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ID   YRP7       preliminary; circular DNA; SYN; 5817 BP.
XX
AC   U03501; IG0132; ATCC37060;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector YRp7 - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-5817
RC   pGT2-Sc2605 from yeast HIS3 gene
RC   pBR322-Sc2676 from pBR322 & yeast HIS3 gene
RC   lambda 590-Sc2904 from lambda 590 & pMB1068, yeast URA3 gene
RC   lambda gt-Sc4104 from lambda gt10 & yeast ARS1/TRP1 gene
RC   pT322 from pBR322 & yeast ARS1/TRP1 gene 1.4kb EcoRI-EcoRI
RC   YIp1 from pBR322 & pGT2-Sc2605
RC   YIp5 or pNN36 from pBR322 & lambda 590-Sc2904
RC   YEp2 from pBR322 & SCP1
RC   YEp4 from pBR322 & lambda & SCP1
RC   YEp6 from pBR322-Sc2676 & SCP1
RC   YRp7 or pNN78 from pBR322 & lambda gt-Sc4104
RC   [YEp-3A, YEp-3B from pBR322 & yeast 2 micron]
RC   [YRp12 from YRp series]
RA   Struhl K., Stinchcomb D.T., Scherer S., Davis R.W.;
RT   "High-frequency transformation of yeast: autonomous replication of
RT   hybrid DNA molecules";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:1035-1039(1979).
XX
RN   [2]
RC   YRp7, YRp7' from pBR322 & lambda gt-Sc4101, ARS1/TRP1 gene
RC   YRp7-Sc2605, YRp7'-Sc2605 from YIp1 & lambda gt-Sc4101
RC   Yp411, Yp412, Yp413, Yp414 from YRp7
RA   Stinchcomb D.T., Struhl K., Davis R.W.;
RT   "Isolation and characterisation of a yeast chromosomal replicator";
RL   Nature 282:39-43(1979).
XX
RN   [3]
RP   1-5817
RC   YRp7
RA   Stillman D.J.;
RT   ;
RL   Submitted (16-NOV-1993) by:
RL   Stillman D.J., Dept. of Cellular, Viral and Molecular Biology,
RL   University of Utah Medical Center, Salt Lake City, UT 84132 USA.
XX
CC   YRp7 was constructed by inserting a 1.4 kb EcoRI fragment containing
CC   TRP1 and ARS1 from lambda gt-Sc4101 into pBR322.  The ARS1 fragment is
CC   in a 0.85 kb EcoRI-HindIII subfragment. [5]
CC   A general purpose replicating Saccharomyces cerevisiae/Escherichia
CC   coli shuttle vector. [4]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (YRp7)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli K-12 r-m+)(E.coli C600)(E.coli MM294)(E.coli)
CC   HO (S.cerevisiae)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF (YRp12)(pYe[MET14]2)(pYe[MET14]27)(pYe[CEN11]12)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda gt11 EcoRI 43700bp
FT                   shearing
FT                   2. yeast EcoRI-EcoRI 1454bp, ARS1/TRP1 gene
FT                   shearing
FT                   -> lambda gtSc4101 45100bp
FT                   1. pBR322 EcoRI 4361bp 4360..4360
FT                   2. lambda gtSc4101 EcoRI-EcoRI 1454bp, ARS1/TRP1 gene
FT                   -> YRp7 5738bp [TRP1 EcoRI-EcoRI 1454bp 2..1456]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene (uracil)"
FT   CDS             86..1276
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             complement(3295..4155)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      complement(4366..5201)
FT                   /note="ORI yeast ARS1"
FT   promoter        complement(5042..5713)
FT                   /note="PRO yeast TRP1 gene"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
XX
SQ   Sequence 5817 BP; 1415 A; 1524 C; 1408 G; 1470 T; 0 other;
     gaattcccac atgttaaaat agtgaaggag catgttcggc acacagtgga ccgaacgtgg
     ggtaagtgca ctagggtccg gttaaacgga tctcgcattg atgaggcaac gctaattatc
     aacatataga ttgttatcta tctgcatgaa cacgaaatct ttacttgacg acttgaggct
     gatggtgttt atgcaaagaa accactgtgt ttaatatgtg tcactgtttg atattactgt
     cagcgtagaa gataatagta aaagcggtta ataagtgtat ttgagataag tgtgataaag
     tttttacagc gaaaagacga taaatacaag aaaatgatta cgaggatacg gagagaggta
     tgtacatgtg tatttatata ctaagctgcc ggcggttgtt tgcaagaccg agaaaaggct
     agcaagaatc gggtcattgt agcgtatgcg cctgtgaaca ttctcttcaa caagtttgat
     tccattgcgg tgaaatggta aaagtcaacc ccctgcgatg tatattttcc tgtacaatca
     atcaaaaagc caaatgattt agcattatct ttacatcttg ttattttaca gattttatgt
     ttagatcttt tatgcttgct tttcaaaagg cctgcaggca agtgcacaaa caatacttaa
     ataaatacta ctcagtaata acctatttct tagcattttt gacgaaattt gctattttgt
     tagagtcttt tacaccattt gtctccacac ctccgcttac atcaacacca ataacgccat
     ttaatctaag cgcatcacca acattttctg gcgtcagtcc accagctaac ataaaatgta
     agctttcggg gctctcttgc cttccaaccc agtcagaaat cgagttccaa tccaaaagtt
     cacctgtccc acctgcttct gaatcaaaca agggaataaa cgaatgaggt ttctgtgaag
     ctgcactgag tagtatgttg cagtcttttg gaaatacgag tcttttaata actggcaaac
     cgaggaactc ttggtattct tgccacgact catctccatg cagttggacg atatcaatgc
     cgtaatcatt gaccagagcc aaaacatcct ccttaggttg attacgaaac acgccaacca
     agtatttcgg agtgcctgaa ctatttttat atgcttttac aagacttgaa attttccttg
     caataaccgg gtcaattgtt ctctttctat tgggcacaca tataataccc agcaagtcag
     catcggaatc tagagcacat tctgcggcct ctgtgctctg caagccgcaa actttcacca
     atggaccaga actacctgtg aaattaataa cagacatact ccaagctgcc tttgtgtgct
     taatcacgta tactcacgtg ctcaatagtc accaatgccc tccctcttgg ccctctcctt
     ttcttttttc gaccgaattc tcatgtttga cagcttatca tcgataagct ttaatgcggt
     agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg
     ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg
     gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc
     gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg
     tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac
     tacgcgatca tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc
     ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
     gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta
     acttcgatca ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg
     aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt
     cgcggtgcat ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg
     gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa
     ccaacccttg gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca
     tctcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga
     cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc
     gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct
     tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt
     tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa
     cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca
     gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg
     tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat tcccccttac
     acggaggcat caagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag
     aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga
     catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt
     cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
     gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
     cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaa
//