lambda N- cI857 r32


Vector IG Sequence Link :
General : phage ds-DNA 1 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (bacteria-free lysate)(E.coli)(E.coli C600)
Suppliers : (ATCC)
Misc.Comments : This was isolated as spontaneous mutant of lambda papa prophage. It contains an IS2 terminator between the tR1 site and the O gene. This replicates in sup hosts (clear plaques at 42C; turbid plaques at 30C). It does not grow on sup0 hosts and does not kill a sup0 host when lysogenized and induced at 42C. (personal communication) This is bacteriophage lambda with mutant O and P genes regulated by the N product (functional only when N is supplied). It produces immunity to lambda. Genotype:Nam7 Nam53 cI857 r32 IS2 Medium is 1592 SM buffer.
Parents : ()
Siblings : ()
Descendents : ()


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