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ID   YIP351     preliminary; circular DNA; SYN; 4406 BP.
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AC   L14756; ATCC37674;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YIp351 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4406
RC   YEp351 from pUC18 & pC4B, yeast 2 micron/LEU2 gene
RC   YEp352 from pUC18 & yeast 2 micron & yeast URA3 gene
RC   YIp351 from YEp351
RC   YIp352 from YEp352
RA   Hill J.E., Myers A.M., Koerner T.J., Tzagoloff A.;
RT   "Yeast/E. coli shuttle vectors with multiple unique restriction
RT   sites";
RL   Yeast 2:163-167(1986).
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CC   Constructed from YEp351 (ATCC 37672) by deleting all 2 micron circle
CC   sequences by digestion with HpaI and AatII (nt 2259-3492).
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--4.4; XbaI--4.4; PstI--4.4; HindIII--4.4. (ATCC staff)
CC   This is an E.coli/Saccharomyces integrative shuttle vector with
CC   multiple cloning sites.
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 295272
CC   NM (YIp351)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(S.cerevisiae)(E.coli)(Saccharomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(2micron)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp351 remove HpaI-AatII 1238bp 2259..3497,
FT                   \ yeast 2 micron/4406bp
FT                   -> YIp351 4406bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-
FT                   PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique SacI-SmaI-BamHI-XbaI-SalI-PstI-
FT                   SphI-HindIII-Narl-BstXI-AflII-ClaI-BstEII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
FT   misc_feature    1..285
FT                   /note="from Escherichia coli"
FT   misc_feature    286..2258
FT                   /note="from Saccharomyces cerevisiae"
FT   misc_feature    2259..4406
FT                   /note="from Escherichia coli"
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SQ   Sequence 4406 BP; 1171 A; 1014 C; 986 G; 1235 T; 0 other;
     atgaccatga ttacgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg
     catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     acccaactta atcgccttgc agcacatccc cccttcgcca gctggcgtaa tagcgaagag
     gcccgcaccg atcgcccttc ccaacagttg cgtagcctga atggcgaatg gcgcctgatg
     cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagatcc ggtacgtcgt
     taaggccgtt tctgacagag taaaattctt gagggaactt tcaccattat ggaaaatggt
     tcaagaaggt attgacttaa actccatcaa atggtcaggt catttgagtg ttttttattt
     gttgtatttt ttttttttta gagaaaatcc tccaatatca aattagggat tcgtagtttc
     atgattttct gttataccta actttttgtg tggtgccctc ctccttgtca atattaatgt
     taaagtgcaa ttctctttcc ttatcacgtt gagccattag tatcaatttg cttacctgta
     ttcctttagc tatcctcctt tttctccttc ttgataaatg tatgtagatt gcgtttgtct
     accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat
     aaagtttatg tacaaatatc ataaaaaaga gaatcttttt aagcaaggat tttcttaact
     tcttcggcga cagcatcacc gacttcggtg gtactgttgg aaccacctaa atcaccagtt
     ctgatacctg catccaaaac ctttttaact gcatcttcaa tggccttacc ttcttcaggc
     aagttcaatg acaatttcaa catcattgca gcagacaaga tagtggcgat agggtcaacc
     ttattcttgg caaatccggg agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg
     ttcttgtctg gcaaagaggc caaggacgca gatggcaaca aacccaagga acctggataa
     cgtaaggctt catcggagat gatatcacca aacatgttgc tggtgattat aataccattt
     aggtgggttg ggttcttaac taggatcatg gcggcagaat caatcaattg atgttgaacc
     ttcaatgtag ggaattcgtt cttgatggtt tcctccacag tttttctcca taatcttgaa
     gaggccaaaa cattagcttt atccaaggtc caaataggca atggtggctc atgttgtagg
     gccatgaaag cggccattct tgtgattctt tgcacttctg gaacggtgta ttgttcacta
     tcccaagcga caccatcacc atcgtcttcc tttctcttac caaagtaaat acctcccact
     aattctctga caacaacgaa gtcagtacct ttagcaaatt gtggcttgat tggagataag
     tctaaaagag agtcggatgc aaagttacat ggtcttaagt tggcgtacaa ttgaagttct
     ttacggattt ttagtaaacc ttgttcaggt ctaacactac cggtacccca ttttggacca
     cccacagcac ctaacaaaac ggcatcaacc ttcttggagg cttccagtgc ctcatctgga
     agtgggacac ctgtagcatc gatagcagca ccaccaatta aatgattttc gaaatcgaac
     ttgacattgg aacgaacatc agaaatagct ttaagaacct taatggcttc ggctgtgatt
     tcttgaccaa cgtggtcacc tggcaaaacg acgatcttct taggaacaaa cattagaatg
     gtatatcctt gaaatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa
     gtaagacgat tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa
     cttcaagtat tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc
     cggttccggc ctctcacctt tcctttttct cccaattttt cagttgaaaa aggtatatgc
     gtcaggcgac ctctgaaatt aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat
     agcgcccctg tgtgttctcg ttatgttgag gaaaaaaata atggttgcta agagattcga
     actcttgcat cttacgatac ctgagtattc ccacagttca ggtggcactt ttcggggaaa
     tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
     gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     acgcaaaccg cctctccccg cgcgttggcc gattcattaa tccagctggc acgacaggtt
     tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttacc tcactcatta
     ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     ataacaattt cacacaggaa acagct
//