accaaaaatt
     cgcataaaaa tgtaccttaa atcgaatatc agacacgatg tgtctattat gccaaaatga
     cgatttaatg gacactcaaa cgaagccgtt ttactatgtc tgataattta taatatttcg
     aacggttgca gttgtgttaa aaaagccgtc aggcagggag gccgatatgc ccgttgattt
     tttgaccact gagcaggttg agagttatgg caggttcact ggcgaacccg atgaacttca
     gctggcgcgt tattttcatc ttgatgaagc ggataaagaa tttatcggga aaagccgggg
     tgatcacaat cgacttggta ttgccctgca aatcgggtgt gtgcgttttc tgggcacttt
     tcttactgac atgaatcata ttccttccgg cgtccggcat tttaccgcca gacagctcgg
     gattcgtgat atcaccgttc ttgcagaata cggtcagagg gaaaataccc gccgtgagca
     tgcagcgctg atacgtcagc actatcagta tcgtgaattt gcctggccct ggacatttcg
     ccttacccgt cttttatata cccggagctg gataagcaac gaacgtcctg gcctgctttt
     cgacctggcg acagggtggc ttatgcaaca tcgtattatt ctccccggag ccaccacgct
     gacccggttg atttcagagg taagggaaaa ggcgacgttg cgcctgtgga acaaactggc
     actgataccg tcagccgaac agcgttcaca gctggagatg ctgctggggc caactgattg
     cagccgcctg tctttactgg aatcactgaa aaaaggccct gtgaccatca gtggtccggc
     gtttaatgaa gcaattgaac gctggaaaac tctgaacgat tttggcctgc atgctgaaaa
     cctgagtaca ctcccggctg tgcgcctgaa aaatctcgca cgttatgctg gtatgacttc
     ggtgttcaat attgccagga tgtcaccgca gaaaaggatg gcggttctgg ttgcctttgt
     ccttgcatgg gaaacgctgg cgctggatga tgcactggac gttctggacg ccatgctggc
     cgttatcatc cgtgacgcca gaaagattgg gcagaaaaaa cggctccgct cgctgaagga
     tctggataaa tctgcattgg cgctcgccag cgcatgttcg tacttgctga aagaagaaac
     accggacgaa tcgattcgtg ctgaggtgtt cagctacatc cctaggcaaa agctggctga
     aatcatcacg cttgtccgtg aaattgcccg gccctcagac gataattttc atgacgaaat
     ggtggagcag tacgggcgcg ttcgtcgttt cctgccccat ctgctgaata ccgttaaatt
     ttcatccgca cctgccgggg ttaccactct gaatgcctgt gactacctca gccgggagtt
     cagctcacgg cggcagtttt ttgacgacgc accaacggaa atcatcagtc agtcatggaa
     acggctggtg attaacaagg aaaaacatat cacccgaagg ggatacacgc tctgctttct
     cagtaaactg caggatagtc tgagacggag ggatgtctac gttaccggca gtaaccggtg
     gggagatcct cgtgcaagat tactacaggg tgctgactgg caggcaaatc ggattaaggt
     ttatcgttct ttggggcacc cgacagaccc gcaggaagca ataaaatctc tgggccatca
     gcttgatagt cgttacagac aggttgctgc acgtcttggc gaaaatgagg ctgtcgaact
     cgatgtttct ggcccgaagc cccggttgac aatttctccc ctcgccagtc ttgatgagcc
     ggacagtctg aaacgactga gcaaaatgat cagtgatctg ctccctccgg tggatttaac
     ggagttgctg ctcgaaatta acgcccatac cggatttgct gatgagtttt tccatgccag
     tgaagccagt gccagagttg atgatctgcc cgtcagcatc agcgccgtgc tgatggctga
     agcctgcaat atcggtctgg aaccactgat cagatcaaat gttcctgcac tgacccgaca
     ccggctgaac tggacaaaag cgaactatct gcgggctgaa actatcacca gcgctaatgc
     cagactggtt gattttcagg caacgctgcc actggcacag atatggggtg gaggagaagt
     ggcatctgca gatggaatgc gctttgttac gccagtcaga acaatcaatg ccggaccgaa
     ccgcaaatac tttggtaata acagagggat cacctggtac aactttgtgt ccgatcagta
     ttccggcttt catggctttc atggcatcgt tataccgggg acgctgaggg actctatctt
     tgtgctggaa ggccttctgg aacaggagac cgggctgaat ccaaccgaaa ttatgaccga
     tacggcaggt gccagcgatc ttgtctttgg ccttttctgg ctgctgggat accagttttc
     tccacgcctg gctgatgccg gtgcttcggt tttctggcga atggaccatg atgccgacta
     tggcgtgctg aatgatattg ccagagggca atcagatccc cgaaaaatag tccttcagtg
     ggacgaaatg atccggaccg caggctccct gaagctgggc aaagtacagg cctcagtgct
     ggtccgttca ttgctgaaaa gtgaacgtcc ctccggactg actcaggcaa tcattgaagt
     ggggcgcatc aacaaaacgc tgtatctgct taattatatt gatgatgaag attaccgccg
     gcgcattctg acccagctta atcggggaga aagccgtcat gcagttgcca gagccatctg
     tcacggtcaa aaaggtgaga taagaaaacg atataccgac ggtcaggaag atcagttggg
     agctctgggg ctggtcacta acgccgtcgt gttatggaac actatttata tgcaggcagc
     tctggatcat ctccgggcgc agggtgaaac actgaatgat gaagatatcg cacggctctc
     cccgctttgc cacggacata tcaatatgct cggccattat tccttcacgc tggcagaact
     ggtgaccaaa ggtcatctga gaccattaaa agaggcgtca gaggcagaaa acgttgctta
     acgtgagttt tcgttccact gagcgtcaga cccc
//