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ID YRP7 preliminary; circular DNA; SYN; 5817 BP.
XX
AC U03501; IG0132; ATCC37060;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Saccharomyces/E.coli plasmid vector YRp7 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-5817
RC pGT2-Sc2605 from yeast HIS3 gene
RC pBR322-Sc2676 from pBR322 & yeast HIS3 gene
RC lambda 590-Sc2904 from lambda 590 & pMB1068, yeast URA3 gene
RC lambda gt-Sc4104 from lambda gt10 & yeast ARS1/TRP1 gene
RC pT322 from pBR322 & yeast ARS1/TRP1 gene 1.4kb EcoRI-EcoRI
RC YIp1 from pBR322 & pGT2-Sc2605
RC YIp5 or pNN36 from pBR322 & lambda 590-Sc2904
RC YEp2 from pBR322 & SCP1
RC YEp4 from pBR322 & lambda & SCP1
RC YEp6 from pBR322-Sc2676 & SCP1
RC YRp7 or pNN78 from pBR322 & lambda gt-Sc4104
RC [YEp-3A, YEp-3B from pBR322 & yeast 2 micron]
RC [YRp12 from YRp series]
RA Struhl K., Stinchcomb D.T., Scherer S., Davis R.W.;
RT "High-frequency transformation of yeast: autonomous replication of
RT hybrid DNA molecules";
RL Proc. Natl. Acad. Sci. U.S.A. 76:1035-1039(1979).
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RN [2]
RC YRp7, YRp7' from pBR322 & lambda gt-Sc4101, ARS1/TRP1 gene
RC YRp7-Sc2605, YRp7'-Sc2605 from YIp1 & lambda gt-Sc4101
RC Yp411, Yp412, Yp413, Yp414 from YRp7
RA Stinchcomb D.T., Struhl K., Davis R.W.;
RT "Isolation and characterisation of a yeast chromosomal replicator";
RL Nature 282:39-43(1979).
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RN [3]
RP 1-5817
RC YRp7
RA Stillman D.J.;
RT ;
RL Submitted (16-NOV-1993) by:
RL Stillman D.J., Dept. of Cellular, Viral and Molecular Biology,
RL University of Utah Medical Center, Salt Lake City, UT 84132 USA.
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CC YRp7 was constructed by inserting a 1.4 kb EcoRI fragment containing
CC TRP1 and ARS1 from lambda gt-Sc4101 into pBR322. The ARS1 fragment is
CC in a 0.85 kb EcoRI-HindIII subfragment. [5]
CC A general purpose replicating Saccharomyces cerevisiae/Escherichia
CC coli shuttle vector. [4]
CC Medium is 1227 LB plus ampicillin.
CC NM (YRp7)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli K-12 r-m+)(E.coli C600)(E.coli MM294)(E.coli)
CC HO (S.cerevisiae)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pBR322)
CC BR ()
CC OF (YRp12)(pYe[MET14]2)(pYe[MET14]27)(pYe[CEN11]12)
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. lambda gt11 EcoRI 43700bp
FT shearing
FT 2. yeast EcoRI-EcoRI 1454bp, ARS1/TRP1 gene
FT shearing
FT -> lambda gtSc4101 45100bp
FT 1. pBR322 EcoRI 4361bp 4360..4360
FT 2. lambda gtSc4101 EcoRI-EcoRI 1454bp, ARS1/TRP1 gene
FT -> YRp7 5738bp [TRP1 EcoRI-EcoRI 1454bp 2..1456]"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="ANT yeast URA3 gene (uracil)"
FT CDS 86..1276
FT /note="ANT E. coli tetracycline resistance gene (tet)"
FT CDS complement(3295..4155)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin complement(4366..5201)
FT /note="ORI yeast ARS1"
FT promoter complement(5042..5713)
FT /note="PRO yeast TRP1 gene"
FT misc_binding 0..0
FT /note="SIT unique BamHI"
FT misc_binding 0..0
FT /note="SIT unique SalI"
XX
SQ Sequence 5817 BP; 1415 A; 1524 C; 1408 G; 1470 T; 0 other;
gaattcccac atgttaaaat agtgaaggag catgttcggc acacagtgga ccgaacgtgg
ggtaagtgca ctagggtccg gttaaacgga tctcgcattg atgaggcaac gctaattatc
aacatataga ttgttatcta tctgcatgaa cacgaaatct ttacttgacg acttgaggct
gatggtgttt atgcaaagaa accactgtgt ttaatatgtg tcactgtttg atattactgt
cagcgtagaa gataatagta aaagcggtta ataagtgtat ttgagataag tgtgataaag
tttttacagc gaaaagacga taaatacaag aaaatgatta cgaggatacg gagagaggta
tgtacatgtg tatttatata ctaagctgcc ggcggttgtt tgcaagaccg agaaaaggct
agcaagaatc gggtcattgt agcgtatgcg cctgtgaaca ttctcttcaa caagtttgat
tccattgcgg tgaaatggta aaagtcaacc ccctgcgatg tatattttcc tgtacaatca
atcaaaaagc caaatgattt agcattatct ttacatcttg ttattttaca gattttatgt
ttagatcttt tatgcttgct tttcaaaagg cctgcaggca agtgcacaaa caatacttaa
ataaatacta ctcagtaata acctatttct tagcattttt gacgaaattt gctattttgt
tagagtcttt tacaccattt gtctccacac ctccgcttac atcaacacca ataacgccat
ttaatctaag cgcatcacca acattttctg gcgtcagtcc accagctaac ataaaatgta
agctttcggg gctctcttgc cttccaaccc agtcagaaat cgagttccaa tccaaaagtt
cacctgtccc acctgcttct gaatcaaaca agggaataaa cgaatgaggt ttctgtgaag
ctgcactgag tagtatgttg cagtcttttg gaaatacgag tcttttaata actggcaaac
cgaggaactc ttggtattct tgccacgact catctccatg cagttggacg atatcaatgc
cgtaatcatt gaccagagcc aaaacatcct ccttaggttg attacgaaac acgccaacca
agtatttcgg agtgcctgaa ctatttttat atgcttttac aagacttgaa attttccttg
caataaccgg gtcaattgtt ctctttctat tgggcacaca tataataccc agcaagtcag
catcggaatc tagagcacat tctgcggcct ctgtgctctg caagccgcaa actttcacca
atggaccaga actacctgtg aaattaataa cagacatact ccaagctgcc tttgtgtgct
taatcacgta tactcacgtg ctcaatagtc accaatgccc tccctcttgg ccctctcctt
ttcttttttc gaccgaattc tcatgtttga cagcttatca tcgataagct ttaatgcggt
agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg
ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg
gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc
gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg
tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac
tacgcgatca tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc
ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta
acttcgatca ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg
aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt
cgcggtgcat ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg
gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa
ccaacccttg gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca
tctcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga
cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc
gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct
tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt
tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa
cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca
gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg
tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat tcccccttac
acggaggcat caagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag
aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga
catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt
cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaa
//