back Return to this vector's summary.
ID   YEP354     preliminary; circular DNA; SYN; 7942 BP.
XX
AC   ATCC37726;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector YEp354 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   YEp352E from YEp352 & linker
RC   YEp363A from pNM480 & YEp351
RC   YEp353A from pNM480 & YEp352
RC   YEp353 from YEp353A & YEp352E
RC   YEp354A from pNM481 & YEp352
RC   YEp354 from YEp354A & YEp352E
RC   YEp355A from pNM482 & YEp352
RC   YEp355 from YEp355A & YEp352E
RC   YEp356, YEp356R from YEp353 & pUC18
RC   YEp357, YEp357R from YEp354 & pUC18
RC   YEp358, YEp358R from YEp355 & pUC18
RC   YEp363 from YEp363A & YEp353
RC   YEp364 from YEp363A & YEp354
RC   YEp365 from YEp363A & YEp355
RC   YEp366 from YEp363A & YEp356
RC   YEp367 from YEp363A & YEp357
RC   YEp368 from YEp363A & YEp358
RC   YEp366R from YEp363A & YEp356R
RC   YEp367R from YEp363A & YEp357R
RC   YEp368R from YEp363A & YEp358R
RC   YIp353 from YEp353 & YIp352
RC   YIp354 from YEp354 & YIp352
RC   YIp355 from YEp355 & YIp352
RC   YIp356 from YEp356 & YIp352
RC   YIp357 from YEp357 & YIp352
RC   YIp358 from YEp358 & YIp352
RC   YIp356R from YEp356R & YIp352
RC   YIp357R from YEp357R & YIp352
RC   YIp358R from YEp358R & YIp352
RC   YIp363 from YEp363 & YIp351
RC   YIp364 from YEp364 & YIp351
RC   YIp365 from YEp365 & YIp351
RC   YIp366 from YEp366 & YIp351
RC   YIp367 from YEp367 & YIp351
RC   YIp368 from YEp368 & YIp351
RC   YIp366R from YEp366R & YIp351
RC   YIp367R from YEp367R & YIp351
RC   YIp368R from YEp368R & YIp351
RA   Myers A.M., Tzagoloff A., Kinney D.M., Lusty C.J.;
RT   "Yeast shuttle and integrative vectors with multiple cloning sites
RT   suitable for construction of lacZ fusions";
RL   Gene 45:299-310(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   The cleavage position in the reading frame for cloning sites is (where
CC   3 = between triplets): EcoRI-1; SmaI-2; BamHI-2; SalI-2; PstI-3;
CC   HindIII-1.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--8.0; HindIII--8.0; SmaI--8.0; BamHI--8.0; PstI--8.0. (ATCC
CC   staff)
CC   The sequence and reading frame of the multiple cloning sequence is:
CC   5' GAA TTC CCG GGG ATC CGT CGA CCT GCA GCC AAG CTT GCT CCC 3'.
CC   The sequence begins with the first nucleotide of the MCS & ends with
CC   the CCC for amino acid 8 of beta-galactosidase.
CC   One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37725 to
CC   ATCC 37727) with URA3 selection in Saccharomyces cerevisiae, a
CC   beta-galactosidase reporter gene and multiple cloning sites differing
CC   in reading frame.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YEp354)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(E.coli MC1061)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (YEp352)(pNM481)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp352 remove PvuII-PvuII 322bp
FT                   \ 5021..5181..162, lacZ'/lacI genes
FT                   S1 nuclease [removes ATG 5' to PvuII, 6bp]
FT                   2. EcoRI linker 12bp cccggattcggg
FT                   -> YEp352E 4865bp
FT                   1. pNM481 EcoRI-DraI 3150bp
FT                   2. YEp352 remove EcoRI-NarI 216bp 16..232, 5181bp
FT                   -> YEp354A 8115bp
FT                   1. YEp354A EcoRI-NcoI 3900bp 16..,
FT                   \ MCS/lacZ/URA3 YEp352 16..232-pNM481-233..958
FT                   2. YEp352E NcoI-EcoRI 4063bp 958..5021
FT                   -> YEp354 7963bp"
FT   -               1..38
FT                   /note="YEp353 1..38 38bp"
FT   -               39..7942
FT                   /note="YEp353 41..7944 7904bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
XX
SQ   Sequence 7942 BP; 1980 A; 1955 C; 1934 G; 2073 T; 0 other;
     gaattcccgg ggatccgtcg acctgcagcc aagcttgctc ccgccgtcgt tttacaacgt
     cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
     ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac
     ggttacgatg cgcccatcta caccaacgta acctatccca ttacggtcaa tccgccgttt
     gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg
     ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg
     tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg
     agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ttggagtgac
     ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg
     ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat
     gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac
     ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct
     ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg
     aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt
     gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc
     gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc
     gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
     caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg
     aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc
     aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta
     ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg
     atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc
     tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc
     acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg
     gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg
     atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg
     caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag
     tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc
     gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg
     catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg
     caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac
     tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct
     ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa
     ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac
     atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg
     tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat
     aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa
     aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt
     ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg
     ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg
     gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg
     aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg
     agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag
     cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc
     tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc
     gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc
     gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc
     catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt
     ggtggcgacg actcctggag cccgtcagta tcggcggaat tccagctgag cgccggtcgc
     taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc
     gtatttcgcg taaggaaatc cattatgtac tatttcgcct gatgcggtat tttctcctta
     cgcatctgtg cggtatttca caccgcatag ggtaataact gatataatta aattgaagct
     ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta gttttgctgg
     ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta
     gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag
     accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc
     acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga
     gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta
     tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta
     ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc
     acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac
     tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg
     tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag
     atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt
     aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg
     atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta
     gctttcgaca tgatttatct tcgttttttg ttctgtgcag ttgggttaag aatactgggc
     aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct
     tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa agaaaccgaa
     atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg aaaagctact tgttacccat
     cattgaattt tgaacatccg aacctgggag ttttccctga aacagatagt atatttgaac
     ctgtataata atatatagtc tagcgcttta cggaagacaa tgtatgtatt tcggttcctg
     gagaaactat tgcatctatt gcataggtaa tcttgcacgt cgcatccccg gttcattttc
     tgcgtttcca tcttgcactt caatagcata tctttgttaa cgaagcatct gtgcttcatt
     ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
     catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
     ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta
     tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc
     ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc
     actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa
     aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt
     tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga
     acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct
     attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca
     ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat
     aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt
     tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg
     aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga
     aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta
     gctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc
     gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg
     tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg
     cgttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     cgcgttggcc gattcattcc cg
//