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ID   YEP213     preliminary; circular DNA; SYN; 10667 BP.
XX
AC   U03499;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector YEp213 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-10667
RC   YEp213
RA   Stillman D.J.;
RT   ;
RL   Submitted (16-NOV-1993) by:
RL   Stillman D.J., Dept. of Cellular Viral and Molecular Biology,
RL   University of Utah Medical Center, Salt Lake City UT 84132 USA.
XX
CC   NCBI gi: 416341
CC   A general purpose episomal Saccharomyces cerevisiae/E.coli
CC   shuttle vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YEp213)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli K-12 r-m+)(Saccharomyces cerevisiae)(E.coli MM294)
CC   HO (E.coli C600)(S.cerevisiae SHY series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(2micron)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. yeast 2 micron EcoRI-EcoRI 2235bp
FT                   \ 2408..3942 & 703..2
FT                   \ UL = 3239bp 703..3942 US = 3079bp 3942..6318..703 A
FT                   \ UL = 3239bp 703..3942 US = 3079bp 703..6318..3942 B
FT                   3. yeast PstI-PstI 4043bp, LEU2 gene
FT                   -> YEp213 10667bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
XX
SQ   Sequence 10667 BP; 2837 A; 2459 C; 2350 G; 3021 T; 0 other;
     gaattctcat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt
     aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
     cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
     cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
     atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
     ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
     gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc
     gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg
     accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
     agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag
     aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt
     gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg
     cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
     tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt
     tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc
     gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat
     tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca
     caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct
     cgtttcatcg gtatcattac ccccatgaac agaaattccc ccttacacgg aggcatcaag
     tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattcc acggactata
     gactatacta gtatactccg tctactgtac gatacacttc cgctcaggtc cttgtccttt
     aacgaggcct taccactctt ttgttactct attgatccag ctcagcaaag gcagtgtgat
     ctaagattct atcttcgcga tgtagtaaaa ctagctagac cgagaaagag actagaaatg
     caaaaggcac ttctacaatg gctgccatca ttattatccg atgtgacgct gcagaagcag
     aaatacacgc ggtcagtgaa gctattccgc tattgaataa cctcagtcac cttgtgcaag
     aacttaacaa gaaaccaatt attaaaggct tacttactga tagtagatca acgatcagta
     taattaagtc tacaaatgaa gagaaattta gaaacagatt ttttggcaca aaggcaatga
     gacttagaga tgaagtatca ggtaataatt tatacgtata ctacatcgag accaagaaga
     acattgctga tgtgatgaca aaacctcttc cgataaaaac atttaaacta ttaactaaca
     aatggattca ttagatctat tacattatgg gtggtatgtt ggaataaaaa tcaactatca
     tctactaact agtatttacg ttactagtat attatcatat acggtgttag aagatgacgc
     aaatgatgag aaatagtcat ctaaattagt ggaagctgaa acgcaaggat tgataatgta
     ataggatcaa tgaatattaa catataaaat gatgataata atatttatag aattgtgtag
     aattgcagat tcccttttat ggattcctaa atcctcgact acgtcgttaa ggccgtttct
     gacagagtaa aattcttgag ggaactttca ccattatggg aaatggttca agaaggtatt
     gacttaaact ccatcaaatg gtcaggtcat tgagtgtttt ttatttgttg tatttttttt
     tttttagaga aaatcctcca atatataaat taggaatcat agtttcatga ttttctgtta
     cacctaactt tttgtgtggt gccctcctcc ttgtcaatat taatgttaaa gtgcaattct
     ttttccttat cacgttgagc cattagtatc aatttgctta cctgtattcc tttacatcct
     cctttttctc cttcttgata aatgtatgta gattgcgtat atagtttcgt ctaccctatg
     aacatattcc attttgtaat ttcgtgtcgt ttctattatg aatttcattt ataaagttta
     tgtacaaata tcataaaaaa agagaatctt tttaagcaag gattttctta acttcttcgg
     cgacagcatc accgacttcc gtggtactgt tggaaccacc taaatcacca gttctgatac
     ctgcatccaa aaccttttta actgcatctt caatggcctt accttcttca ggcaagttca
     atgacaattt caacatcatt gcagcagaca agatagtggc gatagggttg accttattct
     ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt
     ctggcaaaga ggccaaggac gcagatggca acaaacccaa ggaacctggg ataacggagg
     cttcatcgga gatgatatca ccaaacatgt tgctggtgat tataatacca tttaggtggg
     ttgggttctt aactaggatc atggcggcag aatcaatcaa ttgatgttga accttcaatg
     tagggaattc gttcttgatg gtttcctcca cagtttttct ccataatctt gaagaggcca
     aaacattagc tttatccaag gaccaaatag gcaatggtgg ctcatgttgt agggccatga
     aagcggccat tcttgtgatt ctttgcactt ctggaacggt gtattgttca ctatcccaag
     cgacaccatc accatcgtct tcctttctct taccaaagta aatacctccc actaattctc
     tgacaacaac gaagtcagta cctttagcaa attgtggctt gattggagat aagtctaaaa
     gagagtcgga tgcaaagtta catggtctta agttggcgta caattgaagt tctttacgga
     tttttagtaa accttgttca ggtctaacac taccggtacc ccatttagga ccacccacag
     cacctaacaa aacggcatca accttcttgg aggcttccag cgcctcatct ggaagtggga
     cacctgtagc atcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat
     tggaacgaac atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac
     caacgtggtc acctggcaaa acgacgatct tcttaggggc agacattaga atggtatatc
     cttgaaatat atatatatat attgctgaaa tgtaaaaggt aagaaaagtt agaaagtaag
     acgattgcta accacctatt ggaaaaaaca ataggtcctt aaataatatt gtcaacttca
     agtattgtga tgcaagcatt tagtcatgaa cgcttctcta ttctatatga aaagccggtt
     ccgcggctct cacctttcct ttttctccca atttttcagt tgaaaaaggt atatgcgtca
     ggcgacctct gaaattaaca aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg
     cccctgtgtg ttctcgttat gttgaggaaa aaaataatgg ttgctaagag attcgaactc
     ttgcatctta cgatacctga gtattcccac agttaactgc ggtcaagata tttcttgaat
     caggcgcctt agaccgctcg gccaaacaac caattacttg ttgagaaata gagtataatt
     atcctataaa tataacgttt ttgaacacac atgaacaagg aagtacagga caattgattt
     tgaagagaat gtggattttg atgtaattgt tgggattcca tttttaataa ggcaataata
     ttaggtatgt agatatacta gaagttctcc tcgacctcct cttcagtact aaatctacca
     ataccaaatc tgatggaaga atgggctaat gcatcatcct tacccagcgc atgtaaaaca
     taagaaggtt ctagggaagc agatgtacag gctgaacccg aggataatgc gatatccctt
     agtgccatca ataaagattc tccttccacg taggcgaaag aaacgttaac acaccctgga
     taacgatgat ctggagatcc gttcaacgtg gtatgttcag cggataatag acctttgact
     aatttatcgg atagtctttt gatgtgagct tggtcgttgt caaattcttt cttcatcaat
     ctcgcagctt caccaaatcc cgctaccaat gggggggcca aagtaccaga tctcaatcct
     ctctcttggc caccaccgga tagtaaaggt tctaatctaa ctcttggtct ccttcttaca
     tagatggcac ctattccctt tggaccgtaa atcttgtgag aagaaattga tagtaaatca
     atgttcattt cattgacatc aatgtgaatc ttaccatagg cttgtgcggc gtcagtatga
     aagtagatct tattctttct acaaattgca ccaatttctt taataggttg aatgacaccg
     atttcattat tgacagccat cacagagacg agacaggtat ctggtctaat ggcatcttcc
     aattccttca aatcgataag accttgatcg tccacattta ggaaagtgac ttcaaatccc
     tccttcatca tggcccgtgc ggcttccaag acacacttgt gttccgttct agtggtgatg
     atgtgtttct tagtcttctt ataaaatctt gggacaccct taagaaccat attattagat
     tcggtcgctc ccgaagtgaa tattatttcc ttggggtcgg cattgatcat ctttgctacg
     taagctctag cattttccac agcagtattt gtttcccaac cgtaagagtg agtgttggaa
     tgaggattac cataaagtcc cgtataaaac ttcaacatcg tatccaaaac cctagggtct
     gttggtgtag tggcttgcat gtcaagatat atgggacgag taccaaaacc tgtgttttct
     tgataagcat ggctcattgc agtgctacca gaagctacta cagcatctgg ggtggtaccg
     gatgcactcg cacgggcact agcctgtgcc tttgcagcag cctgaatatc ggtatgcgtt
     tccagagaga agttgtcgtc taacttcacg cctgctgcag tctcaatgat attcgaatac
     gctttgagga gatacagcct aatatccgac aaactgtttt acagatttac gatcgtactt
     gttacccatc attgaatttt gaacatccga acctgggagt tttccctgaa acagatagta
     tatttgaacc tgtataataa tatatagtct agcgctttac ggaagacaat gtatgtattt
     cggttcctgg agaaactatt gcatctattg cataggtaat cttgcacgtc gcatccccgg
     ttcattttct gcgtttccat cttgcacttc aatagcatat ctttgttaac gaagcatctg
     tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
     tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa
     tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca
     aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa
     caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata
     actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc
     ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg
     tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat
     actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg
     gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt
     ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag taatactaga
     gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga
     tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa
     tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg
     gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta
     tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg
     cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat
     ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt
     aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg
     atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt
     ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc atttcctttg
     atattggatc atatgcatag taccgagaaa ctagtgcgaa gtagtgatca ggtattgctg
     ttatctgatg agtatacgtt gtcctggcca cggcagaagc acgcttatcg ctccaatttc
     ccacaacatt agtcaactcc gttaggccct tcattgaaag aaatgaggtc atcaaatgtc
     ttccaatgtg agattttggg ccatttttta tagcaaagat tgaataaggc gcatttttct
     tcaaagcttt attgtacgat ctgactaagt tatcttttaa taattggtat tcctgtttat
     tgcttgaaga attgccggtc ctatttactc gttttaggac tggttca
//