Vector IG Sequence Link :
General : phage ds-DNA 48502 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : (USB)
Misc.Comments : References [3] to [85] are either annotated by [83] or [84]. References [83] to [84] are the sources for the annotations. Only references [25] through [84] are represented in the feature table. This is the best representation to date of the wild-type lambda l-strand, though much of the sequence was determined for the cI857s7 strain and changed to wild-type [83]. All reported variations leading to the strains cI857s7, imm21, imm434, lac5, Nin5, and b2 are included in the annotation. The first twelve bases are the sticky ends. A significant fraction of the known mutations affecting replication and transcription have been annotated [83]. A large number of point mutations, deletions and substitutions have not been annotated [83]. Each coding sequence belongs to a reading frame (ORF) whose number, in parentheses, should indicate the number of amino acids coded [73]. The starting points for translation are known with varying degrees of certainty; for example, the start site for the N protein, given here as 35438, may be downstream (on the complementary strand) at 35360 [73]. When direct empirical evidence such as mutation or amino acid sequence is lacking, the start point is said to be putative. Intergenic spaces in lambda are typically short and overlapping: the multiple reading frames (mult) range between a span of 1 and a span of 103. In most cases, a start codon precedes a termination codon, exceptions being the m-l boundary, at 13429, and the 314-194 boundary, at 21973, which show the E. coli trp operon pattern of translational coupling (see ECOTRP). Transcription in the central region, bases 22686 to 37940, is leftward off the l-strand. Signals and recognition sites in this region, without judgement made about their polarity, are treated accordingly, hence their span should be read toward the left rather than toward the right. Furthermore some leftward transcription is located outside the central region is also indicated on the complementary strand. In general, the estimates for the extent or span of signals like operators, binding sites like Nutr or int-binding sites, etc., and of the attachment site (att) vary in the literature [83]. No attempt is made to annotate promoters as signals because of the indefiniteness of their span. However, known promoter mutants are given. The cII protein is known to bind in the -35 regions of p-i (29091) and pre(38369). Transcript termination sites must be understood to be conditional on the N and Q proteins and less than 100% efficient. There remain terminators to be found and some of those annotated may have significance only in vitro.
Parents : (lambda wild-type)(lambda strain cI857s7)
Siblings : ()
Descendents : (lambda gt10)(lambda gt102)(lambda gt11)(lambda gt11D)(lambda gt11SN)(lambda gt22A)(lambda dvh93)(lambda 1059)(lambda 2001)(lambda amp3)(lambda spc2)(lambda dl10)(lambda dl11)(lambda DR2)(lambda JK2)(lambda JK4)(lambda cI857R)(lambda SE4)(lambda SE5)(lambda SE6)(lambda SHLX1)(lambda SHLX2)(lambda ZD31)(lambda ZD32)(lambda ZD35)(lambda pi 12)(lambda pi 25)(EMBL12)(EMBL3)(EMBL4)(Charon 4A)(Charon 28)(Charon 40)(Charomid 9-20)(Charomid 9-28)


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