cosKT1


Vector IG Sequence Link :
General : cosmid ds-DNA 5200 BP
Functions : (cloning 35000-45000)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha)(E.coli)
Suppliers : (ATCC)
Misc.Comments : cos4/pcos4EMBL was digested with BamHI+AvaI, the 1.1 kb fragment removed and the remaining ends filled in. Synthetic NotI linkers were inserted into the EcoRI & HindIII sites. The small fragment between EcoRI & HindIII was replaced with the pUC18 polylinker. Cosmid clones contain either 3.2 or 2.7 kb of the original vector depending on which cos site was used in packaging. This does not affect the use of NotI sites in restriction mapping of the clone. Cosmid vector with a cloning capacity of 35-45 kb, 3 cos sites, and NotI sites flanking the polylinker sites for ease of restriction mapping. Restriction digests of the clone give the following sizes (kb): EcoRI--5.3; BamHI--5.3; PstI--4.30, 0.8. (ATCC staff) PATENT PENDING. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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