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ID RSC13 preliminary; circular DNA; SYN; 7894 BP.
XX
AC J01783; V00351;
XX
DT 03-MAY-1985 (Rel. 1, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector Rsc13 - complete.
XX
KW cloning vector; FII plasmid.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 646-1189
RC from pSM1
RC from pSM2
RC from pSM15
RC from Rsc13
RA Rosen J.I., Ryder T.B., Inokuchi H., Ohtsubo H., Ohtsubo E.;
RT "Genes and sites involved in replication and incompatibility of an
RT R100 plasmid derivative based on nucleotide sequence analysis";
RL Mol. Gen. Genet. 179:527-537(1980).
XX
RN [2]
RP 1-2982; 7850-7894
RC pKN102 from R1
RC Rsc13 from pKN102
RC pTR1 from Rsc13
RC pSM1 from R100
RA Ryder T.B., Davison D.B., Rosen J.I., Ohtsubo E., Ohtsubo H.;
RT "Analysis of plasmid genome evolution based on nucleotide-sequence
RT comparison of two related plasmids of Escherichia coli";
RL Gene 17:299-310(1982).
XX
RN [3]
RP 1-7894
RC Rsc13 from pKN102
RA Davison D.B.;
RT ;
RL Unpublished (1984).
XX
RN [4]
RP 1-7894
RC R1 from E. coli IncFII group
RC pGW15 from CopA gene
RC pGW58-10, pGW58-15 from pGW58
RC pGW133, pGW233 from pGW58 & pMC1403
RC pGW58-L from pGW58
RC pGW177-L from pGW58-L
RC pGW277 from pGW177-L
RC NR1, RepFIC/F, RepIFC/P307, pSU316, pSU233 from IncFII plasmids
RC pSU221, pINV, ColV2-K94, pYVe439-80RA from IncFII plasmids
RA Blomberg P., Nordstrom K., Wagner E.G.;
RT "Replication control of plasmid R1: RepA synthesis is regulated by
RT CopA RNA through inhibition of leader peptide translation";
RL EMBO J. 11:2675-2683(1992).
XX
RN [5]
RC F'lac::Tn3 from F' lac & Rsc11, Tn3
RC Rsc11, Rsc13 from R1drd-19B2
RC R1drd-16 from R1drd-19B2
RC R1drd-16::Tn3 from R1drd-16 & Rsc11, Tn3
RC pWL625 from pML21 & Rsc13
RC pWL803. pWL804, pWL812, pWL815 from pWL625
RA Goebel W., Lindenmaier W., Pfeiffer F., Schrempf H., Schelle B.;
RT "Transposition and insertion of intact, deleted and enlarged
RT ampicillin transposon Tn3 from mini-R1 (Rsc) plasmids into
RT transfer factors";
RL Mol. Gen. Genet. 157:119-129(1977).
XX
RN [6]
RC pKN102 or R1drd-19B2 from R1drd-19
RC Rsc10, Rsc11, Rsc12, Rsc13, Rsc14 from pKN102 or R1drd-19B2
RC pWB5 from ColE1 & Rsc11
RC pWL812 from pKN102
RA Goebel W., Bonewald R.;
RT "Class of small multicopy plasmids originating from the mutant
RT antibiotic resistance factor R1drd-19B2";
RL J. Bacteriol. 123:658-665(1975).
XX
CC Complete plasmid was not displayed in the published papers [1] [2] but
CC was kindly supplied with annotation by Davison, one of the authors.
CC NM (Rsc13)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (R1)(Tn3)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. R1
FT -> R1drd-19
FT 1. R1drd-19, replication region
FT mutagenesis
FT -> pKN102
FT 1. pKN102 2937bp, R1 ori/inc 80.2 to 83.0
FT transposition
FT 2. Tn3 4957bp, complete with amp/tnpR/tnpA
FT -> Rsc13 7894bp"
FT CDS 407..667
FT /note="GEN E. coli repA2 gene"
FT CDS complement(693..845)
FT /note="GEN E. coli 5.3 kd gene; putative"
FT misc_RNA 706..>706
FT /note="RNA E. coli RNAII gene,
FT (may be message for repA3 and repA1)"
FT CDS 761..946
FT /note="GEN E. coli repA3 gene; putative"
FT misc_RNA complement(784..874)
FT /note="RNA E. coli RNAI gene,
FT (may be message for 5.3K protein)"
FT CDS 959..1816
FT /note="GEN E. coli repA1 gene"
FT CDS 2179..2565
FT /note="GEN E. coli repA4 gene; putative"
FT misc_RNA 2627..2777
FT /note="RNA E. coli RNAIII gene,
FT (unknown function)"
FT transposon 2938..7893
FT /note="TRN transposon Tn3"
FT rep_origin 0..0
FT /note="ORI E. coli plasmid R1"
XX
SQ Sequence 7894 BP; 2001 A; 1982 C; 1969 G; 1942 T; 0 other;
atttacgacc tgaacgcaga cgccgggata ttcttctgga agaggttctg aggcaggctg
gtattccgtt gctcagaagt cacgatgcca gaaaactgct gcagatgacc ggagaatggc
tgaatacaac aggggctgct cagcagtcac cggaacatcg tagctgacgc cttcgcgttg
ctcggttgtc caaccccgga aacgggaaaa agcaagtttt ccccgctccc ggcgtttcca
taactgaaaa ccatactatt tcacagttta aatcacatta aacgacagta atccccgttg
atttgtgcgc caacacagat cttcgtcaca attctcaagt cgctgatttc aaaaaactgt
agtatcctct gcgaaacgat ccctgtttga gtattgagga ggcgagatgt cgcagacaga
aaatgcagtg acttcctcat tgagtcaaaa gcggtttgtg cgcagaggta agcctatgac
tgactctgag aaacaaatgg ccgctgttgc aagaaaacgt cttacacaca aagagataaa
agtttttgtc aaaaatcctc tgaaagatct catggttgag tactgcgaga gagaggggat
aacacaggct cagttcgttg agaaaatcat caaagatgaa ctgcagagac tggatatact
aaagtaaaga ctttactttg tggcgtagca tgctagatta ctgatcgttt aaggaatttt
gtggctggcc acgccgtaag gtggcaagga actggttctg atgtggattt acaggagcca
gaaaagcaaa aaccccgata atcttcttca actttggcga atacgaaaag attaccgggg
cccacttaaa ccgtatagcc aacaattcag ctatgcgggg agtatagtta tatgcccgga
aaagttcaag acttctttct gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt
gactgatctt caccaaacgt attaccgcca ggtaaagaac ccgaatccgg tgttcactcc
ccgtgaaggt gccggaacgc tgaagttctg cgaaaaactg atggaaaagg cggtgggctt
cacctcccgt tttgatttcg ccattcatgt ggcgcatgcc cgttcccgtg gtctgcgtcg
gcgcatgcca ccggtgctgc gtcgacgggc tattgatgcg ctgctgcagg ggctgtgttt
ccactatgac ccgctggcca accgcgtcca gtgttccatc accacactgg ccattgagtg
cggactggcg acagagtccg gtgcaggaaa actctccatc acccgtgcca cccgggccct
gacgttcctg tcagagctgg gactgattac ctaccagacg gaatatgacc cgcttatcgg
gtgctacatt ccgaccgaca tcacgttcac actggctctg tttgctgccc ttgatgtgtc
tgaggatgca gtggcagctg cgcgccgcag tcgtgttgaa tgggaaaaca aacagcgcaa
aaagcagggg ctggataccc tgggtatgga tgagctgata gcgaaagcct ggcgttttgt
gcgtgagcgt ttccgcagtt accagacaga gcttcagtcc cgtggaataa aacgtgcccg
tgcgcgtcgt gatgcgaaca gagaacgtca ggatatcgtc accctagtga aacggcagct
gacgcgtgaa atctcggaag gacgcttcac tgctaatggt gaggcggtaa aacgcgaagt
ggagcgtcgt gtgaaggagc gcatgattct gtcacgtaac cgcaattaca gccggctggc
cacagcttct ccctgaaagt gatctcctca gaataatccg gcctgcgccg gaggcatccg
cacgcctgaa gcccgccggt gcacaaaaaa acagcgtcgc atgcaaaaaa caatctcatc
atccaccttc tggagcatcc gattccccct gtttttaata caaaatacgc ctcagcgacg
gggaattttg cttatccaca tttaactgca agggacttcc ccataaggtt acaaccgttc
atgtcataaa gcgccagccg ccagtcttac agggtgcaat gtatctttta aacacctgtt
tatatctcct ttaaactact taattacatt catttaaaaa gaaaacctat tcactgcctg
tcctgtggac agacagatat gcacctccca ccgcaagcgg cgggccccga ccggagccac
tttagttaca acacacaaaa acaacctcca gaaaaacccc ggtccagcgc agaaccgaaa
ccacaaagcc cctccctcat aactgaaaag cggccccgcc ccggcccaaa gggccggaac
agagtcgctt ttaattatga atgttgtaac tacatcttca tcgctgtcag tcttctcgct
ggaagttctc agtacacgct cgtaagcggc cctcacggcc cgctaacgcg gagacacgcc
ccgacttcgg gtaaaccctc gtcgggacca ctccgaccgc gcacagaagc tctctcatgg
ctgaaagcgg gtatggtctg gcagggctgg ggatgggtaa ggtgaaatct atcaatcagt
accggcttac gccgggcttc ggcggtttta ctcctgtatc atatgaaaca acagagtgcc
gccttccatg ccgctgatgc ggcatatcct ggtaacgata tctgaattgt tatacatgtg
tatatacgtg gtaatgacaa aaataggaca agttaaaaat ttacaggcga tgcaatgatt
caaacacgta atcaatatct gcagtttatg ctggttatgc tggctgcatg gggcattagt
tcgggagcca gatttgtcat ggagcaggcc gttctgcttt atggatcagg aaaaaactat
ttgttcttca gtcatggtac tgttctgatg tacctgctgt gtgttttcct ggtatacggg
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
tcgtcttcaa gaattttata aaccgtggag cgggcaatac tgagctgatg agcaatttcc
gttgcaccag tgcccttctg atgaagcgtc agcacgacgt tcctgtccac ggtacgcctg
cggccaaatt tgattccttt cagctttgct tcctgtcggc cctcattcgt gcgctctagg
atcctccggc gttcagcctg tgccacagcc gacaggatgg tgaccaccat ttgccccata
tcaccgtcgg tactgatccc gtcgtcaata aaccgaaccg ctacaccctg agcatcaaac
tcttttatca gttggatcat gtcggcggtg tcgcggccaa gacggtcgag cttcttcacc
agaatgacat caccttcctc caccttcatc ctcagcaaat ccagcccttc ccgatctgtt
gaactgccgg atgccttgtc ggtaaagatg cggttagctt ttacccctgc atctttgagc
gctctgatct gaatatcgag ggactgctgg ctggttgaga cccgcgcata accaaaaatt
cgcataaaaa tgtaccttaa atcgaatatc agacacgatg tgtctattat gccaaaatga
cgatttaatg gacactcaaa cgaagccgtt ttactatgtc tgataattta taatatttcg
aacggttgca gttgtgttaa aaaagccgtc aggcagggag gccgatatgc ccgttgattt
tttgaccact gagcaggttg agagttatgg caggttcact ggcgaacccg atgaacttca
gctggcgcgt tattttcatc ttgatgaagc ggataaagaa tttatcggga aaagccgggg
tgatcacaat cgacttggta ttgccctgca aatcgggtgt gtgcgttttc tgggcacttt
tcttactgac atgaatcata ttccttccgg cgtccggcat tttaccgcca gacagctcgg
gattcgtgat atcaccgttc ttgcagaata cggtcagagg gaaaataccc gccgtgagca
tgcagcgctg atacgtcagc actatcagta tcgtgaattt gcctggccct ggacatttcg
ccttacccgt cttttatata cccggagctg gataagcaac gaacgtcctg gcctgctttt
cgacctggcg acagggtggc ttatgcaaca tcgtattatt ctccccggag ccaccacgct
gacccggttg atttcagagg taagggaaaa ggcgacgttg cgcctgtgga acaaactggc
actgataccg tcagccgaac agcgttcaca gctggagatg ctgctggggc caactgattg
cagccgcctg tctttactgg aatcactgaa aaaaggccct gtgaccatca gtggtccggc
gtttaatgaa gcaattgaac gctggaaaac tctgaacgat tttggcctgc atgctgaaaa
cctgagtaca ctcccggctg tgcgcctgaa aaatctcgca cgttatgctg gtatgacttc
ggtgttcaat attgccagga tgtcaccgca gaaaaggatg gcggttctgg ttgcctttgt
ccttgcatgg gaaacgctgg cgctggatga tgcactggac gttctggacg ccatgctggc
cgttatcatc cgtgacgcca gaaagattgg gcagaaaaaa cggctccgct cgctgaagga
tctggataaa tctgcattgg cgctcgccag cgcatgttcg tacttgctga aagaagaaac
accggacgaa tcgattcgtg ctgaggtgtt cagctacatc cctaggcaaa agctggctga
aatcatcacg cttgtccgtg aaattgcccg gccctcagac gataattttc atgacgaaat
ggtggagcag tacgggcgcg ttcgtcgttt cctgccccat ctgctgaata ccgttaaatt
ttcatccgca cctgccgggg ttaccactct gaatgcctgt gactacctca gccgggagtt
cagctcacgg cggcagtttt ttgacgacgc accaacggaa atcatcagtc agtcatggaa
acggctggtg attaacaagg aaaaacatat cacccgaagg ggatacacgc tctgctttct
cagtaaactg caggatagtc tgagacggag ggatgtctac gttaccggca gtaaccggtg
gggagatcct cgtgcaagat tactacaggg tgctgactgg caggcaaatc ggattaaggt
ttatcgttct ttggggcacc cgacagaccc gcaggaagca ataaaatctc tgggccatca
gcttgatagt cgttacagac aggttgctgc acgtcttggc gaaaatgagg ctgtcgaact
cgatgtttct ggcccgaagc cccggttgac aatttctccc ctcgccagtc ttgatgagcc
ggacagtctg aaacgactga gcaaaatgat cagtgatctg ctccctccgg tggatttaac
ggagttgctg ctcgaaatta acgcccatac cggatttgct gatgagtttt tccatgccag
tgaagccagt gccagagttg atgatctgcc cgtcagcatc agcgccgtgc tgatggctga
agcctgcaat atcggtctgg aaccactgat cagatcaaat gttcctgcac tgacccgaca
ccggctgaac tggacaaaag cgaactatct gcgggctgaa actatcacca gcgctaatgc
cagactggtt gattttcagg caacgctgcc actggcacag atatggggtg gaggagaagt
ggcatctgca gatggaatgc gctttgttac gccagtcaga acaatcaatg ccggaccgaa
ccgcaaatac tttggtaata acagagggat cacctggtac aactttgtgt ccgatcagta
ttccggcttt catggctttc atggcatcgt tataccgggg acgctgaggg actctatctt
tgtgctggaa ggccttctgg aacaggagac cgggctgaat ccaaccgaaa ttatgaccga
tacggcaggt gccagcgatc ttgtctttgg ccttttctgg ctgctgggat accagttttc
tccacgcctg gctgatgccg gtgcttcggt tttctggcga atggaccatg atgccgacta
tggcgtgctg aatgatattg ccagagggca atcagatccc cgaaaaatag tccttcagtg
ggacgaaatg atccggaccg caggctccct gaagctgggc aaagtacagg cctcagtgct
ggtccgttca ttgctgaaaa gtgaacgtcc ctccggactg actcaggcaa tcattgaagt
ggggcgcatc aacaaaacgc tgtatctgct taattatatt gatgatgaag attaccgccg
gcgcattctg acccagctta atcggggaga aagccgtcat gcagttgcca gagccatctg
tcacggtcaa aaaggtgaga taagaaaacg atataccgac ggtcaggaag atcagttggg
agctctgggg ctggtcacta acgccgtcgt gttatggaac actatttata tgcaggcagc
tctggatcat ctccgggcgc agggtgaaac actgaatgat gaagatatcg cacggctctc
cccgctttgc cacggacata tcaatatgct cggccattat tccttcacgc tggcagaact
ggtgaccaaa ggtcatctga gaccattaaa agaggcgtca gaggcagaaa acgttgctta
acgtgagttt tcgttccact gagcgtcaga cccc
//