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ID   RSV3       preliminary; circular DNA; SYN; 3557 BP.
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AC   M83240;
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DT   06-JAN-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector RSV.3 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3557
RC   RSV.3 from pRSV-beta-globin & pUC12
RC   RSV.2 from pRSV-beta-globin & pUC12
RA   Jacobson S., Sekaly R.P., Jacobson C.L., McFarland H.F.,
RA   Long E.O.;
RT   "HLA class II-restricted presentation of cytoplasmic measles virus
RT   antigens to cytotoxic T cells";
RL   J. Virol. 63:1756-1762(1989).
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RN   [2]
RC   p220.2 from p201 & linker
RA   Sugden B.;
RT   ;
RL   Unpublished (1989).
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CC   NM (RSV.3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(human)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Rous sarcoma virus)(pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pRSV-beta-globin large HindIII-HpaI,
FT                   \ no beta-globin
FT                   2. pUC12 BamHI-HindIII 27bp 249..276, MCS
FT                   Klenow:
FT                   -> RSV.3 3557bp [no splice]"
FT   misc_binding    1..29
FT                   /note="MCS [3]"
FT   rep_origin      912..3029
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             912..3029
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   enhancer        3030..3557
FT                   /note="PRO Rous sarcoma virus 5' long terminal repeat
FT                   (LTR) [2,3]"
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SQ   Sequence 3557 BP; 930 A; 821 C; 850 G; 956 T; 0 other;
     cttgggctgc aggtcgactc tagaggatca acttgtttat tgcagcttat aatggttaca
     aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccccagg aagctcctct
     gtgtcctcat aaaccctaac ctcctctact tgagaggaca ttccaatcat aggctgccca
     tccaccctct gtgtcctcct gttaattagg tcacttaaca aaaaggaaat tgggtagggg
     tttttcacag accgctttct aagggtaatt ttaaaatatc tgggaagtcc cttccactgc
     tgtgttccag aagtgttggt aaacagccca caaatgtcaa cagcagaaac atacaagctg
     tcagctttgc acaagggccc aacaccctgc tcatcaagaa gcactgtggt tgctgtgtta
     gtaatgtgca aaacaggagg cacattttcc ccacctgtgt aggttccaaa atatctagtg
     ttttcatttt tacttggatc aggaacccag cactccactg gataagcatt atccttatcc
     aaaacagcct tgtggtcagt gttcatctgc tgactgtcaa ctgtagcatt ttttggggtt
     acagtttgag caggatattt ggtcctgtag tttgctaaca caccctgcag ctccaaaggt
     tccccaccaa cagcaaaaaa atgaaaattt gacccttgaa tgggttttcc agcaccattt
     tcatgagttt tttgtgtccc tgaatgcaag tttaacatag cagttacccc aataacctca
     gttttaacag taacagcttc ccacatcaaa atatttccac aggttaagtc ctcatttaaa
     ttaggcaaag gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     gtatctgagg ggactagggt gtgtttaggc gaaaagcggg gcttcggttg tacgcggtta
     ggagtcccct caggatatag tagtttcgct tttgcatagg gagggggaaa tgtagtctta
     tgcaatacac ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     aggaaggcaa cagacaggtc tgacatggat tggacgaacc actgaattcc gcattgcaga
     gataattgta tttaagtgcc tagctcgata caataaacgc catttgacca ttcaccacat
     tggtgtgcac ctccaag
//