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ID   YRP12      preliminary; circular DNA; SYN; 6798 BP.
XX
AC   IG0131;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector YRp12 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGT2-Sc2605 from yeast HIS3 gene
RC   pBR322-Sc2676 from pBR322 & yeast HIS3 gene
RC   lambda 590-Sc2904 from lambda 590 & pMB1068, yeast URA3 gene
RC   lambda gt-Sc4104 from lambda gt10 & yeast ARS1/TRP1 gene
RC   pT322 from pBR322 & yeast ARS1/TRP1 gene 1.4kb EcoRI-EcoRI
RC   YIp1 from pBR322 & pGT2-Sc2605
RC   YIp5 or pNN36 from pBR322 & lambda 590-Sc2904
RC   YEp2 from pBR322 & SCP1
RC   YEp4 from pBR322 & lambda & SCP1
RC   YEp6 from pBR322-Sc2676 & SCP1
RC   YRp7 or pNN78 from pBR322 & lambda gt-Sc4104
RC   [YEp-3A, YEp-3B from pBR322 & yeast 2 micron]
RC   [YRp12 from YRp series]
RA   Struhl K., Stinchcomb D.T., Scherer S., Davis R.W.;
RT   "High-frequency transformation of yeast: autonomous replication of
RT   hybrid DNA molecules";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:1035-1039(1979).
XX
RN   [2]
RC   pHP34 from pBR322
RC   IAiv3 from pBR322 series
RC   IAiv4 from pBR322 series
RC   IAiv10 from pML series
RC   IAiv17 from pMC1871
RC   IAiv20 from pUC series
RC   IAiv21 from pKO-2
RC   IAv1 from M13 series
RC   IAv2 from M13 series
RC   IAvii3 from pHC79
RC   IBii3 from pWT series
RC   IBii8 from pTrS series
RC   IBiii1 from pUC series
RC   IBiii5 from pUR series
RC   IBiv8 from pMBL604
RC   IBiv13 from pSP series
RC   IBiv15 from pTZ series
RC   ICi1 from pKO series
RC   ICi2 from promoter-analysis vectors
RC   ICi7 from pMC series
RC   IVAai1 from pUB110
RC   VIAii1 from YRp series
RC   VIAii4 from YRp series
RC   VIAiii1 from YIp series
RC   VIIIBbi9 from pSV series
RA   Pouwels P.H., Enger-Valk B.E., Brammar W.J.;
RT   ;
RL   Cloning Vectors 0:0-0(1985).
RL   Elsevier, North Holland.
XX
CC   NM (YRp12)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(S.cerevisiae)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (YRp7)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda 590 HindIII
FT                   2. pMB1068 HindIII-HindIII 1166bp, URA3 gene
FT                   \ = YEp24 HindIII-HindIII 1166bp 2273..3439
FT                   -> lambda 590-Sc2904
FT                   1. YRp7 EcoRI-EcoRI 1454bp 2..1456, ARS/TRP1 gene
FT                   2. YIp5 EcoRI 5541bp 2..2
FT                   -> YRp12 6798bp
FT                   1. YRp7 AvaI 5738bp 1356..1356
FT                   dC tailing
FT                   2. lambda 590-Sc2904 HindIII-HindIII 1166bp, URA3 gene
FT                   \ = YEp24 HindIII-HindIII 1166bp 2273..3439
FT                   dG tailing
FT                   -> YRp12 6798bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             86..1276
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             1591..2394
FT                   /note="ANT yeast URA3 gene (uracil)"
FT   CDS             complement(4355..5215)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      complement(5426..6261)
FT                   /note="ORI yeast ARS1"
FT   promoter        complement(6102..6773)
FT                   /note="PRO yeast TRP1 gene"
XX
SQ   Sequence 6798 BP; 1736 A; 1699 C; 1650 G; 1713 T; 0 other;
     agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga
     gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc
     acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc
     gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
     acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca
     atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg
     cgtccgccat ctccagcagc cgcacgcggc gcatcccccc cccccattcg gtaatctccg
     aacagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatatgta
     gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga acaaaaacct
     gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct gctactcatc
     ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg
     cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca
     aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag
     ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa gacagaaaat
     ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag
     aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga
     agcaggcggc agaagaagta acaaaggaac ctagaggcct tttgatgtta gcagaattgt
     catgcaaggg ctccctatct actggagaat atactaaggg tactgttgac attgcgaaga
     gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag
     gttacgattg gttgattatg acacccggtg tgggtttaga tgacaaggga gacgcattgg
     gtcaacagta tagaaccgtg gatgatgtgg tctctacagg atctgacatt attattgttg
     gaagaggact atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag
     caggctggga agcatatttg agaagatgcg gccagcaaaa ctaaaaaact gtattataag
     taaatgcatg tatactaaac tcacaaatta gagcttcaat ttaattatat cagttattac
     ccgggccccc ccccctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc
     ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca
     ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca
     acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc
     tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct
     acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc
     atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca
     gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga
     aattccccct tacacggagg catcaagtga ccaaacagga aaaaaccgcc cttaacatgg
     cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg
     atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct
     gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     tcgtcttcaa gaattcatgt taaaatagtg aaggagcatg ttcggcacac agtggaccga
     acgtggggta agtgcactag ggtccggtta aacggatctc gcattgatga ggcaacgcta
     attatcaaca tatagattgt tatctatctg catgaacacg aaatctttac ttgacgactt
     gaggctgatg gtgtttatgc aaagaaacca ctgtgtttaa tatgtgtcac tgtttgatat
     tactgtcagc gtagaagata atagtaaaag cggttaataa gtgtatttga gataagtgtg
     ataaagtttt tacagcgaaa agacgataaa tacaagaaaa tgattacgag gatacggaga
     gaggtatgta catgtgtatt tatatactaa gctgccggcg gttgtttgca agaccgagaa
     aaggctagca agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag
     tttgattcca ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata ttttcctgta
     caatcaatca aaaagccaaa tgatttagca ttatctttac atcttgttat tttacagatt
     ttatgtttag atcttttatg cttgcttttc aaaaggcctg caggcaagtg cacaaacaat
     acttaaataa atactactca gtaataacct atttcttagc atttttgacg aaatttgcta
     ttttgttaga gtcttttaca ccatttgtct ccacacctcc gcttacatca acaccaataa
     cgccatttaa tctaagcgca tcaccaacat tttctggcgt cagtccacca gctaacataa
     aatgtaagct ttcggggctc tcttgccttc caacccagtc agaaatcgag ttccaatcca
     aaagttcacc tgtcccacct gcttctgaat caaacaaggg aataaacgaa tgaggtttct
     gtgaagctgc actgagtagt atgttgcagt cttttggaaa tacgagtctt ttaataactg
     gcaaaccgag gaactcttgg tattcttgcc acgactcatc tccatgcagt tggacgatat
     caatgccgta atcattgacc agagccaaaa catcctcctt aggttgatta cgaaacacgc
     caaccaagta tttcggagtg cctgaactat ttttatatgc ttttacaaga cttgaaattt
     tccttgcaat aaccgggtca attgttctct ttctattggg cacacatata atacccagca
     agtcagcatc ggaatctaga gcacattctg cggcctctgt gctctgcaag ccgcaaactt
     tcaccaatgg accagaacta cctgtgaaat taataacaga catactccaa gctgcctttg
     tgtgcttaat cacgtatact cacgtgctca atagtcacca atgccctccc tcttggccct
     ctccttttct tttttgaa
//