M13tg117


Vector IG Sequence Link :
General : phage ds-DNA 63 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : ()
Misc.Comments : [1] reports inserting a HindIII linker into the 5' end of the beta galactosidase gene of phage M13, so that controlled expression of inserted coding sequences could be obtained. The expression of viral proteins in M13 does not seem detrimental to the host, as it is in E.coli. The spacing between EcoRI and BamHI sites was increased by 1 bp from that in clone M13tg110, producing a SmaI site. NCBI gi: 209452
Parents : (M13mp107)(M13)
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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