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ID   YIP5       preliminary; circular DNA; SYN; 5541 BP.
XX
AC   L09157; VB0068; IG0130; ATCC37061; K02206; K02207;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YIp5 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5541
RC   YIp5
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [2]
RC   YIp5
RA   ;
RT   ;
RL   Submitted (12-SEP-1991) on magnetic tape by:
RL   New England Biolabs, .
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RN   [3]
RC   pGT2-Sc2605 from yeast HIS3 gene
RC   pBR322-Sc2676 from pBR322 & yeast HIS3 gene
RC   lambda 590-Sc2904 from lambda 590 & pMB1068, yeast URA3 gene
RC   lambda gt-Sc4104 from lambda gt10 & yeast ARS1/TRP1 gene
RC   pT322 from pBR322 & yeast ARS1/TRP1 gene 1.4kb EcoRI-EcoRI
RC   YIp1 from pBR322 & pGT2-Sc2605
RC   YIp5 or pNN36 from pBR322 & lambda 590-Sc2904
RC   YEp2 from pBR322 & SCP1
RC   YEp4 from pBR322 & lambda & SCP1
RC   YEp6 from pBR322-Sc2676 & SCP1
RC   YRp7 or pNN78 from pBR322 & lambda gt-Sc4104
RC   [YEp-3A, YEp-3B from pBR322 & yeast 2 micron]
RC   [YRp12 from YRp series]
RA   Struhl K., Stinchcomb D.T., Scherer S., Davis R.W.;
RT   "High-frequency transformation of yeast: autonomous replication of
RT   hybrid DNA molecules";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:1035-1039(1979).
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   The length of G- or C-tails used in the cloning procedure is
CC   not exactly known. Therefore the sequencea 1428-1437, C(10)
CC   and 2601-2610 G(10) are tentative.
CC   Size could be 5541 bp.
CC   ATCC size is 5500 bp.
CC   This was constructed by cloning the 1.1 kb HindIII fragment containing
CC   URA3 from lambda590-Sc2904 into the AvaI site of pBR322.  The 5' - 3'
CC   orientation of URA3 is clockwise; SmaI & AvaI sites in the 3' of URA3
CC   are close to the PvuII site of pBR322. [7]
CC   A general purpose integrating Saccharomyces cerevisiae/Escherichia
CC   coli shuttle vector. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YIp5)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (NEB)(ATCC)
CC   HO (E.coli K-12 r-m+)(E.coli C600)(E.coli MM294)(E.coli)
CC   HO (S.cerevisiae)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(2micron)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda 590 HindIII
FT                   2. pMB1068 HindIII-HindIII 1166bp, URA3 gene
FT                   \ = YEp24 HindIII-HindIII 1166bp 2273..3439
FT                   -> lambda 590-Sc2904
FT                   1. pBR322 AvaI 4361bp 1426..1426
FT                   fill in
FT                   dC tailing
FT                   2. lambda 590-Sc2904 HindIII-HindIII 1166bp, URA3 gene
FT                   \ = YEp24 HindIII-HindIII 1166bp 2273..3439
FT                   dG tailing
FT                   -> YIp5 5541bp"
FT   misc_feature    4..1428
FT                   /note="from pBR322"
FT   misc_feature    1428..1437
FT                   /note="G-tail (assumed size)"
FT   CDS             1439..2600
FT                   /note="ANT yeast URA3 gene
FT                   (GenBank(50):YSCODCD)"
FT   misc_feature    complement(2601..2610)
FT                   /note="G-tail (assumed size)"
FT   misc_feature    2609..5541
FT                   /note="from pBR322"
FT   misc_feature    0..0
FT                   /note="GC-tail the 1100 bp Ura3 region fragment of
FT                   S. cerevisiae and insert into AvaI site in pBR322"
FT   misc_binding    0..0
FT                   /note="SIT unique ClaI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   CDS             89..1279
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    0..0
FT                   /note="SIT unique NheI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique SphI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoNI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   misc_binding    0..0
FT                   /note="SIT unique PshAI"
FT   misc_binding    0..0
FT                   /note="SIT unique EagI"
FT   misc_binding    0..0
FT                   /note="SIT unique NruI"
FT   misc_binding    0..0
FT                   /note="SIT unique BspMI"
FT   CDS             1661..2464
FT                   /note="ANT yeast URA3 gene (uracil),
FT                   codes for OMP decarboxylase"
FT   misc_binding    0..0
FT                   /note="SIT unique XcmI"
FT   misc_binding    0..0
FT                   /note="SIT unique NcoI"
FT   misc_binding    0..0
FT                   /note="SIT unique BstBI"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI"
FT   misc_binding    0..0
FT                   /note="SIT unique StuI"
FT   misc_binding    0..0
FT                   /note="SIT unique NsiI"
FT   misc_binding    0..0
FT                   /note="SIT unique SmaI"
FT   misc_binding    0..0
FT                   /note="SIT unique MscI"
FT   misc_binding    0..0
FT                   /note="SIT unique BspEI"
FT   misc_binding    0..0
FT                   /note="SIT unique BsaBI"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuII"
FT   misc_binding    0..0
FT                   /note="SIT unique Tth111I"
FT   misc_binding    0..0
FT                   /note="SIT unique BsaAI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(4479..5267)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique Eam1105I"
FT   misc_binding    0..0
FT                   /note="SIT unique AseI"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuI"
FT   misc_binding    0..0
FT                   /note="SIT unique SspI"
FT   misc_binding    0..0
FT                   /note="SIT unique AatII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
XX
SQ   Sequence 5541 BP; 1365 A; 1404 C; 1411 G; 1361 T; 0 other;
     gaattctcat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt
     aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
     cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
     cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
     atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
     ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
     gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc
     gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg
     accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
     agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag
     aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatccc cccccccctt
     tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc tttgaaattt
     ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt
     ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca
     actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag ctacatataa
     ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga
     aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt
     agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga
     tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt
     actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc
     gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc
     aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct
     tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg
     tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga
     catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga
     tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg tctctacagg
     atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga
     gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa
     ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat
     ttaattatat cagttattac ccgggaatct cggtcgtaat gatttttata atgacgaaaa
     aaaaaaaatt ggaaagaaaa gggggggggg gcagcgttgg gtcctggcca cgggtgcgca
     tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
     agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
     cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
     agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct
     gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
     ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
     gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
     ccatgaacag aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc
     ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga
     gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct
     ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     acgaggccct ttcgtcttca a
//