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ID YEP24 preliminary; circular DNA; SYN; 7769 BP.
XX
AC L09156; VB0067; IG0322; ATCC37051;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Saccharomyces/E.coli plasmid vector YEp24 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pRB101 from pBR322 & URA3 gene
RC pRB43 from pBR322 & ura3-3 gene
RA Rose M., Grisafi P., Botstein D.;
RT "Structure and function of the yeast URA3 gene: expression in
RT Escherichia coli";
RL Gene 29:113-124(1984).
XX
RN [2]
RP 1-7769
RC YEp24 from pBR322 & yeast 2 micron & URA3 fragment
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
XX
RN [3]
RP 1-7769
RC YEp24
RA ;
RT ;
RL Submitted (12-SEP-1986) on magnetic tape by:
RL New England Biolabs.
XX
CC These data and their annotation were supplied to GenBank by Will
CC Gilbert under the auspices of the GenBank Currator Program.
CC
CC The tetracycline resistance promoter was separated from coding
CC sequence by the URA3 gene. Tc-R is dependent on the construct.
CC ATCC size is 7769 bp.
CC This was constructed by inserting a 2.2 kb EcoRI 2 micron B fragment
CC and a 1.1 kb HindIII URA3 fragment into the EcoRI and HindIII sites
CC of pBR322. [7]
CC A general purpose episomal Saccharomyces cerevisiae/Escherichia coli
CC shuttle vector. [1]
CC Medium is 1227 LB plus ampicillin.
CC NM (YEp24)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (NEB)(ATCC)
CC HO (E.coli K-12 r-m+)(E.coli C600)(E.coli MM294)(E.coli)
CC HO (S.cerevisiae)(S.cerevisiae SHY series)
CC CP (high)
CC FN (expression)
CC SE ()
CC PA (2micron)(pBR322)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 EcoRI 4361bp 4360..4360
FT 2. yeast 2 micron EcoRI-EcoRI 2235bp
FT \ 2408..3942 & 703..2
FT \ UL = 3239bp 703..3942 US = 3079bp 3942..6318..703 A
FT \ UL = 3239bp 703..3942 US = 3079bp 703..6318..3942 B
FT -> plasmid 6595bp
FT 1. plasmid HindIII 6595bp 30..30
FT 2. yeast HindIII-HindIII 1166bp, URA3 gene
FT -> YEp24 7769bp"
FT rep_origin 1..2247
FT /note="ORI yeast 2 micron"
FT misc_binding 0..0
FT /note="SIT unique BclI"
FT misc_binding 0..0
FT /note="SIT unique MunI"
FT misc_binding 0..0
FT /note="SIT unique XbaI"
FT misc_binding 0..0
FT /note="SIT unique SnaBI"
FT misc_binding 0..0
FT /note="SIT unique HpaI"
FT misc_binding 0..0
FT /note="SIT unique Bpu1102I"
FT misc_binding 0..0
FT /note="SIT unique ClaI"
FT misc_binding 0..0
FT /note="SIT unique Sse8387I"
FT CDS 2499..3302
FT /note="ANT yeast URA3 gene (uracil)"
FT misc_binding 0..0
FT /note="SIT unique NcoI"
FT misc_binding 0..0
FT /note="SIT unique ApaI"
FT misc_binding 0..0
FT /note="SIT unique SmaI"
FT CDS 3495..4685
FT /note="ANT E. coli tetracycline resistance gene (tet)"
FT misc_binding 0..0
FT /note="SIT unique NheI"
FT misc_binding 0..0
FT /note="SIT unique BamHI"
FT misc_binding 0..0
FT /note="SIT unique SgrAI"
FT misc_binding 0..0
FT /note="SIT unique SphI"
FT misc_binding 0..0
FT /note="SIT unique EcoNI"
FT misc_binding 0..0
FT /note="SIT unique SalI"
FT misc_binding 0..0
FT /note="SIT unique EagI"
FT misc_binding 0..0
FT /note="SIT unique BspEI"
FT misc_binding 0..0
FT /note="SIT unique BsaBI"
FT misc_binding 0..0
FT /note="SIT unique PvuII"
FT misc_binding 0..0
FT /note="SIT unique Tth111I"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 6707..7495
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 0..0
FT /note="SIT unique AseI"
FT misc_binding 0..0
FT /note="SIT unique AatI"
XX
SQ Sequence 7769 BP; 2102 A; 1830 C; 1844 G; 1993 T; 0 other;
gaattctgaa ccagtcctaa aacgagtaaa taggaccggc aattcttcaa gcaataaaca
ggaataccaa ttattaaaag ataacttagt cagatcgtac aataaagctt tgaagaaaaa
tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg gaagacattt
gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg tgggaaattg
gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga taacagcaat
acctgatcac tacttcgcac tagtttctcg gtactatgca tatgatccaa tatcaaagga
aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct
aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga
ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata
aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg
tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt
tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac
gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca
aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct
ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta
ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata
cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat
aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca
atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag
cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa
tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag
agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa
aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt
aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt
gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg
ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt
tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc
ttcgttaaca aagatatgct attgaagtgc aagatggaaa cgcagaaaat gaaccgggga
tgcgacgtgc aagattacct atgcaataga tgcaatagtt tctccaggaa ccgaaataca
tacattgtct tccgtaaagc gctagactat atattattat acaggttcaa atatactatc
tgtttcaggg aaaactccca ggttcggatg ttcaaaattc aatgatgggt aacaagtacg
atcgtaaatc tgtaaaacag tttgtcggat attaggctgt atctcctcaa agcgtattcg
aatatcattg agaagctgca gcgtcacatc ggataataat gatggcagcc attgtagaag
tgccttttgc atttctagtc tctttctcgg tctagctagt tttactacat cgcgaagata
gaatcttaga tcacactgcc tttgctgagc tggatcaata gagtaacaaa agagtggtaa
ggcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac ggagtatact
agtatagtct atagtccgtg gaattctcat gtttgacagc ttatcatcga taagcttttc
aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt
ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg
tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac
tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg
aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa
agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag
ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt
tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac
tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg
gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag
gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt
tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta
ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca
tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg
acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat
ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg
gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact
aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt
aattatatca gttattaccc gggaatctcg gtcgtaatga tttttataat gacgaaaaaa
aaaaaattgg aaagaaaaag ctttaatgcg gtagtttatc acagttaaat tgctaacgca
gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc
tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg
tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc
aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc
tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc
tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag
ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca
cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg
ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa
tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc
gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg
ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac
tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac
gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga
aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg
gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat
ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa
ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat
cattaccccc atgaacagaa attccccctt acacggaggc atcaagtgac caaacaggaa
aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
ggcgtatcac gaggcccttt cgtcttcaa
//