lambda ZD32


Vector IG Sequence Link :
General : phage ds-DNA 43700 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (bacteria-free lysate)(vertebrate cells)(E.coli)(E.coli RR1 pRK248 [ATCC 47036])(E.coli Wgd5 [CRL 1817])
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): EcoRI--18.5, 26.0. (ATCC staff) Same as lambdaZd31 but the tri-initiator is in the opposite direction. A cell line suitable for transfection by released plasmid is Wgd5, ATCC CRL 1817. The plasmid insert can be released by homologous recombination between the 2 LTRs after growth at low temperature in E.coli RRI(pRK248), ATCC 47036. Allows expression of cloned cDNA in mammalian cells; can be used for library transduction. Medium is 1592 SM buffer.
Parents : ()
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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