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ID   SV40       standard; circular DNA; VRL; 5243 BP.
XX
AC   V01380;
XX
DT   01-JUL-1995 (Rel. 01, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Monkey virus SV40 - complete.
XX
KW   cloning vector.
XX
OS   Rhesus macaque polyomavirus
OC   Viridae; ds-DNA nonenveloped viruses; Papovaviridae;
OC   Polyomaviruses.
XX
RN   [1]
RP   1-5243
RC   SV40 from monkey
RA   Fiers W., Contreras R.R., Haegeman G., Rogiers R., De Voorde A.,
RA   van Heuverswyn H., Van Herreweghe J., Volckaert G., Ysebaert M.;
RT   "Complete nucleotide sequence of SV40 DNA";
RL   Nature 273:113-120(1978).
XX
RN   [2]
RP   1-5243
RC   SV40 from monkey
RA   Buchman A.R., Burnett L., Berg P.;
RT   "The SV40 nucleotide sequence";
RL   DNA Tumor Viruses [2nd edition] 0:799-823(1980).
RL   Tooze J., ed., Cold Spring Harbor Laboratory.
XX
RN   [3]
RC   SV40 from monkey
RA   Everett R.D., Baty D., Chambon P.;
RT   "The repeated GC-rich motifs upstream from the TATA box are
RT   important elements of the SV40 early promoter";
RL   Nucleic Acids Res. 11:2447-2464(1983).
XX
RN   [4]
RC   SV40 from monkey
RA   Noble J.C., Prives C., Manley J.L.;
RT   "In vitro splicing of simian virus 40 early pre mRNA";
RL   Nucleic Acids Res. 14:1219-1235(1986).
XX
RN   [5]
RP   5165-5243
RC   SV40 from monkey
RA   Tenen D.G., Taylor T.S., Haines L.L., Bradley M.K., Martin R.G.,
RA   Livingston D.M.;
RT   "Binding of simian virus 40 large T antigen from virus-infected
RT   monkey cells to wild-type and mutant viral replication origins";
RL   J. Mol. Biol. 168:791-808(1983).
XX
RN   [6]
RC   SV40 from monkey
RA   Barrera-Saldana H., Takahashi K., Vigneron M., Wildeman A.,
RA   Davidson I., Chambon P.;
RT   "All six GC-motifs of the SV40 early upstream element contribute
RT   to promoter activity in vivo and in vitro";
RL   EMBO J. 4:3839-3849(1985).
XX
CC   Sequence was taken from reference [2].
CC   NM (SV40)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (virus)
CC   SP ()
CC   HO (monkey)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (monkey)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. monkey
FT                   -> SV40 5243bp"
FT   promoter        complement(15..21)
FT                   /note="TATA-box [3]"
FT   repeat_region   40..61
FT                   /note="imperfect 32 bp repeat (early promoter)"
FT   repeat_region   62..82
FT                   /note="21 bp repeat (early promoter)"
FT   repeat_region   83..103
FT                   /note="21 bp repeat (early promoter)"
FT   repeat_region   107..178
FT                   /note="72 bp repeat (early promoter)"
FT   mRNA            120..2674
FT                   /note="major 16S RNA"
FT   repeat_region   179..250
FT                   /note="72 bp repeat (early promoter)"
FT   CDS             562..1620
FT                   /product="VP2"
FT   CDS             916..1620
FT                   /product="VP3"
FT   CDS             1499..2593
FT                   /product="VP1"
FT   CDS             join(complement(4918..5163),complement(2691..4571))
FT                   /product="large T"
FT   mRNA            complement(2587..4571)
FT                   /note="mRNA of small t (part 2)"
FT   mRNA            complement(2587..4571)
FT                   /note="mRNA of large T (part 2)"
FT   CDS             complement(4639..5163)
FT                   /product="small t"
FT   mRNA            complement(4918..5235)
FT                   /note="mRNA of large T (part 1)"
FT   mRNA            complement(4638..5235)
FT                   /note="mRNA of small t (part 1)"
XX
SQ   Sequence 5243 BP; 1518 A; 1100 C; 1039 G; 1586 T; 0 other;
     gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
     ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
     ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
     gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca gaaggtacct
     aaccaagttc ctctttcaga ggttatttca ggccatggtg ctgcgccggc tgtcacgcca
     ggcctccgtt aaggttcgta ggtcatggac tgaaagtaaa aaaacagctc aacgcctttt
     tgtgtttgtt ttagagcttt tgctgcaatt ttgtgaaggg gaagatactg ttgacgggaa
     acgcaaaaaa ccagaaaggt taactgaaaa accagaaagt taactggtaa gtttagtctt
     tttgtctttt atttcaggtc catgggtgct gctttaacac tgttggggga cctaattgct
     actgtgtctg aagctgctgc tgctactgga ttttcagtag ctgaaattgc tgctggagag
     gccgctgctg caattgaagt gcaacttgca tctgttgcta ctgttgaagg cctaacaacc
     tctgaggcaa ttgctgctat aggcctcact ccacaggcct atgctgtgat atctggggct
     cctgctgcta tagctggatt tgcagcttta ctgcaaactg tgactggtgt gagcgctgtt
     gctcaagtgg ggtatagatt ttttagtgac tgggatcaca aagtttctac tgttggttta
     tatcaacaac caggaatggc tgtagatttg tataggccag atgattacta tgatatttta
     tttcctggag tacaaacctt tgttcacagt gttcagtatc ttgaccccag acattggggt
     ccaacacttt ttaatgccat ttctcaagct ttttggcgtg taatacaaaa tgacattcct
     aggctcacct cacaggagct tgaaagaaga acccaaagat atttaaggga cagtttggca
     aggtttttag aggaaactac ttggacagta attaatgctc ctgttaattg gtataactct
     ttacaagatt actactctac tttgtctccc attaggccta caatggtgag acaagtagcc
     aacagggaag ggttgcaaat atcatttggg cacacctatg ataatattga tgaagcagac
     agtattcagc aagtaactga gaggtgggaa gctcaaagcc aaagtcctaa tgtgcagtca
     ggtgaattta ttgaaaaatt tgaggctcct ggtggtgcaa atcaaagaac tgctcctcag
     tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaagcttat
     gaagatggcc ccaacaaaaa gaaaaggaag ttgtccaggg gcagctccca aaaaaccaaa
     ggaaccagtg caagtgccaa agctcgtcat aaaaggagga atagaagttc taggagttaa
     aactggagta gacagcttca ctgaggtgga gtgcttttta aatcctcaaa tgggcaatcc
     tgatgaacat caaaaaggct taagtaaaag cttagcagct gaaaaacagt ttacagatga
     ctctccagac aaagaacaac tgccttgcta cagtgtggct agaattcctt tgcctaattt
     aaatgaggac ttaacctgtg gaaatatttt gatgtgggaa gctgttactg ttaaaactga
     ggttattggg gtaactgcta tgttaaactt gcattcaggg acacaaaaaa ctcatgaaaa
     tggtgctgga aaacccattc aagggtcaaa ttttcatttt tttgctgttg gtggggaacc
     tttggagctg cagggtgtgt tagcaaacta caggaccaaa tatcctgctc aaactgtaac
     cccaaaaaat gctacagttg acagtcagca gatgaacact gaccacaagg ctgttttgga
     taaggataat gcttatccag tggagtgctg ggttcctgat ccaagtaaaa atgaaaacac
     tagatatttt ggaacctaca caggtgggga aaatgtgcct cctgttttgc acattactaa
     cacagcaacc acagtgcttc ttgatgagca gggtgttggg cccttgtgca aagctgacag
     cttgtatgtt tctgctgttg acatttgtgg gctgtttacc aacacttctg gaacacagca
     gtggaaggga cttcccagat attttaaaat tacccttaga aagcggtctg tgaaaaaccc
     ctacccaatt tcctttttgt taagtgacct aattaacagg aggacacaga gggtggatgg
     gcagcctatg attggaatgt cctctcaagt agaggaggtt agggtttatg aggacacaga
     ggagcttcct ggggatccag acatgataag atacattgat gagtttggac aaaccacaac
     tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     ggctgattat gatcatgaac agactgtgag gactgagggg cctgaaatga gccttgggac
     tgtgaatcaa tgcctgtttc atgccctgag tcttccatgt tcttctcccc accatcttca
     tttttatcag cattttcctg gctgtcttca tcatcatcat cactgtttct tagccaatct
     aaaactccaa ttcccatagc cacattaaac ttcatttttt gatacactga caaactaaac
     tctttgtcca atctctcttt ccactccaca attctgctct gaatactttg agcaaactca
     gccacaggtc tgtaccaaat taacataaga agcaaagcaa tgccactttg aattattctc
     ttttctaaca aaaactcact gcgttccagg caatgcttta aataatcttt gggcctaaaa
     tctatttgtt ttacaaatct ggcctgcagt gttttaggca cactgtactc attcatggtg
     actattccag ggggaaatat ttgagttctt ttatttaggt gtttcttttc taagtttacc
     ttaacactgc catccaaata atcccttaaa ttgtccaggt tattaattcc ctgacctgaa
     ggcaaatctc tggactcccc tccagtgccc tttacatcct caaaaactac taaaaactgg
     tcaatagcta ctcctagctc aaagttcagc ctgtccaagg gcaaattaac atttaaagct
     ttccccccac ataattcaag caaagcagct gctaatgtag ttttaccact atcaattggt
     cctttaaaca gccagtatct ttttttagga atgttgtaca ccatgcattt taaaaagtca
     tacaccactg aatccatttt gggcaacaaa cagtgtagcc aagcaactcc agccatccat
     tcttctatgt cagcagagcc tgtagaacca aacattatat ccatcctatc caaaagatca
     ttaaatctgt ttgttaacat ttgttctcta gttaattgta ggctatcaac ccgcttttta
     gctaaaacag tatcaacagc ctgttggcat atggtttttt ggtttttgct gtcagcaaat
     atagcagcat ttgcataatg cttttcatgg tacttatagt ggctgggctg ttctttttta
     atacatttta aacacatttc aaaactgtac tgaaattcca agtacatccc aagcaataac
     aacacatcat cacattttgt ttccattgca tactctgtta caagcttcca ggacacttgt
     ttagtttcct ctgcttcttc tggattaaaa tcatgctcct ttaacccacc tggcaaactt
     tcctcaataa cagaaaatgg atctctagtc aaggcactat acatcaaata ttccttatta
     acccctttac aaattaaaaa gctaaaggta cacaattttt gagcatagtt attaatagca
     gacactctat gcctgtgtgg agtaagaaaa aacagtatgt tatgattata actgttatgc
     ctacttataa aggttacaga atatttttcc ataattttct tgtatagcag tgcagctttt
     tcctttgtgg tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc
     aaaaaactta gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt
     ggaggagtag aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct
     gagcaaaaca ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca
     taggttggaa tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt
     aaaaatttta tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc
     acaccacaga agtaaggttc cttcacaaag atcaagtcca aaccacattc taaagcaatc
     gaagcagtag caatcaaccc acacaagtgg atctttcctg tataattttc tattttcatg
     cttcatcctc agtaagcaca gcaagcatat gcagttagca gacattttct ttgcacactc
     aggccattgt ttgcagtaca ttgcatcaac accaggattt aaggaagaag caaatacctc
     agttgcatcc cagaagcctc caaagtcagg ttgatgagca tattttactc catcttccat
     tttcttgtac agagtattca ttttcttcat tttttcttca tctcctcctt tatcaggatg
     aaactccttg cattttttta aatatgcctt tctcatcaga ggaatattcc cccaggcact
     cctttcaaga cctagaaggt ccattagctg caaagattcc tctctgttta aaactttatc
     catctttgca aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     ggaatagctc agaggccgag gcg
//