KS+SV0CAT


Vector IG Sequence Link :
General : plasmid ds-DNA 4841 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. HindIII-ApaI fragment from pSV2cat contains internal BamHI and EcoRI sites. Mammalian shuttle expression vector for promoter cloning with activity analysis by CAT (chloramphenicol acetyl transferase) assay. Use KS+SV2CAT (ATCC 37609) as a control. Restriction digests of the clone give the following sizes (kb): SacI--4.4; Xba--4.4; BamHI--2.9, 1.6; SmaI--4.4; PstI--4.4; EcoRI--3.9, 0.32; HindIII--4.4. (ATCC staff) Constructed by inserting the HindIII-ApaI fragment of pSV2cat in HindIII-ApaI digested Bluescript KS+. Contains no SV40 promoter. (personal communication) Medium is 1227 LB plus ampicillin.
Parents : (pBluescript KS+)(pSV2-cat)
Siblings : ()
Descendents : ()


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