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ID   RSF1010KMR preliminary; circular DNA; SYN; 9227 BP.
XX
AC   IG9884;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Broad host range/E.coli plasmid vector RSF1010KmR - KmR-cos region.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pJRD185 from pJRD184
RC   pJRD186 from pIC20R & pJRD185
RC   pEMBLcos from pEMBL8 & MUA-10, lambda cos
RC   RSF1010KmR or RSF1010Kan from RSF1010 & pKC7
RC   pJRD203 from RSF1010KmR or RSF1010Kan & pEMBLcos
RC   pJRD204 from pJRD203
RC   pJRD209 from pJRD203
RC   pJRD210 from pJRD204
RC   pJRD212 from pJRD209 & pJRD210
RC   pJRD215 from pJRD212 & pJRD186
RA   Davison J., Heusterspreute M., Chevalier N., Ha-Thi V., Brunel F.;
RT   "Vectors with restriction site banks: V. pPJRD215, a wide-host-
RT   range cosmid vector with multiple cloning sites";
RL   Gene 51:275-280(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (RSF1010KmR)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli MM294)(E.coli)(broad host range)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (RSF1010)(pKC7)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
XX
FT   misc_feature    0..0
FT                   /note="1. RSF1010 remove EcoRI-PstI 904bp 7773..8677,
FT                   \ sulf/7780bp
FT                   2. pKC7 EcoRI-PstI 1447bp 5834..5835..1446, Tn5 kan
FT                   -> RSF1010KmR 9227bp"
FT   -               1..7772
FT                   /note="RSF1010 1..7772 7772bp
FT                   EcoRI = G^AATTC"
FT   -               7773..9219
FT                   /note="pKC7 5834..5835..1445 1447bp
FT                   PstI = CTGCA^G"
FT   -               9220..9227
FT                   /note="RSF1010 8677..8684 8bp"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan);
FT                   fragment; from pKC7"
FT   CDS             0..0
FT                   /note="ANT E. coli streptomycin resistance gene (str);
FT                   fragment; from pKC7"
FT   rep_origin      0..0
FT                   /note="ORI E. coli IncQ plasmid RSF1010 oriT and oriV"
XX
SQ   Sequence 9227 BP; 1861 A; 2786 C; 2828 G; 1752 T; 0 other;
     aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg aaggaacctc
     cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg cctgtcagag
     gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg
     cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt tggctcaaag
     gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag ggtgcatgct
     tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg gatttgctca
     aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg gttgatcaat
     gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca
     atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgctg cacgatcttt
     tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc gatatggttg
     tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca
     cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca
     tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg
     tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc
     gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg ctcattggca
     cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga aagtttcatt
     gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca ttgctgatga
     actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc ggttgctcgg
     tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct ctcacatcgt
     tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg cgaagctgta
     tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc gctttgcagc
     tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc
     ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga
     tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg
     tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga
     cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct
     ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc
     ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca
     ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca
     atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc cttcccgcaa
     acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg acgcatgggt
     tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg ccgcgcccca
     cagctcgggc agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa
     gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag aaattcttca
     aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag cgcagcgaat
     gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg gtggctctgg
     taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg catgttccgc
     gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc
     agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc ccgtactaac
     tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac gaacccctgc
     aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg cggggtgttg
     gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc agcgcccttg
     tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct ggtctattcg
     ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg ggcaaagccc
     gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc gctttagcgg
     cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc tgtctcggtc
     gatcattcag cccggctcat ccttctggcg tggcggcaga ccgaacaagg cgcggtcgtg
     gtcgcgttca aggtacgcat ccattgccgc catgagccga tcctccggcc actcgctgct
     gttcaccttg gccaaaatca tggcccccac cagcaccttg cgccttgttt cgttcttgcg
     ctcttgctgc tgttcccttg cccgcacccg ctgaatttcg gcattgattc gcgctcgttg
     ttcttcgagc ttggccagcc gatccgccgc cttgttgctc cccttaacca tcttgacacc
     ccattgttaa tgtgctgtct cgtaggctat catggaggca cagcggcggc aatcccgacc
     ctactttgta ggggagggcg cacttaccgg tttctcttcg agaaactggc ctaacggcca
     cccttcgggc ggtgcgctct ccgagggcca ttgcatggag ccgaaaagca aaagcaacag
     cgaggcagca tggcgattta tcaccttacg gcgaaaaccg gcagcaggtc gggcggccaa
     tcggccaggg ccaaggccga ctacatccag cgcgaaggca agtatgcccg cgacatggat
     gaagtcttgc acgccgaatc cgggcacatg ccggagttcg tcgagcggcc cgccgactac
     tgggatgctg ccgacctgta tgaacgcgcc aatgggcggc tgttcaagga ggtcgaattt
     gccctgccgg tcgagctgac cctcgaccag cagaaggcgc tggcgtccga gttcgcccag
     cacctgaccg gtgccgagcg cctgccgtat acgctggcca tccatgccgg tggcggcgag
     aacccgcact gccacctgat gatctccgag cggatcaatg acggcatcga gcggcccgcc
     gctcagtggt tcaagcggta caacggcaag accccggaga agggcggggc acagaagacc
     gaagcgctca agcccaaggc atggcttgag cagacccgcg aggcatgggc cgaccatgcc
     aaccgggcat tagagcgggc tggccacgac gcccgcattg accacagaac acttgaggcg
     cagggcatcg agcgcctgcc cggtgttcac ctggggccga acgtggtgga gatggaaggc
     cggggcatcc gcaccgaccg ggcagacgtg gccctgaaca tcgacaccgc caacgcccag
     atcatcgact tacaggaata ccgggaggca atagaccatg aacgcaatcg acagagtgaa
     gaaatccaga ggcatcaacg agttagcgga gcagatcgaa ccgctggccc agagcatggc
     gacactggcc gacgaagccc ggcaggtcat gagccagacc cagcaggcca gcgaggcgca
     ggcggcggag tggctgaaag cccagcgcca gacaggggcg gcatgggtgg agctggccaa
     agagttgcgg gaggtagccg ccgaggtgag cagcgccgcg cagagcgccc ggagcgcgtc
     gcgggggtgg cactggaagc tatggctaac cgtgatgctg gcttccatga tgcctacggt
     ggtgctgctg atcgcatcgt tgctcttgct cgacctgacg ccactgacaa ccgaggacgg
     ctcgatctgg ctgcgcttgg tggcccgatg aagaacgaca ggactttgca ggccataggc
     cgacagctca aggccatggg ctgtgagcgc ttcgatatcg gcgtcaggga cgccaccacc
     ggccagatga tgaaccggga atggtcagcc gccgaagtgc tccagaacac gccatggctc
     aagcggatga atgcccaggg caatgacgtg tatatcaggc ccgccgagca ggagcggcat
     ggtctggtgc tggtggacga cctcagcgag tttgacctgg atgacatgaa agccgagggc
     cgggagcctg ccctggtagt ggaaaccagc ccgaagaact atcaggcatg ggtcaaggtg
     gccgacgccg caggcggtga acttcggggg cagattgccc ggacgctggc cagcgagtac
     gacgccgacc cggccagcgc cgacagccgc cactatggcc gcttggcggg cttcaccaac
     cgcaaggaca agcacaccac ccgcgccggt tatcagccgt gggtgctgct gcgtgaatcc
     aagggcaaga ccgccaccgc tggcccggcg ctggtgcagc aggctggcca gcagatcgag
     caggcccagc ggcagcagga gaaggcccgc aggctggcca gcctcgaact gcccgagcgg
     cagcttagcc gccaccggcg cacggcgctg gacgagtacc gcagcgagat ggccgggctg
     gtcaagcgct tcggtgatga cctcagcaag tgcgacttta tcgccgcgca gaagctggcc
     agccggggcc gcagtgccga ggaaatcggc aaggccatgg ccgaggccag cccagcgctg
     gcagagcgca agcccggcca cgaagcggat tacatcgagc gcaccgtcag caaggtcatg
     ggtctgccca gcgtccagct tgcgcgggcc gagctggcac gggcaccggc accccgccag
     cgaggcatgg acaggggcgg gccagatttc agcatgtagt gcttgcgttg gtactcacgc
     ctgttatact atgagtactc acgcacagaa gggggtttta tggaatacga aaaaagcgct
     tcagggtcgg tctacctgat caaaagtgac aagggctatt ggttgcccgg tggctttggt
     tatacgtcaa acaaggccga ggctggccgc ttttcagtcg ctgatatggc cagccttaac
     cttgacggct gcaccttgtc cttgttccgc gaagacaagc ctttcggccc cggcaagttt
     ctcggtgact gatatgaaag accaaaagga caagcagacc ggcgacctgc tggccagccc
     tgacgctgta cgccaagcgc gatatgccga gcgcatgaag gccaaaggga tgcgtcagcg
     caagttctgg ctgaccgacg acgaatacga ggcgctgcgc gagtgcctgg aagaactcag
     agcggcgcag ggcgggggta gtgaccccgc cagcgcctaa ccaccaactg cctgcaaagg
     aggcaatcaa tggctaccca taagcctatc aatattctgg aggcgttcgc agcagcgccg
     ccaccgctgg actacgtttt gcccaacatg gtggccggta cggtcggggc gctggtgtcg
     cccggtggtg ccggtaaatc catgctggcc ctgcaactgg ccgcacagat tgcaggcggg
     ccggatctgc tggaggtggg cgaactgccc accggcccgg tgatctacct gcccgccgaa
     gacccgccca ccgccattca tcaccgcctg cacgcccttg gggcgcacct cagcgccgag
     gaacggcaag ccgtggctga cggcctgctg atccagccgc tgatcggcag cctgcccaac
     atcatggccc cggagtggtt cgacggcctc aagcgcgccg ccgagggccg ccgcctgatg
     gtgctggaca cgctgcgccg gttccacatc gaggaagaaa acgccagcgg ccccatggcc
     caggtcatcg gtcgcatgga ggccatcgcc gccgataccg ggtgctctat cgtgttcctg
     caccatgcca gcaagggcgc ggccatgatg ggcgcaggcg accagcagca ggccagccgg
     ggcagctcgg tactggtcga taacatccgc tggcagtcct acctgtcgag catgaccagc
     gccgaggccg aggaatgggg tgtggacgac gaccagcgcc ggttcttcgt ccgcttcggt
     gtgagcaagg ccaactatgg cgcaccgttc gctgatcggt ggttcaggcg gcatgacggc
     ggggtgctca agcccgccgt gctggagagg cagcgcaaga gcaagggggt gccccgtggt
     gaagcctaag aacaagcaca gcctcagcca cgtccggcac gacccggcgc actgtctggc
     ccccggcctg ttccgtgccc tcaagcgggg cgagcgcaag cgcagcaagc tggacgtgac
     gtatgactac ggcgacggca agcggatcga gttcagcggc ccggagccgc tgggcgctga
     tgatctgcgc atcctgcaag ggctggtggc catggctggg cctaatggcc tagtgcttgg
     cccggaaccc aagaccgaag gcggacggca gctccggctg ttcctggaac ccaagtggga
     ggccgtcacc gctgaatgcc atgtggtcaa aggtagctat cgggcgctgg caaaggaaat
     cggggcagag gtcgatagtg gtggggcgct caagcacata caggactgca tcgagcgcct
     ttggaaggta tccatcatcg cccagaatgg ccgcaagcgg caggggtttc ggctgctgtc
     ggagtacgcc agcgacgagg cggacgggcg cctgtacgtg gccctgaacc ccttgatcgc
     gcaggccgtc atgggtggcg gccagcatgt gcgcatcagc atggacgagg tgcgggcgct
     ggacagcgaa accgcccgcc tgctgcacca gcggctgtgt ggctggatcg accccggcaa
     aaccggcaag gcttccatag ataccttgtg cggctatgtc tggccgtcag aggccagtgg
     ttcgaccatg cgcaagcgcc gccagcgggt gcgcgaggcg ttgccggagc tggtcgcgct
     gggctggacg gtaaccgagt tcgcggcggg caagtacgac atcacccggc ccaaggcggc
     aggctgaccc cccccactct attgtaaaca agacattttt atcttttata ttcaatggct
     tattttcctg ctaattggta ataccatgaa aaataccatg ctcagaaaag gcttaacaat
     attttgaaaa attgcctact gagcgctgcc gcacagctcc ataggccgct ttcctggctt
     tgcttccaga tgtatgctct tctgctcctg caaaagtcag gcaccgtgta tgaaatctaa
     caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt
     tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca
     ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg
     agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac
     tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atctgatcaa gagacaggat
     gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     gctggagttc ttcgcccacc ccgggctcga tcccctcgcg agttggttca gctgctgcct
     gaggctggac gacctcgcgg agttctaccg gcagtgcaaa tccgtcggca tccaggaaac
     cagcagcggc tatccgcgca tccatgcccc cgaactgcaa attcgtt
//