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ID   CLA12      preliminary; circular DNA; SYN; 70 BP.
AC   M17544;
DT   15-JUN-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate plasmid vector Cla12 - incomplete, adaptor.
KW   cloning vector; retrovirus; helper independent.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-70
RC   pUC12N from pUC12 & linker
RC   327AC from pBR327
RC   327ACR from 327AC
RC   327ACRH from 327ACR
RC   Cla12 from pUC12N & 327ACRH & pBR322
RC   Cla12Nco from Cla12 & pUC12N & L-src eukaryotic initiator
RC   SA Cla12Nco from Cla12 & pUC12N & pr779/1089-989/1079-2795/2795
RA   Hughes S.H., Greenhouse J.J., Petropoulos C.J., Sutrave P.;
RT   "Adaptor plasmids simplify the insertion of foreign DNA into
RT   helper-independent retroviral vectors";
RL   J. Virol. 61:3004-3012(1987).
RN   [2]
RC   pr1089/910 from Rous sarcoma virus SR-A
RC   pr1089/2795 from Rous sarcoma virus SR-A
RC   pr1089/1079 from Rous sarcoma virus SR-A
RC   pr882/910 from Rous sarcoma virus SR-A
RC   pr882/2795 from Rous sarcoma virus SR-A
RC   pr882/1079 from Rous sarcoma virus SR-A
RC   pr779/1079 from Rous sarcoma virus SR-A
RC   pr779/2795 from Rous sarcoma virus SR-A
RC   pr882/989-1089/1079 from Rous sarcoma virus SR-A
RC   pr882/989-1089/989-1089/1079 from Rous sarcoma virus SR-A
RC   pr882/1089-989/1079 from Rous sarcoma virus SR-A
RC   pr882/1089-989/1089-989/1079 from Rous sarcoma virus SR-A
RC   pr779/989-1089/1079 from Rous sarcoma virus SR-A
RC   [pr779/1089-989/1079-2795/2795 from Rous sarcoma virus SR-A]
RC   RCAN or pr779/2795 from ASV SR-A
RC   RCAS or pr779/989-1089/2795 from ASV SR-A
RA   Hughes S., Kosik E.;
RT   "Mutagenesis of the region between env and src of the SR-A strain
RT   of Rous sarcoma virus for the purpose of constructing
RT   helper-independent vectors";
RL   Virology 136:89-99(1984).
RN   [3]
RC   1090/alpha-CHCG from ASV SR-A & alpha-CHCG gene
RC   1090/alpha-HCGL from ASV SR-A & alpha-CHCG gene
RC   1090/alpha-HCGS from ASV SR-A & alpha-CHCG gene
RC   884/-29/alpha-CHCG from ASV SR-A & alpha-CHCG gene
RA   Sorge J., Hughes S.;
RT   "The splicing of intervening sequewnces introduced into an
RT   infectious retroviral vector";
RL   J. Mol. Appl. Genet. 1:547-599(1982).
RN   [4]
RC   plasmid from pBR322 & lambda gtWES-RSV
RC   plasmid2 from pBR322 & plasmid, RSV LTR
RC   plasmid3 from plasmid2 & linker
RC   plasmid4 from plasmid3 & linker
RC   plasmid5 from pBR322
RC   plasmid6 from plasmid4 & plasmid5
RC   plasmid7 from plasmid6 & lambda gtWES-RSV
RC   pr882/-273 from plasmid7 & linker
RC   pr882/-29 from plasmid7 & linker
RC   pr882/-8 from plasmid7 & linker
RC   pr779/-273 from plasmid7 & linker
RC   pr779/-29 from plasmid7 & linker
RC   pr779/-8 from plasmid7 & linker
RA   Sorge J., Ricci B., Hughes S.;
RT   "cis-acting RNA packaging locus in the 115-nucleotide direct
RT   repeat of Rous sarcoma virus";
RL   J. Virol. 48:667-675(1983).
RN   [5]
RC   lambda gtWES-RSV from lambda gtWES series & RSV
RA   DeLorbe W.J., Luciw P.A., Goodman H.M., Varmus H.E., Bishop J.M.;
RT   "Molecular cloning and characterization of avian sarcoma virus
RT   circular DNA molecules";
RL   J. Virol. 36:50-61(1980).
CC   NCBI gi: 209340
CC   NM (Cla12)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (retrovirus)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC12 2680bp, at ATG
FT                   NcoI linker 6bp ccatgg
FT                   -> pUC12N 2680bp
FT                   1. pBR327 HindIII-AvaI 1878bp 1425..3273..30
FT                   Klenow:Klenow
FT                   -> 327AC 1878bp [HindIII]
FT                   1. 327AC EcoRI 1878bp, pBR327 3272..3272
FT                   Klenow
FT                   -> 327ACR 1878bp [no EcoRI]
FT                   1. 327ACR HindIII 1878bp, pBR327 30..30
FT                   Klenow
FT                   -> 327ACRH 1878bp [no EcoRI, no HindIII]
FT                   1. pBR322 HindIII 30..30
FT                   oligo partial_HindIII-EcoRI 7bp agctcgg
FT                   small ClaI-EcoRI 12bp 25..37 cgataagctcgg
FT                   2. pUC12N EcoRI-HindIII 45bp 231..276, MCS
FT                   3. pBR322 ClaI-HindIII 5bp 25..30,
FT                   \ complement HindIII-ClaI 7bp 27..34 agcttat
FT                   4. 327ACRH ClaI 1878bp 25..25
FT                   -> Cla12 1940bp [EcoRI near SspI]"
FT   rep_origin      0..0
FT                   /note="ORI retrovirus"
FT   misc_feature    1..70
FT                   /note="ClaI-EcoRI-HindIII-ClaI"
SQ   Sequence 70 BP; 16 A; 21 C; 18 G; 15 T; 0 other;
     atcgataagc tcggaattcg agctcgcccg gggatcctct agagtcgacc tgcagcccaa