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ID   YIP355     preliminary; circular DNA; SYN; 7064 BP.
XX
AC   ATCC37745;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector YIp355 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   YEp352E from YEp352 & linker
RC   YEp363A from pNM480 & YEp351
RC   YEp353A from pNM480 & YEp352
RC   YEp353 from YEp353A & YEp352E
RC   YEp354A from pNM481 & YEp352
RC   YEp354 from YEp354A & YEp352E
RC   YEp355A from pNM482 & YEp352
RC   YEp355 from YEp355A & YEp352E
RC   YEp356, YEp356R from YEp353 & pUC18
RC   YEp357, YEp357R from YEp354 & pUC18
RC   YEp358, YEp358R from YEp355 & pUC18
RC   YEp363 from YEp363A & YEp353
RC   YEp364 from YEp363A & YEp354
RC   YEp365 from YEp363A & YEp355
RC   YEp366 from YEp363A & YEp356
RC   YEp367 from YEp363A & YEp357
RC   YEp368 from YEp363A & YEp358
RC   YEp366R from YEp363A & YEp356R
RC   YEp367R from YEp363A & YEp357R
RC   YEp368R from YEp363A & YEp358R
RC   YIp353 from YEp353 & YIp352
RC   YIp354 from YEp354 & YIp352
RC   YIp355 from YEp355 & YIp352
RC   YIp356 from YEp356 & YIp352
RC   YIp357 from YEp357 & YIp352
RC   YIp358 from YEp358 & YIp352
RC   YIp356R from YEp356R & YIp352
RC   YIp357R from YEp357R & YIp352
RC   YIp358R from YEp358R & YIp352
RC   YIp363 from YEp363 & YIp351
RC   YIp364 from YEp364 & YIp351
RC   YIp365 from YEp365 & YIp351
RC   YIp366 from YEp366 & YIp351
RC   YIp367 from YEp367 & YIp351
RC   YIp368 from YEp368 & YIp351
RC   YIp366R from YEp366R & YIp351
RC   YIp367R from YEp367R & YIp351
RC   YIp368R from YEp368R & YIp351
RA   Myers A.M., Tzagoloff A., Kinney D.M., Lusty C.J.;
RT   "Yeast shuttle and integrative vectors with multiple cloning sites
RT   suitable for construction of lacZ fusions";
RL   Gene 45:299-310(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37743 to
CC   ATCC 37745) with URA3 selection in Saccharomyces cerevisiae, a
CC   beta-galactosidase reporter gene and multiple cloning sites differing
CC   in reading frame.
CC   The cleavage position in the reading frame for cloning sites is (where
CC   3 = between triplets): EcoRI-3; SmaI-1; BamHI-1; SalI-1; PstI-2;
CC   HindIII-3.
CC   The sequence and reading frame of the multiple cloning sequence is:
CC   5' G AAT TCC CGG GGA TCC GTC GAC CTG CAG CCA AGC TTC GAT CCC 3'.
CC   The sequence begins with the first nucleotide of MCS and ends with the
CC   CCC for amino acid 8 of beta-galactosidase.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--7.1; BamHI--7.1; SalI--7.1; PstI--7.1; HindIII--7.1. (ATCC
CC   staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YIp355)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(E.coli MC1061)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (YEp355)(YIp352)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YEp355 AatII-NcoI 5867bp 5961..7944..3884
FT                   2. YIp352 NcoI-AatII 1198bp 958..2156, URA3/pUC18
FT                   -> YIp355 7065bp"
FT   -               1..2020
FT                   /note="YEp353 5961..7944..36 2020bp"
FT   -               2021..5866
FT                   /note="YEp353 38..3883 3846bp
FT                   NcoI = C^CATGG"
FT   -               5867..7064
FT                   /note="YIp352 958..2155 1198bp
FT                   AatII = GACGT^C"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   reporter gene"
XX
SQ   Sequence 7064 BP; 1704 A; 1805 C; 1766 G; 1789 T; 0 other;
     caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     cccggaattc ccggggatcc gtcgacctgc agccaagctt cgatcccgcc gtcgttttac
     aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
     gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
     tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc
     cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
     gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc
     tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg
     acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgttgga
     gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg
     tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta
     atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg
     actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg
     cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac
     gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg
     tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt
     tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc
     gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga
     tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt
     atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg
     aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct
     ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga
     gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt
     atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg
     acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct
     tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc
     cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat
     actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg
     atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt
     ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca
     cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat
     ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc
     tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg
     tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg
     ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg
     ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg
     ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg
     gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg
     ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg
     acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg
     cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg
     atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca
     gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag
     tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag
     cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg
     ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc
     cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc
     gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc
     atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg
     ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg
     gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggccatgtc
     tgcccgtatt tcgcgtaagg aaatccatta tgtactattt cgcctgatgc ggtattttct
     ccttacgcat ctgtgcggta tttcacaccg catagggtaa taactgatat aattaaattg
     aagctctaat ttgtgagttt agtatacatg catttactta taatacagtt ttttagtttt
     gctggccgca tcttctcaaa tatgcttccc agcctgcttt tctgtaacgt tcaccctcta
     ccttagcatc ccttcccttt gcaaatagtc ctcttccaac aataataatg tcagatcctg
     tagagaccac atcatccacg gttctatact gttgacccaa tgcgtctccc ttgtcatcta
     aacccacacc gggtgtcata atcaaccaat cgtaaccttc atctcttcca cccatgtctc
     tttgagcaat aaagccgata acaaaatctt tgtcgctctt cgcaatgtca acagtaccct
     tagtatattc tccagtagat agggagccct tgcatgacaa ttctgctaac atcaaaaggc
     ctctaggttc ctttgttact tcttctgccg cctgcttcaa accgctaaca atacctgggc
     ccaccacacc gtgtgcattc gtaatgtctg cccattctgc tattctgtat acacccgcag
     agtactgcaa tttgactgta ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa
     aattgtactt ggcggataat gcctttagcg gcttaactgt gccctccatg gaaaaatcag
     tcaagatatc cacatgtgtt tttagtaaac aaattttggg acctaatgct tcaactaact
     ccagtaattc cttggtggta cgaacatcca atgaagcaca caagtttgtt tgcttttcgt
     gcatgatatt aaatagcttg gcagcaacag gactaggatg agtagcagca cgttccttat
     atgtagcttt cgacatgatt tatcttcgtt tcggtttttg ttctgtgcag ttgggttaag
     aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt
     ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa
     agaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaaaag ctcttgttac
     ccatcattga attttgaaca tccgaacctg ggagttttcc ctgaaacaga tagtatattt
     gaacctgtat aataatatat agtctagcgc tttacggaag acaatgtatg tatttcggtt
     cctggagaaa ctattgcatc tattgcatag gtaatcttgc acgtcgcatc cccggttcat
     tttctgcgtt tccatcttgc acttcaatag catatctttg ttattttagt agctcgttac
     agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa
     gcgctctgaa gttcctatac tttctagcta gagaatagga acttcggaat aggaacttca
     aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca
     ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg
     gcatagtgcg tgtttatgct taaatgcgtt atggtgcact ctcagtacaa tctgctctga
     tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     atttttatag gttaatgtca tgataataat ggtttcttag acgt
//