Vector IG Sequence Link :
General : plasmid ds-DNA 5373 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(vertebrate cells)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. Mammalian shuttle expression vector containing a CAT (chloramphenicol acetyl transferase) gene expressed under an SV40 promoter. To be used as a control for promoter activity in conjuction with the promoter cloning plasmid KS+SV0CAT (ATCC 37608). Additional polylinker sites are ApaI and KpnI. Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 2.1; SmaI--4.7; PstI--4.7; EcoRI--4.l, 0.72; ClaI--4.7. (ATCC staff) Constructed by inserting the AccI-ApaI fragment of pSV2cat into the EcoRV site of Bluescript KS+. Ends were blunt. (personal communication) Medium is 1227 LB plus ampicillin.
Parents : (pBluescript KS+)(pSV2-cat)
Siblings : ()
Descendents : ()

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