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ID   YEP351     preliminary; circular DNA; SYN; 5644 BP.
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AC   L14757; ATCC37672;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YEp351 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   YEp351 from pUC18 & pC4B, yeast 2 micron/LEU2 gene
RC   YEp352 from pUC18 & yeast 2 micron & yeast URA3 gene
RC   YIp351 from YEp351
RC   YIp352 from YEp352
RA   Hill J.E., Myers A.M., Koerner T.J., Tzagoloff A.;
RT   "Yeast/E. coli shuttle vectors with multiple unique restriction
RT   sites";
RL   Yeast 2:163-167(1986).
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CC   pUC18 was digested with NdeI and blunt-ended by filling in.  A 2.95
CC   BamHI/XbaI fragment of pC4B was blunt-ended (filled in) and ligated to
CC   the pUC18.
CC   The 2.95 kb fragment of pC4B includes the LEU2 gene (nt 286-2256 in
CC   the final construct, where the A of the ATG of lacZ' is number 1) and
CC   the 2 micron replication system (nt 2257-3241).
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--6.0; XbaI--6.0; PstI--6.0; HindIII--6.0. (ATCC staff)
CC   This is a E.coli/yeast episomal shuttle vector with multiple
CC   restriction sites for cloning. (personal communication)
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 295270
CC   NM (YEp351)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(S.cerevisiae)(E.coli)(Saccharomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(2micron)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. yeast 2 micron 6318bp
FT                   2. pYe(Leu2)10 SalI-XhoI 2200bp, LEU2 gene
FT                   -> pC4B 8500bp
FT                   1. pUC18 NdeI 2686bp 185..185, MCS
FT                   Klenow
FT                   2. pC4B BamHI-XbaI 2955bp 286..3241, LEU2 gene/2u ori
FT                   Klenow:Klenow
FT                   -> YEp351 5644bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-
FT                   PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique SacI-SmaI-BamHI-XbaI-SalI-PstI-
FT                   SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
FT   misc_feature    1..285
FT                   /note="from Escherichia coli"
FT   misc_feature    286..3441
FT                   /note="from Saccharomyces cerevisiae"
FT   misc_feature    3442..5644
FT                   /note="from Escherichia coli"
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SQ   Sequence 5644 BP; 1520 A; 1259 C; 1241 G; 1624 T; 0 other;
     atgaccatga ttacgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg
     catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     acccaactta atcgccttgc agcacatccc cccttcgcca gctggcgtaa tagcgaagag
     gcccgcaccg atcgcccttc ccaacagttg cgtagcctga atggcgaatg gcgcctgatg
     cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagatcc ggtacgtcgt
     taaggccgtt tctgacagag taaaattctt gagggaactt tcaccattat ggaaaatggt
     tcaagaaggt attgacttaa actccatcaa atggtcaggt catttgagtg ttttttattt
     gttgtatttt ttttttttta gagaaaatcc tccaatatca aattagggat tcgtagtttc
     atgattttct gttataccta actttttgtg tggtgccctc ctccttgtca atattaatgt
     taaagtgcaa ttctctttcc ttatcacgtt gagccattag tatcaatttg cttacctgta
     ttcctttagc tatcctcctt tttctccttc ttgataaatg tatgtagatt gcgtttgtct
     accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat
     aaagtttatg tacaaatatc ataaaaaaga gaatcttttt aagcaaggat tttcttaact
     tcttcggcga cagcatcacc gacttcggtg gtactgttgg aaccacctaa atcaccagtt
     ctgatacctg catccaaaac ctttttaact gcatcttcaa tggccttacc ttcttcaggc
     aagttcaatg acaatttcaa catcattgca gcagacaaga tagtggcgat agggtcaacc
     ttattcttgg caaatccggg agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg
     ttcttgtctg gcaaagaggc caaggacgca gatggcaaca aacccaagga acctggataa
     cgtaaggctt catcggagat gatatcacca aacatgttgc tggtgattat aataccattt
     aggtgggttg ggttcttaac taggatcatg gcggcagaat caatcaattg atgttgaacc
     ttcaatgtag ggaattcgtt cttgatggtt tcctccacag tttttctcca taatcttgaa
     gaggccaaaa cattagcttt atccaaggtc caaataggca atggtggctc atgttgtagg
     gccatgaaag cggccattct tgtgattctt tgcacttctg gaacggtgta ttgttcacta
     tcccaagcga caccatcacc atcgtcttcc tttctcttac caaagtaaat acctcccact
     aattctctga caacaacgaa gtcagtacct ttagcaaatt gtggcttgat tggagataag
     tctaaaagag agtcggatgc aaagttacat ggtcttaagt tggcgtacaa ttgaagttct
     ttacggattt ttagtaaacc ttgttcaggt ctaacactac cggtacccca ttttggacca
     cccacagcac ctaacaaaac ggcatcaacc ttcttggagg cttccagtgc ctcatctgga
     agtgggacac ctgtagcatc gatagcagca ccaccaatta aatgattttc gaaatcgaac
     ttgacattgg aacgaacatc agaaatagct ttaagaacct taatggcttc ggctgtgatt
     tcttgaccaa cgtggtcacc tggcaaaacg acgatcttct taggaacaaa cattagaatg
     gtatatcctt gaaatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa
     gtaagacgat tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa
     cttcaagtat tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc
     cggttccggc ctctcacctt tcctttttct cccaattttt cagttgaaaa aggtatatgc
     gtcaggcgac ctctgaaatt aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat
     agcgcccctg tgtgttctcg ttatgttgag gaaaaaaata atggttgcta agagattcga
     actcttgcat cttacgatac ctgagtattc ccacagttaa cgaagcatct gtgcttcatt
     ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
     catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
     ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta
     tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc
     ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc
     actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa
     aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt
     tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga
     acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct
     attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca
     ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat
     aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt
     tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg
     aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga
     aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta
     gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     cgttggccga ttcattaatc cagctggcac gacaggtttc ccgactggaa agcgggcagt
     gagcgcaacg caattaatgt gagttacctc actcattagg caccccaggc tttacacttt
     atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     agct
//