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ID YEP351 preliminary; circular DNA; SYN; 5644 BP.
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AC L14757; ATCC37672;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Saccharomyces/E.coli plasmid vector YEp351 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC YEp351 from pUC18 & pC4B, yeast 2 micron/LEU2 gene
RC YEp352 from pUC18 & yeast 2 micron & yeast URA3 gene
RC YIp351 from YEp351
RC YIp352 from YEp352
RA Hill J.E., Myers A.M., Koerner T.J., Tzagoloff A.;
RT "Yeast/E. coli shuttle vectors with multiple unique restriction
RT sites";
RL Yeast 2:163-167(1986).
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CC pUC18 was digested with NdeI and blunt-ended by filling in. A 2.95
CC BamHI/XbaI fragment of pC4B was blunt-ended (filled in) and ligated to
CC the pUC18.
CC The 2.95 kb fragment of pC4B includes the LEU2 gene (nt 286-2256 in
CC the final construct, where the A of the ATG of lacZ' is number 1) and
CC the 2 micron replication system (nt 2257-3241).
CC Restriction digests of the clone give the following sizes (kb):
CC BamHI--6.0; XbaI--6.0; PstI--6.0; HindIII--6.0. (ATCC staff)
CC This is a E.coli/yeast episomal shuttle vector with multiple
CC restriction sites for cloning. (personal communication)
CC Medium is 1227 LB plus ampicillin.
CC NCBI gi: 295270
CC NM (YEp351)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli RR1)(S.cerevisiae)(E.coli)(Saccharomyces)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pBR322)(2micron)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. yeast 2 micron 6318bp
FT 2. pYe(Leu2)10 SalI-XhoI 2200bp, LEU2 gene
FT -> pC4B 8500bp
FT 1. pUC18 NdeI 2686bp 185..185, MCS
FT Klenow
FT 2. pC4B BamHI-XbaI 2955bp 286..3241, LEU2 gene/2u ori
FT Klenow:Klenow
FT -> YEp351 5644bp"
FT misc_binding 0..0
FT /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-
FT PstI-SphI-HindIII"
FT misc_binding 0..0
FT /note="SIT unique SacI-SmaI-BamHI-XbaI-SalI-PstI-
FT SphI-HindIII"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI yeast 2 micron"
FT promoter 0..0
FT /note="PRO E. coli lac gene"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="ANT yeast LEU2 gene"
FT misc_feature 1..285
FT /note="from Escherichia coli"
FT misc_feature 286..3441
FT /note="from Saccharomyces cerevisiae"
FT misc_feature 3442..5644
FT /note="from Escherichia coli"
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SQ Sequence 5644 BP; 1520 A; 1259 C; 1241 G; 1624 T; 0 other;
atgaccatga ttacgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg
catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
acccaactta atcgccttgc agcacatccc cccttcgcca gctggcgtaa tagcgaagag
gcccgcaccg atcgcccttc ccaacagttg cgtagcctga atggcgaatg gcgcctgatg
cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagatcc ggtacgtcgt
taaggccgtt tctgacagag taaaattctt gagggaactt tcaccattat ggaaaatggt
tcaagaaggt attgacttaa actccatcaa atggtcaggt catttgagtg ttttttattt
gttgtatttt ttttttttta gagaaaatcc tccaatatca aattagggat tcgtagtttc
atgattttct gttataccta actttttgtg tggtgccctc ctccttgtca atattaatgt
taaagtgcaa ttctctttcc ttatcacgtt gagccattag tatcaatttg cttacctgta
ttcctttagc tatcctcctt tttctccttc ttgataaatg tatgtagatt gcgtttgtct
accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat
aaagtttatg tacaaatatc ataaaaaaga gaatcttttt aagcaaggat tttcttaact
tcttcggcga cagcatcacc gacttcggtg gtactgttgg aaccacctaa atcaccagtt
ctgatacctg catccaaaac ctttttaact gcatcttcaa tggccttacc ttcttcaggc
aagttcaatg acaatttcaa catcattgca gcagacaaga tagtggcgat agggtcaacc
ttattcttgg caaatccggg agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg
ttcttgtctg gcaaagaggc caaggacgca gatggcaaca aacccaagga acctggataa
cgtaaggctt catcggagat gatatcacca aacatgttgc tggtgattat aataccattt
aggtgggttg ggttcttaac taggatcatg gcggcagaat caatcaattg atgttgaacc
ttcaatgtag ggaattcgtt cttgatggtt tcctccacag tttttctcca taatcttgaa
gaggccaaaa cattagcttt atccaaggtc caaataggca atggtggctc atgttgtagg
gccatgaaag cggccattct tgtgattctt tgcacttctg gaacggtgta ttgttcacta
tcccaagcga caccatcacc atcgtcttcc tttctcttac caaagtaaat acctcccact
aattctctga caacaacgaa gtcagtacct ttagcaaatt gtggcttgat tggagataag
tctaaaagag agtcggatgc aaagttacat ggtcttaagt tggcgtacaa ttgaagttct
ttacggattt ttagtaaacc ttgttcaggt ctaacactac cggtacccca ttttggacca
cccacagcac ctaacaaaac ggcatcaacc ttcttggagg cttccagtgc ctcatctgga
agtgggacac ctgtagcatc gatagcagca ccaccaatta aatgattttc gaaatcgaac
ttgacattgg aacgaacatc agaaatagct ttaagaacct taatggcttc ggctgtgatt
tcttgaccaa cgtggtcacc tggcaaaacg acgatcttct taggaacaaa cattagaatg
gtatatcctt gaaatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa
gtaagacgat tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa
cttcaagtat tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc
cggttccggc ctctcacctt tcctttttct cccaattttt cagttgaaaa aggtatatgc
gtcaggcgac ctctgaaatt aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat
agcgcccctg tgtgttctcg ttatgttgag gaaaaaaata atggttgcta agagattcga
actcttgcat cttacgatac ctgagtattc ccacagttaa cgaagcatct gtgcttcatt
ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta
tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc
ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc
actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa
aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt
tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga
acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct
attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca
ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat
aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt
tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg
aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga
aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta
gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
cgttggccga ttcattaatc cagctggcac gacaggtttc ccgactggaa agcgggcagt
gagcgcaacg caattaatgt gagttacctc actcattagg caccccaggc tttacacttt
atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
agct
//