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ID   YEP13      preliminary; circular DNA; SYN; 10639 BP.
XX
AC   IG0128; ATCC37115;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector YEp13 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCV4 from pBR322 & pMB9-B1
RC   YEp13 from pCV4 & pYeleu10
RC   pCV13 from yeast 2 micron & LEU2 gene
RA   Broach J.R., Strathern J.N., Hicks J.B.;
RT   "Transformation in yeast: development of a hybrid cloning vector and
RT   isolation of the CAN1 gene";
RL   Gene 8:121-133(1979).
XX
RN   [2]
RC   pYeleu10 from ColE1 & LEU2 gene
RC   pYeleu11 from ColE1 & LEU2 gene
RC   pYeleu12 from ColE1 & LEU2 gene
RC   pYeleu17 from ColE1 & LEU2 gene
RC   pYehis1 from ColE1 & HIS2 gene
RC   pYehis2 from ColE1 & HIS2 gene
RC   pYehis3 from ColE1 & HIS2 gene
RA   Ratzkin B., Carbon J.;
RT   "Functional expression of cloned yeast DNA in Escherichia coli";
RL   Proc. Natl. Acad. Sci. U.S.A. 74:487-491(1977).
XX
RN   [3]
RC   pYE13 from pBR322 & yeast 2 micron & LEU2 gene
RA   Klar A., Strathern J., Broach J., Hicks J.;
RT   "Regulation of transcription in expressed and unexpressed mating
RT   type cassettes of yeast";
RL   Nature 289:239-244(1981).
XX
RN   [4]
RC   lambda gt-Schis from lambda & yeast HIS2 gene
RA   Struhl K., Cameron J.R., Davis R.W.;
RT   "Functional genetic expression of eukaryotic DNA in Escherichia
RT   coli";
RL   Proc. Natl. Acad. Sci. U.S.A. 73:1471-1475(1976).
XX
CC   YEp13 is mitotically stable in cir+ strains. [2]
CC   A YE type S. cerevisiae/Escherichia coli shuttle vector for cloning
CC   specific genes by transformation of yeast.  Transforms leu2- yeast to
CC   Leu+ at 10E3 to 10E4 colonies per microgram of DNA.
CC   An EcoRI fragment containing the 2 micron origin was cloned into the
CC   EcoRI site of pBR322.  The resulting plasmid was partially digested
CC   with PstI, and a PstI fragment containing yeast LEU2 cloned into the
CC   2 micron PstI site.
CC   Restriction digests analyzed on agarose gels give the following sizes
CC   (kb): PstI--5.5, 4.0, 0.9; HindIII--10.7. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YEp13)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli C600)(E.coli K802)(E.coli)(S.cerevisiae)(Saccharomyces)
CC   CP (yeast.2micron 30-200 copies/cell)
CC   CP (pMB1 25-80 copies/cell)
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(2micron plasmid B form)
CC   BR (YEp351)(YEp352)(YEp356)(YEp366)(YEp24)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. S. cerevisiae PstI-PstI 4043bp, LEU2 gene
FT                   2. yeast 2 micron B form EcoRI-EcoRI 2235bp
FT                   \ 2408..3942 & 703..2
FT                   \ UL = 3239bp 703..3942 US = 3079bp 3942..6318..703 A
FT                   \ UL = 3239bp 703..3942 US = 3079bp 703..6318..3942 B
FT                   PstI 2235bp, yeast 2 micron B form 2657..2657
FT                   -> plasmid 6278bp
FT                   1. plasmid EcoRI 6278bp 0..0
FT                   2. pBR322 EcoRI 4361bp 4360..4360
FT                   -> YEp13 10639bp"
FT   CDS             0..0
FT                   /note="ANT yeast CAN1 gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             86..1276
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             complement(3295..4155)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             complement(5975..7081)
FT                   /note="ANT yeast LEU2 gene"
FT   rep_origin      complement(9663..10013)
FT                   /note="ORI yeast 2 micron"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuII"
FT   misc_binding    0..0
FT                   /note="SIT unique XhoI"
XX
SQ   Sequence 10639 BP; 2300 A; 2069 C; 2001 G; 2402 T; 1867 other;
     agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga
     gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc
     acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc
     gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
     acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca
     atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg
     cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg tcctggccac
     gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
     ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
     cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
     aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
     ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
     tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
     accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
     tcattacccc catgaacaga aattccccct tacacggagg catcaagtga ccaaacagga
     aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
     actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
     tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     aggcgtatca cgaggccctt tcgtcttcaa gaattccacg gactatagac tatactagta
     tactccgtct actgtacgat acacttccgc tcaggtcctt gtcctttaac gaggccttac
     cactcttttg ttactctatt gatccagctc agcaaaggca gtgtgatcta agattctatc
     ttcgcgatgt agtaaaacta gctagaccga gaaagagact agaaatgcaa aaggcacttc
     tacaatggct gccatcatta ttatccgatg tgacgctgca gnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct cgaggagaac ttctagtata
     tccacatacc taatattatt gccttattaa aaatggaatc ccaacaatta catcaaaatc
     cacattctct tcaaaatcaa ttgtcctgta cttccttgtt catgtgtgtt caaaaacgtt
     atatttatag gataattata ctctatttct caacaagtaa ttggttgttt ggccgagcgg
     tctaaggcgc ctgattcaag aaatatcttg accgcagtta actgtgggaa tactcaggta
     tcgtaagatg caagagttcg aatctcttag caaccattat ttttttcctc aacataacga
     gaacacacag gggcgctatc gcacagaatc aaattcgatg actggaaatt ttttgttaat
     ttcagaggtc gcctgacgca tatacctttt tcaactgaaa aattgggaga aaaaggaaag
     gtgagaggcc ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg
     catcacaata cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg
     gttagcaatc gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat
     atatttcaag gatataccat tctaatgtct gcccctatgt ctgcccctaa gaagatcgtc
     gttttgccag gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa
     gctatttctg atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt
     gctgctatcg atgctacagg tgtcccactt ccagatgagg cgctggaagc ctccaagaag
     gttgatgccg ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga
     cctgaacaag gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca
     tgtaactttg catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa
     ggtactgact tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag
     gaagacgatg gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa
     agaatcacaa gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg
     tccttggata aagctaatgt tttggcctct tcaagattat ggagaaaaac tgtggaggaa
     accatcaaga acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc
     atgatcctag ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt
     ggtgatatca tctccgatga agcctccgtt atcccaggtt ccttgggttt gttgccatct
     gcgtccttgg cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac
     ggttctgctc cagatttgcc aaagaataag gttgacccta tcgccactat cttgtctgct
     gcaatgatgt tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca
     gttaaaaagg ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc
     accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct
     ctttttttat gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg
     aaattacaaa atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca
     tttatcaaga aggagaaaaa ggaggatagt aaaggaatac aggtaagcaa attgatacta
     atggctcaac gtgataagga aaaagaattg cactttaaca ttaatattga caaggaggag
     ggcaccacac aaaaagttag gtgtaacaga aaatcatgaa actacgattc ctaatttgat
     attggaggat tttctctaaa aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg
     accatttgat ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt
     ccctcaagaa ttttactctg tcagaaacgg ccttacgacg tagtgctgtc gacnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnctgcagt ctcaatgata ttcgaatacg ctttgaggag atacagccta atatccgaca
     aactgtttta cagatttacg atcgtacttg ttacccatca ttgaattttg aacatccgaa
     cctgggagtt ttccctgaaa cagatagtat atttgaacct gtataataat atatagtcta
     gcgctttacg gaagacaatg tatgtatttc ggttcctgga gaaactattg catctattgc
     ataggtaatc ttgcacgtcg catccccggt tcattttctg cgtttccatc ttgcacttca
     atagcatatc tttgttaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
     aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
     aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
     tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
     aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
     cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
     tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
     attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
     ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
     aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
     agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
     atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
     gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
     tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
     gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
     acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
     gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
     ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
     gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
     ctcatccttc aatgctatca tttcctttga tattggatca tatgcatagt accgagaaac
     tagtgcgaag tagtgatcag gtattgctgt tatctgatga gtatacgttg tcctggccac
     ggcagaagca cgcttatcgc tccaatttcc cacaacatta gtcaactccg ttaggccctt
     cattgaaaga aatgaggtca tcaaatgtct tccaatgtga gattttgggc cattttttat
     agcaaagatt gaataaggcg catttttctt caaagcttta ttgtacgatc tgactaagtt
     atcttttaat aattggtatt cctgtttatt gcttgaagaa ttgccggtcc tatttactcg
     ttttaggact ggttcagaa
//