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ID   SVPHEP     preliminary; circular DNA; SYN; 9201 BP.
XX
AC   L19900;
XX
DT   24-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli cosmid vector svPHEP - complete.
XX
KW   cloning vector; beta-lactamase; thymidine kinase.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-9201
RC   svPHEP from pBR322 & HSV tk gene
RA   Bodenteich A., Chissoe S.L., Wang Z., Roe B.A.;
RT   "Nucleotide sequence of three cosmid cloning vectors: svPHEP, pTCF,
RT   and LAWRIST16";
RL   Unpublished (1993).
XX
CC   Constructed by J. Groffen and F. Grosveld,
CC   Department of Cell Biology, Erasmus Univ., Rotterdam, Netherlands.
CC   NCBI gi: 310846
CC   NM (svPHEP)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. HSV, tk gene/#X14112
FT                   -> svPHEP 9201bp"
FT   CDS             complement(3344..4133)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp);
FT                   10 stops when translation attempted, frame 1, code 0"
FT   CDS             837..1967
FT                   /note="GEN HSV thymidine kinase gene (tk);
FT                   NCBI gi: 310847"
XX
SQ   Sequence 9201 BP; 2063 A; 2489 C; 2603 G; 2046 T; 0 other;
     ggatccaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
     gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
     cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
     cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
     gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
     gcggcaacgg gctcgctggg tcctaggctc aatggggacc gtatacgtgg acaggctctg
     gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac
     gcggctgacc ggagcgtccg ttgggcgaca aacaccagga cggggcacag gtacactatc
     ttgtcacccg gaggcgcgag ggactgcagg agcttcaggg agtggcgcag ctgcttcatc
     cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg aagaaatata tttgcatgtc
     tttagttcta tgatgacaca aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg
     cagatgcagt cggggcggcg cggtcccagg tccacttcgc atattaaggt gacgcgtgtg
     gcctcgaata ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag
     atcttggtgg cgtgaaactc ccgcacctct tcggccagcg ccttgtagaa gcgcgtatgg
     cttcgtaccc ctgccatcaa cacgcgtctg cgttcgacca ggctgcgcgt tctcgcggcc
     ataacaaccg acgtacggcg ttgcgccctc gccggcagca aaaagccacg gaagtccgcc
     tggagcagaa aatgcccacg ctactgcggg tttatataga cggtccccac gggatgggga
     aaaccaccac cacgcaactg ctggtggccc tgggttcgcg cgacgatatc gtctacgtac
     ccgagccgat gacttactgg cgggtgttgg gggcttccga gacaatcgcg aacatctaca
     ccacacaaca ccgcctcgac cagggtgaga tatcggccgg ggacgcggcg gtggtaatga
     caagcgccca gataacaatg ggcatgcctt atgccgtgac cgacgccgtt ctggctcctc
     atatcggggg ggaggctggg agctcacatg ccccgccccc ggccctcacc ctcatcttcg
     accgccatcc catcgccgcc ctcctgtgct acccggccgc gcgatacctt atgggcagca
     tgacccccca ggccgtgctg gcgttcgtgg ccctcatccc gccgaccttg cccggcacaa
     acatcgtgtt gggggccctt ccggaggaca gacacatcga ccgcctggcc aaacgccagc
     gccccggcga gcggcttgac ctggctatgc tggccgcgat tcgccgcgtt tatgggctgc
     ttgccaatac ggtgcggtat ctgcagggcg gcgggtcgtg gcgggaggat tggggacagc
     tttcgggggc ggccgtgccg ccccagggtg ccgagcccca gagcaacgcg ggcccacgac
     cccatatcgg ggacacgtta tttaccctgt ttcgggcccc cgagttgctg gcccccaacg
     gcgacctgta taacgtgttt gcctgggctt tggacgtctt ggccaaacgc ctccgtccca
     tgcacgtctt tatcctggat tacgaccaat cgcccgccgg ctgccgggac gccctgctgc
     aacttacctc cgggatggtc cagacccacg tcaccacccc aggctccata ccgacgatct
     gcgacctggc gcgcacgttt gcccgggaga tgggggaggc taactgaaac acggaaggag
     acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg
     tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     cccaccgaga ccccattggg accaatacgc ccgcgtttct tccttttccc caccccaacc
     cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcaa gccctgccat
     agccacgggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg
     ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac
     ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg
     cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgccg
     ccggacgaac taaacctgac tacggcatct ctgccccttc ttcgctggta cgaggagcgc
     ttttgttttg tattggtcac cacggccgag tttccgcggg accccggcca gctgctttac
     atcccgaaga cctacctgct cggccggccc ccgaacgcga gcctgcccgc ccccaccacg
     gtcgagccga ccgcccagcc tcccccctcg gtcgcccccc ttaagggtct cttgcacaat
     ccagccgcct ccgtgttgct gcgttcccgg gcctgggtaa cgttttcggc cgtccctgac
     cccgaggccc tgacgttccc gcggggagac aacgtggcga cggcgagcca cccgagcggg
     ccgcgtgata caccgccccc ccgaccgccg gttggggccc ggcggcaccc gacgacggag
     ctggacatca cgcacctgca caacgcgtcc acgacctggt tggccacccg gggcctgttg
     agatccccag gtaggtacgt gtatttctcc ccgtcggcct cgacgtggcc cgtgggcatc
     tggacgacgg gggagctggt gctcgggtgc gatgccgcgc tggtgcgcgc gcgctacggg
     cgggaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cccgagatgc
     gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt
     gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt
     tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg
     caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca
     tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga tcgaagttag
     gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct
     ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
     ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc
     cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac
     gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
     cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
     tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
     ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt
     atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc
     cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc
     caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc
     atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
     cacgatgcgt ccggcgtaga ggatcttggc agtcacagca tgcgcatatc catgcttcga
     ccatgcgctc acaaagtagg tgaatgcgca atgtagtacc cacatcgtca tcgctttcca
     ctgctctcgc gaataaagat ggaaaatcaa tctcatggta atagtccatg aaaatccttg
     tattcataaa tcctccaggt agctatatgc aaattgaaac aaaagagatg gtgatctttc
     taagagatga tggaatctcc cttcagtatc ccgatggtca atgcgctgga tatgggatag
     atgggaatat gctgattttt atgggacaga gttgcgaact gttcccaact aaaatcattt
     tgcacgatca gcgcactacg aactttaccc acaaatagtc aggtaatgaa tcctgatata
     aagacaggtt gataaatcag tcttctacgc gcatcgcacg cgcacaccgt agaaagtctt
     tcagttgtga gcctgggcaa accgttaact ttcggcggct ttgctgtgcg acaggctcac
     gtctaaaagg aaataaatca tgggtcataa aattatcacg ttgtccggcg cggcgacgga
     tgttctgtat gcgctgtttt tccgtggcgc gttgctgtct ggtgatctgc cttctaaatc
     tggcacagcc gaattgcgcg agcttggttt tgctgaaacc agacacacag caactgaata
     ccagaaagaa aatcacttta cctttctgac atcagaaggg cagaaatttg ccgttgaaca
     cctggtcaat acgcgttttg gtgagcagca atattgcgct tcgatgacgc ttggcgttga
     gattgatacc tctgctgcac aaaaggcaat cgacgagctg gaccagcgca ttcgtgacac
     cgtctccttc gaacttattc gcaatggagt gtcattcatc aaggacgccg ctatcgcaaa
     tggtgctatc cacgcagcgg caatcgaaac acctcagccg gtgaccaata tctacaacat
     cagccttggt atccagcgtg atgagccagc gcagaacaag gtaaccgtca gtgccgataa
     gttcaaagtt aaacctggtg ttgataccaa cattgaaacg ttgatcgaaa acgcgctgaa
     aaacgctgct gaatgtgcgg cgctggatgt cacaaagcaa atggcagcag acaagaaagc
     gatggatgaa ctggcttcct atgtccgcac ggccatcatg atggaatgtt tccccggtgg
     tgttatctgg cagcagtgcc gtcgatagta tgcaattgat aattattatc atttgcgggt
     cctttccggc gatccgcctt gttacggggc ggcgacctcg cgggttttcg ctatttatga
     aaattttccg gtttaaggcg tttccgttct tcttcgtcat aacttaatgt ttttatttaa
     aataccctct gaaaagaaag gaaacgacag gtgctgaaag cgagcttttt ggcctctgtc
     gtttcctttc tctgtttttg tccgtggaat gaacaatgga agtcaacaaa aagcagctgg
     ctgacatttt cggtgcgagt atccgtacca ttcagaactg gcaggaacag ggaatgcccg
     ttctgcgagg cggtggcaag ggtaatgagg tgctttatga ctctgccgcc gtcataaaat
     ggtatgccga aagggatgct gaaattgaga acgaaaagct gcgccgggag gttgaaggac
     tgcggcaggc cagcgaggca gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg
     atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt
     gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca
     tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc
     ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca
     ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg
     cattaaagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
     agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
     ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
     actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     gctggggagc ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt
     ccgcctcaga aggtacctaa ccaagttcct ctttcagagg ttatttcagg ccatggtgct
     gcgccggctg tcacgccagg cctccgttaa ggttcgtagg tcatggactg aaagtaaaaa
     aacagctcaa cgcctttttg tgtttgtttt agagcttttg ctgcaatttt gtgaagggga
     agatactgtt gacgggaaac gcaaaaaacc agaaaggtta actgaaaaac cagaaagtta
     actggtaagt ttagtctttt tgtcttttat ttcaggtcca tgggtgctgc tttaacactg
     ttgggggacc taattgctac tgtgtctgaa gctgctgctg ctactggatt ttcagtagct
     gaaattgctg ctggagaggc cgctgctgca attgaagtgc aacttgcatc tgttgctact
     gttgaaggcc taacaacctc tgaggcaatt gctgctatag gcctcactcc acaggcctat
     gctgtgatat ctggggctcc tgctgctata gctggatttg cagctttact gcaaactgtg
     actggtgtga gcgctgttgc tcaagtgggg tatagatttt ttagtgactg ggatcacaaa
     gtttctactg ttggtttata tcaacaacca ggaatggctg tagatttgta taggccagat
     gattattatg atattttatt tcctggagta caaacctttg ttcacagtgt tcagtatctt
     gaccccagac attggggtcc aacacttttt aatgccattt ctcaagctta tcgatgataa
     gctgtcaaac atgagaattc c
//