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ID   YIP30      preliminary; circular DNA; SYN; 5527 BP.
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AC   ATCC37109;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YIp30 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   from pBR322 & yeast 2 micron
RA   Botstein D., Falco S.C., Stewart S.E., Brennan M., Scherer S.,
RA   Stinchcomb D.T., Struhl K., Davis R.W.;
RT   "Sterile host yeasts (SHY): a eukaryotic system of biological
RT   containment for recombinant DNA experiments";
RL   Gene 8:17-24(1979).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   This was constructed by cloning the 1.1 kb HindIII fragment containing
CC   URA3 into the HindIII site of pBR322.  The 5' - 3' orientation of URA3
CC   is counterclockwise, with the SmaI site in 3' URA3 clone to the EcoRI
CC   site of pBR322. [2]
CC   HV2 vector when used with S. cerevisiae SHY series hosts (ATCC 44769
CC   to ATCC 44772). (ATCC staff)
CC   A general purpose integrating Saccharomyces cerevisiae/E.coli
CC   shuttle vector. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YIp30)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli MM294)
CC   HO (E.coli C600)(S.cerevisiae SHY series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(YEp24)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII 4361bp 30..30
FT                   2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   -> YIp30 5527bp [SmaI in URA3 3' LTR close to EcoRI]"
FT   -               1..29
FT                   /note="pBR322 1..29 29bp
FT                   HindIII = A^AGCTT"
FT   -               30..1195
FT                   /note="YEp24 2277..3442 1166bp complement
FT                   HindIII = A^AGCTT"
FT   -               1196..5527
FT                   /note="pBR322 30..4361 4332bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   misc_binding    0..0
FT                   /note="SIT unique SmaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
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SQ   Sequence 5527 BP; 1311 A; 1480 C; 1320 G; 1416 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttttctt tccaattttt tttttttcgt
     cattataaaa atcattacga ccgagattcc cgggtaataa ctgatataat taaattgaag
     ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct
     ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct
     tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag
     agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac
     ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt
     gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag
     tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc
     taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca
     ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt
     actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat
     tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca
     agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca
     gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca
     tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg
     tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta
     agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa
     gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc
     aaagaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat tgaaaagctt
     taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct
     aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
     gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt
     cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc
     ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc
     actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc
     atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc
     tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga
     atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca
     cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
     cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
     tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
     gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
     ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
     ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
     ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
     cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc
     ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct
     ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac
     aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg
     cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
     gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     tcaagaa
//