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ID   YEP24      preliminary; circular DNA; SYN; 7769 BP.
XX
AC   L09156; VB0067; IG0322; ATCC37051;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector YEp24 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRB101 from pBR322 & URA3 gene
RC   pRB43 from pBR322 & ura3-3 gene
RA   Rose M., Grisafi P., Botstein D.;
RT   "Structure and function of the yeast URA3 gene: expression in
RT   Escherichia coli";
RL   Gene 29:113-124(1984).
XX
RN   [2]
RP   1-7769
RC   YEp24 from pBR322 & yeast 2 micron & URA3 fragment
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
RN   [3]
RP   1-7769
RC   YEp24
RA   ;
RT   ;
RL   Submitted (12-SEP-1986) on magnetic tape by:
RL   New England Biolabs.
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   The tetracycline resistance promoter was separated from coding
CC   sequence by the URA3 gene. Tc-R is dependent on the construct.
CC   ATCC size is 7769 bp.
CC   This was constructed by inserting a 2.2 kb EcoRI 2 micron B fragment
CC   and a 1.1 kb HindIII URA3 fragment into the EcoRI and HindIII sites
CC   of pBR322. [7]
CC   A general purpose episomal Saccharomyces cerevisiae/Escherichia coli
CC   shuttle vector. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YEp24)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (NEB)(ATCC)
CC   HO (E.coli K-12 r-m+)(E.coli C600)(E.coli MM294)(E.coli)
CC   HO (S.cerevisiae)(S.cerevisiae SHY series)
CC   CP (high)
CC   FN (expression)
CC   SE ()
CC   PA (2micron)(pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 EcoRI 4361bp 4360..4360
FT                   2. yeast 2 micron EcoRI-EcoRI 2235bp
FT                   \ 2408..3942 & 703..2
FT                   \ UL = 3239bp 703..3942 US = 3079bp 3942..6318..703 A
FT                   \ UL = 3239bp 703..3942 US = 3079bp 703..6318..3942 B
FT                   -> plasmid 6595bp
FT                   1. plasmid HindIII 6595bp 30..30
FT                   2. yeast HindIII-HindIII 1166bp, URA3 gene
FT                   -> YEp24 7769bp"
FT   rep_origin      1..2247
FT                   /note="ORI yeast 2 micron"
FT   misc_binding    0..0
FT                   /note="SIT unique BclI"
FT   misc_binding    0..0
FT                   /note="SIT unique MunI"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI"
FT   misc_binding    0..0
FT                   /note="SIT unique SnaBI"
FT   misc_binding    0..0
FT                   /note="SIT unique HpaI"
FT   misc_binding    0..0
FT                   /note="SIT unique Bpu1102I"
FT   misc_binding    0..0
FT                   /note="SIT unique ClaI"
FT   misc_binding    0..0
FT                   /note="SIT unique Sse8387I"
FT   CDS             2499..3302
FT                   /note="ANT yeast URA3 gene (uracil)"
FT   misc_binding    0..0
FT                   /note="SIT unique NcoI"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI"
FT   misc_binding    0..0
FT                   /note="SIT unique SmaI"
FT   CDS             3495..4685
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    0..0
FT                   /note="SIT unique NheI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique SgrAI"
FT   misc_binding    0..0
FT                   /note="SIT unique SphI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoNI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   misc_binding    0..0
FT                   /note="SIT unique EagI"
FT   misc_binding    0..0
FT                   /note="SIT unique BspEI"
FT   misc_binding    0..0
FT                   /note="SIT unique BsaBI"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuII"
FT   misc_binding    0..0
FT                   /note="SIT unique Tth111I"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             6707..7495
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique AseI"
FT   misc_binding    0..0
FT                   /note="SIT unique AatI"
XX
SQ   Sequence 7769 BP; 2102 A; 1830 C; 1844 G; 1993 T; 0 other;
     gaattctgaa ccagtcctaa aacgagtaaa taggaccggc aattcttcaa gcaataaaca
     ggaataccaa ttattaaaag ataacttagt cagatcgtac aataaagctt tgaagaaaaa
     tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg gaagacattt
     gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg tgggaaattg
     gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga taacagcaat
     acctgatcac tacttcgcac tagtttctcg gtactatgca tatgatccaa tatcaaagga
     aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct
     aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga
     ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata
     aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg
     tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt
     tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac
     gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca
     aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct
     ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta
     ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata
     cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat
     aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca
     atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag
     cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa
     tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag
     agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa
     aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt
     aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt
     gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg
     ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt
     tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc
     ttcgttaaca aagatatgct attgaagtgc aagatggaaa cgcagaaaat gaaccgggga
     tgcgacgtgc aagattacct atgcaataga tgcaatagtt tctccaggaa ccgaaataca
     tacattgtct tccgtaaagc gctagactat atattattat acaggttcaa atatactatc
     tgtttcaggg aaaactccca ggttcggatg ttcaaaattc aatgatgggt aacaagtacg
     atcgtaaatc tgtaaaacag tttgtcggat attaggctgt atctcctcaa agcgtattcg
     aatatcattg agaagctgca gcgtcacatc ggataataat gatggcagcc attgtagaag
     tgccttttgc atttctagtc tctttctcgg tctagctagt tttactacat cgcgaagata
     gaatcttaga tcacactgcc tttgctgagc tggatcaata gagtaacaaa agagtggtaa
     ggcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac ggagtatact
     agtatagtct atagtccgtg gaattctcat gtttgacagc ttatcatcga taagcttttc
     aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt
     ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg
     tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac
     tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg
     aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa
     agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag
     ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt
     tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac
     tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg
     gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag
     gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt
     tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta
     ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca
     tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg
     acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat
     ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg
     gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact
     aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt
     aattatatca gttattaccc gggaatctcg gtcgtaatga tttttataat gacgaaaaaa
     aaaaaattgg aaagaaaaag ctttaatgcg gtagtttatc acagttaaat tgctaacgca
     gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc
     tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg
     tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc
     aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc
     tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc
     tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag
     ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
     agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
     tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
     ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
     ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca
     cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg
     ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa
     tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc
     gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg
     ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac
     tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac
     gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga
     aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg
     gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat
     ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa
     ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat
     cattaccccc atgaacagaa attccccctt acacggaggc atcaagtgac caaacaggaa
     aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
     ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
     gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     ggcgtatcac gaggcccttt cgtcttcaa
//