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ID   YEP355     preliminary; circular DNA; SYN; 7943 BP.
XX
AC   ATCC37727;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector YEp355 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   YEp352E from YEp352 & linker
RC   YEp363A from pNM480 & YEp351
RC   YEp353A from pNM480 & YEp352
RC   YEp353 from YEp353A & YEp352E
RC   YEp354A from pNM481 & YEp352
RC   YEp354 from YEp354A & YEp352E
RC   YEp355A from pNM482 & YEp352
RC   YEp355 from YEp355A & YEp352E
RC   YEp356, YEp356R from YEp353 & pUC18
RC   YEp357, YEp357R from YEp354 & pUC18
RC   YEp358, YEp358R from YEp355 & pUC18
RC   YEp363 from YEp363A & YEp353
RC   YEp364 from YEp363A & YEp354
RC   YEp365 from YEp363A & YEp355
RC   YEp366 from YEp363A & YEp356
RC   YEp367 from YEp363A & YEp357
RC   YEp368 from YEp363A & YEp358
RC   YEp366R from YEp363A & YEp356R
RC   YEp367R from YEp363A & YEp357R
RC   YEp368R from YEp363A & YEp358R
RC   YIp353 from YEp353 & YIp352
RC   YIp354 from YEp354 & YIp352
RC   YIp355 from YEp355 & YIp352
RC   YIp356 from YEp356 & YIp352
RC   YIp357 from YEp357 & YIp352
RC   YIp358 from YEp358 & YIp352
RC   YIp356R from YEp356R & YIp352
RC   YIp357R from YEp357R & YIp352
RC   YIp358R from YEp358R & YIp352
RC   YIp363 from YEp363 & YIp351
RC   YIp364 from YEp364 & YIp351
RC   YIp365 from YEp365 & YIp351
RC   YIp366 from YEp366 & YIp351
RC   YIp367 from YEp367 & YIp351
RC   YIp368 from YEp368 & YIp351
RC   YIp366R from YEp366R & YIp351
RC   YIp367R from YEp367R & YIp351
RC   YIp368R from YEp368R & YIp351
RA   Myers A.M., Tzagoloff A., Kinney D.M., Lusty C.J.;
RT   "Yeast shuttle and integrative vectors with multiple cloning sites
RT   suitable for construction of lacZ fusions";
RL   Gene 45:299-310(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   The sequence and reading frame of the multiple cloning sequence is:
CC   5' G AAT TCC CGG GGA TCC GTC GAC CTG CAG CCA AGC TTC GAT CCC 3'.
CC   The sequence begins with the first nucleotide of the MCS & ends with
CC   the CCC for amino acid 8 of beta-galactosidase.
CC   One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37725 to
CC   ATCC 37727) with URA3 selection in Saccharomyces cerevisiae, a
CC   beta-galactosidase reporter gene and multiple cloning sites differing
CC   in reading frame.
CC   The cleavage position in the reading frame for cloning sites is (where
CC   3 = between triplets): EcoRI-3; SmaI-1; BamHI-1; SalI-1; PstI-2;
CC   HindIII-3.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--8.0; HindIII--8.0; SmaI--8.0; BamHI--8.0; PstI--8.0. (ATCC
CC   staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YEp355)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(E.coli MC1061)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (YEp352)(pNM482)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp352 remove PvuII-PvuII 322bp,
FT                   \ 5021..5181..162, lacZ'/lacI genes
FT                   S1 nuclease [removes ATG 5' to PvuII, 6bp]
FT                   2. EcoRI linker 12bp cccggattcggg
FT                   -> YEp352E 4865bp
FT                   1. pNM482 EcoRI-DraI 3150bp
FT                   2. YEp352 remove EcoRI-NarI 216bp 16..232, 5181bp
FT                   -> YEp355A 8115bp
FT                   1. YEp355A EcoRI-NcoI 3900bp 16..,
FT                   \ MCS/lacZ/URA3 YEp352 16..232-pNM481-233..958
FT                   2. YEp352E EcoRI-NcoI 4063bp 958..5021
FT                   -> YEp355 7963bp"
FT   -               1..36
FT                   /note="YEp353 1..36 36bp"
FT   -               37..7943
FT                   /note="YEp353 38..7944 7907bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
XX
SQ   Sequence 7943 BP; 1981 A; 1955 C; 1934 G; 2073 T; 0 other;
     gaattcccgg ggatccgtcg acctgcagcc aagcttcgat cccgccgtcg ttttacaacg
     tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg
     gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca
     cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt
     tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg
     gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg
     gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct
     gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gttggagtga
     cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc
     gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga
     tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta
     cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc
     tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct
     gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt
     tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg
     cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg
     cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt
     gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc
     gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc
     caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct
     accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt
     gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg
     ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac
     cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc
     ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct
     gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg
     gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca
     gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg
     cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc
     gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg
     gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca
     ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc
     tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca
     actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca
     catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc
     gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa
     taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa
     aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat
     tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc
     gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc
     ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg
     gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc
     gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga
     gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc
     ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag
     cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg
     cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg
     ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat
     tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttccagctga gcgccggtcg
     ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc
     cgtatttcgc gtaaggaaat ccattatgta ctatttcgcc tgatgcggta ttttctcctt
     acgcatctgt gcggtatttc acaccgcata gggtaataac tgatataatt aaattgaagc
     tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg
     gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt
     agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga
     gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc
     cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg
     agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt
     atattctcca gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct
     aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac
     cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta
     ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt
     gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa
     gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag
     taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat
     gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt
     agctttcgac atgatttatc ttcgtttttt gttctgtgca gttgggttaa gaatactggg
     caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
     ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aagaaaccga
     aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt gaaaagctac ttgttaccca
     tcattgaatt ttgaacatcc gaacctggga gttttccctg aaacagatag tatatttgaa
     cctgtataat aatatatagt ctagcgcttt acggaagaca atgtatgtat ttcggttcct
     ggagaaacta ttgcatctat tgcataggta atcttgcacg tcgcatcccc ggttcatttt
     ctgcgtttcc atcttgcact tcaatagcat atctttgtta acgaagcatc tgtgcttcat
     tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
     atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct
     tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
     gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct
     atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat
     cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg
     cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa
     aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt
     ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg
     aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc
     tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc
     actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca
     taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg
     ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg
     gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg
     aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct
     agctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc
     cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc
     gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat
     gcgttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     gcgcgttggc cgattcattc ccg
//