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ID   RSF1010    preliminary; circular DNA; PRO; 8684 BP.
XX
AC   M28829;
XX
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector RSF1010 - complete.
XX
KW   cloning vector.
XX
OS   Escherichia coli
OC   Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
OC   Facultatively anaerobic rods; Enterobacteriaceae; Escherichia.
XX
RN   [1]
RC   from pBR322 & RSF1010, E.coli mobilization genes mobA/mobB/mobC
RC   pED350 from pED825 & RSF1010
RC   pED400, pED402, pED404, pED406, pED408, pED410 from pED350 & Tn1725
RC   pED412 from pED350 & Tn1725
RC   pED401 from pED400
RC   pED403 from pED402
RC   pED405 from pED404
RC   pED407 from pED406
RC   pED409 from pED408
RC   pED411 from pED410
RC   pED359 from pED350
RC   pED140, pED141, pED142, pED143, pED144 from pED350 & linker
RC   pED145, pED146 from pED350 & linker
RA   Derbyshire K.M., Hatfull G., Willetts N.;
RT   "Mobilization of the non-conjugative plasmid RSF1010: A genetic and
RT   DNA sequence analysis of the mobilization region";
RL   Mol. Gen. Genet. 206:161-168(1987).
XX
RN   [2]
RC   RSF1010-1, RSF1010-2, RSF1010-3, RSF1010-4 from RSF1010
RC   RSF1010-5, RSF1010-6 from RSF1010
RC   pGC702 from RSF1010-2 & pGC1, TnLac or Tn951
RC   pGC704 from RSF1010-4 & pGC1, TnLac or Tn951
RC   pGC712 from pGC702
RC   pGC714 from pGC704
RC   pGC901 from pMR5 & pGC1, TnLac or Tn951
RC   pGC811, pGC823 from pPM103 & pGC1, TnLac or Tn951
RA   Cornelis G., Sommer H., Saedler H.;
RT   "Transposon Tn951 (TnLac) is defective and related to Tn3";
RL   Mol. Gen. Genet. 184:241-248(1981).
XX
RN   [3]
RC   pVH1 from pKT101 & placIq
RC   pVH2 from RSF1010 & ptac12
RC   pVH3 from RSF1010 & pKK223-3
RC   pVH3' from RSF1010 & pKK223-3
RC   pVH4 from RSF1010 & ptac12
RC   pVH5 from RSF1010 & ptac12
RA   Haring V., Scholz P., Scherzinger E., Frey J., Derbyshire K.,
RA   Hatfull G.F., Willetts N.S., Bagdasarian M.M.;
RT   "Protein repC is involved in copy number control of the broad host
RT   range plasmid RSF1010";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:6090-6094(1985).
XX
RN   [4]
RC   from M13deltalac110 & RSF1010, replication origin oriV
RA   Honda Y., Sakai H., Komano T.;
RT   "Two single-strand DNA initiation signals located in the oriV region
RT   of plasmid RSF1010";
RL   Gene 68:221-228(1988).
XX
RN   [5]
RC   RSF1010delta13, RSF1010delta18, RSF1010delta20 from pKT260
RA   Frey J., Bagdasarian M.M., Bagdasarian M.;
RT   "Replication and copy number control of the broad-host-range plasmid
RT   RSF1010";
RL   Gene 113:101-106(1992).
XX
RN   [6]
RC   FSF-1 or F factor from E.coli
RC   FSF-2 or F lac from E.coli
RC   PSF-2 or ColIb-P9 from E.coli
RC   PSF-3 or K88 from E.coli
RC   PSF-4 or EntA from E.coli
RC   PSF-5 or EntB from E.coli
RC   PSF-6 or Hly from E.coli
RC   PSF-7 or delta47 from E.coli
RC   RSF-8 from S.panama
RC   RSF-9 or RTF-1 from E.coli
RC   RSF1010 from E. coli incQ plasmid group
RC   R6-5 or RSF1020 from S.panama SP219
RA   Guerry P., Van Embden J., Falkow S.;
RT   "Molecular nature of two non conjugative plasmids carrying drug
RT   resistance genes";
RL   J. Bacteriol. 117:619-630(1974).
XX
RN   [7]
RC   RSF1010 or R300B from E. coli incQ plasmids
RC   R300B or R1117 from E. coli incQ plasmids
RC   pUI106 from R.spaheroides incQ plasmids
RC   from RSF1010 & P.putida leu gene
RA   Nagahari K., Sakaguchi K.;
RT   "RSF1010 plasmid as a potentially useful vector in Pseudomonas
RT   species";
RL   J. Bacteriol. 133:1527-1529(1978).
XX
RN   [8]
RC   pMR5 from RP1
RA   Robinson M.K., Bennett P.M., Falkow S., Dodd H.M.;
RT   "Isolation of a temperature-sensitive derivative of RP1";
RL   Plasmid 3:343-347(1980).
XX
RN   [9]
RC   pGC1 from Yersinia enterocolitica, TnLac or Tn951 lac gene
RA   Cornelis G., Bennett P.M., Grinsted J.;
RT   "Properties of pGC1, a Lac plasmid originating in Yersinia
RT   enterocolitica 842";
RL   J. Bacteriol. 127:1058-1062(1976).
XX
RN   [10]
RC   pPM103 from pSC101
RA   Meacock P.A., Cohen S.N.;
RT   "Genetic analysis of the interrelationship between plasmid
RT   replication and incompatibility";
RL   Mol. Gen. Genet. 174:135-147(1979).
XX
RN   [11]
RC   pUB781 from ColE1 & Tn501
RA   Bennett P.M., Grinsted J., Choi C.L., Richmond M.H.;
RT   "Characterization of Tn501, a transposon determining resistance
RT   to mercuric ions";
RL   Mol. Gen. Genet. 159:101-106(1978).
XX
RN   [12]
RC   [pJOE100 from pJOE series]
RC   pJOE105 from pJOE100 & Tn1721
RA   Schoffl F., Arnold W., Puhler A., Altenbuchner J., Schmitt R.;
RT   "The tetracycline resistance transposons Tn1721 and Tn1771 have
RT   three 38-base-pair repeats and generate five-base-pair direct
RT   repeats";
RL   Mol. Gen. Genet. 181:87-94(1981).
XX
RN   [13]
RC   RP1-tet from RP1 & tet gene
RC   pGC530 from RP1-tet & pGC1, Tn951
RA   Cornelis G., Ghosal D., Saedler H.;
RT   "Multiple integration sites for the lactose transposon Tn951 on
RT   plasmid RP1 and establishment of a coordinate system for Tn951";
RL   Mol. Gen. Genet. 168:61-67(1979).
XX
RN   [14]
RC   pED825 from amp gene
RA   Everett R., Willetts N.S.;
RT   "Cloning, mutation and location of the F origin of conjugal transfer";
RL   EMBO J. 1:747-753(1982).
XX
RN   [15]
RC   M13deltalac110 from M13mp11 & M13deltaE101
RA   Nomura N.;
RT   ;
RL   Unpublished (1988).
XX
RN   [16]
RC   M13deltaE101 from M13
RA   Kim M.H., Hines J.C., Ray D.S.;
RT   "Viable deletions of the M13 complementary strand origin";
RL   Proc. Natl. Acad. Sci. U.S.A. 78:6784-6788(1981).
XX
RN   [17]
RC   RSF1010 or R300B from E. coli incQ plasmids
RC   R300B or R1117 from E. coli incQ plasmids
RC   pUI106 from R.spaheroides incQ plasmids
RA   Scholz P., Haring V., Wittmann-Liebold B., Ashman K.,
RA   Bagdasarian M., Scherzinger E.;
RT   "Complete nucleotide sequence and gene organization of the
RT   broad-host-range plasmid RSF1010";
RL   Gene 75:271-288(1989).
XX
CC   NM (RSF1010)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR (HpaI site)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli, incQ plasmids
FT                   -> RSF1010 8684bp"
FT   CDS             63..866
FT                   /note="GEN Sm resistance protein A; NCBI gi: 152578"
FT   CDS             866..1702
FT                   /note="GEN Sm resistance protein B; NCBI gi: 152579"
FT   CDS             complement(2767..3051)
FT                   /note="GEN mobilization protein C; NCBI gi: 152580"
FT   CDS             3250..5379
FT                   /note="GEN mobilization protein A; NCBI gi: 152581"
FT   CDS             3998..4411
FT                   /note="GEN mobilization protein B; NCBI gi: 152582"
FT   CDS             4408..5379
FT                   /note="GEN replication protein B; NCBI gi: 152583"
FT   CDS             5440..5652
FT                   /note="GEN unknown protein E; NCBI gi: 152584"
FT   CDS             5654..5860
FT                   /note="GEN putative repressor protein F;
FT                   NCBI gi: 152585"
FT   CDS             5890..6729
FT                   /note="GEN replication protein A; NCBI gi: 152586"
FT   CDS             6716..7567
FT                   /note="GEN replication protein C; NCBI gi: 152587"
FT   CDS             7875..8663
FT                   /note="GEN Su resistance protein; NCBI gi: 152588"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RSF1010"
XX
SQ   Sequence 8684 BP; 1757 A; 2627 C; 2667 G; 1633 T; 0 other;
     aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg aaggaacctc
     cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg cctgtcagag
     gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg
     cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt tggctcaaag
     gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag ggtgcatgct
     tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg gatttgctca
     aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg gttgatcaat
     gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca
     atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgctg cacgatcttt
     tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc gatatggttg
     tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca
     cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca
     tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg
     tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc
     gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg ctcattggca
     cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga aagtttcatt
     gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca ttgctgatga
     actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc ggttgctcgg
     tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct ctcacatcgt
     tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg cgaagctgta
     tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc gctttgcagc
     tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc
     ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga
     tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg
     tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga
     cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct
     ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc
     ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca
     ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca
     atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc cttcccgcaa
     acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg acgcatgggt
     tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg ccgcgcccca
     cagctcgggc agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa
     gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag aaattcttca
     aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag cgcagcgaat
     gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg gtggctctgg
     taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg catgttccgc
     gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc
     agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc ccgtactaac
     tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac gaacccctgc
     aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg cggggtgttg
     gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc agcgcccttg
     tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct ggtctattcg
     ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg ggcaaagccc
     gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc gctttagcgg
     cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc tgtctcggtc
     gatcattcag cccggctcat ccttctggcg tggcggcaga ccgaacaagg cgcggtcgtg
     gtcgcgttca aggtacgcat ccattgccgc catgagccga tcctccggcc actcgctgct
     gttcaccttg gccaaaatca tggcccccac cagcaccttg cgccttgttt cgttcttgcg
     ctcttgctgc tgttcccttg cccgcacccg ctgaatttcg gcattgattc gcgctcgttg
     ttcttcgagc ttggccagcc gatccgccgc cttgttgctc cccttaacca tcttgacacc
     ccattgttaa tgtgctgtct cgtaggctat catggaggca cagcggcggc aatcccgacc
     ctactttgta ggggagggcg cacttaccgg tttctcttcg agaaactggc ctaacggcca
     cccttcgggc ggtgcgctct ccgagggcca ttgcatggag ccgaaaagca aaagcaacag
     cgaggcagca tggcgattta tcaccttacg gcgaaaaccg gcagcaggtc gggcggccaa
     tcggccaggg ccaaggccga ctacatccag cgcgaaggca agtatgcccg cgacatggat
     gaagtcttgc acgccgaatc cgggcacatg ccggagttcg tcgagcggcc cgccgactac
     tgggatgctg ccgacctgta tgaacgcgcc aatgggcggc tgttcaagga ggtcgaattt
     gccctgccgg tcgagctgac cctcgaccag cagaaggcgc tggcgtccga gttcgcccag
     cacctgaccg gtgccgagcg cctgccgtat acgctggcca tccatgccgg tggcggcgag
     aacccgcact gccacctgat gatctccgag cggatcaatg acggcatcga gcggcccgcc
     gctcagtggt tcaagcggta caacggcaag accccggaga agggcggggc acagaagacc
     gaagcgctca agcccaaggc atggcttgag cagacccgcg aggcatgggc cgaccatgcc
     aaccgggcat tagagcgggc tggccacgac gcccgcattg accacagaac acttgaggcg
     cagggcatcg agcgcctgcc cggtgttcac ctggggccga acgtggtgga gatggaaggc
     cggggcatcc gcaccgaccg ggcagacgtg gccctgaaca tcgacaccgc caacgcccag
     atcatcgact tacaggaata ccgggaggca atagaccatg aacgcaatcg acagagtgaa
     gaaatccaga ggcatcaacg agttagcgga gcagatcgaa ccgctggccc agagcatggc
     gacactggcc gacgaagccc ggcaggtcat gagccagacc cagcaggcca gcgaggcgca
     ggcggcggag tggctgaaag cccagcgcca gacaggggcg gcatgggtgg agctggccaa
     agagttgcgg gaggtagccg ccgaggtgag cagcgccgcg cagagcgccc ggagcgcgtc
     gcgggggtgg cactggaagc tatggctaac cgtgatgctg gcttccatga tgcctacggt
     ggtgctgctg atcgcatcgt tgctcttgct cgacctgacg ccactgacaa ccgaggacgg
     ctcgatctgg ctgcgcttgg tggcccgatg aagaacgaca ggactttgca ggccataggc
     cgacagctca aggccatggg ctgtgagcgc ttcgatatcg gcgtcaggga cgccaccacc
     ggccagatga tgaaccggga atggtcagcc gccgaagtgc tccagaacac gccatggctc
     aagcggatga atgcccaggg caatgacgtg tatatcaggc ccgccgagca ggagcggcat
     ggtctggtgc tggtggacga cctcagcgag tttgacctgg atgacatgaa agccgagggc
     cgggagcctg ccctggtagt ggaaaccagc ccgaagaact atcaggcatg ggtcaaggtg
     gccgacgccg caggcggtga acttcggggg cagattgccc ggacgctggc cagcgagtac
     gacgccgacc cggccagcgc cgacagccgc cactatggcc gcttggcggg cttcaccaac
     cgcaaggaca agcacaccac ccgcgccggt tatcagccgt gggtgctgct gcgtgaatcc
     aagggcaaga ccgccaccgc tggcccggcg ctggtgcagc aggctggcca gcagatcgag
     caggcccagc ggcagcagga gaaggcccgc aggctggcca gcctcgaact gcccgagcgg
     cagcttagcc gccaccggcg cacggcgctg gacgagtacc gcagcgagat ggccgggctg
     gtcaagcgct tcggtgatga cctcagcaag tgcgacttta tcgccgcgca gaagctggcc
     agccggggcc gcagtgccga ggaaatcggc aaggccatgg ccgaggccag cccagcgctg
     gcagagcgca agcccggcca cgaagcggat tacatcgagc gcaccgtcag caaggtcatg
     ggtctgccca gcgtccagct tgcgcgggcc gagctggcac gggcaccggc accccgccag
     cgaggcatgg acaggggcgg gccagatttc agcatgtagt gcttgcgttg gtactcacgc
     ctgttatact atgagtactc acgcacagaa gggggtttta tggaatacga aaaaagcgct
     tcagggtcgg tctacctgat caaaagtgac aagggctatt ggttgcccgg tggctttggt
     tatacgtcaa acaaggccga ggctggccgc ttttcagtcg ctgatatggc cagccttaac
     cttgacggct gcaccttgtc cttgttccgc gaagacaagc ctttcggccc cggcaagttt
     ctcggtgact gatatgaaag accaaaagga caagcagacc ggcgacctgc tggccagccc
     tgacgctgta cgccaagcgc gatatgccga gcgcatgaag gccaaaggga tgcgtcagcg
     caagttctgg ctgaccgacg acgaatacga ggcgctgcgc gagtgcctgg aagaactcag
     agcggcgcag ggcgggggta gtgaccccgc cagcgcctaa ccaccaactg cctgcaaagg
     aggcaatcaa tggctaccca taagcctatc aatattctgg aggcgttcgc agcagcgccg
     ccaccgctgg actacgtttt gcccaacatg gtggccggta cggtcggggc gctggtgtcg
     cccggtggtg ccggtaaatc catgctggcc ctgcaactgg ccgcacagat tgcaggcggg
     ccggatctgc tggaggtggg cgaactgccc accggcccgg tgatctacct gcccgccgaa
     gacccgccca ccgccattca tcaccgcctg cacgcccttg gggcgcacct cagcgccgag
     gaacggcaag ccgtggctga cggcctgctg atccagccgc tgatcggcag cctgcccaac
     atcatggccc cggagtggtt cgacggcctc aagcgcgccg ccgagggccg ccgcctgatg
     gtgctggaca cgctgcgccg gttccacatc gaggaagaaa acgccagcgg ccccatggcc
     caggtcatcg gtcgcatgga ggccatcgcc gccgataccg ggtgctctat cgtgttcctg
     caccatgcca gcaagggcgc ggccatgatg ggcgcaggcg accagcagca ggccagccgg
     ggcagctcgg tactggtcga taacatccgc tggcagtcct acctgtcgag catgaccagc
     gccgaggccg aggaatgggg tgtggacgac gaccagcgcc ggttcttcgt ccgcttcggt
     gtgagcaagg ccaactatgg cgcaccgttc gctgatcggt ggttcaggcg gcatgacggc
     ggggtgctca agcccgccgt gctggagagg cagcgcaaga gcaagggggt gccccgtggt
     gaagcctaag aacaagcaca gcctcagcca cgtccggcac gacccggcgc actgtctggc
     ccccggcctg ttccgtgccc tcaagcgggg cgagcgcaag cgcagcaagc tggacgtgac
     gtatgactac ggcgacggca agcggatcga gttcagcggc ccggagccgc tgggcgctga
     tgatctgcgc atcctgcaag ggctggtggc catggctggg cctaatggcc tagtgcttgg
     cccggaaccc aagaccgaag gcggacggca gctccggctg ttcctggaac ccaagtggga
     ggccgtcacc gctgaatgcc atgtggtcaa aggtagctat cgggcgctgg caaaggaaat
     cggggcagag gtcgatagtg gtggggcgct caagcacata caggactgca tcgagcgcct
     ttggaaggta tccatcatcg cccagaatgg ccgcaagcgg caggggtttc ggctgctgtc
     ggagtacgcc agcgacgagg cggacgggcg cctgtacgtg gccctgaacc ccttgatcgc
     gcaggccgtc atgggtggcg gccagcatgt gcgcatcagc atggacgagg tgcgggcgct
     ggacagcgaa accgcccgcc tgctgcacca gcggctgtgt ggctggatcg accccggcaa
     aaccggcaag gcttccatag ataccttgtg cggctatgtc tggccgtcag aggccagtgg
     ttcgaccatg cgcaagcgcc gccagcgggt gcgcgaggcg ttgccggagc tggtcgcgct
     gggctggacg gtaaccgagt tcgcggcggg caagtacgac atcacccggc ccaaggcggc
     aggctgaccc cccccactct attgtaaaca agacattttt atcttttata ttcaatggct
     tattttcctg ctaattggta ataccatgaa aaataccatg ctcagaaaag gcttaacaat
     attttgaaaa attgcctact gagcgctgcc gcacagctcc ataggccgct ttcctggctt
     tgcttccaga tgtatgctct tctgctcctg cagctaatgg atcaccgcaa acaggttact
     cgcctgggga ttccctttcg acccgagcat ccgtatgata ctcatgctcg attattatta
     ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac ataacctcgg
     acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg caggcgcgta
     agctgatggc cgagggggca gatgtgatcg acctggtccg gcatccagca atcccgacgc
     cgcgcctgtt tcgtccgaca cagaaatcgc gcgtatgcgc cggtgctgga cgcgctcagg
     cagatggcat tcccgtctcg ctcgacagtt atcaacccgc gacgcaagcc tatgccttgt
     cgcgtggtgt ggcctatctc aatgatattc gcggttttcc agacgctgcg ttctatccgc
     aattggcgaa atcatctgcc aaactcgtcg ttatgcattc ggtgcaagac gggcaggcag
     atcggcgcga ggcacccgct ggcgacatca tggatcacat tgcggcgttc tttgacgcgc
     gcatcgcggc gctgacgggt gccggtatca aacgcaaccg ccttgtcctt gatcccggca
     tggggttttt tctgggggct gctcccgaaa cctcgctctc ggtgctggcg cggttcgatg
     aattgcggct gcgcttcgat ttgccggtgc ttctgtctgt ttcgcgcaaa tcctttctgc
     gcgcgctcac aggccgtggt ccgggggtgt cggggccgcg acactcgctg cagagcttgc
     cgccgccgca ggtggagctg acttcatccg cacacacgag ccgcgcccct tgcgcgacgg
     gctggcggta ttggcggcgc tgaaagaaac cgcaagaatt cgtt
//