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ID   YIP352     preliminary; circular DNA; SYN; 4303 BP.
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AC   L14759; ATCC37675;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YIp352 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4303
RC   YEp351 from pUC18 & pC4B, yeast 2 micron/LEU2 gene
RC   YEp352 from pUC18 & yeast 2 micron & yeast URA3 gene
RC   YIp351 from YEp351
RC   YIp352 from YEp352
RA   Hill J.E., Myers A.M., Koerner T.J., Tzagoloff A.;
RT   "Yeast/E. coli shuttle vectors with multiple unique restriction
RT   sites";
RL   Yeast 2:163-167(1986).
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CC   Constructed from YEp352 (ATCC 37673) by cleavage at the SspI and HpaI
CC   sites in the 2 micron sequence.  Some 2 micron sequence remains, but
CC   replicative function has been lost.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--4.3; XbaI--4.3; PstI--4.3; HindIII--4.3. (ATCC staff)
CC   This is an E.coli/Saccharomyces integrative shuttle vector with
CC   multiple cloning sites. (personal communication)
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 295273
CC   NM (YIp352)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(S.cerevisiae)(E.coli)(Saccharomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(2micron)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp352 remove HpaI-SspI 878bp 1614..2492,
FT                   \ yeast 2 micron/4303bp
FT                   -> YIp352 4303bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-
FT                   PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-
FT                   SalI-PstI-SphI-HindIII-Narl-
FT                   StuI-ApaI-AsuII-NcoI-NdeI-AatII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   misc_feature    1..285
FT                   /note="from Escherichia coli"
FT   misc_feature    286..1610
FT                   /note="from Saccharomyces cerevisiae"
FT   misc_feature    1611..4303
FT                   /note="from Escherichia coli"
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SQ   Sequence 4303 BP; 1103 A; 1050 C; 961 G; 1189 T; 0 other;
     atgaccatga ttacgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg
     catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     acccaactta atcgccttgc agcacatccc cccttcgcca gctggcgtaa tagcgaagag
     gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagggta ataactgata
     taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt
     tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg
     ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat
     gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc
     cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc
     acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc
     aacagtaccc ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa
     catcaaaagg cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac
     aatacctggg cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta
     tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc
     gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat
     ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc
     ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt
     ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc
     acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcggttttt gttctgtgca
     gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata
     ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa
     aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa
     gctcttgtta cccatcattg aattttgaac atccgaacct gggagttttc cctgaaacag
     atagtatatt tgaacctgta taataatata tagtctagcg ctttacggaa gacaatgtat
     gtatttcggt tcctggagaa actattgcat ctattgcata ggtaatcttg cacgtcgcat
     ccccggttca ttttctgcgt ttccatcttg cacttcaata gcatatcttt gttattttag
     tagctcgtta cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt
     ggttttcaaa agcgctctga agttcctata ctttctagct agagaatagg aacttcggaa
     taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca
     catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca
     tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt tatggtgcac tctcagtaca
     atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatcc
     agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     agttacctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     tgtggaattg tgagcggata acaatttcac acaggaaaca gct
//