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ID   YRP17      preliminary; circular DNA; SYN; 7002 BP.
XX
AC   L09158; VB0069; ATCC37078;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector YRp17 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   YRp17 from YRp12
RC   YC2p1 or pNN244 from YRp17 or pNN122 & CEN4 or pNN130
RC   YC2p2 or pNN245 from YRp17 or pNN122 & CEN4 or pNN130
RC   YC2p3 or pNN246 from YRp17 or pNN122 & CEN4 or pNN130
RC   pNN220 series, pNN230 series, pNN240 series from YC2p series
RA   Mann C., Davis R.W.;
RT   "Instability of dicentric plasmids in yeast";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:228-232(1983).
XX
RN   [2]
RP   1-7002
RC   YRp17 or pNN122
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
RN   [3]
RC   [from none]
RA   Botstein D., Davis R.W.;
RT   ;
RL   The Molecular Biology of the Yeast Saccharomyces 0:607-636(1982).
RL   Strathan et al, eds., Cold Spring Harbor Laboratory.
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   The length of G- or C-tails used in the cloning procedure is
CC   not exactly known. Therefore the sequencea 2890-2899, C(10)
CC   and 4062-4071 G(10) are tentative.
CC   YRp17 differs from YRp12 by the loss of the EcoRI site adjacent to the
CC   ampR gene.  YRp12 was constructed from YIp5 (ATCC 37061) by inserting
CC   a 1.45 kb EcoRI TRP1-ARS1 fragment. [2]
CC   YRp17 transforms yeast at high frequencies and is mitotically
CC   unstable. [2]
CC   A general purpose replicating Saccharomyces cerevisiae/E.coli
CC   shuttle vector. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   old New England Biolabs (NEB) vector.
CC   NM (YRp17)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli BNN45)(Saccharomyces)(E.coli MM294)(E.coli)
CC   HO (E.coli C600)(S.cerevisiae SHY series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. YRp12 EcoRI 6798bp 5352..5352, next to tet
FT                   fill in
FT                   -> YRp17 7002bp"
FT   misc_binding    0..0
FT                   /note="SIT unique ClaI-BamHI-SalI-SmaI-BalI-PvuII"
FT   misc_feature    6..1458
FT                   /note="from Trp1-ARS1 region (GenBank YSCTRP1)"
FT   CDS             108..782
FT                   /note="ANT yeast TRP1 gene"
FT   rep_origin      620..1458
FT                   /note="ORI yeast ARS"
FT   misc_feature    1465..2889
FT                   /note="from pBR325"
FT   misc_feature    2890..2899
FT                   /note="G-tail (assumed size)"
FT   CDS             2900..4061
FT                   /note="ANT yeast URA3 gene;
FT                   GenBank YSCODCD"
FT   misc_feature    complement(4062..4071)
FT                   /note="G-tail (assumed size)"
FT   misc_feature    4070..7002
FT                   /note="from pBR325"
FT   CDS             1550..2740
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(5940..6728)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             3122..3925
FT                   /note="ANT yeast URA3 gene (+D4)"
XX
SQ   Sequence 7002 BP; 1804 A; 1678 C; 1724 G; 1796 T; 0 other;
     gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
     tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
     atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
     cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
     agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
     atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
     ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
     ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
     actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
     attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
     gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgcctg caggcctttt
     gaaaagcaag cataaaagat ctaaacataa aatctgtaaa ataacaagat gtaaagataa
     tgctaaatca tttggctttt tgattgattg tacaggaaaa tatacatcgc agggggttga
     cttttaccat ttcaccgcaa tggaatcaaa cttgttgaag agaatgttca caggcgcata
     cgctacaatg acccgattct tgctagcctt ttctcggtct tgcaaacaac cgccggcagc
     ttagtatata aatacacatg tacatacctc tctccgtatc ctcgtaatca ttttcttgta
     tttatcgtct tttcgctgta aaaactttat cacacttatc tcaaatacac ttattaaccg
     cttttactat tatcttctac gctgacagta atatcaaaca gtgacacata ttaaacacag
     tggtttcttt gcataaacac catcagcctc aagtcgtcaa gtaaagattt cgtgttcatg
     cagatagata acaatctata tgttgataat tagcgttgcc tcatcaatgc gagatccgtt
     taaccggacc ctagtgcact taccccacgt tcggtccact gtgtgccgaa catgctcctt
     cactatttta acatgtggaa ttaattctca tgtttgacag cttatcatcg ataagcttta
     atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa
     caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt
     tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca
     ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg
     agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac
     tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atcctctacg ccggacgcat
     cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     gcataaggga gagcgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag
     cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc
     cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat
     ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt
     ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct
     gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac
     cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg cctcggcgag
     cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc
     gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc
     gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat
     gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg
     cggcgcatcc ccccccccct ttcaattcaa ttcatcattt tttttttatt cttttttttg
     atttcggttt ctttgaaatt tttttgattc ggtaatctcc gaacagaagg aagaacgaag
     gaaggagcac agacttagat tggtatatat acgcatatgt agtgttgaag aaacatgaaa
     ttgcccagta ttcttaaccc aactgcacag aacaaaaacc tgcaggaaac gaagataaat
     catgtcgaaa gctacatata aggaacgtgc tgctactcat cctagtcctg ttgctgccaa
     gctatttaat atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac
     caccaaggaa ttactggagt tagttgaagc attaggtccc aaaatttgtt tactaaaaac
     acatgtggat atcttgactg atttttccat ggagggcaca gttaagccgc taaaggcatt
     atccgccaag tacaattttt tactcttcga agacagaaaa tttgctgaca ttggtaatac
     agtcaaattg cagtactctg cgggtgtata cagaatagca gaatgggcag acattacgaa
     tgcacacggt gtggtgggcc caggtattgt tagcggtttg aagcaggcgg cagaagaagt
     aacaaaggaa cctagaggcc ttttgatgtt agcagaattg tcatgcaagg gctccctatc
     tactggagaa tatactaagg gtactgttga cattgcgaag agcgacaaag attttgttat
     cggctttatt gctcaaagag acatgggtgg aagagatgaa ggttacgatt ggttgattat
     gacacccggt gtgggtttag atgacaaggg agacgcattg ggtcaacagt atagaaccgt
     ggatgatgtg gtctctacag gatctgacat tattattgtt ggaagaggac tatttgcaaa
     gggaagggat gctaaggtag agggtgaacg ttacagaaaa gcaggctggg aagcatattt
     gagaagatgc ggccagcaaa actaaaaaac tgtattataa gtaaatgcat gtatactaaa
     ctcacaaatt agagcttcaa tttaattata tcagttatta cccgggaatc tcggtcgtaa
     tgatttttat aatgacgaaa aaaaaaaaat tggaaagaaa aggggggggg ggcagcgttg
     ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc
     ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact
     gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt
     cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg
     caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt
     gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac
     aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc
     gtttcatcgg tatcattacc cccatgaaca gaaattcccc cttacacgga ggcatcaagt
     gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac
     gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct
     tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga
     aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     acctataaaa ataggcgtat cacgaggccc tttcgtcttc aa
//