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ID YRP17 preliminary; circular DNA; SYN; 7002 BP.
XX
AC L09158; VB0069; ATCC37078;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Saccharomyces/E.coli plasmid vector YRp17 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC YRp17 from YRp12
RC YC2p1 or pNN244 from YRp17 or pNN122 & CEN4 or pNN130
RC YC2p2 or pNN245 from YRp17 or pNN122 & CEN4 or pNN130
RC YC2p3 or pNN246 from YRp17 or pNN122 & CEN4 or pNN130
RC pNN220 series, pNN230 series, pNN240 series from YC2p series
RA Mann C., Davis R.W.;
RT "Instability of dicentric plasmids in yeast";
RL Proc. Natl. Acad. Sci. U.S.A. 80:228-232(1983).
XX
RN [2]
RP 1-7002
RC YRp17 or pNN122
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
XX
RN [3]
RC [from none]
RA Botstein D., Davis R.W.;
RT ;
RL The Molecular Biology of the Yeast Saccharomyces 0:607-636(1982).
RL Strathan et al, eds., Cold Spring Harbor Laboratory.
XX
CC These data and their annotation were supplied to GenBank by Will
CC Gilbert under the auspices of the GenBank Currator Program.
CC
CC The length of G- or C-tails used in the cloning procedure is
CC not exactly known. Therefore the sequencea 2890-2899, C(10)
CC and 4062-4071 G(10) are tentative.
CC YRp17 differs from YRp12 by the loss of the EcoRI site adjacent to the
CC ampR gene. YRp12 was constructed from YIp5 (ATCC 37061) by inserting
CC a 1.45 kb EcoRI TRP1-ARS1 fragment. [2]
CC YRp17 transforms yeast at high frequencies and is mitotically
CC unstable. [2]
CC A general purpose replicating Saccharomyces cerevisiae/E.coli
CC shuttle vector. (ATCC staff)
CC Medium is 1227 LB plus ampicillin.
CC old New England Biolabs (NEB) vector.
CC NM (YRp17)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli BNN45)(Saccharomyces)(E.coli MM294)(E.coli)
CC HO (E.coli C600)(S.cerevisiae SHY series)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. YRp12 EcoRI 6798bp 5352..5352, next to tet
FT fill in
FT -> YRp17 7002bp"
FT misc_binding 0..0
FT /note="SIT unique ClaI-BamHI-SalI-SmaI-BalI-PvuII"
FT misc_feature 6..1458
FT /note="from Trp1-ARS1 region (GenBank YSCTRP1)"
FT CDS 108..782
FT /note="ANT yeast TRP1 gene"
FT rep_origin 620..1458
FT /note="ORI yeast ARS"
FT misc_feature 1465..2889
FT /note="from pBR325"
FT misc_feature 2890..2899
FT /note="G-tail (assumed size)"
FT CDS 2900..4061
FT /note="ANT yeast URA3 gene;
FT GenBank YSCODCD"
FT misc_feature complement(4062..4071)
FT /note="G-tail (assumed size)"
FT misc_feature 4070..7002
FT /note="from pBR325"
FT CDS 1550..2740
FT /note="ANT E. coli tetracycline resistance gene (tet)"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(5940..6728)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 3122..3925
FT /note="ANT yeast URA3 gene (+D4)"
XX
SQ Sequence 7002 BP; 1804 A; 1678 C; 1724 G; 1796 T; 0 other;
gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgcctg caggcctttt
gaaaagcaag cataaaagat ctaaacataa aatctgtaaa ataacaagat gtaaagataa
tgctaaatca tttggctttt tgattgattg tacaggaaaa tatacatcgc agggggttga
cttttaccat ttcaccgcaa tggaatcaaa cttgttgaag agaatgttca caggcgcata
cgctacaatg acccgattct tgctagcctt ttctcggtct tgcaaacaac cgccggcagc
ttagtatata aatacacatg tacatacctc tctccgtatc ctcgtaatca ttttcttgta
tttatcgtct tttcgctgta aaaactttat cacacttatc tcaaatacac ttattaaccg
cttttactat tatcttctac gctgacagta atatcaaaca gtgacacata ttaaacacag
tggtttcttt gcataaacac catcagcctc aagtcgtcaa gtaaagattt cgtgttcatg
cagatagata acaatctata tgttgataat tagcgttgcc tcatcaatgc gagatccgtt
taaccggacc ctagtgcact taccccacgt tcggtccact gtgtgccgaa catgctcctt
cactatttta acatgtggaa ttaattctca tgtttgacag cttatcatcg ataagcttta
atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa
caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt
tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca
ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg
agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac
tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atcctctacg ccggacgcat
cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
gcataaggga gagcgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag
cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc
cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat
ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt
ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct
gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac
cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg cctcggcgag
cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc
gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc
gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat
gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg
cggcgcatcc ccccccccct ttcaattcaa ttcatcattt tttttttatt cttttttttg
atttcggttt ctttgaaatt tttttgattc ggtaatctcc gaacagaagg aagaacgaag
gaaggagcac agacttagat tggtatatat acgcatatgt agtgttgaag aaacatgaaa
ttgcccagta ttcttaaccc aactgcacag aacaaaaacc tgcaggaaac gaagataaat
catgtcgaaa gctacatata aggaacgtgc tgctactcat cctagtcctg ttgctgccaa
gctatttaat atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac
caccaaggaa ttactggagt tagttgaagc attaggtccc aaaatttgtt tactaaaaac
acatgtggat atcttgactg atttttccat ggagggcaca gttaagccgc taaaggcatt
atccgccaag tacaattttt tactcttcga agacagaaaa tttgctgaca ttggtaatac
agtcaaattg cagtactctg cgggtgtata cagaatagca gaatgggcag acattacgaa
tgcacacggt gtggtgggcc caggtattgt tagcggtttg aagcaggcgg cagaagaagt
aacaaaggaa cctagaggcc ttttgatgtt agcagaattg tcatgcaagg gctccctatc
tactggagaa tatactaagg gtactgttga cattgcgaag agcgacaaag attttgttat
cggctttatt gctcaaagag acatgggtgg aagagatgaa ggttacgatt ggttgattat
gacacccggt gtgggtttag atgacaaggg agacgcattg ggtcaacagt atagaaccgt
ggatgatgtg gtctctacag gatctgacat tattattgtt ggaagaggac tatttgcaaa
gggaagggat gctaaggtag agggtgaacg ttacagaaaa gcaggctggg aagcatattt
gagaagatgc ggccagcaaa actaaaaaac tgtattataa gtaaatgcat gtatactaaa
ctcacaaatt agagcttcaa tttaattata tcagttatta cccgggaatc tcggtcgtaa
tgatttttat aatgacgaaa aaaaaaaaat tggaaagaaa aggggggggg ggcagcgttg
ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc
ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact
gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt
cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg
caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt
gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac
aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc
gtttcatcgg tatcattacc cccatgaaca gaaattcccc cttacacgga ggcatcaagt
gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac
gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct
tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga
aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
acctataaaa ataggcgtat cacgaggccc tttcgtcttc aa
//