lambda 2001


Vector IG Sequence Link :
General : phage ds-DNA 41900 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (bacteria-free lysate)(E.coli)(E.coli LE392 [for propagation])(E.coli NM539 [for recombinant])
Suppliers : (ATCC)
Misc.Comments : Rehydrate in 1 ml 10 mM magnesium sulphate. Recombinants are Spi- cI- Gam- Int- Red-. A lambda replacement vector carrying a polylinker sequence with positive selection for large (10-23 kb) inserts.) Genotype of vector is lambdasbhI lambda1(0)b189 SIGMAint (linker) srIlambda3(0) ninL44 SIGMAshndIII lambda4 (bio) (linker) delta(sbhIlambda3-sbhIlambda4) KH54 srIlambda4(0) chiC nin5 srIlambda5(0) shndIIIlambda6(0). The arms are 20.1 kb and 9.4 kb. (ATCC staff) Medium is 1592 SM buffer.
Parents : ()
Siblings : ()
Descendents : ()


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