Return to this vector's summary.
ID SCOS1 preliminary; circular DNA; SYN; 7937 BP.
XX
AC M99566; IG2060;
XX
DT 04-SEP-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli cosmid vector sCos-1 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC sCos-1 from pWE15 & pDVcos134
RC sCos-2 from sCos-1 & oligo
RC sCos-4 from sCos-1 & oligo
RA Evans G.A., Lewis K., Rothenberg B.E.;
RT "High efficiency vectors for cosmid microcloning and genomic
RT analysis";
RL Gene 79:9-20(1989).
XX
RN [2]
RC from sCos-1
RA Evans G.A., Lewis K.;
RT "Physical mapping of complex genomes by cosmid multiplex analysis";
RL Proc. Natl. Acad. Sci. U.S.A. 86:5030-5034(1989).
XX
RN [3]
RP 1-7937
RC sCos-1 from pWE15 & pDVcos134
RA Evans G.A.;
RT ;
RL Unpublished (1992).
XX
CC NM (sCos-1)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning 31000bp to 48000bp)
CC SE ()
CC PA (lambda)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. lambda, cos
FT -> pDVcos134
FT 1. pWE15 ClaI-SalI 5770bp 8077..8136..5711,
FT \ ori/amp/neo/no cos
FT 2. pDVcos134 ClaI-XhoI 1600bp, cos-XbaI-cos
FT -> sCos-1 7937bp [no XhoI, no SalI]"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cos region"
FT misc_binding 0..0
FT /note="MCS EcoRI-NotI-BamHI-NotI-EcoRI"
FT misc_binding 0..0
FT /note="SIT EcoRII"
FT misc_binding 0..0
FT /note="SIT NotI"
FT promoter 0..0
FT /note="PRO bacteriophage T7"
FT misc_binding 0..0
FT /note="SIT BamHI"
FT promoter complement(0..0)
FT /note="PRO bacteriophage T3"
FT misc_binding 0..0
FT /note="SIT NotI"
FT misc_binding 0..0
FT /note="SIT EcoRI"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="ANT E. coli neomycin phosphotransferase II gene
FT (NPTII); neomycin resistance gene (neo)"
FT CDS 0..0
FT /note="ANT E. coli kanamycin resistance gene (kan)"
FT CDS 0..0
FT /note="ANT mammal G418 resistance gene (G418)"
FT rep_origin 0..0
FT /note="ORI SV40"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda"
FT misc_binding 0..0
FT /note="SIT XbaI"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cos region"
XX
SQ Sequence 7937 BP; 1949 A; 2033 C; 1988 G; 1967 T; 0 other;
gaattcgcgg ccgcaattaa ccctcactaa aggatcccta tagtgagtcg tattatgcgg
ccgcgaattc tcatgtttga ccgcttatca tcgataagct ctgctttttg ttgacttcca
ttgttcattc cacggacaaa aacagagaaa ggaaacgaca gaggccaaaa agctcgcttt
cagcacctgt cgtttccttt cttttcagag ggtattttaa ataaaaacat taagttatga
cgaagaagaa cggaaacgcc ttaaaccgga aaattttcat aaatagcgaa aacccgcgag
gtcgccgccc cgtaacaagg cggatcgccg gaaaggaccc gcaaatgata ataattatca
attgcatact atcgacggca ctgctgccag ataacaccac cggggaaaca ttccatcatg
atggccgtgc ggacatagga agccagttca tccatcgctt tcttgtctgc tgccatttgc
tttgtgacat ccagcgccgc acattcagca gcgtttttca gcgcgttttc gatcaacgtt
tcaatgttgg tatcaacacc aggtttaact ttgaacttat cggcactgac ggttaccttg
ttctgcgctg gctcatcacg caggatacca aggctgatgt tgtagatatt ggtcaccggc
tgagggtttt cgattgccgc tgcgtggata gcaccatttg cgatcaggcg tccttgatga
atgacactcc attgcgaata agttcgaagg agacggtgtc acgaatgcgc tggtccagct
cggtcgattg ccttttgtgc agcagaggta tcaatctcaa cgccaaggct catcgaagcg
caatattgct gctcaccaaa acgcgtattg accaggtgtt caacggcaaa tttctgccct
tctgatgtca gaaaggcaaa gtgattttct ttctggtatt cagttgctgt gtgtcggttt
cagcaaaacc aagctcgcgc aattcggctg tgcagattta gaaggcagat caccagacag
caacggccaa cggaaaacag cgcatacaga acatccgtcg ccgcgccgac aacgtgataa
tttttatgac ccatgattta tttcctttta gacgtgagcc tgtcgcacag caaagccgcc
gaaagttcct cgaagctagc ttcagacgtg tctagatacg tctgcttttt gttgacttcc
attgttcatt ccacggacaa aaacagagaa aggaaacgac agaggccaaa aagctcgctt
tcagcacctg tcgtttcctt tcttttcaga gggtatttta aataaaaaca ttaagttatg
acgaagaaga acggaaacgc cttaaaccgg aaaattttca taaatagcga aaacccgcga
ggtcgccgcc ccgtaacaag gcggatcgcc ggaaaggacc cgcaaatgat aataattatc
aattgcatac tatcgacggc actgctgcca gataacacca ccggggaaac attccatcat
gatggccgtg cggacatagg aagccagttc atccatcgct ttcttgtctg ctgccatttg
ctttgtgaca tccagcgccg cacattcagc agcgtttttc agcgcgtttt cgatcaacgt
ttcaatgttg gtatcaacac caggtttaac tttgaactta tcggcactga cggttacctt
gttctgcgct ggctcatcac gcaggatacc aaggctgatg ttgtagatat tggtcaccgg
ctgagggttt tcgattgccg ctgcgtggat agcaccattt gcgatcaggc gtccttgatg
aatgacactc cattgcgaat aagttcgaag gagacggtgt cacgaatgcg ctggtccagc
tcggtcgatt gccttttgtg cagcagaggt atcaatctca acgccaaggc tcatcgaagc
gcaatattgc tgctcaccaa aacgcgtatt gaccaggtgt tcaacggcaa atttctgccc
ttctgatgtc agaaaggcaa agtgattttc tttctggtat tcagttgctg tgtgtcggtt
tcagcaaaac caagctcgcg caattcggct gtgcagattt agaaggcaga tcaccagaca
gcaacggcca acggaaaaca gcgcatacag aacatccgtc gccgcgccga caacgtgata
atttttatga cccatgattt atttcctttt agacgtgagc ctgtcgcaca gcaaagccgc
cgaaagttcc tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa
cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca
aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg
catctcgggc agcgttgggt cctgcagatc cggctgtgga atgtgtgtca gttagggtgt
ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
ggcttttgca aaaagcttca cgctgccgca agcactcagg gcgcaagggc tgctaaagga
agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat
cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
gttcttcgcc caccccgggc tcgatcccct cgcgagttgg ttcagctgct gcctgaggct
ggacgacctc gcggagttct accggcagtg caaatccgtc ggcatccagg aaaccagcag
cggctatccg cgcatccatg cccccgaact gcaggagtgg ggaggcacga tggccgcttt
ggtccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
cttatcatgt ctggatctga cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt
tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgct cttccgcttc ctcgctcact
gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
ggccctttcg tcttcaa
//