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ID RSF1010 preliminary; circular DNA; PRO; 8684 BP.
XX
AC M28829;
XX
DT 01-DEC-1994 (Rel. 10, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector RSF1010 - complete.
XX
KW cloning vector.
XX
OS Escherichia coli
OC Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
OC Facultatively anaerobic rods; Enterobacteriaceae; Escherichia.
XX
RN [1]
RC from pBR322 & RSF1010, E.coli mobilization genes mobA/mobB/mobC
RC pED350 from pED825 & RSF1010
RC pED400, pED402, pED404, pED406, pED408, pED410 from pED350 & Tn1725
RC pED412 from pED350 & Tn1725
RC pED401 from pED400
RC pED403 from pED402
RC pED405 from pED404
RC pED407 from pED406
RC pED409 from pED408
RC pED411 from pED410
RC pED359 from pED350
RC pED140, pED141, pED142, pED143, pED144 from pED350 & linker
RC pED145, pED146 from pED350 & linker
RA Derbyshire K.M., Hatfull G., Willetts N.;
RT "Mobilization of the non-conjugative plasmid RSF1010: A genetic and
RT DNA sequence analysis of the mobilization region";
RL Mol. Gen. Genet. 206:161-168(1987).
XX
RN [2]
RC RSF1010-1, RSF1010-2, RSF1010-3, RSF1010-4 from RSF1010
RC RSF1010-5, RSF1010-6 from RSF1010
RC pGC702 from RSF1010-2 & pGC1, TnLac or Tn951
RC pGC704 from RSF1010-4 & pGC1, TnLac or Tn951
RC pGC712 from pGC702
RC pGC714 from pGC704
RC pGC901 from pMR5 & pGC1, TnLac or Tn951
RC pGC811, pGC823 from pPM103 & pGC1, TnLac or Tn951
RA Cornelis G., Sommer H., Saedler H.;
RT "Transposon Tn951 (TnLac) is defective and related to Tn3";
RL Mol. Gen. Genet. 184:241-248(1981).
XX
RN [3]
RC pVH1 from pKT101 & placIq
RC pVH2 from RSF1010 & ptac12
RC pVH3 from RSF1010 & pKK223-3
RC pVH3' from RSF1010 & pKK223-3
RC pVH4 from RSF1010 & ptac12
RC pVH5 from RSF1010 & ptac12
RA Haring V., Scholz P., Scherzinger E., Frey J., Derbyshire K.,
RA Hatfull G.F., Willetts N.S., Bagdasarian M.M.;
RT "Protein repC is involved in copy number control of the broad host
RT range plasmid RSF1010";
RL Proc. Natl. Acad. Sci. U.S.A. 82:6090-6094(1985).
XX
RN [4]
RC from M13deltalac110 & RSF1010, replication origin oriV
RA Honda Y., Sakai H., Komano T.;
RT "Two single-strand DNA initiation signals located in the oriV region
RT of plasmid RSF1010";
RL Gene 68:221-228(1988).
XX
RN [5]
RC RSF1010delta13, RSF1010delta18, RSF1010delta20 from pKT260
RA Frey J., Bagdasarian M.M., Bagdasarian M.;
RT "Replication and copy number control of the broad-host-range plasmid
RT RSF1010";
RL Gene 113:101-106(1992).
XX
RN [6]
RC FSF-1 or F factor from E.coli
RC FSF-2 or F lac from E.coli
RC PSF-2 or ColIb-P9 from E.coli
RC PSF-3 or K88 from E.coli
RC PSF-4 or EntA from E.coli
RC PSF-5 or EntB from E.coli
RC PSF-6 or Hly from E.coli
RC PSF-7 or delta47 from E.coli
RC RSF-8 from S.panama
RC RSF-9 or RTF-1 from E.coli
RC RSF1010 from E. coli incQ plasmid group
RC R6-5 or RSF1020 from S.panama SP219
RA Guerry P., Van Embden J., Falkow S.;
RT "Molecular nature of two non conjugative plasmids carrying drug
RT resistance genes";
RL J. Bacteriol. 117:619-630(1974).
XX
RN [7]
RC RSF1010 or R300B from E. coli incQ plasmids
RC R300B or R1117 from E. coli incQ plasmids
RC pUI106 from R.spaheroides incQ plasmids
RC from RSF1010 & P.putida leu gene
RA Nagahari K., Sakaguchi K.;
RT "RSF1010 plasmid as a potentially useful vector in Pseudomonas
RT species";
RL J. Bacteriol. 133:1527-1529(1978).
XX
RN [8]
RC pMR5 from RP1
RA Robinson M.K., Bennett P.M., Falkow S., Dodd H.M.;
RT "Isolation of a temperature-sensitive derivative of RP1";
RL Plasmid 3:343-347(1980).
XX
RN [9]
RC pGC1 from Yersinia enterocolitica, TnLac or Tn951 lac gene
RA Cornelis G., Bennett P.M., Grinsted J.;
RT "Properties of pGC1, a Lac plasmid originating in Yersinia
RT enterocolitica 842";
RL J. Bacteriol. 127:1058-1062(1976).
XX
RN [10]
RC pPM103 from pSC101
RA Meacock P.A., Cohen S.N.;
RT "Genetic analysis of the interrelationship between plasmid
RT replication and incompatibility";
RL Mol. Gen. Genet. 174:135-147(1979).
XX
RN [11]
RC pUB781 from ColE1 & Tn501
RA Bennett P.M., Grinsted J., Choi C.L., Richmond M.H.;
RT "Characterization of Tn501, a transposon determining resistance
RT to mercuric ions";
RL Mol. Gen. Genet. 159:101-106(1978).
XX
RN [12]
RC [pJOE100 from pJOE series]
RC pJOE105 from pJOE100 & Tn1721
RA Schoffl F., Arnold W., Puhler A., Altenbuchner J., Schmitt R.;
RT "The tetracycline resistance transposons Tn1721 and Tn1771 have
RT three 38-base-pair repeats and generate five-base-pair direct
RT repeats";
RL Mol. Gen. Genet. 181:87-94(1981).
XX
RN [13]
RC RP1-tet from RP1 & tet gene
RC pGC530 from RP1-tet & pGC1, Tn951
RA Cornelis G., Ghosal D., Saedler H.;
RT "Multiple integration sites for the lactose transposon Tn951 on
RT plasmid RP1 and establishment of a coordinate system for Tn951";
RL Mol. Gen. Genet. 168:61-67(1979).
XX
RN [14]
RC pED825 from amp gene
RA Everett R., Willetts N.S.;
RT "Cloning, mutation and location of the F origin of conjugal transfer";
RL EMBO J. 1:747-753(1982).
XX
RN [15]
RC M13deltalac110 from M13mp11 & M13deltaE101
RA Nomura N.;
RT ;
RL Unpublished (1988).
XX
RN [16]
RC M13deltaE101 from M13
RA Kim M.H., Hines J.C., Ray D.S.;
RT "Viable deletions of the M13 complementary strand origin";
RL Proc. Natl. Acad. Sci. U.S.A. 78:6784-6788(1981).
XX
RN [17]
RC RSF1010 or R300B from E. coli incQ plasmids
RC R300B or R1117 from E. coli incQ plasmids
RC pUI106 from R.spaheroides incQ plasmids
RA Scholz P., Haring V., Wittmann-Liebold B., Ashman K.,
RA Bagdasarian M., Scherzinger E.;
RT "Complete nucleotide sequence and gene organization of the
RT broad-host-range plasmid RSF1010";
RL Gene 75:271-288(1989).
XX
CC NM (RSF1010)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR (HpaI site)
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. E. coli, incQ plasmids
FT -> RSF1010 8684bp"
FT CDS 63..866
FT /note="GEN Sm resistance protein A; NCBI gi: 152578"
FT CDS 866..1702
FT /note="GEN Sm resistance protein B; NCBI gi: 152579"
FT CDS complement(2767..3051)
FT /note="GEN mobilization protein C; NCBI gi: 152580"
FT CDS 3250..5379
FT /note="GEN mobilization protein A; NCBI gi: 152581"
FT CDS 3998..4411
FT /note="GEN mobilization protein B; NCBI gi: 152582"
FT CDS 4408..5379
FT /note="GEN replication protein B; NCBI gi: 152583"
FT CDS 5440..5652
FT /note="GEN unknown protein E; NCBI gi: 152584"
FT CDS 5654..5860
FT /note="GEN putative repressor protein F;
FT NCBI gi: 152585"
FT CDS 5890..6729
FT /note="GEN replication protein A; NCBI gi: 152586"
FT CDS 6716..7567
FT /note="GEN replication protein C; NCBI gi: 152587"
FT CDS 7875..8663
FT /note="GEN Su resistance protein; NCBI gi: 152588"
FT rep_origin 0..0
FT /note="ORI E. coli RSF1010"
XX
SQ Sequence 8684 BP; 1757 A; 2627 C; 2667 G; 1633 T; 0 other;
aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg aaggaacctc
cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg cctgtcagag
gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg
cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt tggctcaaag
gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag ggtgcatgct
tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg gatttgctca
aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg gttgatcaat
gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca
atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgctg cacgatcttt
tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc gatatggttg
tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca
cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca
tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg
tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc
gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg ctcattggca
cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga aagtttcatt
gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca ttgctgatga
actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc ggttgctcgg
tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct ctcacatcgt
tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg cgaagctgta
tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc gctttgcagc
tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc
ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga
tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg
tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga
cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct
ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc
ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca
ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca
atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc cttcccgcaa
acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg acgcatgggt
tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg ccgcgcccca
cagctcgggc agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa
gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag aaattcttca
aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag cgcagcgaat
gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg gtggctctgg
taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg catgttccgc
gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc
agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc ccgtactaac
tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac gaacccctgc
aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg cggggtgttg
gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc agcgcccttg
tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct ggtctattcg
ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg ggcaaagccc
gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc gctttagcgg
cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc tgtctcggtc
gatcattcag cccggctcat ccttctggcg tggcggcaga ccgaacaagg cgcggtcgtg
gtcgcgttca aggtacgcat ccattgccgc catgagccga tcctccggcc actcgctgct
gttcaccttg gccaaaatca tggcccccac cagcaccttg cgccttgttt cgttcttgcg
ctcttgctgc tgttcccttg cccgcacccg ctgaatttcg gcattgattc gcgctcgttg
ttcttcgagc ttggccagcc gatccgccgc cttgttgctc cccttaacca tcttgacacc
ccattgttaa tgtgctgtct cgtaggctat catggaggca cagcggcggc aatcccgacc
ctactttgta ggggagggcg cacttaccgg tttctcttcg agaaactggc ctaacggcca
cccttcgggc ggtgcgctct ccgagggcca ttgcatggag ccgaaaagca aaagcaacag
cgaggcagca tggcgattta tcaccttacg gcgaaaaccg gcagcaggtc gggcggccaa
tcggccaggg ccaaggccga ctacatccag cgcgaaggca agtatgcccg cgacatggat
gaagtcttgc acgccgaatc cgggcacatg ccggagttcg tcgagcggcc cgccgactac
tgggatgctg ccgacctgta tgaacgcgcc aatgggcggc tgttcaagga ggtcgaattt
gccctgccgg tcgagctgac cctcgaccag cagaaggcgc tggcgtccga gttcgcccag
cacctgaccg gtgccgagcg cctgccgtat acgctggcca tccatgccgg tggcggcgag
aacccgcact gccacctgat gatctccgag cggatcaatg acggcatcga gcggcccgcc
gctcagtggt tcaagcggta caacggcaag accccggaga agggcggggc acagaagacc
gaagcgctca agcccaaggc atggcttgag cagacccgcg aggcatgggc cgaccatgcc
aaccgggcat tagagcgggc tggccacgac gcccgcattg accacagaac acttgaggcg
cagggcatcg agcgcctgcc cggtgttcac ctggggccga acgtggtgga gatggaaggc
cggggcatcc gcaccgaccg ggcagacgtg gccctgaaca tcgacaccgc caacgcccag
atcatcgact tacaggaata ccgggaggca atagaccatg aacgcaatcg acagagtgaa
gaaatccaga ggcatcaacg agttagcgga gcagatcgaa ccgctggccc agagcatggc
gacactggcc gacgaagccc ggcaggtcat gagccagacc cagcaggcca gcgaggcgca
ggcggcggag tggctgaaag cccagcgcca gacaggggcg gcatgggtgg agctggccaa
agagttgcgg gaggtagccg ccgaggtgag cagcgccgcg cagagcgccc ggagcgcgtc
gcgggggtgg cactggaagc tatggctaac cgtgatgctg gcttccatga tgcctacggt
ggtgctgctg atcgcatcgt tgctcttgct cgacctgacg ccactgacaa ccgaggacgg
ctcgatctgg ctgcgcttgg tggcccgatg aagaacgaca ggactttgca ggccataggc
cgacagctca aggccatggg ctgtgagcgc ttcgatatcg gcgtcaggga cgccaccacc
ggccagatga tgaaccggga atggtcagcc gccgaagtgc tccagaacac gccatggctc
aagcggatga atgcccaggg caatgacgtg tatatcaggc ccgccgagca ggagcggcat
ggtctggtgc tggtggacga cctcagcgag tttgacctgg atgacatgaa agccgagggc
cgggagcctg ccctggtagt ggaaaccagc ccgaagaact atcaggcatg ggtcaaggtg
gccgacgccg caggcggtga acttcggggg cagattgccc ggacgctggc cagcgagtac
gacgccgacc cggccagcgc cgacagccgc cactatggcc gcttggcggg cttcaccaac
cgcaaggaca agcacaccac ccgcgccggt tatcagccgt gggtgctgct gcgtgaatcc
aagggcaaga ccgccaccgc tggcccggcg ctggtgcagc aggctggcca gcagatcgag
caggcccagc ggcagcagga gaaggcccgc aggctggcca gcctcgaact gcccgagcgg
cagcttagcc gccaccggcg cacggcgctg gacgagtacc gcagcgagat ggccgggctg
gtcaagcgct tcggtgatga cctcagcaag tgcgacttta tcgccgcgca gaagctggcc
agccggggcc gcagtgccga ggaaatcggc aaggccatgg ccgaggccag cccagcgctg
gcagagcgca agcccggcca cgaagcggat tacatcgagc gcaccgtcag caaggtcatg
ggtctgccca gcgtccagct tgcgcgggcc gagctggcac gggcaccggc accccgccag
cgaggcatgg acaggggcgg gccagatttc agcatgtagt gcttgcgttg gtactcacgc
ctgttatact atgagtactc acgcacagaa gggggtttta tggaatacga aaaaagcgct
tcagggtcgg tctacctgat caaaagtgac aagggctatt ggttgcccgg tggctttggt
tatacgtcaa acaaggccga ggctggccgc ttttcagtcg ctgatatggc cagccttaac
cttgacggct gcaccttgtc cttgttccgc gaagacaagc ctttcggccc cggcaagttt
ctcggtgact gatatgaaag accaaaagga caagcagacc ggcgacctgc tggccagccc
tgacgctgta cgccaagcgc gatatgccga gcgcatgaag gccaaaggga tgcgtcagcg
caagttctgg ctgaccgacg acgaatacga ggcgctgcgc gagtgcctgg aagaactcag
agcggcgcag ggcgggggta gtgaccccgc cagcgcctaa ccaccaactg cctgcaaagg
aggcaatcaa tggctaccca taagcctatc aatattctgg aggcgttcgc agcagcgccg
ccaccgctgg actacgtttt gcccaacatg gtggccggta cggtcggggc gctggtgtcg
cccggtggtg ccggtaaatc catgctggcc ctgcaactgg ccgcacagat tgcaggcggg
ccggatctgc tggaggtggg cgaactgccc accggcccgg tgatctacct gcccgccgaa
gacccgccca ccgccattca tcaccgcctg cacgcccttg gggcgcacct cagcgccgag
gaacggcaag ccgtggctga cggcctgctg atccagccgc tgatcggcag cctgcccaac
atcatggccc cggagtggtt cgacggcctc aagcgcgccg ccgagggccg ccgcctgatg
gtgctggaca cgctgcgccg gttccacatc gaggaagaaa acgccagcgg ccccatggcc
caggtcatcg gtcgcatgga ggccatcgcc gccgataccg ggtgctctat cgtgttcctg
caccatgcca gcaagggcgc ggccatgatg ggcgcaggcg accagcagca ggccagccgg
ggcagctcgg tactggtcga taacatccgc tggcagtcct acctgtcgag catgaccagc
gccgaggccg aggaatgggg tgtggacgac gaccagcgcc ggttcttcgt ccgcttcggt
gtgagcaagg ccaactatgg cgcaccgttc gctgatcggt ggttcaggcg gcatgacggc
ggggtgctca agcccgccgt gctggagagg cagcgcaaga gcaagggggt gccccgtggt
gaagcctaag aacaagcaca gcctcagcca cgtccggcac gacccggcgc actgtctggc
ccccggcctg ttccgtgccc tcaagcgggg cgagcgcaag cgcagcaagc tggacgtgac
gtatgactac ggcgacggca agcggatcga gttcagcggc ccggagccgc tgggcgctga
tgatctgcgc atcctgcaag ggctggtggc catggctggg cctaatggcc tagtgcttgg
cccggaaccc aagaccgaag gcggacggca gctccggctg ttcctggaac ccaagtggga
ggccgtcacc gctgaatgcc atgtggtcaa aggtagctat cgggcgctgg caaaggaaat
cggggcagag gtcgatagtg gtggggcgct caagcacata caggactgca tcgagcgcct
ttggaaggta tccatcatcg cccagaatgg ccgcaagcgg caggggtttc ggctgctgtc
ggagtacgcc agcgacgagg cggacgggcg cctgtacgtg gccctgaacc ccttgatcgc
gcaggccgtc atgggtggcg gccagcatgt gcgcatcagc atggacgagg tgcgggcgct
ggacagcgaa accgcccgcc tgctgcacca gcggctgtgt ggctggatcg accccggcaa
aaccggcaag gcttccatag ataccttgtg cggctatgtc tggccgtcag aggccagtgg
ttcgaccatg cgcaagcgcc gccagcgggt gcgcgaggcg ttgccggagc tggtcgcgct
gggctggacg gtaaccgagt tcgcggcggg caagtacgac atcacccggc ccaaggcggc
aggctgaccc cccccactct attgtaaaca agacattttt atcttttata ttcaatggct
tattttcctg ctaattggta ataccatgaa aaataccatg ctcagaaaag gcttaacaat
attttgaaaa attgcctact gagcgctgcc gcacagctcc ataggccgct ttcctggctt
tgcttccaga tgtatgctct tctgctcctg cagctaatgg atcaccgcaa acaggttact
cgcctgggga ttccctttcg acccgagcat ccgtatgata ctcatgctcg attattatta
ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac ataacctcgg
acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg caggcgcgta
agctgatggc cgagggggca gatgtgatcg acctggtccg gcatccagca atcccgacgc
cgcgcctgtt tcgtccgaca cagaaatcgc gcgtatgcgc cggtgctgga cgcgctcagg
cagatggcat tcccgtctcg ctcgacagtt atcaacccgc gacgcaagcc tatgccttgt
cgcgtggtgt ggcctatctc aatgatattc gcggttttcc agacgctgcg ttctatccgc
aattggcgaa atcatctgcc aaactcgtcg ttatgcattc ggtgcaagac gggcaggcag
atcggcgcga ggcacccgct ggcgacatca tggatcacat tgcggcgttc tttgacgcgc
gcatcgcggc gctgacgggt gccggtatca aacgcaaccg ccttgtcctt gatcccggca
tggggttttt tctgggggct gctcccgaaa cctcgctctc ggtgctggcg cggttcgatg
aattgcggct gcgcttcgat ttgccggtgc ttctgtctgt ttcgcgcaaa tcctttctgc
gcgcgctcac aggccgtggt ccgggggtgt cggggccgcg acactcgctg cagagcttgc
cgccgccgca ggtggagctg acttcatccg cacacacgag ccgcgcccct tgcgcgacgg
gctggcggta ttggcggcgc tgaaagaaac cgcaagaatt cgtt
//