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ID   SCOS1      preliminary; circular DNA; SYN; 7937 BP.
XX
AC   M99566; IG2060;
XX
DT   04-SEP-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli cosmid vector sCos-1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   sCos-1 from pWE15 & pDVcos134
RC   sCos-2 from sCos-1 & oligo
RC   sCos-4 from sCos-1 & oligo
RA   Evans G.A., Lewis K., Rothenberg B.E.;
RT   "High efficiency vectors for cosmid microcloning and genomic
RT   analysis";
RL   Gene 79:9-20(1989).
XX
RN   [2]
RC   from sCos-1
RA   Evans G.A., Lewis K.;
RT   "Physical mapping of complex genomes by cosmid multiplex analysis";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:5030-5034(1989).
XX
RN   [3]
RP   1-7937
RC   sCos-1 from pWE15 & pDVcos134
RA   Evans G.A.;
RT   ;
RL   Unpublished (1992).
XX
CC   NM (sCos-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning 31000bp to 48000bp)
CC   SE ()
CC   PA (lambda)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda, cos
FT                   -> pDVcos134
FT                   1. pWE15 ClaI-SalI 5770bp 8077..8136..5711,
FT                   \ ori/amp/neo/no cos
FT                   2. pDVcos134 ClaI-XhoI 1600bp, cos-XbaI-cos
FT                   -> sCos-1 7937bp [no XhoI, no SalI]"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cos region"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-NotI-BamHI-NotI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRII"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII); neomycin resistance gene (neo)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT mammal G418 resistance gene (G418)"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cos region"
XX
SQ   Sequence 7937 BP; 1949 A; 2033 C; 1988 G; 1967 T; 0 other;
     gaattcgcgg ccgcaattaa ccctcactaa aggatcccta tagtgagtcg tattatgcgg
     ccgcgaattc tcatgtttga ccgcttatca tcgataagct ctgctttttg ttgacttcca
     ttgttcattc cacggacaaa aacagagaaa ggaaacgaca gaggccaaaa agctcgcttt
     cagcacctgt cgtttccttt cttttcagag ggtattttaa ataaaaacat taagttatga
     cgaagaagaa cggaaacgcc ttaaaccgga aaattttcat aaatagcgaa aacccgcgag
     gtcgccgccc cgtaacaagg cggatcgccg gaaaggaccc gcaaatgata ataattatca
     attgcatact atcgacggca ctgctgccag ataacaccac cggggaaaca ttccatcatg
     atggccgtgc ggacatagga agccagttca tccatcgctt tcttgtctgc tgccatttgc
     tttgtgacat ccagcgccgc acattcagca gcgtttttca gcgcgttttc gatcaacgtt
     tcaatgttgg tatcaacacc aggtttaact ttgaacttat cggcactgac ggttaccttg
     ttctgcgctg gctcatcacg caggatacca aggctgatgt tgtagatatt ggtcaccggc
     tgagggtttt cgattgccgc tgcgtggata gcaccatttg cgatcaggcg tccttgatga
     atgacactcc attgcgaata agttcgaagg agacggtgtc acgaatgcgc tggtccagct
     cggtcgattg ccttttgtgc agcagaggta tcaatctcaa cgccaaggct catcgaagcg
     caatattgct gctcaccaaa acgcgtattg accaggtgtt caacggcaaa tttctgccct
     tctgatgtca gaaaggcaaa gtgattttct ttctggtatt cagttgctgt gtgtcggttt
     cagcaaaacc aagctcgcgc aattcggctg tgcagattta gaaggcagat caccagacag
     caacggccaa cggaaaacag cgcatacaga acatccgtcg ccgcgccgac aacgtgataa
     tttttatgac ccatgattta tttcctttta gacgtgagcc tgtcgcacag caaagccgcc
     gaaagttcct cgaagctagc ttcagacgtg tctagatacg tctgcttttt gttgacttcc
     attgttcatt ccacggacaa aaacagagaa aggaaacgac agaggccaaa aagctcgctt
     tcagcacctg tcgtttcctt tcttttcaga gggtatttta aataaaaaca ttaagttatg
     acgaagaaga acggaaacgc cttaaaccgg aaaattttca taaatagcga aaacccgcga
     ggtcgccgcc ccgtaacaag gcggatcgcc ggaaaggacc cgcaaatgat aataattatc
     aattgcatac tatcgacggc actgctgcca gataacacca ccggggaaac attccatcat
     gatggccgtg cggacatagg aagccagttc atccatcgct ttcttgtctg ctgccatttg
     ctttgtgaca tccagcgccg cacattcagc agcgtttttc agcgcgtttt cgatcaacgt
     ttcaatgttg gtatcaacac caggtttaac tttgaactta tcggcactga cggttacctt
     gttctgcgct ggctcatcac gcaggatacc aaggctgatg ttgtagatat tggtcaccgg
     ctgagggttt tcgattgccg ctgcgtggat agcaccattt gcgatcaggc gtccttgatg
     aatgacactc cattgcgaat aagttcgaag gagacggtgt cacgaatgcg ctggtccagc
     tcggtcgatt gccttttgtg cagcagaggt atcaatctca acgccaaggc tcatcgaagc
     gcaatattgc tgctcaccaa aacgcgtatt gaccaggtgt tcaacggcaa atttctgccc
     ttctgatgtc agaaaggcaa agtgattttc tttctggtat tcagttgctg tgtgtcggtt
     tcagcaaaac caagctcgcg caattcggct gtgcagattt agaaggcaga tcaccagaca
     gcaacggcca acggaaaaca gcgcatacag aacatccgtc gccgcgccga caacgtgata
     atttttatga cccatgattt atttcctttt agacgtgagc ctgtcgcaca gcaaagccgc
     cgaaagttcc tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa
     cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca
     aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg
     catctcgggc agcgttgggt cctgcagatc cggctgtgga atgtgtgtca gttagggtgt
     ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     ggcttttgca aaaagcttca cgctgccgca agcactcagg gcgcaagggc tgctaaagga
     agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
     actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
     ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
     cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
     tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat
     cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     gttcttcgcc caccccgggc tcgatcccct cgcgagttgg ttcagctgct gcctgaggct
     ggacgacctc gcggagttct accggcagtg caaatccgtc ggcatccagg aaaccagcag
     cggctatccg cgcatccatg cccccgaact gcaggagtgg ggaggcacga tggccgcttt
     ggtccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
     catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
     aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
     tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     cttatcatgt ctggatctga cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
     taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
     gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt
     tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgct cttccgcttc ctcgctcact
     gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     ggccctttcg tcttcaa
//