M13mp8


Vector IG Sequence Link :
General : phage filamentous ss-DNA)(ds-DNA 7229 BP
Functions : (cloning)(sequencing dideoxy)(in vitro mutagenesis)(labeled strand-specific DNA)
Selection : (color blue/white)
Copy Number :
Hosts : (E.coli JM101)(E.coli JM103)(E.coli JM105)(E.coli JM107)(E.coli JM109)(E.coli DH5alphaF')
Suppliers : (Amersham)(Boehringer)(Sigma)
Misc.Comments : M13 is not lytic; the phage extrude through the cell wall. The replicative RF I form in the cell is double-stranded and circular, and acts like a plasmid. The phage form outside the cell is linear and single-stranded. In vitro DNA synthesis on a cis-DDP-treated template generates a population of DNA fragments, indicating that duplex synthesis has terminated. Cis-DDP forms an adduct with DNA, at positions where multiple 'g' nucleotides occur, that inhibits synthesis in vitro. Trans DDP has the same effect, only on 'gng' sequences, where n can be any intervening nucleotide. NCBI gi: 215214 & 215215
Parents : (M13mp7)
Siblings : (M13mp9)
Descendents : (pUC8)(M13mp10)

NCBI ENTREZ Link :


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