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ID   CHARON40   preliminary; circular DNA; SYN; 28793 BP.
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AC   IG5144;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector Charon 40 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pUC19Bam- from pUC19
RC   plasmid from pUC19Bam- & pIN-II-A1
RC   phage from Charon 34 & plasmid
RC   Charon 35-H668 from Charon 35
RC   phage2 from Charon 35-H668 & linker
RC   Charon 36 from phage & phage2
RC   Charon 33 Sma- from Charon 33
RC   pUC8Sm/Xh from pUC8 & linker
RC   phage3 from Charon 33 & pUC8Sm/Xh
RC   Charon 37 from Charon 36 & phage3
RC   lambda FAC101 Apa'Kpn' from Charon 36 & lambda FAC101
RC   Charon 38A from Charon 37 & lambda FAC101 Apa'Kpn'
RC   Charon 38 from Charon 38A
RC   plasmid2 from pKC30 & pUC19 & adenovirus 2 & lac operator & linker
RC   pISD27 from pUC8 & oligo
RC   Charon 39A from plasmid2 & pISD27 & Charon 38
RC   Charon 39 from Charon 39A & Charon 38
RC   pISD34 from pBR322 & oligo
RC   Charon 40A from plasmid2 & pISD34 & Charon 39
RC   Charon 40 from Charon 40A & Charon 38
RA   Dunn I.S., Blattner F.R.;
RT   "Charons 36 to 40: multi enzyme, high capacity, recombination
RT   deficient replacement vectors with polylinkers and polystuffers";
RL   Nucleic Acids Res. 15:2677-2698(1987).
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RN   [2]
RC   phage from piAN7 & lambda
RC   lambda FAC101 from phage & Charon 30A
RA   Lawyer F., Gelfand D.;
RT   ;
RL   Unpublished (1987).
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RN   [3]
RC   [Charon 21A from Charon series]
RC   lambda BP1 from Charon 14 & Charon 21A
RC   lambda BVC4 from lambda BP1
RC   Charon 27 from Charon 21A & lambda BVC4
RC   lambda XDR3.24 from Charon 13A & Charon 10
RC   Charon 28 from lambda XDR3.24 & lambda BVC4
RC   Charon 28-2222 from Charon 28
RC   Charon 28-2270 from Charon 28
RC   Charon 30 from Charon 28-2222 & Charon 28-2270
RC   [Charon 30A from Charon 30]
RA   Rimm D.L., Horness D., Kucera J., Blattner F.R.;
RT   "Construction of coliphage lambda Charon vectors with BamHI
RT   cloning sites";
RL   Gene 12:301-309(1980).
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RN   [4]
RC   Charon 31 from Charon 30 & lambda BVC4
RC   Charon 32 from Charon 31 & mouse liver DNA
RC   Charon 33 from Charon 32
RC   pUC13Bam- from pUC13
RC   Charon 34 from Charon 33 & pUC13Bam- & pIN-II-A1
RC   Charon 35 from Charon 34
RA   Loenen W.A., Blattner F.R.;
RT   "Lambda Charon vectors (Ch32, 33, 34 and 35) adapted for DNA
RT   cloning in recombination-deficient hosts";
RL   Gene 26:171-179(1983).
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CC   MCS. oligonucleotide linker.
CC   NM (Charon 40)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Charon34)(Charon34)(lambda)
CC   BR (Charon36)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> Charon 10 [lambda lac5 deltaH3 bio256 KH54 nin5]
FT                   1. lambda
FT                   -> Charon 13A [lambda b1007 KH54 nin5 QSR imm80]
FT                   1. lambda
FT                   -> Charon 14 [lambda lac5 b1007 KH54 nin5 QSR imm80]
FT                   1. Charon 21
FT                   -> Charon 21A [lambda b1007 imm80 KH53 KH52 nin5]
FT                   1. Charon 14
FT                   cross with
FT                   2. Charon 21A
FT                   -> lambda BP1 [lambda lac5 b1007 imm80 KH53 KH52 nin5]
FT                   1. lambda BP1
FT                   mutant
FT                   -> lambda BVC4 [no BamHI 5' to lac5]
FT                   1. Charon 21A
FT                   cross with
FT                   2. lambda BVC4
FT                   -> Charon 27 [lambda b1007 imm80 KH53 KH52 nin5; Bam-]
FT                   1. Charon 13A
FT                   cross with
FT                   2. Charon 10
FT                   -> lambda XDR3.24 [lambda b1007 KH54 nin5]
FT                   1. lambda XDR3.24
FT                   cross with
FT                   2. lambda BVC4
FT                   -> Charon 28-2221 [lambda b1007 KH54 nin5; Bam-]
FT                   1. Charon 28-2221
FT                   mutation
FT                   -> Charon 28-2222 [lambda b1007 KH54 nin5 ?; Bam-]
FT                   1. Charon 28-2221
FT                   mutation
FT                   -> Charon 28-2270 [lambda ? b1007 KH54 nin5; Bam-]
FT                   1. Charon 28-2222
FT                   2. Charon 28-2270
FT                   -> Charon 30 [lambda b1007 b1007 KH54 nin5; Bam-]
FT                   1. Charon 30
FT                   -> Charon 30A [lambda b1007 b1007 KH54 nin5; Bam-]
FT                   1. piAN7 SalI-BamHI
FT                   DNA polymerase I:
FT                   2. lambda Sau3A-AluI 18783..19159
FT                   -> phage [SalI at 19159 instead of AluI]
FT                   1. phage, plasmid and SupF
FT                   recombination
FT                   2. Charon 30A, arms
FT                   -> lambda FAC101 [SalI at 19159 instead of AluI]
FT                   1. Charon 30
FT                   cross with
FT                   2. lambda BVC4
FT                   -> Charon 31 [lambda lac5 b1007 b1007 KH54 nin5]
FT                   1. Charon 31 remove EcoRI-EcoRI
FT                   2. mouse liver DNA EcoRI-EcoRI 12000bp [no BamHI,SalI]
FT                   -> Charon 32 [unique BamHI]
FT                   1. Charon 32 BamHI-SalI
FT                   Klenow:Klenow
FT                   -> Charon 33 [unique SalI, no BamHI, in right arm]
FT                   1. pUC13 BamHI 2680bp 261..261
FT                   Klenow
FT                   -> pUC13Bam- 2680bp
FT                   1. lambda 540
FT                   2. E. coli HindIII-HindIII 10000bp, lpp gene
FT                   -> lambda lppEc-1
FT                   1. pSC101 EcoRI 9263bp 9262..9262
FT                   2. lambda lppEc-1 HaeIII-HaeIII 2800bp, lpp gene
FT                   EcoRI linker 8bp ngaattcn:EcoRI linker 8bp ngaattcn
FT                   -> pKEN111
FT                   1. pKEN111
FT                   2. pBR322
FT                   -> pKEN033
FT                   1. pKEN033
FT                   -> pKEN037
FT                   1. pMB9 EcoRI
FT                   DNA polymerase I
FT                   2. lambda h80 lacUV5 cI857 HaeIII-HaeIII 203bp, lacI/Z
FT                   -> pOP203-1
FT                   1. pMB9 EcoRI
FT                   DNA polymerase I
FT                   2. lambda h80 lacUV5 cI857 HaeIII-HaeIII 203bp, lacI/Z
FT                   -> pOP203-2
FT                   1. pOP203-2 HindIII-HaeIII 530bp, lacUV5 promoter
FT                   2. pMB9 EcoRI
FT                   DNA polymerase I
FT                   HindIII
FT                   -> pOP203-3 [unique EcoRI near UV5]
FT                   1. pKEN037
FT                   2. pOP203-3
FT                   -> pIN-II-A1
FT                   1. pUC13Bam- HindIII 2680bp 234..234
FT                   2. pIN-II-A1 HindIII, complete
FT                   -> plasmid2
FT                   1. plasmid2 EcoRI-EcoRI, MCS
FT                   1. Charon 33 EcoRI
FT                   -> Charon 34 [EcoRI-...-HindIII-BamHI-EcoRI]
FT                   1. Charon 34 BamHI
FT                   2. E. coli phage BamHI-BamHI
FT                   -> Charon 35
FT                   1. pUC19 BamHI 2686bp 418..418
FT                   Klenow
FT                   -> pUC19Bam- 2686bp
FT                   1. pUC19Bam- HindIII 2686bp 448..448
FT                   2. pIN-II-A1 HindIII, complete
FT                   -> plasmid
FT                   1. Charon 34 BamHI-BamHI, stuffer [no BamHI, no EcoRI]
FT                   2. plasmid BamHI-BamHI, MCS
FT                   -> phageA
FT                   1. Charon 35 HindIII
FT                   2. human HindIII-HindIII
FT                   -> Charon 35-H668 [no KpnI in stuffer]
FT                   1. Charon 35-H668 KpnI
FT                   2. oligo KpnI-KpnI 12bp tggaggaagtac
FT                   \ 3'catgacctcctt5'
FT                   -> phage2 [no leftmost KpnI in J gene]
FT                   1. phage2
FT                   cycling, remove KpnI at right of KpnI of J gene
FT                   -> Charon 35-H668 Kpn- [no KpnI]
FT                   1. Charon 35-H668 Kpn- left end-EcoRI, left arm
FT                   2. phageA EcoRI-EcoRI, stuffer/MCS
FT                   3. Charon 35-H668 Kpn- EcoRI-right end, right arm
FT                   -> Charon 36 [KpnI]
FT                   1. pUC8 SmaI 2665bp 238..238
FT                   XhoI linker 6bp ctcgag
FT                   -> pUC8Sm/Xh 2665bp [XhoI-EcoRI fragment in MCS]
FT                   1. Charon 33 [no SmaI in stuffer]
FT                   cycling
FT                   -> Charon 33 Sma- [no SmaI in P gene of right arm]
FT                   1. Charon 33 SalI
FT                   2. pUC8Sm/Xh XhoI 2665bp 238..238
FT                   -> phage3
FT                   1. Charon 36 EcoRI, left arm
FT                   2. phage3 EcoRI, right arm [no SmaI, no SalI, no XhoI]
FT                   -> Charon 37
FT                   1. Charon 36 ScaI-ScaI 2400bp 16420..18683, 2 KpnI/J
FT                   2. lambda FAC101
FT                   cycling, remove ApaI in gene G
FT                   ScaI-right end
FT                   2. lambda FAC101 left end-ScaI
FT                   -> lambda FAC101 Apa'Kpn' [no ApaI, unique KpnI]
FT                   1. Charon 37 SalI
FT                   2. pUC8Sm/Xh XhoI 2665bp 238..238
FT                   -> phageB
FT                   1. phageB SalI-right end, right arm
FT                   2. lambda FAC101 Apa'Kpn' left end-SalI, left arm
FT                   -> Charon 38A [SupF]
FT                   1. Charon 38A
FT                   mutation, back to no suppressor
FT                   -> Charon 38
FT                   1. pUC19 EcoRI 2686bp 397..397
FT                   2. adenovirus 2 EcoRI-EcoRI 4295bp 21338..25633
FT                   -> plasmidA 6981bp
FT                   1. plasmidA BamHI-BamHI, MCS to adenovirus
FT                   DNA polymerase I:DNA polymerase I
FT                   2. lac operator
FT                   blunt end:blunt end
FT                   -> fragment
FT                   1. fragment
FT                   BamHI linker 6bp ggatcc:BamHI linker 6bp ggatcc
FT                   BamHI-BglII 540bp, MCS/adenovirs/lac operator
FT                   -> fragment2
FT                   1. fragment2 BamHI-BglII 540bp, MCS/adenovirs/lac oper
FT                   2. pKC30 remove BamHI-BglII, MCS
FT                   -> plasmid2
FT                   1. pUC8 remove SmaI-HindIII 23bp 238..261, MCS/2642bp
FT                   2. oligo HindIII-SmaI
FT                   -> pISD27
FT                   1. plasmid2 BamHI-BglII, MCS/adenovirs/lac oper
FT                   2. plasmid2 BamHI-BglII, MCS/adenovirs/lac oper
FT                   -> polymer [XhoII at junctions]
FT                   1. polymer NaeI-NaeI, MCS/adenovirs/lac oper
FT                   DNA polymerase I:DNA polymerase I
FT                   2. pISD27 HindIII-EcoRI, MCS
FT                   :DNA polymerase I
FT                   -> polystuffer [MCS/polystuffer]
FT                   1. Charon 38A left end-HindIII
FT                   2. polystuffer HindIII-HindIII, MCS/polystuffer
FT                   3. Charon 38A HindIII-right end
FT                   -> Charon 39A
FT                   1. Charon 39A
FT                   recombination
FT                   2. Charon 38
FT                   -> Charon 39 [lacZ]
FT                   1. pBR322 XmaIII 4361bp 940..940
FT                   BglII linker 6bp agatct
FT                   -> pISD34
FT                   1. adenovirus 2 BamHI-BglII 21607..21820
FT                   2. pISD34 remove BamHI-BglII, MCS
FT                   -> plasmidC [BamHI, BglII, unique XmaIII near NaeI]
FT                   1. plasmidC XmaIII
FT                   fill in:fill in
FT                   2. lac operator
FT                   blunt end:blunt end
FT                   -> fragmentA [XmaIII on both sides of lac operator]
FT                   1. fragmentA
FT                   BamHI linker 6bp ggatcc:BamHI linker 6bp ggatcc
FT                   BamHI-BglII 235bp, MCS/adenovirs/lac operator
FT                   -> fragmentB
FT                   1. fragmentB BamHI-BglII 235bp, MCS/adenovirs/lac oper
FT                   2. pISD34 BamHI-BglII
FT                   -> plasmidD
FT                   1. plasmidD BamHI-BglII 235bp, MCS/adenovirs/lac oper
FT                   2. plasmidD BamHI-BglII 235bp, MCS/adenovirs/lac oper
FT                   -> polymerB [XhoII at junctions]
FT                   1. polymerB NaeI-NaeI, MCS/adenovirs/lac oper
FT                   DNA polymerase I:DNA polymerase I
FT                   2. pISD27 HindIII-EcoRI, MCS
FT                   :DNA polymerase I
FT                   -> polystufferB [MCS/polystuffer]
FT                   1. Charon 39 left end-HindIII, left arm
FT                   2. polystufferB HindIII-HindIII, MCS/polystuffer
FT                   3. Charon 39 HindIII-right end, right arm
FT                   -> Charon 40A
FT                   1. Charon 40A left end-EcoRI 19171bp, left arm
FT                   2. Charon 38 EcoRI-right end 9622bp, right arm
FT                   -> Charon 40 28793bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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