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ID   CHARON4A   preliminary; circular DNA; SYN; 30700 BP.
AC   IG7001;
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector Charon4A - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pUC19Bam- from pUC19
RC   plasmid from pUC19Bam- & pIN-II-A1
RC   phage from Charon 34 & plasmid
RC   phage2 from Charon 35-H668 & linker
RC   Charon 36 from phage & phage2
RC   Charon 33 Sma- from Charon 33
RC   pUC8Sm/Xh from pUC8 & linker
RC   phage3 from Charon 33 & pUC8Sm/Xh
RC   Charon 37 from Charon 36 & phage3
RC   lambda FAC101 Apa'Kpn' from Charon 36 & lambda FAC101
RC   Charon 38A from Charon 37 & lambda FAC101 Apa'Kpn'
RC   Charon 38 from Charon 38A
RC   plasmid2 from pKC30 & pUC19 & adenovirus 2 & lac operator & linker
RC   pISD27 from pUC8 & oligo
RC   Charon 39A from plasmid2 & pISD27 & Charon 38
RC   Charon 39 from Charon 39A & Charon 38
RC   pISD34 from pBR322 & oligo
RC   Charon 40A from plasmid2 & pISD34 & Charon 39
RC   Charon 40 from Charon 40A & Charon 38
RA   Dunn I.S., Blattner F.R.;
RT   "Charons 36 to 40: multi enzyme, high capacity, recombination
RT   deficient replacement vectors with polylinkers and polystuffers";
RL   Nucleic Acids Res. 15:2677-2698(1987).
CC   NM (Charon4A)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (lambda)
CC   BR ()
CC   OF (p60B)
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> Charon 4A 30700bp"
FT   misc_binding    19800..19800
FT                   /note="SIT EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;