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ID   CHARONBSM  preliminary; DNA; SYN; 43100 BP.
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AC   ATCC77446;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli lambda phage vector Charon BS(-) - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   Charon BS(-), Charon BS(+) from Charon 40 & M13
RA   Swaroop A.H., Weissman S.M.;
RT   "Charon BS(+) and (-), versatile lambda phage vectors for
RT   constructing directional cDNA libraries and their efficient
RT   transfer to plasmids";
RL   Nucleic Acids Res. 16:8739-8739(1988).
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CC   Charon BS(-) (ATCC 77446) and Charon BS(+) (ATCC 77447) differ in
CC   the orientation of the M13 origin of replication. Vector for
CC   high-efficiency directional cloning.  Clones can be converted to
CC   plasmids by recircularization after a NotI digest. The order of the
CC   major features in this plasmid is: left arm - T3 - NotI/MCS/XhoI -
CC   pMB1 ori - ampR - M13 ori - SacII - NotI - T7 - right arm. [1]
CC   Deposited by: A.H.Swaroop
CC   NM (Charon BS-)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(sequencing)(expression)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Charon 40 28793bp
FT                   2. M13 6407bp
FT                   -> Charon BS(-) 43100bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   misc_binding    0..0
FT                   /note="MCS NotI-BamHI-EcoRI-EcoRV-HindIII-SalI-XhoI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   promoter        0..0
FT                   /note="PRO E. coli lac"
FT   promoter        0..0
FT                   /note="PRO phage T3"
FT   promoter        0..0
FT                   /note="PRO phage T7"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ' gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI phage M13"
FT   rep_origin      0..0
FT                   /note="ORI phage lambda"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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