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ID   YIP353     preliminary; circular DNA; SYN; 7065 BP.
XX
AC   ATCC37743;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector YIp353 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   YEp352E from YEp352 & linker
RC   YEp363A from pNM480 & YEp351
RC   YEp353A from pNM480 & YEp352
RC   YEp353 from YEp353A & YEp352E
RC   YEp354A from pNM481 & YEp352
RC   YEp354 from YEp354A & YEp352E
RC   YEp355A from pNM482 & YEp352
RC   YEp355 from YEp355A & YEp352E
RC   YEp356, YEp356R from YEp353 & pUC18
RC   YEp357, YEp357R from YEp354 & pUC18
RC   YEp358, YEp358R from YEp355 & pUC18
RC   YEp363 from YEp363A & YEp353
RC   YEp364 from YEp363A & YEp354
RC   YEp365 from YEp363A & YEp355
RC   YEp366 from YEp363A & YEp356
RC   YEp367 from YEp363A & YEp357
RC   YEp368 from YEp363A & YEp358
RC   YEp366R from YEp363A & YEp356R
RC   YEp367R from YEp363A & YEp357R
RC   YEp368R from YEp363A & YEp358R
RC   YIp353 from YEp353 & YIp352
RC   YIp354 from YEp354 & YIp352
RC   YIp355 from YEp355 & YIp352
RC   YIp356 from YEp356 & YIp352
RC   YIp357 from YEp357 & YIp352
RC   YIp358 from YEp358 & YIp352
RC   YIp356R from YEp356R & YIp352
RC   YIp357R from YEp357R & YIp352
RC   YIp358R from YEp358R & YIp352
RC   YIp363 from YEp363 & YIp351
RC   YIp364 from YEp364 & YIp351
RC   YIp365 from YEp365 & YIp351
RC   YIp366 from YEp366 & YIp351
RC   YIp367 from YEp367 & YIp351
RC   YIp368 from YEp368 & YIp351
RC   YIp366R from YEp366R & YIp351
RC   YIp367R from YEp367R & YIp351
RC   YIp368R from YEp368R & YIp351
RA   Myers A.M., Tzagoloff A., Kinney D.M., Lusty C.J.;
RT   "Yeast shuttle and integrative vectors with multiple cloning sites
RT   suitable for construction of lacZ fusions";
RL   Gene 45:299-310(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   The sequence and reading frame of the multiple cloning sequence is:
CC   5' GA ATT CCC GGG GAT CCG TCG ACC TGC AGC CAA GCT TGC GAT CCC 3'.
CC   The sequence begins with the first nucleotide of MCS and ends with the
CC   CCC for amino acid 8 of beta-galactosidase.
CC   One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37743 to
CC   ATCC 37745) with URA3 selection in Saccharomyces cerevisiae, a
CC   beta-galactosidase reporter gene and multiple cloning sites differing
CC   in reading frame.
CC   The cleavage position in the reading frame for cloning sites is (where
CC   3 = between triplets): EcoRI-2; SmaI-3; BamHI-3; SalI-3; PstI-1;
CC   HindIII-2.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--7.1; BamHI--7.1; SalI--7.1; PstI--7.1; HindIII--7.1. (ATCC
CC   staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YIp353)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(E.coli MC1061)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (YEp353)(YIp352)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp353 AatII-NcoI 5867bp 5961..7944..3884
FT                   2. YIp352 NcoI-AatII 1198bp 958..2156, URA3/pUC18
FT                   -> YIp353 7065bp"
FT   -               1..5867
FT                   /note="YEp353 5961..7944..3883 5867bp
FT                   NcoI = C^CATGG"
FT   -               5868..7065
FT                   /note="YIp352 958..2155 1198bp
FT                   AatII = GACGT^C"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   reporter gene"
XX
SQ   Sequence 7065 BP; 1704 A; 1805 C; 1767 G; 1789 T; 0 other;
     caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     cccggaattc ccggggatcc gtcgacctgc agccaagctt gcgatcccgc cgtcgtttta
     caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
     agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag
     atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg
     ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa
     agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat
     ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt
     gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgttgg
     agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac
     gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt
     aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt
     gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc
     gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta
     cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg
     gtggttgaac tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt
     ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt
     cgaggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg
     atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat
     tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat
     gaagccaata ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc
     tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg
     agtgtgatca tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg
     tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc
     gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc
     ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc
     ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa
     tactggcagg cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg
     gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat
     tttggcgata cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc
     acgccgcatc cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta
     tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc
     ctgcactgga tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat
     gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc
     gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc
     gggcacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc
     gccgcgtccc acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg
     ggtaataagc gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc
     gataaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac
     gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag
     gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct
     gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc
     agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa
     gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta
     gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact
     gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc
     ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg
     cgcggcgact tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc
     catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg
     gggattggtg gcgacgactc ctggagcccg tcagtatcgg cggaattcca gctgagcgcc
     ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg caggccatgt
     ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt tcgcctgatg cggtattttc
     tccttacgca tctgtgcggt atttcacacc gcatagggta ataactgata taattaaatt
     gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt
     tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct
     accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct
     gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct
     aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct
     ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc
     ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg
     cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg
     cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca
     gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa
     aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca
     gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac
     tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg
     tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta
     tatgtagctt tcgacatgat ttatcttcgt ttcggttttt gttctgtgca gttgggttaa
     gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag
     tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca
     aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa gctcttgtta
     cccatcattg aattttgaac atccgaacct gggagttttc cctgaaacag atagtatatt
     tgaacctgta taataatata tagtctagcg ctttacggaa gacaatgtat gtatttcggt
     tcctggagaa actattgcat ctattgcata ggtaatcttg cacgtcgcat ccccggttca
     ttttctgcgt ttccatcttg cacttcaata gcatatcttt gttattttag tagctcgtta
     cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa
     agcgctctga agttcctata ctttctagct agagaatagg aacttcggaa taggaacttc
     aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc
     actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac
     ggcatagtgc gtgtttatgc ttaaatgcgt tatggtgcac tctcagtaca atctgctctg
     atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     tatttttata ggttaatgtc atgataataa tggtttctta gacgt
//