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ID   YEP353     preliminary; circular DNA; SYN; 7944 BP.
XX
AC   U03500; ATCC37725;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector YEp353 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-7944
RC   YEp352E from YEp352 & linker
RC   YEp363A from pNM480 & YEp351
RC   YEp353A from pNM480 & YEp352
RC   YEp353 from YEp353A & YEp352E
RC   YEp354A from pNM481 & YEp352
RC   YEp354 from YEp354A & YEp352E
RC   YEp355A from pNM482 & YEp352
RC   YEp355 from YEp355A & YEp352E
RC   YEp356, YEp356R from YEp353 & pUC18
RC   YEp357, YEp357R from YEp354 & pUC18
RC   YEp358, YEp358R from YEp355 & pUC18
RC   YEp363 from YEp363A & YEp353
RC   YEp364 from YEp363A & YEp354
RC   YEp365 from YEp363A & YEp355
RC   YEp366 from YEp363A & YEp356
RC   YEp367 from YEp363A & YEp357
RC   YEp368 from YEp363A & YEp358
RC   YEp366R from YEp363A & YEp356R
RC   YEp367R from YEp363A & YEp357R
RC   YEp368R from YEp363A & YEp358R
RC   YIp353 from YEp353 & YIp352
RC   YIp354 from YEp354 & YIp352
RC   YIp355 from YEp355 & YIp352
RC   YIp356 from YEp356 & YIp352
RC   YIp357 from YEp357 & YIp352
RC   YIp358 from YEp358 & YIp352
RC   YIp356R from YEp356R & YIp352
RC   YIp357R from YEp357R & YIp352
RC   YIp358R from YEp358R & YIp352
RC   YIp363 from YEp363 & YIp351
RC   YIp364 from YEp364 & YIp351
RC   YIp365 from YEp365 & YIp351
RC   YIp366 from YEp366 & YIp351
RC   YIp367 from YEp367 & YIp351
RC   YIp368 from YEp368 & YIp351
RC   YIp366R from YEp366R & YIp351
RC   YIp367R from YEp367R & YIp351
RC   YIp368R from YEp368R & YIp351
RA   Myers A.M., Tzagoloff A., Kinney D.M., Lusty C.J.;
RT   "Yeast shuttle and integrative vectors with multiple cloning sites
RT   suitable for construction of lacZ fusions";
RL   Gene 45:299-310(1986).
XX
RN   [2]
RP   1-7944
RC   YEp353
RA   Stillman D.J.;
RT   ;
RL   Submitted (16-NOV-1993) by:
RL   Stillman D.J., Dept. of Cellular, Viral and Molecular Biology,
RL   University of Utah Medical Center, Salt Lake City UT 84132 USA.
XX
CC   NCBI gi: 416342
CC   The sequence and reading frame of the multiple cloning sequence is:
CC   5' GA ATT CCC GGG GAT CCG TCG ACC TGC AGC CAA GCT TGC GAT CCC 3'.
CC   The sequence begins with the first nucleotide of the MCS & ends with
CC   the CCC for amino acid 8 of beta-galactosidase.
CC   One of 3 promoter-cloning YE type shuttle vectors (ATCC 37725 - 37727)
CC   with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase
CC   reporter gene and multiple cloning sites differing in reading frame.
CC   The cleavage position in the reading frame for cloning sites is (where
CC   3 = between triplets): EcoRI-2; SmaI-3; BamHI-3; SalI-3; PstI-1;
CC   HindIII-2.
CC   Restriction digests of the clone give the following sizes (kb):
CC   PstI--8.0; EcoRI--8.0; BamHI--8.0; HindIII--8.0. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YEp353)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(E.coli MC1061)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(2micron)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp352 remove PvuII-PvuII 322bp
FT                   \ 5021..5181..162, lacZ'/lacI genes/4859bp
FT                   S1 nuclease, remove 6bp ATG 5' to PvuII 6bp
FT                   2. EcoRI linker 12bp cccggattcggg
FT                   -> YEp352E 4870bp
FT                   1. pNM480 EcoRI-DraI 3150bp
FT                   2. YEp352 remove EcoRI-NarI 216bp 16..232,
FT                   \ 4966bp [215bp]
FT                   -> YEp353A 8100bp
FT                   1. YEp353A EcoRI-NcoI 3900bp, MCS/lacZ/URA3
FT                   2. YEp352E large EcoRI-NcoI 4063bp, YEp352 958..5021
FT                   -> YEp353 7944bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   reporter gene"
XX
SQ   Sequence 7944 BP; 1981 A; 1955 C; 1935 G; 2073 T; 0 other;
     gaattcccgg ggatccgtcg acctgcagcc aagcttgcga tcccgccgtc gttttacaac
     gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct
     ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc
     acggttacga tgcgcccatc tacaccaacg taacctatcc cattacggtc aatccgccgt
     ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct
     ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt
     ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc
     tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgttggagtg
     acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct
     cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg
     atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact
     acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc
     ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc
     tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg
     ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc
     gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag
     gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg
     tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc
     cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag
     ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc
     taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg
     tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc
     gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca
     ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc
     cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc
     tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact
     ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc
     agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg
     gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc
     cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg
     ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc
     actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg
     ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc
     aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc
     acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg
     cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta
     ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata
     aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca
     ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg
     cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg
     cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc
     ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg
     cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag
     agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg
     cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga
     gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg
     gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc
     gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga
     ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg agcgccggtc
     gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg ccatgtctgc
     ccgtatttcg cgtaaggaaa tccattatgt actatttcgc ctgatgcggt attttctcct
     tacgcatctg tgcggtattt cacaccgcat agggtaataa ctgatataat taaattgaag
     ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct
     ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct
     tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag
     agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac
     ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt
     gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag
     tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc
     taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca
     ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt
     actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat
     tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca
     agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca
     gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca
     tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg
     tagctttcga catgatttat cttcgttttt tgttctgtgc agttgggtta agaatactgg
     gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
     cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaagaaaccg
     aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat tgaaaagcta cttgttaccc
     atcattgaat tttgaacatc cgaacctggg agttttccct gaaacagata gtatatttga
     acctgtataa taatatatag tctagcgctt tacggaagac aatgtatgta tttcggttcc
     tggagaaact attgcatcta ttgcataggt aatcttgcac gtcgcatccc cggttcattt
     tctgcgtttc catcttgcac ttcaatagca tatctttgtt aacgaagcat ctgtgcttca
     ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc
     ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc
     tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca
     tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt
     gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata
     aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt
     tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt
     gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt
     ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt
     cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac
     ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag
     gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt
     ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt ttggtttttt
     gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc ctatactttc
     tagctagaga ataggaactt cggaatagga acttcaaagc gtttccgaaa acgagcgctt
     ccgaaaatgc aacgcgagct gcgcacatac agctcactgt tcacgtcgca cctatatctg
     cgtgttgcct gtatatatat atacatgaga agaacggcat agtgcgtgtt tatgcttaaa
     tgcgttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     cgcgcgttgg ccgattcatt cccg
//