back Return to this vector's summary.
ID   YIP354     preliminary; circular DNA; SYN; 7063 BP.
XX
AC   ATCC37744;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector YIp354 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   YEp352E from YEp352 & linker
RC   YEp363A from pNM480 & YEp351
RC   YEp353A from pNM480 & YEp352
RC   YEp353 from YEp353A & YEp352E
RC   YEp354A from pNM481 & YEp352
RC   YEp354 from YEp354A & YEp352E
RC   YEp355A from pNM482 & YEp352
RC   YEp355 from YEp355A & YEp352E
RC   YEp356, YEp356R from YEp353 & pUC18
RC   YEp357, YEp357R from YEp354 & pUC18
RC   YEp358, YEp358R from YEp355 & pUC18
RC   YEp363 from YEp363A & YEp353
RC   YEp364 from YEp363A & YEp354
RC   YEp365 from YEp363A & YEp355
RC   YEp366 from YEp363A & YEp356
RC   YEp367 from YEp363A & YEp357
RC   YEp368 from YEp363A & YEp358
RC   YEp366R from YEp363A & YEp356R
RC   YEp367R from YEp363A & YEp357R
RC   YEp368R from YEp363A & YEp358R
RC   YIp353 from YEp353 & YIp352
RC   YIp354 from YEp354 & YIp352
RC   YIp355 from YEp355 & YIp352
RC   YIp356 from YEp356 & YIp352
RC   YIp357 from YEp357 & YIp352
RC   YIp358 from YEp358 & YIp352
RC   YIp356R from YEp356R & YIp352
RC   YIp357R from YEp357R & YIp352
RC   YIp358R from YEp358R & YIp352
RC   YIp363 from YEp363 & YIp351
RC   YIp364 from YEp364 & YIp351
RC   YIp365 from YEp365 & YIp351
RC   YIp366 from YEp366 & YIp351
RC   YIp367 from YEp367 & YIp351
RC   YIp368 from YEp368 & YIp351
RC   YIp366R from YEp366R & YIp351
RC   YIp367R from YEp367R & YIp351
RC   YIp368R from YEp368R & YIp351
RA   Myers A.M., Tzagoloff A., Kinney D.M., Lusty C.J.;
RT   "Yeast shuttle and integrative vectors with multiple cloning sites
RT   suitable for construction of lacZ fusions";
RL   Gene 45:299-310(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   The sequence and reading frame of the multiple cloning sequence is:
CC   5' GAA TTC CCG GGG ATC CGT CGA CCT GCA GCC AAG CTT GCT CCC 3'.
CC   The sequence begins with the first nucleotide of MCS and ends with the
CC   CCC for amino acid 8 of beta-galactosidase.
CC   One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37743 to
CC   ATCC 37745) with URA3 selection in Saccharomyces cerevisiae, a
CC   beta-galactosidase reporter gene and multiple cloning sites differing
CC   in reading frame.
CC   The cleavage position in the reading frame for cloning sites is (where
CC   3 = between triplets): EcoRI-1; SmaI-2; BamHI-2; SalI-2; PstI-3;
CC   HindIII-1.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--7.1; BamHI--7.1; SalI--7.1; PstI--7.1; HindIII--7.1. (ATC
CC   staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YIp354)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(E.coli MC1061)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (YEp354)(YIp352)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp354 AatII-NcoI 5867bp 5961..7944..3884
FT                   2. YIp352 NcoI-AatII 1198bp 958..2156, URA3/pUC18
FT                   -> YIp354 7065bp"
FT   -               1..2022
FT                   /note="YEp353 5961..7944..38 2022bp"
FT   -               2023..5865
FT                   /note="YEp353 41..3883 3843bp
FT                   NcoI = C^CATGG"
FT   -               5866..7063
FT                   /note="YIp352 958..2155 1198bp
FT                   AatII = GACGT^C"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   reporter gene"
XX
SQ   Sequence 7063 BP; 1703 A; 1805 C; 1766 G; 1789 T; 0 other;
     caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     cccggaattc ccggggatcc gtcgacctgc agccaagctt gctcccgccg tcgttttaca
     acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
     ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat
     gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc
     gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag
     ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct
     gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga
     cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgttggag
     tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt
     ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca ctcgctttaa
     tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg agttgcgtga
     ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc
     gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacactacg
     tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt
     ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg atgtcggttt
     ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg
     aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat
     ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct gttcgcatta
     tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga
     agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg
     gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag
     tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg acgcgctgta
     tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg gcggagccga
     caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt
     cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag agacgcgccc
     gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt tcgctaaata
     ctggcaggcg tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga
     tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt
     tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac
     gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt tccgtttatc
     cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct
     gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt
     cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg
     gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg
     gcacatcagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc
     cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg
     taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga
     taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga
     cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc
     ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga
     tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag
     ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt
     ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc
     agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc
     cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc
     gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg
     cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca
     tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg
     gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattccagc tgagcgccgg
     tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca ggccatgtct
     gcccgtattt cgcgtaagga aatccattat gtactatttc gcctgatgcg gtattttctc
     cttacgcatc tgtgcggtat ttcacaccgc atagggtaat aactgatata attaaattga
     agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg
     ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac
     cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt
     agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa
     acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct
     ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt
     agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc
     tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc
     caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga
     gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa
     attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt
     caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc
     cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg
     catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata
     tgtagctttc gacatgattt atcttcgttt cggtttttgt tctgtgcagt tgggttaaga
     atactgggca atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc
     tgtgctcctt ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaaa
     gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagc tcttgttacc
     catcattgaa ttttgaacat ccgaacctgg gagttttccc tgaaacagat agtatatttg
     aacctgtata ataatatata gtctagcgct ttacggaaga caatgtatgt atttcggttc
     ctggagaaac tattgcatct attgcatagg taatcttgca cgtcgcatcc ccggttcatt
     ttctgcgttt ccatcttgca cttcaatagc atatctttgt tattttagta gctcgttaca
     gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
     cgctctgaag ttcctatact ttctagctag agaataggaa cttcggaata ggaacttcaa
     agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac
     tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg
     catagtgcgt gtttatgctt aaatgcgtta tggtgcactc tcagtacaat ctgctctgat
     gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     tttttatagg ttaatgtcat gataataatg gtttcttaga cgt
//