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ID   CASPER     preliminary; circular DNA; SYN; 7800 BP.
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AC   M28731;
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DT   23-SEP-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli vector CaSpeR - incomplete, DNA fragment.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-61
RC   pC4cat from pSV0cat & Carnegie 4
RC   plasmid from pSV2-dhfr & Carnegie 4
RC   pC4beta-gal from plasmid & pCGE41, lacZ gene
RC   pC4-AUG-betagal from pC4beta-gal & Drosophila ADH gene
RC   from pCaSpeR & pC4beta-gal
RC   from pCaSpeR & pC4-AUG-betagal
RC   pUChsneo-act from pUChsneo & pAMG12 & pDmA2, act5 gene
RC   from pUChsneo-act
RC   pC4HaeCat from pC4cat & rosy gene
RC   pC20HaeCat from pC4HaeCat & Carnegie 20 or car20
RC   [pCaSpeR from Drosophila P element]
RA   Thummel C.S., Boulet A.M., Lipshitz H.D.;
RT   "Vectors for Drosophila P-element-mediated transformation and tissue
RT   culture transfection";
RL   Gene 74:445-456(1988).
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RN   [2]
RC   pCaSpeR from Drosophila white gene & P element
RA   Pirrotta V.;
RT   "Vectors for P-mediated transformation in Drosophila";
RL   Vectors 0:437-456(1988).
RL   Rodriguez R.L., Denhardt D.T., eds. Butterworth, Boston.
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CC   NCBI gi: 341985
CC   NM (CaSpeR)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 2686bp
FT                   2. white gene and Drosophila P element
FT                   -> CaSpeR 7800bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-BamHI-KpnI-PstI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 61 BP; 11 A; 18 C; 21 G; 11 T; 0 other;
     aagcttgggc tgcaggtcga ctctagagga tcccgggcga gctcgaattc ccggggatcc
     c
//