Return to this vector's summary.
ID YCP50 preliminary; circular DNA; SYN; 7950 BP.
XX
AC X70276; K03077; ATCC37419;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Saccharomyces/E.coli plasmid vector YCp50 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC from YCp50 & yeast genes
RC pRSa4-8 from YEp13 & yeast genes
RC pCT80 from pUC12 & pRSa4-8, topoisomerase I gene
RA Thrash C., Bankier A.T., Barrell B.G., Sternglanz R.;
RT "Cloning, characterization, and sequence of the yeast DNA
RT topoisomerase I gene";
RL Proc. Natl. Acad. Sci. U.S.A. 82:4374-4378(1985).
XX
RN [2]
RC from YCp50
RA Rose M., Novick P., Thomas J.H., Botstein D., Fink G.R.;
RT "A Saccharomyces cerevisiae genomic plasmid bank based on a
RT centromere-containing shuttle vector";
RL Gene 60:237-243(1987).
XX
RN [3]
RP 1-7950
RC YCp50
RA Stillman D.J.;
RT ;
RL Submitted (19-MAY-1993) by:
RL Stillman D.J., University of Utah, Utah.
XX
RN [4]
RC GAL Sc4911 from GAL gene
RC GAL Sc4812 from GAL gene
RC pBM45 from YRp14 & GAL Sc4911 & GAL Sc4812
RC galI-111, galI-112, galI-152, galI-227 from pBM45
RC YCp50 from YCp19 & YIp5
RC pBM125 from YCp50 & galI-152
RC pBM150 from pBM125
RA Johnston M., Davis R.W.;
RT "Sequences that regulate the divergent GAL1-GAL10 promoter in
RT Saccharomyces cerevisiae";
RL Mol. Cell. Biol. 4:1440-1448(1984).
XX
CC YCp50 was constructed by removing the EcoRI site in YCp19 between ARS1
CC and CEN4 and cloning the PvuII/HindIII fragment into the PvuII site of
CC YIp5.
CC The PvuII site was not regenerated. [2]
CC YC-type shuttle vector.
CC The order of the major features in this plasmid is: HindIII - BamHI -
CC SphI - URA3 - CEN4 - ARS1 - pMB1 ori - bla.
CC Medium is 1227 LB plus ampicillin.
CC
CC To pBR322, insert 1.1 kb HindIII-HindIII URA3 fragment into
CC AvaI site with no overhangs and 10 G's added to nt 1428-1437
CC and 10 C's added to nt 2601-2610 [dG/dC tailing] to make YIp5.
CC
CC Ligate CEN4 from PvuII to EcoRI to ARS1 from EcoR1 to Hind III.
CC Delete 190 nt around EcoRI site [85 nt from each side]. BalI site is
CC deleted; perhaps it is intact in [2].
CC
CC Insert CEN4-ARS1 fragment [PvuII - CEN4 - dead EcoRI - ARS1 -
CC HindIII/klenowed] into PvuII site of YIp5. Change CAGCTG to CAGGTG
CC so PvuII site is not regenerated and restriction site numbering
CC of [2] is retained.
CC NCBI gi: 406851
CC NM (YCp50)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC CP ()
CC FN (cloning)(shuttle)
CC SE ()
CC PA (pBR322)(YIp5)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. YCp19 EcoRI 10600bp, between ARS1 and CEN4
FT S1 nuclease
FT fill in
FT PvuII-HindIII 2400bp, ARS1/CEN4/YRp17
FT \ YRp17 1494..-..4711
FT :Klenow
FT 2. YIp5 PvuII 5541bp 3250..3250
FT -> YCp50 7950bp [no PvuII]"
FT misc_feature join(1..1429,2611..3247,5657..7950)
FT /note="from pBR322"
FT misc_feature join(1430..2600,3248..5656)
FT /note="from Saccharomyces cerevisiae"
FT misc_feature 1..1429
FT /note="from pBR322; EcoRI to AvaII, blunted"
FT misc_feature 1429..1438
FT /note="poly dG-dC tail"
FT CDS 1439..2600
FT /note="ANT yeast URA3 gene"
FT misc_feature 2601..2610
FT /note="poly dG-dC tail"
FT misc_feature 2611..3247
FT /note="from pBR322; AvaII, blunted to PvuII;
FT PvuII site not regenerated"
FT misc_feature 3248..4911
FT /note="yeast centromere CEN4"
FT rep_origin 4912..5656
FT /note="ORI yeast ARS1"
FT misc_feature 5657..7950
FT /note="from pBR322; PvuII to EcoRI"
FT misc_binding 0..0
FT /note="SIT unique EcoRI-BglII-KpnI-XhoI-HpaI-SmaI-
FT ClaI-BstXI-NsiI(AvaIII)-
FT BamHI-HindIII-ApaI-NcoI-SalI-PvuI-NruI-SphI-XmaIII-
FT AsuII-BspmII-SpeI-AatII"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ Sequence 7950 BP; 2142 A; 1852 C; 1811 G; 2145 T; 0 other;
gaattctcat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt
aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc
gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg
accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag
aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatcgg ggggggggtt
tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc tttgaaattt
ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt
ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca
actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag ctacatataa
ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga
aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt
agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga
tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt
actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc
gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc
aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct
tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg
tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga
catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga
tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg tctctacagg
atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga
gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa
ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat
ttaattatat cagttattac ccgggaatct cggtcgtaat gatttttata atgacgaaaa
aaaaaaaatt ggaaagaaaa cccccccccc gcagcgttgg gtcctggcca cgggtgcgca
tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct
gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
ccatgaacag aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc
ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga
gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct
ttaccgcagg tgggccattc tcatgaagaa tatcttgaat ttattgtcat attactagtt
ggtgtggaag tccatatatc ggtgatcaat atagtggttg acatgctggc tagtcaacat
tgagcctttt gatcatgcaa atatattacg gtattttaca atcaaatatc aaacttaact
attgacttta taacttattt aggtggtaac attcttataa aaaagaaaaa aattactgca
aaacagtact agcttttaac ttgtatccta ggttatctat gctgtctcac catagagaat
attacctatt tcagaatgta tgtccatgat tcgccgggta aatacatata atacacaaat
ctggcttaat aaagtctata atatatctca taaagaagtg ctaaattggc tagtgctata
tatttttaag aaaatttctt ttgactaagt ccatatcgac tttgtaaaag ttcactttag
catacatata ttacacgagc cagaaattgt aacttttgcc taaaatcaca aattgcaaaa
tttaattgct tgcaaaaggt cacatgctta taatcaactt ttttaaaaat ttaaaatact
tttttatttt ttatttttaa acataaatga aataatttat ttattgttta tgattaccga
aacataaaac ctgctcaaga aaaagaaact gttttgtcct tggaaaaaaa gcactaccta
ggagcggcca aaatgccgag gctttcatag cttaaactct ttacagaaaa taggcattat
agatcagttc gagttttctt attcttcctt ccggttttat cgtcacagtt ttacagtaaa
taagtatcac ctcttagagt taactatgag ataagcaagt atcatctcat ttcatttacc
tgaagtcgag taaacagaaa atccaattgt tgatgaacct caatgactta gaactatcta
tcggcagatc atataaagag gatttaggta cctagaggac tgtacctgga gtatatatat
atatatatat atattatctc aactatagtc catagaggtt tctttcttga ggccttaaac
tgctaaagaa tgatattggt ggaatgcaag caccaatctc tcttctttcg taactgttca
tatacttcaa accaagaatg taacgggcat tgacccatcc aaaaccttca gtagctgccc
ctttaaagtc agcaccttga ttaccgtatt ctgcttcaac acgatgagga tctgttcctc
ttgtgacatc atatttttca accacaatac cattataatc gacaaaagcc tttgtcatca
tgaaaagcca tctataagct agcctattcg ttacagttaa ataaccataa gaacggaggc
cttcccaagc aagaatttga tggggtgccc aaccaaatgg atagtcccat tgtctaattg
gtctcgaaat agaaattggg cctcgagaac gctccgtaca tgcagctaaa cctccaagca
tctctaactt gggtagtgct ttctccacca ttttctgtgc ttgctccttc gtggcaagtc
cagcccataa tgcccagaat gtagttgcgg attcgtatga cgttctgtgc ttgatttttg
tgttgtagtc aaagaaaaac cccgactcgt catcccacat atatttggta attgatgagg
caacgctaat tatcaacata tagattgtta tctatctgca tgaacacgaa atctttactt
gacgacttga ggctgatggt gtttatgcaa agaaaccact gtgtttaata tgtgtcactg
tttgatatta ctgtcagcgt agaagataat agtaaaagcg gttaataagt gtatttgaga
taagtgtgat aaagttttta cagcgaaaag acgataaata caagaaaatg attacgagga
tacggagaga ggtatgtaca tgtgtattta tatactaagc tgccggcggt tgtttgcaag
accgagaaaa ggctagcaag aatcgggtca ttgtagcgta tgcgcctgtg aacattctct
tcaacaagtt tgattccatt gcggtgaaat ggtaaaagtc aaccccctgc gatgtatatt
ttcctgtaca atcaatcaaa aagccaaatg atttagcatt atctttacat cttgttattt
tacagatttt atgtttagat cttttatgct tgcttttcaa aaggcctgca ggcaagtgca
caaacaatac ttaaataaat actactcagt aataacctat ttcttagcat ttttgacgaa
atttgctatt ttgttagagt cttttacacc atttgtctcc acacctccgc ttacatcaac
accaataacg ccatttaatc taagcgcatc accaacattt tctggcgtca gtccaccagc
taacataaaa tgtaagctct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
aggcgtatca cgaggccctt tcgtcttcaa
//