EBOpLPP


Vector IG Sequence Link :
General : plasmid ds-DNA 10572 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)(human cell line)(simian cell line)(dog cell line)
Suppliers : (ATCC)
Misc.Comments : This was also constructed from the SfiI fragment of EBOpSV2neo (hygromycin phosphotransferase gene, oriP, and EBNA-1 gene). [1] This is a shuttle vector permitting expression in primate cell lines. It is useful for subcloning. [2] This was constructed from the HindIII/EcoRI fragment of pcDV (pBR322 ori, beta-lactamase, SV40 late polyadenylation signal) and the HindIII/PstI fragment of pL (SV40 early promoter and SV40 late intervening sequence). [1] Restriction digests of the clone give the following sizes (kb): HindIII/EcoRI--7.0, 3.5, 0.6; EcoRI--7.0, 4.0; SalI--10.6; XbaI--10.6. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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